Unnoticed introductions, a silent threat for biodiversity: island endemic Ladigesocypris ghigii (Actinopterygii, Leuciscidae) translocated to continental Greece

Abstract Morphological similarity between an intruder and a native species can hinder the detection of the intruder. The undetected non-native species will thus have more time to affect the local ecosystem and spread. This paper describes an instance of unnoticed introduction of a fish species morphologically resembling a native endemic species. We report on the first established population of the Rhodes minnow, Ladigesocypris ghigii (Actinopterygii: Leuciscidae), endemic to Rhodes Island, which has been recently introduced to a river near Athens, mainland Greece. When it was first noticed, in 2017, the species was misidentified due to its morphological similarity to Pelasgus marathonicus, the only native leuciscid of the streams and rivers around Athens, until it was subject to genetic and detailed morphological examination. Genetic analyses based on both mitochondrial (cytochrome b) and nuclear (recombination activating gene, RAG1) markers unambiguously identified the collected individuals and provided information on the relationships of Ladigesocypris at both the species and generic level. Our investigation supports that L. ghigii was recently established near Athens, and that the population probably originated from Rhodes Island, an eastern Aegean island. In 2021, a high-density thriving mainland population coexisted solely with the native European eel (Anguilla anguilla) along at least 9.7 km of river habitat. It was assessed as being of high invasive risk for the region where it was established by the Aquatic Species Invasiveness Screening Kit (AS-ISK) screening tool. We propose a feasibility study be undertaken to investigate the application of measures that may include actions to exterminate or contain the population. Special attention is required since the species is considered vulnerable in its native range, while the translocated population coexists with native biota within a stream ecosystem of outstanding local conservation value.


Introduction
Freshwaters are among the ecosystems most impacted by biological invasions (Bampfylde et al. 2010).At the same time, invasive species are one of the main causes of extinction among native freshwater species (Miller et al. 1989;Witte et al. 1991) and one of the main threats to them in many areas (Economidis 1995;Dextrase & Mandrak 2006;Bogutskaya et al. 2012).Fish introductions have figured especially prominently on a global scale (Helfman 2007).Along with widespread evidence of the negative impacts of freshwater non-native fishes, the necessity to develop a mode to assess the "real threat" of non-native fish introductions arose (Gozlan 2008).Since most non-native freshwater fish are often impossible to control once released, assessing the impacts and threats of introductions has garnered high interest in recent years (Vitule et al. 2009;Tarkan et al. 2017).
Non-native freshwater fish species are often relegated to two distinct categories: aliens (hailing from other countries, other continents or distant biomes) and translocated non-natives (Economidis et al. 2000;Copp et al. 2005;Koutsikos et al. 2019;Boon et al. 2020).The latter category involves intra-country human-assisted fish transfers concerning species translocated out of their native range but within the same country or within the same region (Helfman 2007;Boon et al. 2020).Most freshwater researchers treat non-indigenous translocated species as having the same level of importance as other alien fish species (Helfman 2007;Tarkan et al. 2017); however, sometimes there is confusion or doubt regarding the geographical origin of the translocated species (e.g.Zogaris & Apostolou 2011).Also, members of the public and policy stakeholders may not have the natural history knowledge to consider these seemingly "native fishes" an invasive threat (Vardakas et al. 2022).It is well known that some translocated species can become invasive, producing severe deleterious effects on native species or ecosystems (Perdikaris et al. 2016;Koutsikos et al. 2019).
In this paper, we report on the overlooked translocation and establishment of the Rhodes minnow Ladigesocypris ghigii near Athens, Greece, due to its morphological similarity to a native species -Pelasgus marathonicus, the Marathon minnow.The Rhodes minnow (also known as the Ghizani) is a threatened species endemic to the eastern Aegean Island of Rhodes (Greece), assessed by the International Union for Conservation of Nature (IUCN) as Vulnerable (Crivelli 2006).Rhodes has a very different biogeographical history to the mainland European part of Greece, being a part of the wider western section of the Anatolian peninsula, belonging to the Western Anatolia Freshwater Ecoregion (Abell et al. 2008).Although there have been many freshwater fish introductions from Asia to Europe (Muñoz-Mas et al. 2023), this is the first case of intra-country freshwater fish translocation from Asia to Europe in the Mediterranean region.
Mainland Greece is inhabited by a diverse native freshwater fish fauna, with a high proportion of endemic species (Barbieri et al. 2015).The high endemism has resulted from the complex geological history of the area, which affected the evolution of the hydrological network and enabled local speciation during long stretches of geological time (Buj et al. 2019;Viñuela Rodríguez et al. 2021).In the region, the Leuciscidae (sensu Schönhuth et al. 2018) is the most speciose family.Some leuciscid species, e.g.members of the genus Pelasgus, adapted to small streams and wetlands (Kottelat & Barbieri 2004), may be particularly susceptible to human-induced threats; this includes Pelasgus marathonicus confined to the Western Aegean Ecoregion in Greece (Kottelat & Freyhof 2007).The genetic structure of populations of P. marathonicus from separated drainages, even those located close to each other, may be distinctive and unique, deserving conservation attention at the population level (Viñuela Rodríguez et al. 2020;Tsaparis et al. 2021;Vlami et al. 2021).Since 2017, rumours of small fish found in a stream near Rafina, east of the capital city of Athens (Attika Region), lying in proximity to the native range of P. marathonicus (Viñuela Rodríguez et al. 2020;Tsaparis et al. 2021), were loosely attributed to this native species (Zogaris 2019).SZ and colleagues first photographed the young fish near the town of Rafina in 2017 and identified them tentatively as Pelasgus, not considering the possibility of an introduction of a similar-looking leuciscid minnow.Upon re-inspection a few years later, the unusual case of a translocation of the Rhodes minnows was discovered.This confusion was detrimental; for a short while it was assumed that there was one more population of the locally scarce P. marathonicus near Athens (Hellenic Centre for Marine Research (HCMR) data), and the Rhodes minnow had time to establish a large population at the locality of initial documentation.
Here, we discuss identification details, provide evidence for the establishment of the translocated population, and assess the risk of invasiveness of the species in the region of translocation.We further provide indications and suggestions on how this introduction may have taken place and the relevant conservation implications.

Sampling and methods for morphological counts and measurements
Since 2017, small leuciscid fish have been observed in a stream near the port town of Rafina (38°01′14″ N, 24°00′17″E), a coastal settlement 25 km east of Athens and very close to Athens International Unnoticed introductions, a silent threat for biodiversity Airport, but were considered native Pelasgus marathonicus.In the autumn of 2021, several samples were collected after unusually large specimens for P. marathonicus (around 10 cm) had been photographed and posted on a relevant citizen science Facebook group (see Acknowledgements).Finclips of four individuals were collected for analysis and careful photographic comparison immediately suggested that the population was not the assumed native P. marathonicus (Figure 1).
Two standardised survey samplings of the fish population along stream reaches were undertaken in April 2022.Collecting was conducted under Hellenic Ministry of Environment licence no.143634/1894/20-7-2016 within water bodies monitored by HCMR, with the approval of the Ethical Committee.Sampling consisted of electrofishing, following approaches used through the European Union Water Framework Directive (EU WFD).Sampling was conducted at a river stretch demarcated by physical boundary features to minimise fish escape during electrofishing (e.g.riffle areas, changes in instream habitats, natural or artificial barriers).A high-quality battery-powered backpack electrofishing unit was used (Smith Root 24 L DC pulsed, 1.5 kW, 35-100 Hz, max 980 V).At each site, a single pass of a river stretch at least 90 m long was completed (average fishing time was 55 min), providing an initial estimate of population density.Fish were moved into buckets, identified to the species level in the field, counted, and measured to size-class intervals, and most of them were released alive back to the river.Visual inspection from shore (using binoculars) and underwater video using a mounted action camera (GoPro Hero 8) at several other locations confirmed the local distribution of the species in the river.
Ten selected specimens were collected for lab inspection.The fish collected for morphological description were caught by electrofishing, euthanised by over-anaesthetisation and fixed in 10% neutral buffered formalin.Methods for meristic counts and measurements followed Barbieri et al. (2017).Measurements were made point to point, with an electronic calliper, to the nearest 0.1 mm.Lateral line pored scales were counted from the

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S. Zogaris et al. anterior-most pored scale (beginning from the first scale adjacent to the operculum).Midlateral row scales were counted from the anterior-most pored scale to the one at the end of the hypural complex.Scales on the caudal fin itself are indicated by "+" and are presented only in the text.Transverse scales are counted as the number of longitudinal scale rows.The scale row on dorsal and ventral midlines is noted as " 1 / 2 ".The last two branched rays articulated on a single pterygiophore in dorsal and anal fin are counted as "1 1 / 2 ".

DNA laboratory procedure and data analyses
Tissue samples for molecular analysis (fin clips) were preserved in 96% ethanol.Genomic DNA was extracted with the Geneaid Genomic DNA Mini Kit (Tissue) (Geneaid Biotech, Taiwan).Two markers were amplified by polymerase chain reaction (PCR): one mitochondrial, cytochrome b gene (cyt b), and one nuclear marker, recombination activating gene (RAG1).Cyt b has been shown to be a reliable marker for fish phylogeny and species identification (e.g.Freyhof et al. 2006;Perea et al. 2010;Buj et al. 2019;Viñuela Rodríguez et al. 2021).RAG1 was chosen as an often-used nuclear marker, useful especially in a higher-level fish phylogeny (e.g.Perea et al. 2010;Schönhuth et al. 2018;Buj et al. 2020).Moreover, cyt b and RAG1 are the most widely used genetic markers for the Leuciscidae (sensu Schönhuth et al. 2018).Furthermore, published sequences of these markers of L. ghigiii and other reference species used in phylogenetic reconstructions were available.Using both mitochondrial and nuclear markers is important to detect possible hybridisation events (e.g.Freyhof et al. 2005;Perea et al. 2016;Buj et al. 2020;Viñuela Rodríguez et al. 2021).PCRs were performed using PPP Master Mix (Top-Bio, Czech Republic).Cyt b was amplified as described in Šanda et al. (2008), while RAG1 amplification followed Viñuela Rodríguez et al. (2021).Sequencing was performed by Macrogen Inc. (Amsterdam, The Netherlands) using internal primers indicated in Viñuela Rodríguez et al. (2021).Apart from the specimens from Rafina, samples of Ladigesocypris irideus from Turkey were also included in molecular analyses (Table I).
The dataset was complemented by published cyt b and RAG1 sequences of Ladigesocypris (Table I).To test the phylogenetic placement of Ladigesocypris and possible recent hybridisation with Pelasgus, sequences of selected species of the genera Squalius, Petroleuciscus (i.e.genera closely related to Ladigesocypris) and Pelasgus (i.e. the leuciscid genus that inhabits the nearby rivers) were included in the analyses (Table I).Sequences of the genus Phoxinus (Schönhuth et al. 2018) were used as outgroup for phylogenetic analyses.
DNASP v. 6.12.01 (Rozas et al. 2017) was used for sorting Ladigesocypris haplotypes (Table I) and for phasing the RAG1 nuclear sequences.In the case of one sequence (sample LEGH4), which included two heterozygotic positions, the probability of phasing was not high (<0.7),so we used all four resulting allele variants of the phased sequence to test the relationships of the haplotypes in the analysis of haplotype network reconstruction.
PARTITIONFINDER2 (Lanfear et al. 2017) was used to determine the best-fitting nucleotide substitution model for each subset of positions inside codons of the protein coding markers on the basis of the Bayesian information criterion (BIC).The following partitions and models were determined and used for the phylogenetic reconstruction: K80+I+G, TRN+I and GTR+I+G for the first, second and third codon position of cyt b, respectively.For RAG, the model JC +I was applied for the first and second codon position, and SYM+G for the third codon position.
Phylogenetic trees based on individual markers were constructed using Bayesian inference (BI) in MRBAYES v. 3.2.6 (Ronquist et al. 2012).For RAG1, unphased data were used.Two independent runs of four Markov chain Monte Carlo (MCMC) simulations were simultaneously run for 1 million generations with sampling trees every 1000 generations.The convergence of the runs was analysed and visualised in TRACER v. 1.7.1 (Rambaut et al. 2018).The first 25% of trees obtained were discarded as "burn-in".Posterior probability indicates branch support.The 50% majority rule consensus trees were visualised using FIGTREE v. 1.4.4 (Rambaut 2018) and edited with INKSCAPE v. 0.92.3 (Inkscape Project, https://inkscape.org).
Haplotype networks were constructed with TCS v. 1.21 (Clement et al. 2000) applying statistical parsimony estimation (Templeton et al. 1992) with a 95% connection limit.For RAG1, phased data were used.Visualisation and editing of haplotype networks were done in the web-based program Unnoticed introductions, a silent threat for biodiversity TCSBU (TCS Beautifier; Santos et al. 2016) and INKSCAPE v. 0.92.3 (Inkscape Project, https:// inkscape.org).

Risk screening
The Aquatic Species Invasiveness Screening Kit (AS-ISK v2.3.3)(Copp et al. 2016), a decision-support tool, was utilised to assess the invasiveness risk of L. ghigii in the Attika Region, an area designated for risk assessment (RA), with the screening carried out by AST and SZ.This tool aligns with the "minimum standards" (Roy et al. 2018) outlined in the European Commission Regulation on the prevention and management of invasive alien species, making it a reliable choice for evaluating non-native species.Its effectiveness has been demonstrated in accurately screening potential invasive non-native aquatic organisms in various RA areas worldwide (Vilizzi et al. 2021).
The AS-ISK screening protocol consists of 55 questions (Copp et al. 2016).The initial 49 questions focus on the Basic Risk Assessment (BRA), examining the biogeographical and biological aspects of the species under assessment.The remaining six questions pertain to the Climate Change Assessment (CCA), requiring the assessor to evaluate how future climate conditions might 844 S. Zogaris et al. influence the risks associated with the species' introduction, establishment, dispersal and impact.Each question in the screening requires a response, a level of confidence in the response, and a justification.After completing the screening, the species is assigned a BRA score and a BRA+CCA (composite) score, ranging from −20 to 70 and −32 to 82, respectively.Scores below 1 indicate a low risk of invasiveness, while higher scores classify the species as posing either a medium or a high risk.The distinction between medium and high risk levels is determined by a predefined "threshold" value.In this study, the threshold is based on the calibrated BRA score of 15.25 for translocated and traded aquarium freshwater fishes in Greece (Perdikaris et al. 2016).
The AS-ISK utilises confidence levels associated with each question-related response, ranked as follows: 1 = low, 2 = medium, 3 = high and 4 = very high.These confidence rankings align with those recommended by the Intergovernmental Panel on Climate Change (IPCC 2005).Overall confidence levels (CL Total ), as well as CL BRA and CL CCA , are calculated based on the allocated confidence level for each response across all 55 questions.

Genetics
Both analyses of cyt b (1140 bp) and RAG1 (1442 bp) identified specimens from Rafina as Ladigesocypris (Figures 2 and 3).There are no traces of recent hybridisation with Pelasgus native to the investigated region (Figure 2).All Ladigesocypris specimens bore solely alleles falling into the clade of this genus on both markers used (Figure 2).The cyt b resolved Ladigesocypris as an internal lineage within Squalius, with Petroleuciscus borysthenicus as the sister lineage to Squalius/ Ladigesocypris (Figure 2), while RAG1 placed P. borysthenicus, Squalius and Ladigesocypris in a polytomy (Figure 2).All sequences of Ladigesocypris are very similar among themselves (max.5 mutation steps on cyt b, and 2-3 on RAG1, see Figure 3).There is no haplotype sharing between the localities/areas (Rafina, Rhodes and the Turkish mainland) (Figure 3).In terms of cyt b, the Rafina population is more similar to the Rhodes population, while in terms of RAG1 it is more difficult to identify relationships, as all haplotypes are more similar among themselves (Figure 3).

Morphology
The collected Ladigesocypris (up to 71.5 mm in standard length (SL)) included individuals that were considerably larger than P. marathonicus (max.reported SL 56 mm, Kottelat & Barbieri 2004), but despite this fact, the overall cursory structure of the two minnows is rather similar (Figure 1).
Ladigesocypris ghigii is a small-bodied freshwater leuciscid (max SL: female 90 mm; male 65 mm) distinguished from other Leuciscidae in south-eastern Europe, and especially from P. marathonicus, by the following combination of characters: incomplete lateral line with 11-17 perforated scales (vs 0-7 in P. marathonicus); 27-33 total scales in midlateral row (vs 36-41 in P. marathonicus); preserved specimens have a broad midlateral stripe from head to caudal base, separating the dark brown back from the white belly (vs missing in P. marathonicus) (Kottelat & Freyhof 2007).In living specimens, the shiny golden-silver body is slightly interrupted by this line.

Fish assemblage at the surveyed sites
The river water bodies investigated in spring were rather shallow (mean depth 0.35 cm), with perennial flow and a mean wetted width of 3.8 m during spring flow.Recorded densities of L. ghigii were high, ranging from 0.73 to 1.1 individuals per square metre (a total of 455 specimens were collected at two separate sites).In most areas the fish formed schools in pooling water areas (in pools, in deep runs and under bridges) and a full arc of size classes was found.The numbers of fish were fairly high in a variety of habitats, including swampy edges, rocky areas and undercut banks, and less so in faster flowing riffles.No Pelasgus marathonicus were recorded.The only other fish species present Unnoticed introductions, a silent threat for biodiversity

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S. Zogaris et al. was the European eel (Anguilla anguilla), also with rather high densities, ranging from 0.04 to 0.06 per square metre (27 individuals recorded in total, across both surveyed sites).Underwater camera footage and visual observation from shore confirmed the species' occurrence at the other two locations and provided a minimum river distribution for the species of 9.7 river kilometres in the spring of 2021.A river barrier (road-crossing) prevents fish from moving upstream at one of the stream tributaries (this was documented using underwater video, above and below the barrier).
We intentionally withhold precise geographical positions of all surveyed stream segments here so as to not encourage amateur aquarists and the wider public to find and illegally collect the fish.

Risk screening
Based on the calibrated threshold values, the BRA scores for L. ghigii indicated a high-risk category for the Attika region, with a score of 32 (Table IV).
When considering the potential impact of climate change, the BRA score increased to 38, indicating an even higher risk for the species to become invasive in this RA area under predicted climate change conditions (Table IV).Several factors and traits contributed to the increase in the BRA score, such as rapid reproduction, persistence, tolerance and resource exploitation.Conversely, the absence of cultivation of the species in the RA resulted in a decrease in the overall score.
The mean confidence levels (CL) associated with responses to the BRA, CCA, and BRA+CCA questions were as follows: CL BRA = 2.25 ± 0.06, CL CCA = 2.00, and CL TOTAL = 2.22 ± 0.06.These values indicate medium to high confidence in all cases.

Phylogenetic and taxonomic implications
The newly established leuciscid fish population in Attika was identified as L. ghigii/irideus.The relationships of the population from Rafina in Attika with those from Rhodes (L.ghigii) and the Turkish mainland (L.irideus) are not completely clear based on the genetic data.However, the results for mitochondrial cyt b, which is considerably more polymorphic than nuclear RAG1, suggest a higher affinity to the Rhodes Island haplotypes, and thus point to Rhodes as the probable origin of the studied introduced population.
The native range of the genus Ladigesocypris includes inland waters of south-western Anatolia and Rhodes (Kottelat & Freyhof 2007;Çiçek et al. 2020) in the Western Anatolia Ecoregion (Abell et al. 2008).However, the taxonomy at the species level as well as at the genus level is not fully resolved (Giannetto et al. 2019), and there is great confusion and inconsistency in the nomenclature.Further work is needed to clarify whether Ladigesocypris is a separate genus or whether its species belong to Squalius.Three species are generally recognised, L. mermere from the Gediz River basin (Marmara Lake, Turkey) (Çiçek et al. 2020); L. ghigii, suggested to be endemic to the Greek Rhodes Island (Kottelat & Freyhof 2007); and L. irideus from the rivers and streams in the south-western Anatolian mainland, from Küçük Menderes to Dalaman (Yılmaz et al. 2015).Nevertheless, in both the Eschmeyer's Catalog of Fishes and FishBase, L. ghigii is listed as Squalius ghigii, while the other two species are listed as belonging to the genus Ladigesocypris.Moreover, Bogutskaya (1996) considered L. ghigii and L. irideus identical based on morphology, with only very minor differences between the Rhodes Island and Anatolian mainland populations, while she recognised L. mermere as a subspecies, with considerable morphological difference, notably a much higher number of lateral line scales.Subsequent genetic investigations provided similar results for L. ghigii and L. irideus, which were found to be genetically almost identical (Perea et al. 2010;Geiger et al. 2014), although only a small number of specimens were included in both studies.Our results also confirmed very similar haplotypes of L. ghigii and L. irideus, for both mitochondrial and nuclear markers (Figures 2 and  3).Furthermore, Geiger et al. (2014) analysed specimens identified as L. mermere and found they were genetically identical to Petroleuciscus smyrnaeus.Here we provisionally accept a two-species concept for L. ghigii and L. irideus, as a recent speciation event cannot be excluded, and keep the generic name Ladigesocypris while awaiting further comprehensive revision of the Ladigesocypris/Squalius/Petroleuciscus relationships.

Evidence for human-induced translocation
Until now, L. ghigii was known to be confined to its native island of Rhodes which is part of the Western Anatolia Freshwater Ecoregion (Abell et al. 2008).
Along with other nearshore eastern Greek islands, Rhodes forms part of this Asian ecoregion that has been isolated from the European side of the Aegean at least since the Miocene (Zogaris et al. 2009).A population of this genus anywhere west of the Mid-Aegean trench would certainly be presumed translocated by humans.
Recent reviews of the Greek ichthyofauna do not include Ladigesocypris in the area where it was found near Rafina (Economou et al. 2007;Barbieri et al. 2015).The evidence that stream and nearby areas were surveyed is given in Economou et al. (2007), so the species must have been introduced relatively recently.
An internet search produced anecdotal information of an amateur aquarist's breeding of Rhodes minnows in Attika after 2009 from stock brought illegally from Rhodes, published on the Greek Aquarists Board internet forum (http://www.aquatek.gr/).The amateur aquarist forum member specifically states that minnows were originally brought to an Athenian home aquarium from Rhodes via a commercial airplane flight in 2009.Although the aquarist had husbandry experience, only a single couple survived the flight and bred in captivity.Although there are several follow-up posts in the forum, in 2014 the hobbyist confessed that he was "quitting the hobby" and would like to part with the fish (he had "50-60 specimens to give for free").Although we do not have firm evidence of aquarists being the culprits in this invasion, the timing of the alleged transport of these specimens from Rhodes matches the assumed time of their release to the stream near Rafina as no leuciscid species was present in the stream at the time of the survey of Economou et al. (2007).Based on available documentation, it is possible that the species was introduced to Attika between 2014 and 2016, but not before 2010 (HCMR data from wetland surveys in Attika).
Vulnerability to invasion by translocated species is higher in ecosystems near metropolitan megacities such as Athens (Koutsikos et al. 2021).Recently, several translocated reptile species have established populations in and near this city (e.g.Christopoulos et al. 2022).Finally, aquarium hobbyists have been targeted as one of the main vectors for alien fauna transports in Greece (Papavlasopoulou et al. 2014).Since perennially flowing river habitats are scarce around Athens, this system very near Athens, including its international airport and major port facilities, could be vulnerable to human-induced translocations.

Evidence for establishment
After a careful inspection and sampling in 2021 and 2022, we confirm that the species is well established and widespread in at least 9.7 km of the small lowland river near Rafina.Estimated population densities were rather high in spring, and are estimated to be even higher when flow and water levels drop in the summer and autumn (Barbieri et al. 2003).A variety of size classes are present, and some individuals reached maximum recorded sizes for the species.Stream conditions in Attika are remarkably similar to those of many lowland streams on Rhodes Island.Habitat for reproduction is suitable at both surveyed sites, as well as in other parts of the river.Artificial barriers seem to restrict further upstream dispersal, supporting the assumption that the population is recently established.A delay in identification of the species presumably allowed L. ghigii to establish and spread along the river.

Threats to biodiversity
Ladigesocypris ghigii is a strongly opportunistic generalist but it has never been subject to assessment of invasiveness threat in Greece (Perdikaris et al. 2016).
Our species-specific risk screening for potential invasiveness in the Attika region through the AS-ISK tool yielded a high risk of being invasive.The species has been called an "opportunistic" breeder, reproducing under various conditions in the wild and easily in captivity as well (Stoumboudi et al. 2002;Poncin et al. 2005).It is known to undertake mass movements to enter more suitable habitats as summer water levels drop (Kondylatos et al. 2014) and is tolerant of varied conditions for feeding and survival.It survives drought periods in remnant river pools under harsh summer droughts with rapid population comebacks (Mamuris et al. 2005; SZ personal observation).Moreover, within the species' native island, successful human-mediated translocations to other streams have been made (Economidis et al. 2000), and the species may take up a variety of freshwater habitats including lotic and artificial lentic ecosystems.
During the last three decades, the numbers of translocated fish species in Greece have rapidly increased (Economidis et al. 2000;Leonardos et al. 2007;Koutsikos et al. 2012;Viñuela Rodríguez et al. 2021) and the issue is considered a problem in many other European countries as well (Jelić et al. 2016;Tarkan et al. 2017;Vukić et al. 2019;Koutsikos et al. 2021;Marić et al. 2022).There is also evidence that some translocated freshwater fishes may have a much more invasive ability than other aliens, since they may easily adapt to the roughly similar environmental conditions because they originate from neighbouring areas (Koutsikos et al. 2019).
The Rhodes minnow is a fairly attractive small-sized fish, surviving well in small aquarium tanks, and it may be sought out by amateurs who want to develop so-called biotope aquaria with "native fish" in their homes.Although it is not considered an ornamental species, small-bodied fish (usually under 10 cm TL) that survive well in home aquaria are known to be especially problematic invasive threats in other regions (e.g.Florida, see Lawson et al. 2015).
Since the said leuciscid is a small fish, similar in appearance to other native schooling minnow-like fishes, the spread may potentially be undetected for some time.The initial misidentification of translocated fish by local researchers is not uncommon; such introductions may occur more frequently than is currently appreciated, since genetic analyses are not widely used to confirm field identifications (Tellier et al. 2023).Translocated fish spread could also gravely hamper biodiversity conservation efforts by creating confusion and loss of the identity and distinctive values of endemic-rich inland water bodies.In our study region, very close to the invaded river system near Rafina, protected Natura 2000 river sites host endemic P. marathonicus populations of high conservation value.The conservation value of a protected area may decline if the site becomes infested by nonnative fish; this may affect the ecosystem services provided by the protected areas, including various cultural ecosystem services (Vlami et al. 2021).
Finally, translocated non-native freshwater species can present direct and indirect pressures and threats to native species and ecosystems in a variety of ways.This includes spreading of diseases and parasites, examples being the well-known cases of chytrid fungus Batrachochytrium dendrobatidis, a pathogen responsible for the global decline of amphibians (Fisher & Garner 2007); crayfish plague, Aphanomyces astaci, seriously affecting native crayfish species (Mrugała et al. 2017;Svoboda et al. 2017); or Anguillicola crassus, a parasitic nematode severely affecting European eel populations (Cesco et al. 2001), spread by non-native species.However, only very limited information is available about parasitation of Ladigesocypris (Nejat et al. 2023), and the potential risk for spread of parasites and diseases needs further investigation.Another serious potential 850 S. Zogaris et al. threat is hybridisation with related fish species, which can lead to the loss of genetic integrity of the native species (Fumagalli et al. 2002;Viñuela Rodríguez et al. 2021;Marić et al. 2022).Hybridisation in leuciscids, even among different genera, is widely documented (e.g.Bianco 1988;Freyhof et al. 2005;Perea et al. 2016;Buj et al. 2020;Curto et al. 2022).Ladigesocypris was reported to hybridise with Squalius in Turkey (Durand et al. 2000).Fortunately, L. ghigii was introduced into a stream with no native leuciscids, although the nearby rivers are inhabited by the endemic P. marathonicus, with which L. ghigii could hypothetically hybridise.

Recommendations
This paper reports on the recent introduction of a fish species from the Western Anatolia to the Western Aegean Freshwater Ecoregion on the European continent, which was assessed as posing a high risk of invasiveness.We may presume that eradication from the specific water bodies and population control by suppression (e.g. through removal programmes) might help prevent its further spread.Such organised conservation and public communication actions might also help inform the public of the severe and rising fish translocation problem (Britton et al. 2011).Several carefully orchestrated eradication attempts have been promoted in Europe in recent years (Tiberti et al. 2021).Furthermore, this is a sensitive issue as L. ghigii is a designated IUCN Vulnerable species, for which a published national action plan for its conservation exists (Spala 2021), so a careful approach to planning management actions and communicating the problem is required.If an eradication campaign or a containment operation is instigated, animal welfare issues must be taken into careful consideration (Crowley et al. 2017).To reduce any potential conflict, a careful study of the issue must be undertaken (Warren 2021).We propose a special feasibility study be developed to plan all aspects of the management and potential eradication and/or containment actions including the public communication requirements.

Figure 1 .
Figure 1.Two small Leuciscidae species occurring in the vicinity of Rafina in Attika.Adult Pelasgus marathonicus from the Boeotian population, Kifissos River basin (photo courtesy of J. Freyhof) (top), and Ladigesocypris ghigii from the Rafina translocated population; a very large individual: 95 mm in total length (photo by D. Zogaris) (bottom).

Figure 2 .
Figure 2. The 50% majority rule consensus Bayesian phylogenetic reconstructions for both mitochondrial cytochrome b and nuclear RAG1 sequence data.Numbers on branches represent posterior probabilities; only values ≥ 0.7 are shown.Samples from the introduced population near Rafina are in red.

Figure 3 .
Figure 3. Haplotype networks of Ladigesocypris sequences constructed by a statistical parsimony method for both mitochondrial cytochrome b and nuclear RAG1.For RAG1, all four possible alleles of heterozygotic sample Legh4 are depicted.Arrows in the RAG1 network indicate haplotypes of Legh4 sample with the highest probability as estimated by phasing the dataset by DnaSP (probability 0.65).Intermediate non-occurring haplotypes between our haplotypes are shown as small white circles.A line between two neighbouring haplotypes indicates one mutational step.

Table II .
Morphometric data of Ladigesocypris ghigii found at two sites near Rafina (N = 10).SD is standard deviation.

Table IV .
Scoring output from the Aquatic Species Invasiveness Screening Kit for Ladigesocypris ghigii in the risk assessment area of Attika region, Greece.

Table III .
Frequency of occurrence of meristic characters of Ladigesocypris ghigii found at two sites near Rafina (N = 10).Counts of transverse scales and transverse scales at caudal peduncle do not include the scale row on dorsal and ventral midlines noted as " 1 / 2 " in the text.