The effect of planktivorous fish (juvenile Perca fluviatilis) on the taxonomic diversity of microplastic particles-colonized bacterial community

Abstract Very few studies have investigated the influence of fish on the degradation rate of microplastic particles (MPs). It could be expected that their effect might be not only direct, through digestion and passage through the gut, but also indirect, through the alteration of the density and composition of the bacterial community on the surface of MPs. This study aims to test the short-term effects of fish presence altering the water chemical parameters and the abundance and genetic diversity of the aquatic bacteria colonising MPs, which may contribute to their faster degradation. This was tested in a triplicate experiment, with each replicate lasting 10 days in the presence or absence of spherical MPs, polystyrene (Ps) or polyethylene (Pe) and the presence or absence of a perch. The animal was separated from the MPs with a plankton net. We determined the chemical parameters of the water, the number of bacteria in the water and on the MPs’ surface, the taxonomic diversity of bacteria in the water and those present on the MPs using 16S rRNA gene (16S rDNA) sequencing, and the alterations of the MPs’ surface using a scanning electron microscope. The exposure to fish increased the concentration of ammonium, nitrates, and orthophosphates and the number of bacteria in the water, which in turn resulted in an increase in the abundance of bacteria and the number of taxa at the family level on the MPs’ surface. The positive effect of fish exudates on the abundance of bacteria on MPs’ surface was greater in the case of Pe than in Ps. The observed effects did not affect the MPs’ surface in any of the types of plastic during the experiment, but they may play a significant role in MPs decomposition over a longer than applied time period.


Introduction
Since the invention of organic polymers in the early 1900s, annual production of those light, multipurpose, insulation, and cheap materials has consistently increased, reaching 368 million tons in 2019 (Plastics Europe, and EPRO 2021). It has long been known that plastic accumulates in the environment due to improper waste disposal practices (Kenyon & Kridler 1969). However, until 1972, the main focus was on larger plastic items. It was only E. J. Carpenter who noticed the microplastics contamination (Carpenter et al. 1972), although the term itself was coined in an article investigating traces of plastic fragments and fibres in marine habitats, entitled Lost at Sea: Where Is All the Plastic? (2004), which ignited the microplastics research boom.
Microplastics are any synthetic solid particles or polymeric matrices with a regular or irregular shape and a size ranging from 1 µm to 5 mm (Cole et al. et al. 2020) and nanoplastics (<1 µm; Klaine et al. 2011;Gigault et al. 2018;Maszczyk et al. 2022aMaszczyk et al. 2022b, of either primary or secondary manufacturing origin (purposely produced in microscopic sizes or degraded from larger plastic fragments) (Cole et al. 2011). Polypropylene, polyethylene, polystyrene, polyvinylchloride, and polyethylene terephthalate are the most commonly detected microplastic contaminants in environments around the globe (e.g. Ng & Obbard 2006;Hwang et al. 2020;Stanton et al. 2020). Microplastic particles (MPs) are commonly found in environments ranging from marine (Cole et al. 2011) and freshwater (Eerkes-Medrano et al. 2015), through soil ecosystems (Chae & An 2018), even in hard-to-reach places such as the Mariana Trench , Arctic and Antarctic (Bessa et al. 2019) or in the sediments of remote mountain lakes (Allen et al. 2019). MPs are also present in food items such as table salt (Danopoulos et al. 2020) and beer (Shruti et al. 2020) and were also found in animals (Browne et al. 2008), including human (Ragusa et al. 2021) tissues. The earliest traces of microplastics in living organisms were found in the digestive tissues of freshwater fish in a museum collection dating back to the 1950s (Hou et al. 2021). Due to the increasing worldwide production of plastic materials, the concentrations of MPs in the environment are increasing, and predictive models show that this trend will only accelerate in the coming years if appropriate mitigation measures are not taken (van Wijnen et al. 2019).
Currently, plastic pollution is receiving worldwide attention due to emerging discoveries regarding its impact on the environment and human health. The influence of microplastic pollution was confirmed at all integrative levels of biological organisation, including genetic (Sun et al. 2021), cellular (Prinz & Špela 2020), physiological (Franzellitti et al. 2019), organismal (Schrank et al. 2019), population (Amariei et al. 2021) and community (Lozano & Rillig 2020;Zebrowski et al. 2022). The effect may differ depending on particle degradability (biodegradable or non-biodegradable; Tang et al. 2021;Zebrowski et al. 2022), size and shape (Covernton et al. 2019), chemical properties , colour (Chen et al. 2020) and concentration (Sun et al. 2022). However, the reported effects in microplastic studies range from no observed toxicity to observations of a variety of toxicological endpoints (Booth & Sřrensen 2020). Furthermore, the concentrations used in the studies that found the effect usually far exceed those found in the natural environment (Lenz et al. 2016;Ockenden et al. 2021).
It has been demonstrated that various living organisms can affect the qualitative and quantitative (crushing MPs into a larger number of smaller fractions) characteristics of MPs. The presence of organisms can affect the circulation of MPs in the environment, including their removal from the water column by deposition in sediments (Pukos et al. 2023) and transfer within food webs (Elizalde-Velázque et al. 2020). Biotic degradation of plastics can occur directly: physically (by biting or chewing (So et al. 2022)) or chemically (enzymes during digestive degradation (Dawson et al. 2018;Cornwall 2021)).
Among the life forms examined in this context, microorganisms have received the most attention (Bahl et al. 2020;Matjašič et al. 2021;Venkatesh et al. 2021). Despite plastic's hydrophobicity, its possession of stable covalent bonds and its high molecular weight, it has been suggested that, due to evolutionary selective pressure, the more the environment is exposed to plastic pollution, the higher its plastic-degrading potential (Yoshida et al. 2016). Plastic degrading bacteria have been discovered in habitats ranging from the open ocean to insect (Danso et al. 2019) and clitellate (Huerta Lwanga et al. 2018) digestive tracts.
Microbial plastic degradation can be divided into two categories: direct (a microbe using plastic as a source of nutrients) or indirect, causing deterioration (alteration of physical and chemical traits) or degradation (fragmentation) of a polymer through exposure to a microorganism's metabolic products (Singh & Sharma 2008). Indirect biotic degradation might as well occur by organism-associated microbial activity (Widyananto et al. 2021). However, the indirect type of biotic degradation is the least studied. Studies on free-living and animal-associated bacterial plastic degradation are scarce; only one study confirmed the effect of coral-associated bacteria (Widyananto et al. 2021).
MPs are good substrates for microbial colonisation due to their low biodegradability, high surfacearea-to-volume ratio, and hydrophobicity (Murphy Fish presence versus bacteria on microplastics et al. 2020), because of this they provide new microbial niches in aquatic environments (Yang et al. 2020). The colonisation of microplastics is well documented and occurs within a few days (Harrison et al. 2014). Apart from outside factors, like temperature or water flow speed, microplastic characteristics, such as surface roughness (Kerr & Cowling 2003) and polymer type (Frère et al. 2018) influence the process of biofouling and the composition and structure of that bacterial community. It is well established that bacteria growing in biofilms are usually physiologically and taxonomically distinct from free-living bacteria. Biofilms are typically characterised by dense clusters of bacterial cells. These cells secrete extracellular polymeric substances that hold the cell aggregates together in a biofilm matrix (Hall-Stoodley et al. 2004).
In the natural environment, MPs come into contact with a variety of bacterial strains, including bacteria associated with freshwater fish, which have not been studied in-depth in the context of MPs' colonisation and degradation potential and their influence on the genetic diversity of the MPs' bacterial community. Fishes possess a diverse associated bacterial community (Wilson et al. 2007), which increases the possibility that some of these bacterial strains could potentially have a biochemical or physical impact on MPs.

Flavobacterium)
and Actinobacteriota (e.g. Corynebacterium and Micrococcus) are other dominant phyla, except in carnivorous fish (Cahill 1990;Li et al. 2014). Other dominant microbiota in the intestine of freshwater fish are represented by Fusobacteriota and Tenericutes (Kashinskaya et al. 2019). This general pattern was confirmed for the microbiota of the perch (Perca fluviatilis), which is dominated by the phyla Proteobacteria, Firmicutes and Fusobacteriota, as well as Tenericutes (Kashinskaya et al. 2019). As the composition of the microbial communities associated with the fish is dominated by the phyla in which bacterial taxa having the ability to break down MPs were identified most often (e.g. Proteobacteria, Firmicutes, Actinobacteriota, Bacteroidota, and Fusobacteriota), it is very likely that the fish presence may result in an increase in the contribution of those taxa on the surface of MPs, which in turn may result in an increase in the degradation rate of MPs. The composition of the microbiota of perch at the genus level varied between different studies, being dominated either by Myroides, Prochlorococcus, Anabaenopsis, Cetobacterium and Fusobacteriaceae (Zha et al., 2018) or Aeromonas spp. and Pseudomonas spp. (Goldschmidt-Clermont et al., 2008) or Escherichia spp., Bacillus spp., Aerobacter spp., and Proteus spp. (Horsley 1977).
So far, the bacteria used in biodegradation studies have primarily come from plastic-polluted environments or animal intestines (Matjašič et al. 2021), without taking into account the degradation potential of animal-associated free-living bacteria. Due to the fact that the presence of aquatic organisms, including fish, changes both the water chemistry (e.g. the content of nutrients and pH) and the taxonomic composition of microorganisms, it might affect the rate of MPs' degradation. These assumptions have yet to be experimentally validated. Therefore, the aim of our research was to test several hypotheses concerning the effect of fish presence on the bacterial community colonising MPs and in turn, their potential to increase the decomposition rate of MPs. The first: the presence of a fish affects the quantitative (density) and qualitative (taxonomy) characteristics of free-living bacteria and bacterial communities colonising the MPs' surface by changing water chemistry parameters and introducing new bacterial strains. The second: there is a notable difference in fish influence on bacterial communities attached to the surface of different types of MPs. The third: the presence of the fish indirectly (through alterations of the water chemistry and bacterial composition on MPs particles) influences the quality features of the MPs' surface, measured as the diameter and circumference of the particles.

Experimental animals
Juvenile European perch (Perca fluviatilis) were collected by seining in Lake Roś, located in the Masurian Lake District in Poland, a region where microplastic freshwater contamination has been confirmed (Kaliszewicz et al. 2020). Planktivorous fish are abundant in this postglacial, dimictic, eutrophic lake (Jachner 1991) that is also exposed to strong anthropogenic pressure. During the day, juvenile perch, which are planktivorous, migrate there to the littoral, where they prey mainly on copepods (Jachner 1991). All captured fish measured less than 7 cm in length. Before the experiment, the animals were kept for 6 days in an 416 M. Pyznar et al. aquarium with preconditioned tap water (homogenised and filtered through a 2 µm mesh filter), an air pump, and an aquarium heater, in order to acclimate them to the laboratory environment, and then randomly selected for appropriate variants. The experimental procedure was approved by the First Warsaw Local Ethics Committee for Animal Experimentation (Permit protocol No. 1350P1/ 2022).

Microplastics
In the experiments, we used two different types of synthetic, non-biodegradable polymers, with a density similar to that of natural water (Zhou et al. 2021), in the form of microspheres. The first one was polystyrene (Ps), slightly heavier than water, which causes its faster sedimentation (d = 1.05 g × cm −3 ), with a mean diameter of 258.3 µm, catalogue number PS250K (Lab261®, USA). The second type of plastic was a highdensity polyethylene (Pe), slightly lighter than water (d = 0.96 g × cm −3 ), which causes floating close to the surface, with a mean diameter of 275 µm, catalogue number 110616-4 (Cospheric®, UK). Those two polymers are among the materials most commonly identified as microplastic pollutants (Wu et al. 2016).

Experimental setup
The experimental system for fish was comprised of eighteen 5 L glass containers placed randomly in a water bath (L = 220, W = 40, H = 40 cm, V = 352 L) holding internal submersible waterheaters (Aquael® Neoheater 150 W) and water pumps (Aquael® Circulator 500), inside a room, which allowed to maintain stable temperature (21 ± 0.2°C) and light conditions. The light intensity reflected the summer photoperiod (16 L:8°D, 10.0 ± 0.5 µmol°×°m −2 × s −1 , just under the surface of each of the containers) measured with Li-Cor 189 quantum sensor measuring radiance (Li-Cor Biosciences®). The potential influence of external light sources was eliminated by wrapping the walls of the water bath with a black, opaque film. The water bath was filled with aerated tap water, which was conditioned 6 days before the experiments (filtered through 2 µm mesh filters). The experimental containers were connected to the 8-channel aeration pumps (Hailea® ACO-9630) via pipes ending with glass tubes in order to minimise the contact of experimental plastic with laboratory plastic. In each of the 18 containers, a 150 µm net attached to a U-shaped frame was placed, which separated MPs from the animal in order to avoid the possibility of potential MP degradation as a result of passage through the digestive system. The intentional placement of the net in control tanks was aimed at ensuring uniform experimental conditions. A concentration of 3000 MPs × L −1 was utilized to promote adequate surface area for bacterial proliferation. During the experiment, we minimised the contact of the experimental organisms with plastic objects other than those used as a factor in the experiment. For example, all containers, tubes, and frames that came into contact with water in each tank were made of glass.

Experimental procedure
The experiment was conducted in July 2021 at the Hydrobiological Station in Pilchy, University of Warsaw, thanks to the authorities of the station. Eighteen containers were the combination of six variants replicated simultaneously three times, during a 10-day experiment. Each day at 10 a.m., the fish were fed with frozen bloodworms, and temperature and oxygenation were measured using an oxygen probe (YSI ProODO). Any leftover food debris was immediately removed. After 10 days, we collected samples of water and MPs. We examined the variants with the presence or absence of a fish and the presence of two types of microplastics. The experiment included the following variants: a fish and Ps particles (F-Ps), a fish and Pe particles (F-Pe), Pe particles only (Pe), Ps particles only (Ps), fish only (F) and water only (C) as a control.
A randomly selected perch was placed in each container with a fish variant (F, F-Pe and F-Ps). The experimental conditions, i.e. the size and structure, the amount of water, light exposure, temperature, and oxygen supply, were the same for each glass container, representing a single replicate of one of the six variants.
At the end of the experiment, in order to examine changes in water chemistry caused by the presence of a fish or possibly by the presence of MPs, concentrations of ammonium nitrogen, nitrates, and orthophosphates were measured in each of the 18 glass containers. For the analysis of ammonium ions, we used a method proposed by Holmes et al. 1999. For the analysis of total nitrogen, we used commercially certified Merck-Millipore cell tests (Spectroquant® nitrogen (Total) Cell Test 1.14537) according to the manufacturer's instructions, using the Merck Spectroquant Pharo 300 spectrophotometer. The orthophosphate concentrations were determined using the molybdenum method (Nagul et al. 2015) using a Shimadzu UV-VIS 1201 spectrophotometer.

Fish presence versus bacteria on microplastics
For the calculation of bacteria abundance, samples of MPs and water were conserved with 2% formalin and processed according to Porter and Feig (1980). Free-living bacteria and bacteria attached to MPs were stained with DAPI (4',6-diamidino-2-phenylindole, 10 min, temp. 24°C, final DAPI concentration -1 µg L −1 ), suspended on the surface of 0.2-µm, black polycarbonate membrane filters (Millipore) by filtration and then examined under Nikon epifluorescence E450 microscope equipped with Nikon Digital Camera DXM 1200 F and NIS elements software (Nikon). In water samples, free-living bacteria were counted in 10 randomly selected 10 fields of view. In MPs' samples, biofilm bacteria were counted on the surface of 10 randomly selected particles and expressed as the number of bacteria per µm 2 .
For taxonomic identification of bacteria on MPs nearly 2000 particles were collected through a sterilised strainer and immediately frozen for further DNA isolation. For taxonomic identification of free-living bacteria 200 ml of water from the experimental tank was filtered through the 0.2-µm polycarbonate filter (Merck Isopore™ Membrane Filters), and the filter was also placed in the Eppendorf probe and frozen. The filters were cut into small pieces prior to DNA isolation. DNA extraction was performed using the Gene MATRIX Soil DNA Purification Kit (EURx, Poland) according to the manufacturer's instructions.
In order to examine the surface and determine any potentially visible surface alterations caused by the bacteria and biofilm presence, we used scanning electron microscopy (SEM). The images of the particles were obtained in the Laboratory of Electron and Confocal Microscopy at the Faculty of Biology, University of Warsaw. The dried microplastic particles were mounted on a SEM mounting stub, sputter-coated with gold using the POLARON SC7620 metal sputtering machine by Microtech, and examined with the use of a LEO 1430VP ZEISS scanning electron microscope with 400 × magnification.
For the ratio of the changed surface to unchanged, for the number of visible bacteria on the surface and for the presence or absence of biofilm on SEM photographs, we used ImageJ 1.53 g software (Schindelin et al. 2012).

Data analyses
The statistical analysis was performed using the R platform (v.4.2.0). In all statistical tests, the significance level was set to α = 0.05. Before selecting an appropriate test, we checked the assumption for ANOVA (analysis of variance) by performing the Shapiro-Wilk normality test and Levene's test for homogeneity of variance.
To test the differences in the abiotic parameters (temperature and oxygen concentration) between all the experimental treatments, we performed a threeway mixed ANOVA using the "ezANOVA" function (the "ez" package v.4.4-0). The Greenhouse-Geisser correction was applied if the sphericity checked by Mauchly's test was violated. The presence of the fish and MPs (Ps or Pe) were set as between-subject factors, and time as a within-subject factor. To test the effects of the presence of a fish and MPs (Ps or Pe) on the number of bacteria in water, the chemical parameters of water quality (the concentration of ammonium nitrogen, nitrates and orthophosphates) and the effect of the presence of a fish on the number of bacteria on different types of MPs and the microplastics' physical properties (diameter and circumference), we conducted a twoway ANOVA using the "aov_car" function ("afex" package v.1.1-1).
To determine the taxonomic composition of the bacterial community, Bray-Curtis-based NMDS (nonparametric multidimensional scaling) was performed on relative abundance of bacteria domain representatives on the family level.
Planned contrasts for estimated marginal means (EMMs; the "emmeans" package v.1.7.5) were used to compare the effect of fish with control separately for each of the types of microplastics on the chemical parameters of water quality, on the number of free-living bacteria, on the number of bacteria on MPs and on MPs' physical properties. The "holm" p-value adjustment was applied to control Type I error inflation as a result of multiple testing.

Microplastics physical properties -diameter and circumference
The presence of fish did not affect the diameter and circumference of MPs at the end of experiments (Table I, Figure 1(a, b)); however, the circumference depended on the type of MPs (Table I, Figure 1(a, b)). The diameter and circumference of Pe (in relation to Ps) were greater in both the presence and absence of fish (Table S1, Supplementary materials, Figure 1(a, b)).

Experimental conditions
Oxygen concentration was affected by the presence of the fish (Table II). The effect was apparent in 418 M. Pyznar et al. both the absence of MPs and in the presence of Pe (Figure 2). The temperature value and oxygen concentration were not affected by the presence of MPs (Table II, Figure 2).

Chemical parameters of water quality
Generally, the presence of a fish had a significant effect on the concentrations of orthophosphates and ammonium ions, however there was no significant effect of fish on the total nitrogen concentration ( Table II). The presence of MPs did not affect any of the chemical parameters of water (Table II). The concentration of orthophosphates was greater in the presence of fish than in its absence in the treatments with MPs (Table S2, Figure 2). The concentration of orthophosphates was also significantly higher in the presence of the fish and Pe, compared to the presence of the fish without Pe (Table S2, Figure 2). In the presence of the fish, the concentration of orthophosphates was significantly higher in the treatment with Pe than in the treatment with Ps (Table S2, Figure 2). The ammonia concentration was higher in the presence of the fish (both with and without MPs, independently of their type) compared to the control (Table S2, Figure 2), and was greater in the presence of fish than in its absence in both types of MPs (Table S2, Figure 2). Moreover, the concentration of ammonia was significantly higher in the presence of the fish and Ps, compared to the presence of the fish without Ps (Table S2, Figure 2). The total nitrogen concentration was significantly higher in the treatment with fish and Ps compared to the treatment with Ps without fish (Table S2, Figure 2).

Number of bacteria
The number of free-living bacteria was affected by the fish presence and by the type of MPs (Table III, Figure 3(a)). In all variants with the presence of the Table I. The results of two-way ANOVA to test the effect of the presence of the fish (F) and MPs type on MPs' physical properties (diameter and circumference) at the end of experiments. Statistically significant (α = 0.05) differences are marked with bold (df -degrees of freedom. SS -Sum of Squares. F -F-statistics, P -p-value).

Parameter
Factor; Interaction df SS F P

Fish presence versus bacteria on microplastics
fish (with and without each of the types of MPs) the number of bacteria was greater compared to the control (Table S3, Figure 3(a)). In the Pe treatments, the number of bacteria was greater in the presence than in the absence of fish (Table S3, Figure 3(a)).
In the case of the number of bacteria on the MPs' surface, we observed both the effect of the fish presence and the type of MPs (Table III, Figure 3(b)). The number of bacteria on the MPs' surface was greater in the presence than in the absence of fish and at the surface of Ps than Pe particles (Table. S3, Figure 3(b)). The analysis of the planned contrasts revealed that the effect of the fish was significant in each of the plastic types, and the difference between MP types was significant in both the presence and absence of fish (Table S3, Figure 3(b)).

Taxonomic composition of bacterial community
The most abundant bacterial taxa at the phylum level in all samples were Proteobacteria, followed by Bacteroidota and Verrucomicrobiota. In freeliving bacterial communities, greater contribution of Verrucomicrobiota and Actinobacteriota was apparent in the fish than in no-fish media. The most apparent difference between free-living bacteria and bacteria attached to MPs particles was the lower contribution of Bacteroidota and Proteobacteria and the greater contribution of Bdellovibrionota in the latter than in the former. Moreover, Actinobacteriota were present in water samples in the fish treatments but not on the surface of particles, neither in fish nor in no-fish treatments. Also, Cyanobacteria was present only in free-living bacteria fraction in several treatments (Pe, F-Pe and Ps), but not on the surface of MPs (Figure 4(a, b)).
On the MPs' surface, greater contributions of Verrucomicrobiota and Bacteroidota and a lower contribution of Proteobacteria were in the fish medium in relation to the no fish medium. On the surface of Ps particles in the fish medium, the significant phylum was Fusobacteriota, and on the surface of Pe particles in the fish medium, the significant phyla were Myxococcota and Acidobacteriota (Figure 4).
The number of taxa at the family level of the freeliving bacteria was lower in the fish medium, and greater in the presence of MPs (in both fish and no fish medium) in relation to the control (Figure 5(a)). The number of taxa at the family level of bacteria on MPs' surfaces was greater in the presence than in the absence of fish in both types of MPs ( Figure 5  (b)). In the absence of fish, the number of taxa on MPs was lower compared to the number of taxa of free-living bacteria. In the presence of fish, this relation was reversed. Table II. Results of the three-way mixed ANOVA to test the effect of all experimental treatments (F -presence of the fish, type of MPs and Ti -time) on the abiotic parameters (temperature and oxygen concentrations) and the results of the two-way ANOVA to test the effect of the presence of the fish and type of MPs on the chemical parameters of water quality (the concentrations of orthophosphates, ammonium ions, and total nitrogen). Statistically significant (α = 0.05) differences are marked with bold (df -degrees of freedom, F -F-statistics, P -p-value).

Fish presence versus bacteria on microplastics
The Simpson 1-D index for the free-living bacteria was relatively higher in the presence of fish. The effect of MPs was only apparent in the case of Ps (Figure 5 (c)). The Simpson 1-D index for samples collected from MPs' surface was significantly greater in the presence of fish compared to its absence for both types of MPs ( Figure 5(d)). In the absence of fish, the Simpson 1-D index for the taxa on MPs was lower compared to its value for free-living bacteria. In the presence of fish, this relationship was reversed.
The NMDS of the values of the Bray-Curtis index calculated on the basis of the relative Table III. The results of a two-way ANOVA to test the effect of the presence of the fish (F) and microplastics particles (MPs) on the number of free-living bacteria in the experimental media and on the number of bacteria on MPs at the end of the experiment. Statistically significant differences (α = 0.05) are marked with bold (df -degrees of freedom, SS -Sum of Squares, F -F-statistics, P -p-value).

Parameter
Factor; Interaction df SS F P    (Figure 6). In general, at the family level the taxonomic composition of the biofilm bacteria community is different from that of the free-living bacteria community in the context of different proportions of the same bacterial families on the particles and in water ( Figure 6).

Discussion
The results of our study confirmed the first hypothesis, as the presence of a fish affects the quantitative (density) and qualitative (taxonomy) characteristics of free-living bacteria and bacterial communities colonising the MPs' surface. The effects were accompanied by a large increase in the concentrations of orthophosphates and ammonium ions and the nitrogen concentration was higher in the treatment with fish and Ps. The effect of the fish on the increase in concentrations of dissolved mineral phosphorus and nitrogen is consistent with the numerous theoretical (e.g. Zimmer et al. 2006;Verant et al. 2007) and experimental (Kitchell et al. 1975;Threlkeld 1987;Vanni & Findlay 1990) studies on the effect of fish egestion and excretion on water chemistry, which revealed that it can be an important source of nutrients in aquatic ecosystems (e.g. Atkinson et al. 2017). The potential for significant contribution of a fish to phosphorus recycling in limnetic systems via excretion was first raised by Kitchell et al. (1975), who concluded that phosphorus excretion by bluegill (Lepomis macrochirus) in a small lake may support algal populations during periods of low phosphorus availability. In other studies, it has been revealed that nutrient release by fish might play an important role in phosphorus cycling and algal production (Threlkeld 1987) and that excretion of phosphorus by fish can be equal to or even greater than that of zooplankton (e.g. Vanni & Findlay 1990;Carpenter et al., 1992). It has been revealed that fish activity may increase the abundance of free-living bacteria directly through released nutrients and indirectly through Fish presence versus bacteria on microplastics trophic interactions (e.g. Jeppesen et al. 1998;Villéger et al. 2019), although most of the previous studies did not disentangle these effects. For instance, in the long-term study of shallow and hypertrophic Lake Sřbygĺrd (Denmark), it has been revealed that temporal changes in fish stock affected through cascading effects the abundance of bacterial communities, although this effect was less apparent than the cascading effect on zooplankton and phytoplankton (Jeppesen et al. 1998). In other study, it has been revealed that the Mediterranean fish -Sparus aurata had a positive effect of nutrient excretion on the abundance of the microbial plankton, including bacterioplankton in the absence of large zooplankton when their growth was limited by nutrient availability (Villéger et al. 2019). As the dominant organisms in our experimental media were bacteria, it could be assumed that the increase in their abundance was only due to direct effect i.e. due to nutrients released by the fish. We are aware of only a single previous study in which a similar observation was made (Maszczyk & Bartosiewicz 2012). In this study, chemicals originating from fish activity fertilised the environment enough for a significant increase in bacterial abundance, which positively affected the growth and other life history parameters of their consumer -the planktonic cladoceran Daphnia. More importantly, in our study the greater abundance of the free-living bacteria was parallel to a greater abundance of bacteria on the MPs surface. To our knowledge, this is the first evidence in the literature concerning this issue. As only some bacterial strains possess the ability to decompose plastics, an increased abundance of bacteria in the presence of fish exudates increases the chance of more efficient MPs decomposition.
The results of our study also revealed that the nutrient excretion by fish altered the taxonomic composition of the free-living bacteria, which is consistent with numerous previous studies (e.g. Schaus & Vanni 2000;Villéger et al. 2019;Molina & Fernández 2020). For instance, it has been revealed that the nutrient excretion by the Mediterranean fish -Sparus aurata altered the community structure of the microbial plankton, including heterotrophic bacteria in the absence of large zooplankton (Villéger et al. 2019). A similar effect was observed by Schaus and Vanni (2000), who found that the gizzard shad (Dorosoma cepedianum) excretion resulted in changes in the relative abundance of Figure 6. NMDS of the values of the Bray-Curtis index calculated on the basis of relative abundances of taxa on family level in water and on the microplastic particle surface at the end of the experiments (mean ± 1SD) in all of the treatments, separately in each of the two experiments for which the analysis was performed (a and b in the subscript).

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M. Pyznar et al. different taxa within the phototrophic unicellular organisms, including Cyanobacteria. Although the aforementioned studies indicated that the presence of fish exudates differentiates bacterial composition, they did not disentangle the direct effect of nutrients released by the fish or the indirect effect of the metabolites excreted by other organisms present in the experiments. Another example of the study concerned the effect of dissolved organic matter and inorganic nitrogen release through fish (Salmo salar) epithelium mucus on the natural bacterial assemblages from Northern Patagonia, Chile (Molina & Fernández 2020). The study revealed that fish mucus can cause rapid modifications in microbial assemblages, which was apparent in the relative increase in the abundance of Proteobacteria and Bacteroidota. The results of our study are not consistent with this observation, as we did not find any difference in the relative contribution of Proteobacteria and Bacteroidota between the fish and no fish media. Instead, Verrucomicrobiota and Actinobacteriota were more numerous in the fish media. Moreover, Actinobacteriota was specific only to the fish media. Overall, the number of taxa at the family level of the free-living bacteria was lower, but their diversity was greater in the fish medium. It could be assumed that the change in bacterial composition in the fish media was due to the net effect of altered physicochemical water parameters, including nutrients and oxygen concentrations, and enrichment of the media by introducing fish-associated bacterial strains. As different bacterial strains have different environmental requirements, it could be expected that altered environmental parameters due to the presence of fish stimulate changes in interactions within and between different bacterial taxa, having a profound impact on the outcome of their competition (e.g. Hibbing et al. 2009). For the same reasons, decreased oxygen concentrations in fish treatments, which were caused by the fish's respiration, might have influenced the composition of free-living bacteria as well. Overall, the changes in microbial composition obtained in our study in the fish medium are consistent with the broad literature concerning the composition of fish-associated bacteria, in which Proteobacteria is the dominant phylum in all fish species, and Actinobacteriota are also well represented (Cahill 1990;Li et al. 2014). In our study, the change in the taxonomic composition of free-living bacteria was accompanied by a change in the taxonomic composition of bacterial assemblages on the surface of the MPs. Overall, in the bacterial assemblages on MPs particles, there was a lower contribution of Bacteroidota, Proteobacteria, Actinobacteriota, and Cyanobacteria and a greater contribution of Bdellovibrionota, Fusobacteriota, Myxococcota, Acidobacteriota, and Verrucomicrobiota than in free-living bacteria fraction. The results are consistent with the numerous studies revealing that biofilm communities on MPs differ from the communities in the surrounding environment (e.g. Rummel et al. 2017;Ogonowski et al. 2018;Miao et al. 2019;Rosato et al. 2020). Therefore, MPs can be considered a distinct ecological habitat for diverse microbial communities, the "plastisphere" (Zettler et al. 2013), potentially characterised by distinct microbial and ecological functions (Miao et al. 2019). Moreover, our study seems to be the first one concerning the alteration of the composition of bacterial assemblages on the MPs surface in the presence of fish. It is clear from the study that the number of taxa and their variability in the bacterial assemblages on the MPs surface increased in the presence of fish. This was evident in the increase in the relative contributions of Fusobacteriota, Myxococcota, Acidobacteriota, Bacteroidota and Verrucomicrobiota, which was accompanied by a decrease in the relative contribution of Proteobacteria on the MPs surface in the fish media in relation to no fish media, which suggests that the presence of the fish introduces new bacterial strains with a greater potential to create biofilms. Dominant bacterial strains in the absence of fish are potentially being displaced competitively by fishassociated bacteria due to the competition for surface. Due to the fact that only some strains possess the ability to decompose MPs, it is not possible to state whether the fish exudates modify the rate of MP decomposition based on how it affects the relative contribution of different taxa at the phylum level. However, as the composition of the microbial communities on MPs surface associated with fish presence is more diverse, it can be assumed that the chance that such a community contains bacteria with the ability to decompose MPs increases.
The results also confirmed predictions of the second hypothesis as we observed apparent differences in the fish effect on bacterial communities associated with the surface of different types of MPs. The positive effect of fish was greater on the abundance of the bacterial community on the surface of Pe than on Ps. Although our study seems to be the first one concerning the effect of fish exudates on the bacterial communities associated with the surface of different types of MPs, the results of our study are Fish presence versus bacteria on microplastics consistent with previous observations that the microbial community on MPs' surface depends on the polymer type (e.g. Hori et al., 2010;Fotopoulou et al., 2012;Hossain et al. 2019;Parrish & Fahrenfeld 2019;Rosato et al. 2020;Tu et al. 2021). For instance, it has been revealed that within 2 weeks of incubation, the bacterial abundance was greatest on PVC, lower on Pe, and lowest on PET, Pp, and Ps pellets (Rosato et al. 2020). Also, Hossain et al. (2019) showed that bacterial colonisation of MPs is affected by the physicochemical properties of MPs, including their surface roughness and the type of plastic, although they did not find a difference in the bacterial density at the surface of the types of plastics used in our study (HDPE and Ps). However, it should be pointed out that in our study, the effect of fish was similar in terms of the number of taxa and diversity of taxonomic composition on the surface of both types of MPs. The only differences were apparently greater effects of fish on the contribution of Fusobacteriota on the surface of Ps and a greater effect of fish on the contribution of Myxococcota and Acidobacteriota on the surface of Pe. This is visible also on the SEM photographs (Figure 1(b)), which indicate that Ps has a relatively smooth surface in comparison with Pe with a more granular structure on the surface and with that some Fusobacteriota have strong abilities to attach to smoother surfaces (Moses et al. 2020).
Additionally, the presence of different MPs also had a slight but significant effect on water chemistry, and this effect was different in the fish and no-fish media. That is, the presence of Pe results in an increase in the concentrations of orthophosphates and the presence of Ps results in an increase in the concentrations of ammonia only in fish treatments. This is in accordance with studies indicating that the presence of polystyrene increases ammonia excretion of black rockfish (Sebastes schlegelii; Yin et al. 2019) or disturbs it in medaka (Oryzias melastigma) gills (Zheng & Wang 2022). Another explanation may consist of the differential effect of MPs on the qualitative and quantitative compositions of freeliving and associated with MPs bacterial assemblages (e.g. Rosato et al. 2020), which may utilise nutrients produced by fish to a different extent.
The last hypothesis, that the presence of the fish indirectly (i.e., through alterations of the water chemistry and bacterial composition on MPs particles) influences the quality features of the MPs' surface, was not confirmed, as after 10 days, there was no significant change in the circumference and diameter of the particles of any type. However, the changes in the physicochemical parameters of the water as well as the quantitative and qualitative changes in bacteria in the presence of the fish exudates observed in our study suggest that a longer exposure to the fish exudates could accelerate the deterioration or decomposition of the MPs.

Conclusions
Overall, the results revealed that the presence of a fish affected the chemical parameters of the water and the quantitative (density) and qualitative (taxonomy) characteristics of free-living bacteria and bacterial communities colonising the MPs' surface. Although those changes did not affect the apparent modification of the surface of MPs, they suggest that longer than applied in our study, exposure to fish may have an effect on the decomposition of MPs. To the best of our knowledge, these results provide the first evidence of the direct effect of aquatic animals on the plastisphere and, in turn, their significance on the self-purification of water from plastics.