Fatty acid composition of birds and game hunted by the Eastern James Bay Cree people of Québec

Background Indigenous peoples have traditionally relied on foods hunted and gathered from their immediate environment. The Eastern James Bay Cree people consume wild game and birds, and these are believed to provide health as well as cultural benefits. Objective To determine the fatty acid (FA) composition of traditional game and bird meats hunted in the Eastern James Bay area. Design Harvested traditional game and birds were analysed for FA composition. A total of 52 samples from six wildlife species were collected in the areas of Chisasibi, Waswanipi and Mistissini, of which 35 were from birds (white partridge and Canada goose) and 17 were from land animals (beaver, moose, caribou and black bear). Results Alpha-linolenic acid (ALA) was the most common n-3 polyunsaturated fatty acid (PUFA) in all samples except for the black bear flesh, in which it was docosapentaenoic acid (DPAn-3). In white partridge, beaver and caribou flesh, PUFAs (mainly n-6) were the most common category of fats while in goose, moose and black bear flesh, monounsaturated fatty acids (MUFAs) predominated. In all species, saturated fatty acids (SFAs) were the second most important FAs. It would appear that in the land animals and birds that were analysed, the SFA content was lower and the PUFA content was higher than store-bought meats giving them a more heart-healthy profile. Conclusions These results showed that the FA composition of game species consumed by the James Bay Cree population is consistent with a beneficial diet and that traditional foods should continue to be promoted among the Cree people to provide better physical health as well as social and spiritual benefits.

market foods. The availability of market foods has led to a lower contribution of traditional foods to the total dietary energy intake among Eastern James Bay Cree peoples (8).
The nutrient composition of fish species consumed by Northern indigenous peoples is relatively well documented (9,10); these species are known to be naturally rich in omega-3 polyunsaturated fatty acids (n-3 PUFAs) that are protective against cardiovascular disease (CVD) (11,12). However, despite their importance in the traditional Cree diet, only sparse data on fatty acid (FA) composition are available for terrestrial and avian game species. Therefore, documenting FA composition of frequently consumed game species is important to evaluate the dietary FA sources and consequently their contribution to the blood FA profile measured in the inhabitants of Eastern James Bay Cree region.
Thus, in this study, we analysed wild bird and land mammal species frequently harvested and consumed by the Cree residents of the Eastern James Bay Territory in order to characterize their FA composition.

Methods and materials
The project was undertaken as part of the Multi-community Environment and Health Study in Iiyiyiu Aschii survey carried out in the Eastern James Bay Cree population, a joint project of the Cree Board of Health and Social Services of James Bay (CBHSSJB), Laval, McGill and McMaster Universities (13Á15). Previous research agreements were in place with the Cree Nations of Chisasibi, Waswanipi and Mistissini, and local authorities were informed of the data collection.

Study area
Field research to collect samples of wildlife species was conducted in 2011, 2012 and 2014 in the areas of Chisasibi, Waswanipi and Mistissini, three of the nine Eastern James Bay Cree communities. Waswanipi and Mistissini are inland communities located in the south-eastern James Bay Territory, whereas Chisasibi is located further north on the eastern coast of the James Bay (Fig. 1). The Eastern James Bay territory, called Eeyou Istchee, is located between the 49th and the 55th parallel in the province of Québec (Canada) and covers more than 350,000 km 2 . In 2011, the total indigenous population of the Eastern James Bay territory was 16,010 (16).

Sample collection
A total of 52 samples from six wildlife species were collected, of which 35 were birds (white partridge and Canada goose) and 17 were land animals (beaver, moose, caribou and black bear). The parts of the animals that were sampled were those usually consumed by the Eeyouch (Cree people).
Local research assistants were trained in the safe handling of harvested meats using information from elders, the Ministry of Agriculture and Fisheries and Food of the province of Québec (17). Sampling took place in the homes of hunters and participation was voluntary. Samples were collected from fresh or frozen kills, and the animals and the precise location of those samples on the carcasses were randomly selected. Once collected, samples were identified and stored at (208C for delivery to the Lipid Laboratory of the ''Centre Hospitalier de l'Université Laval'' for analysis. The skin and flesh of the partridge and geese were separated to allow a separate assessment of their FA content. Flesh, fat, skin and organ meats such as liver, heart, kidney, stomach and eggs were analysed.

FA analysis
Samples were analysed individually for FA composition. Most samples were raw except for two samples of caribou muscle and fat, which were smoke dried, and three samples of rendered bear fat. Rendered bear fat is subcutaneous fat that is melted down after which all flesh is removed and frozen for storage.

Statistical analysis
Means and standard deviations were calculated using Microsoft Excel and SAS 9.2 (2008, SAS Institute Inc., Cary, NC).

Results and discussion
White partridge, beaver and caribou flesh had higher quantities of PUFAs (mainly n-6), whereas goose, moose Francoise Proust et al. and black bear flesh had monounsaturated fatty acids (MUFAs). PUFA proportions ranged from 44 to 57% of total FAs when PUFAs predominated and MUFAs were in the range of 41 to 55% when MUFAs predominated, while saturated fatty acids (SFAs) accounted for approximately 30Á40% of total FAs in all the species analysed (Tables I and II). In all species, SFAs were the second most common FAs.
Palmitic acid (16:0) was the most common SFA except in caribou where the longer chain stearic acid (18:0) prevailed. Oleic acid (18:1) was the most common MUFA. ALA (18:3n-3) was the most common n-3 PUFA in all samples except for the black bear flesh where DPAn-3 was the most common FA. Overall, total n-3 PUFA levels were low except in the fat samples of the land mammals and the fat and skin samples of the wild birds. Linoleic acid (LA) (18:2n-6) was the most common n-6 PUFA, and the n-6 PUFAs were the predominant PUFA in all samples.

Fatty acid composition of birds and game
Because traditional game and birds are usually replaced by store-bought meats, we compared the total SFAs in the wild birds to chicken and the land mammals to beef as characterized on the Canadian Nutrient File (26). Total SFAs were lower in land mammal flesh than beef (0.30Á0.46 g/100 g vs. 4.33 g/100 g) (26). In the pure fat, this was not so clear: there was 49.8 g of SFAs in beef compared with a range of 37.3 g in beaver to 75.5 g in bear. Total MUFA was lower in the wild mammals versus beef, and total n-3 PUFA was higher. In wild birds versus chicken, total SFAs (0.51Á1.55 g vs. 2.20 g/100 g), MUFAs (0.11Á2.04 g vs. 2.73 g/100 g) and PUFAs (0.70Á1.13 g vs. 2.18 g/100 g) were lower in partridge and goose than chicken. For the skin, partridge had less SFAs, MUFAs and PUFAs than chicken, whereas goose had more. Similar to store-bought foods such as beef and chicken (26), trans FAs were present in small amounts. ALA and total n-3 were much superior in the wild birds. Overall, we can conclude that wild birds and game appear to be better sources of n-3 FAs than store-bought meats.
n-3 PUFAs have many physiological properties that would appear to decrease the risk of CVD and have been shown to do so in observational studies (12,27). Consumption of ALA has been associated with the reduction of risk and death from CVD in older adults and men in prospective cohorts (27,28). In addition, a meta-analysis showed consistent reduction of CVD with dietary ALA (29). In a pooled analysis of cohort studies, Jakobsen et al. found that replacing SFAs with PUFAs was beneficial for cardiovascular health, but MUFAs show no effect (30). Therefore, since SFAs were lower and PUFAs higher in the wild game and birds, replacing storebought meats with wild game and birds could improve CVD health. Also, recent evidence confirmed the benefits of n-6 PUFAs as well as n-3 PUFAs (31Á33).
We acknowledge that a more stringent sampling process and a greater number of samples would yield more representative results. The mixture of cooked and raw samples was another limitation of our study. However, other authors have found very low intraspecies variation ( B5%) of PUFA content with gentle cooking that indicated limited intramuscular fat in game species (34). To our knowledge, this is the first time that samples of game meat from this region have been analysed for FA content, and the limitations of our study do not prevent the use of these rare species FA values.

Conclusion
These results add information on FA profiles of foods that have not been previously studied. We have shown that the FA composition of game species harvested and consumed by the Eastern James Bay Cree people may contribute to a healthy diet due to their high proportions of n-3 PUFAs. Traditional wildlife foods such as the game species samples analysed in this study appear to be better choices as compared to lower quality store-bought foods.
Traditional foods also have cultural importance in addition to being good sources of high-quality protein and essential nutrients. For example, the Canada goose harvesting season, a cultural tradition that provides the Cree people with the opportunity for passing essential traditional knowledge to the next generation (20) helps ensure the continuation of social and cultural identity of the Eastern James Bay Cree.
A previous study carried out in seven James Bay Cree communities showed that older adults consumed traditional food more frequently than younger age groups (13Á15, 35). Concurrent with decreased traditional food intake in this indigenous population, diabetes and CVD have increased in alarming proportions (16). Hence, the consumption of traditional foods especially wild game and birds should continue to be promoted among the Cree population, particularly among younger individuals who are less likely to consume traditional foods.
Finally, this study gives the Cree communities a better understanding of some of the nutritional benefits of their traditional food species. This could aid health practitioners in the development of nutritional health promotion materials. In the future, further analysis of contaminants and persistent lipophilic pollutants and heavy metals such as mercury in these species is needed to better understand and mitigate the risks posed by possible environmental threats although this should always be balanced against the harm of replacing these nutrient-rich foods by store-bought foods. this article. This scientific communication is a report from the Nituuchischaayihtitaau Aschii: Multi-Community Environment and Health Study in Eeyou Istchee supported by the Cree people of Northern Québec, the Cree First Nations and the Cree Board of Health and Social Services of James Bay through financial contributions from Niskamoon Corporation.