Eating in an extreme environment: diet of the European hare (Lepus europaeus) on Vesuvius

Abstract The European hare (Lepus europaeus) is cosmopolitan species, living in a variety of habitats and showing a diversified diet, that has been described mainly from agricultural meadows and crops, with little information available for extreme environments. Here, we describe, for the first time, the diet of the European hare from Mount Vesuvius, using DNA metabarcoding and high-throughput sequencing on DNA extracted from faecal pellets, a proxy for a population living in a volcanic environment. The DNA from pellets was first genetically assigned to European hare using high-resolution melting analysis. The diet of the hare on Vesuvius is mainly composed of herbaceous species belonging to Fabaceae (86.26% of total diet). The most frequent plant items ingested by the species are Galega officinalis and Lupinus angustifolius (67.10% of total diet), although these are detected only sporadically in the study area. Indeed, the spectrum of available plants also includes other easily accessible wild (i.e. Lolium sp., Bromus sp., Rumex sp.) and cultivated (i.e. Solanum lycopersicum, Cucumis melo, Pisum sativum) plant items, found only in traces in the diet of the hares. Our contribution adds information on the trophic ecology of the European hare, exploring its ability to live in an extreme environment. This could be useful to set a management strategy for conservation of the species, which is ecologically relevant on Vesuvius as prey for birds and mammals, as well as a vegetation modulator via selective grazing by endozoochory. Furthermore, our study represents the latest information on the diet of the hare living in an environment that no longer exists: an extensive fire destroyed about 80% of the woody area after our sampling. The post-fire regrowth is transforming the original environment and consequently the trophic availability for the European hare. Key policy highlights Conservation of biodiversity in a national park Ecosystem service of the European hare Management of a Lagomorpha game species


Introduction
The European hare (Lepus europaeus), also named the brown hare, is a cosmopolitan species, whose current distribution includes all regions from the northern Spain to northern portions of the Middle East to the Kazakhstan and Middle-Asian steppes, and areas of introduction as a game species in Argentina, Australia, Canada, New Zealand, southern Scandinavia, the United Kingdom, the United States and some islands, where the species has subsequently become established (Hacklander & Schai-Braun 2019).
The European populations have undergone a strong decline in many areas across the hare's geographic range since the 1960s, mainly blamed on the agricultural intensification and mechanisation (Smith 2008;Chagnon et al. 2015) that led to dramatic landscape changes and the reduction of habitat diversity (Jennings et al. 2006;Reichlin et al. 2006;Pépin & Angibault 2007;Storkey et al. 2012). Furthermore, other factors, such as infective diseases (Tsokana et al. 2020) and the climate changes, could have magnified Currently, the conservation status of the European hare worldwide is Least Concern; however, population declines have resulted in its Red Listing as Near Threatened or Threatened (Hacklander & Schai-Braun 2019) in some countries, such as Norway, Germany, Austria and Switzerland. To face population decline, restocking operations with captive-bred individuals as well as the translocation of wild hares among countries have been performed, in some cases determining potential risk consequences for native populations (Caravaggi et al. 2015).
In Italy, the species was originally distributed throughout the north-central regions in the form of the native subspecies L. europaeus meridiei (Pierpaoli et al. 1999;Kasapidis et al. 2005;Stamatis et al. 2009), isolated during the last glaciation. However, since the beginning of the 20th century, the massive and protracted restocking with allochthonous individuals (mainly from east Europe) (Pierpaoli et al. 1999(Pierpaoli et al. , 2003Kasapidis et al. 2005;Fulgione et al. 2009) has heavily altered this distribution, causing the replacement of many local indigenous populations (Pierpaoli et al. 1999(Pierpaoli et al. , 2003Freschi et al. 2015Freschi et al. , 2022 and leading to a spreading of the hare also into southern Italian regions. As a consequence, nowadays, the European hare is present across the whole Italian peninsula (except in Sicily and Sardinia), as a mix of native and exotic lineages (Angelici & Luiselli 2001;Kasapidis et al. 2005;Fulgione et al. 2009).
The European hare is one of the most managed mammals in Europe, mainly as game species. This raises problems related to its ability to hybridise and compete ecologically when in sympatry with other vulnerable hare species, such as the mountain hare (Lepus timidus) (Thulin 2003) and the Italian hare (Lepus corsicanus) (Angelici & Luiselli 2001;Mengoni et al. 2015;Pietri 2015;Buglione et al. 2020a). Furthermore, the European hare acts as a host of dangerous ticks, sandflies and other disease vectors (Ebani et al. 2016;Tsokana et al. 2020), spreading the related pathologies. This happens in the case of translocation of infected animals or when they serve as prey to the long-distance carnivores and omnivores travellers, coming into contact with humans and domestic animals (Bártová & Sedlak 2012). In terms of both conservation and management needs, it is necessary to know in depth the ecological requirements of the species in all relevant environmental contexts, to plan useful actions to meet these needs.
The European hare lives in a variety of habitats, from the arctic tundra and steppe to tropical savanna and desert, as well as in agricultural meadows, crops, and unimproved grassland; it avoids extended woodlands (Smith et al. 2005;Trocchi & Riga 2005;Smith 2008;Cardarelli et al. 2011;Sliwinski et al. 2019;Buglione et al. 2020a).
Accordingly, the species has a very diversified and variable diet, selecting mainly cultivated crops, but not disdaining weeds, wild grasses and forbs (Chapuis 1990). This flexible spectrum has only been partially described, and mainly for agricultural landscapes (Reichlin et al. 2006;Puig et al. 2007Puig et al. , 2017Schai-Braun et al. 2015;Castellaro et al. 2021); indeed, little information is available about the species' adaptation to a volcanic environment, with soil partially covered by lava and stratified pyroclastic deposits, affecting vegetation and plant formations.
Thus, we characterised the diet of the European hare by DNA metabarcoding and high throughput sequencing (HTS) starting from excremental DNA (Reichlin et al. 2006;Buglione et al. 2018;Hacklander & Schai-Braun 2019), as a proxy of the food habits in extreme environmental conditions, in particular on Mount Vesuvius (South Italy). This Italian volcano represents an interesting study area because it is home to a population of introduced European hare that has no way of dispersing outside the volcanic cone. Indeed, a concentric enclosure of the crater, consisting of very compact urban area, completely isolates the prairies and forests growing on the volcanic soils, and the hares living there. Thus, our study could add a new puzzle piece to deepen the knowledge of the trophic ecology of a isolated volcanic population.
Furthermore, immediately after our data collection, there was an extensive fire that impacted about 80% of the woody area (according to the Vesuvius National Park data base). The post-fire spontaneous regrowth and planting carried out by the national park are transforming the original landscape and consequently the food availability for the European hare. Considering this, our study could represent the latest information on the diet of the hare population adapted to Vesuvian habitats in an environmental context that no longer exists.

Study area
Vesuvius National Park (VNP) is a protected area that was established in 1995 (National Law no. 394/ 1991)  It represents a volcanic system including Mount Somma (1132 m above sea level) and Mount Vesuvius (1277 m above sea level), the latter being a stratovolcano (De Natale et al. 2006) with a typical truncated cone shape, a crater with a diameter of 450 m and a depth of 300 m (as of 2022).
The area is characterised by a Mediterranean climate with hot and dry summers and mild winters, sometimes covered with snow at higher altitudes. The mean annual precipitation is 1100 mm, the average minimum temperature of the coldest month (January) is 5.7°C and the average maximum temperature of the warmest month (July) is 21.6°C (Ducci & Tranfaglia 2008).
The Vesuvian landscape is characterised by different land uses, such as volcanic geo-sites, mixed broadleaved and coniferous forests, shrubland, farmlands, and high-density urban areas. The plant population of the volcano is also characterised by a high number of species typical of the Mediterranean area, constituting more than 42% of the entire flora. Among them, 53.5% were from Eurimediterranean areas, 42.1% were from Stenomediterranean regions and only 4.2% are typical of the Mountain Mediterranean sites. The number of plant species from the European and Asian continents is not negligible, differently from the species of Nordic (Boreal) and Western European (Atlantic) regions which, as a whole, do not reach 10% of the entire flora. Other quite numerous plant species are cosmopolitan and exotic, both cultivated and adventitious, explained by the high incidence of a strong anthropic impact.
On Mount Vesuvius, rocky lava flows are covered by the pioneer communities of endemic Vesuvius lichen (Stereocaulon vesuvianum) and typical spontaneous Mediterranean species such as Quercus ilex and Quercus pubescens, Cytisus scoparius and Spartium junceum, as well as non-native pine plantings, including stone pine (Pinus pinea), the most abundant planted species, and other Mediterranean coniferous species (Pinus pinaster, Pinus halepensis and Pinus nigra) (Di Gennaro et al. 2002;Ricciardi et al. 2016).
The volcano is surrounded by a vast urbanised matrix, with 13 municipalities and 351,018 residents (as of 2022; http://www.comuni-italiani.it/ parco/vesuvio/) falling within VNP and five other municipalities that, considering spatial contiguity and homogeneity of characteristics, can be considered an integral part of the context. In this scenario, the agricultural environment covering the slopes of Vesuvius represents a buffer zone between natural and urban areas, making the national park an important safety zone for both wild animal and plant species.

Study plot
In detail, we have selected an area of 7.74 km 2 ranging from about 500 to 1000 m above sea level, on the south-western side of the volcano, in order to avoid both soil covered exclusively with lava and pyroclastic deposits (up to 1000 m a.s.l), and intensive arable land due to its strong anthropisation ( Figure 1). The study area is characterised by lava layers that were deposited from 1798 to 1944 after repeated eruptions and solidification of flows of lava (Santacroce 1987). This ropy lava is covered by spontaneous Mediterranean vegetation with the prevalence of various species of broom (Genista tinctoria, Cytisus scoparius), shrubs, untilled farmland, mixed artificial pine forests and holm oak woods (Davoli et al. 2001). The undergrowth is typically rich in herbaceous and shrub species (Centranthus ruber, Festuca sp., Rosa canina, Rumex bucephalophorus, Ruscus aculeatus), and various species of wild orchids.
Although faecal pellets can be collected throughout whole National Park, we observed a higher likelihood of finding them in the study plot.
In addition to hare, some observations from rangers and technical documents report the presence of the wild rabbit (Oryctolagus cuniculus). The population of this lagomorph reached a peak of over 10,000 individuals in 2008 but is currently still considerably reduced (Accardo et al. 2008). Its distribution is focused mainly in plantations and arable lands at lower altitude of the southern slope, partially overlapping the European hare's range (Accardo et al. 2008). However, further in-depth studies are needed to clarify this aspect and the potential ecological relationship between these two lagomorphs.

Diet of Lepus europaeus on Vesuvius
we learned from our preliminary study by analysing signs of predation and video trapping data, the presence of stray dogs and cats should not be overlooked -in particular, the predation pressure by felines on the young hare (unpublished data).

Collection of samples
The surveys were performed monthly in spring-summer 2016 (from March to July) in order to include the greatest diversity of plant items available for the hares.
Fresh faecal pellets (<1 day old) were collected by field operators walking along a transect of approximately 5 km. The discrimination of specimens was made by using visual searching and selecing on the basis of aspect patterns (e.g. morphology, moist shiny mucous coating, colour, sheen, etc.). To increase the chance of collecting only fresh material, the sampling site was inspected the evening before collection and any faecal pellet found was removed. Furthermore, in a sampling session we collected pellets 20 m apart from each other, to minimise the possibility of recording the same individual multiple times (Buglione et al. 2018(Buglione et al. , 2020b. The samples, handled with sterilised equipment, were preserved dried in sterile tubes with silica desiccant granule bags, transported at a controlled temperature to the laboratory and preserved at −20°C until processing.
All records were geo-referenced using a global positioning system.

Species discrimination
All experimental procedures were performed in a laboratory designed for molecular analyses on environmental samples, with different rooms and separate equipment for pre-and post-Polymerase chain reaction (PCR) analyses.
DNA extraction for species characterisation. For species determination, hare DNA was isolated from the outermost layer of each scat, sliced off with a scalpel. These surface slices were used to extract DNA using the QIAamp DNA Fast Stool Mini Kit (QIAGEN GmbH Valencia, CA, USA), that specifically includes InhibitEx Buffer specifically aimed at removing PCR inhibitors. Together with the samples, we systematically performed blank extractions without samples, to monitor for potential crosscontaminations. The quantity of total isolated DNA was measured using a Thermo-Scientific (Nanodrop 2000) Spectrometer (Nanodrop, Wilmington, DE, USA) while the DNA integrity was tested by 1% agarose gel electrophoresis.
High-resolution melting analysis. Taking into account the sympatry between the Italian hare and European hare in Campania (Pierpaoli et al. 1999;Fulgione et al. 2009;Buglione et al. 2020a) and the impossibility of discriminating the two species using faecal pellet morphology, we characterised the faecal DNAs with high-resolution melting (HRM) analysis (Farrar & Wittwer 2017), according to the protocol reported by Buglione et al. (2020b).
Specifically, we performed HRM reactions on a Rotor-Gene Q 5-Plex (QIAGEN GmbH Valencia, CA, USA) with the Type-it HRM PCR Kit (QIAGEN GmbH Valencia, CA, USA), using 25-50 ng of DNA and 10 uM of each primer (Lep_F: 5'-TCAG CACCCAAAGCTGAAATTCTC-3'; Reverse_LE:5'-TGGGGAAGAGCTTTAATGCACGAT −3'; Rev erse_LC: 5'-CGTCAATAGTGACAAGGTACTTG GA-3' (Buglione et al. 2020b)). Cycling conditions were set up at an initial step for the HotStarTaq Plus DNA Polymerase activation for 5 min at 95°C, followed by 35 cycles of denaturation for 10 sec at 95°C, annealing for 30 sec at 62°C and extension for 10 sec at 72°C, activating fluorescence data acquisition on the green channel. The final melting step ramped from 65 to 80°C, with 0.1°C increments and 2 sec at each temperature. All runs included DNA from known Italian hare and European hare, as positive controls, and pure water as negative control. Two PCR replicates were performed for each sample.
Raw data were analysed using Rotor-Gene Q software v. 2.1.0 (QIAGEN GmbH Valencia, CA, USA), and the assignment of species was made by comparing the sample melting profile and the positive controls' melting curves.

Diet characterisation
Isolation of DNA for diet analysis. The faecal pellets assigned to the European hare were processed for diet characterisation as reported by Buglione et al. (2018). In brief, the innermost portion of the scats, with greater probability of containing the DNA of the ingested material, was used for the extraction of plant DNA by the hexade-cyltrimethylammonium bromide (CTAB) method (Doyle & Doyle 1987). Specifically, approximately 20 mg of material was homogenised in phosphate-buffered saline (PBS); after removing the soluble phase by high-speed centrifugation, isolated particles were suspended in CTAB buffer (2% CTAB, 100 mM TrisHCl pH 8.0, 20 mM Ethylen Diamino Tetracetyc Acid (EDTA), 1.4 M NaCl) with 0.2% βmercaptoethanol and incubated at 70°C for 30 min. The DNA was isolated from lipids and proteins by mixing with chloroform/isoamyl alcohol (24:1) and centrifuging. Nucleic acids were precipitated with 70% isopropanol at −20°C overnight, then the supernatant was removed and the pellets were washed twice with 70% ethanol. Finally, the isolated DNA was dried and suspended in Tris-EDTA buffer. The contamination tests, integrity and purity of the isolated DNA were assessed as reported previously.
Sequencing on Illumina MiSeq platform. For diet characterisation, we used metabarcoding (Taberlet et al.

Diet of Lepus europaeus on Vesuvius
2007) and high-throughput sequencing (HTS) technology on faecal DNA. In particular, we followed the protocol reported by Buglione et al. (2018). The PCRs were performed in 25 uL final volume reactions, with 50 ng of DNA as the template, 1 U of Taq Solis polymerase (BioDyne, Tartu, Estonia), 2.5 uL of 10X Buffer B (0.8 M Tris-HCl, 0.2 M (NH 4 ) 2 SO 4 , 0.2% w/v Tween-20), 2.5 uL of 25 mM MgCl 2 , 4 uL of 2.5 mM deoxyribonucleotide triphosphate (dNTP) mix and 2.5 uL of 25 uM of JK11 and JK14 primer mix (Yokota et al. 1989;Aceto et al. 1999;Frediani & Caputo 2005). The primers were modified with a specific adapter to the 5ʹ ends, aimed at recovering sequences from sample post-sequencing (Coissac 2012). Four independent PCR replicates were performed for each sample. To monitor the performance of the process and check for potential contamination, negative (pure water) controls were included in each amplification, as well as positive controls (a DNA mix containing 50 ng/ uL from five plant families: Asteraceae, Fabaceae, Orchideaceae, Poaceae and Rosaceae). The PCR conditions were set up as an initial denaturation step for 3 min at 94°C, followed by 30 sec at 94°C, 45 sec at 52°C, and 45 sec at 72°C for 35 cycles, and, finally, an extension step for 7 min at 72°C.
After purification with Illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Buckinghamshire, UK), PCR replicates were mixed in equimolar concentrations in all PCR samples. Finally, these were mixed to generate a unique pool representative of the European hare diet on the study area. The pool was tagged for a library with internal positive and negative controls to check for contamination, sequencing a sample containing a known microorganism. The library was tested using TapeStation (Agilent Technologies, Santa Clara, CA).
Processing of sequencing data. The quality control check of the short HTS raw reads was performed with the FastQC software v. 0.11.4 (http://www. bioinformatics.babraham.ac.uk). Subsequently, we trimmed and cropped the reads with a phred quality score of Q < 28 and a minimum length of 35 nucleotides, and clipped the primers and Illumina adapters using the Trimmomatic software v. 0.35 (Bolger et al. 2014). We removed the orphan reads and simultaneously processed for the analysis only paired reads, checking the filtering performance using, again, the FastQC program. The filtered reads were used for the assembly to create overlapping and contiguous sequences (contigs) with the SOAPdenovo2 127-mer (Luo et al. 2012) software. Then, we assigned each sequence to its taxonomic level by blasting against all nucleotide records at National Center for Biotechnology Information (NCBI) using the BLAST 2.3.0+ software (Camacho et al. 2009), selecting only the alignments with an E-value < 0.05, an identity >80.0%, and an alignment score >30%. We increased the accuracy of the automatic taxonomic assignation, filtering the blast results using a regional list of plant species (Ricciardi et al. 2000(Ricciardi et al. , 2016 (www.actaplantarum. org). When the same contig was assigned to more than two taxa, we selected the higher taxonomic level including all of them. We tagged as unclassified the sequences assigned only to a taxonomic level higher than family.
The mapping was performed using the bwa-0.7.12 ), samtools 1.3 ) and samstat 1.5.1 (Lassmann et al. 2011) software programs, aligning the reads against the reference contigs, previously assigned to the respective taxa during the blast analysis. Supplementary and secondary alignments were detected and removed with samtools 1.3 (see SAM Alignment/ Map Format Specification at https://github.com/sam tools/hts-specs). Finally, for each taxonomic assignment, we calculated the number of corresponding reads, retaining sequences with read counts > 2 for the barcoding identification (Mollot et al. 2014).

Results
We collected 50 faecal samples (10 pellets per month), of which 42 were genetically assigned to the European hare. For eight samples (one from April; one from May; two from June; four from July) species assignment was not possible because of DNA amplification failure, thus these were excluded for the subsequent analyses. For diet characterisation, we used DNA (N = 42) with a concentration of ≥ 50 ng/μL and both λ 260/280 and λ 260/ 230 > 1.60.
From 2,563,506 Illumina sequencing reads (35-301 bp), we obtained a total of 696,618 contigs, of which 621,215 found a match with blast records. At the end of the filtering, a total of 76,561 sequences had resulted from bioinformatic processing of data: 6.01% were identified to the family level, 23.90% were identified to the genus level and 70.08% were identified to the species

1206
M. Buglione et al. level. No contamination was detected in the sequencing of the positive and negative controls. The plants identified at these three taxonomic levels are included in 30 families. The Fabaceae, Polygonaceae and Poaceae (counts > 3500) were listed as the most representative, whereas the other families were represented in low percentages (<1%) (Figure 2 and Table I).
In particular, Fabaceae (86.26% of the total diet) showed major variability in terms of observed items (n = 15; excluding plant elements assigned only to family level), with Galega officinalis and Lupinus angustifolius accounting for more than half of the total diet (67.10%). Next, Polygonaceae (Rumex sp.) and Poaceae amount to 12.10% of the total diet. The remaining 1.64% of the total reads assigned accounted for 27 families, among which frequencies of occurrence of 0.82%, 0.18% and 0.16% were found for Amaryllidaceae, Orchidaceae and Liliaceae, respectively; meanwhile 0.46% included the other 24 families expressed at the lowest percentages (<0.07%).

Discussion
The European hare is an interesting herbivore to study considering its adaptability, with a broadspectrum diet that changes in composition according to the diverse environments it colonises. Various studies have defined the variability of its diet in relation to different, even particularly extreme, environmental contexts (Puig et al. 2016(Puig et al. , 2017Naldi et al. 2020), but no one has ever taken into consideration the edibles of the species in a volcanic scenario.  Here, we analysed the diet of the European hare by using, for the first time on this species, a DNA metabarcoding approach, widely adopted for the characterisation of animals' food intake (i.e. De Barba et al. 2014;Kartzinel et al. 2015;Goldberg et al. 2020;Buglione et al. 2020aBuglione et al. , 2020c, allowing us to overcome many limitations related to the morphological method even if it is not without criticism (Holechek et al. 1982;Deagle et al. 2009;Soininen et al. 2009;Pompanon et al. 2012;Ingerson-Mahar 2014).
The diet of the hare on Vesuvius is mainly composed of herbaceous species, also commonly found along the roads, in uncultivated farmland and as pests in cultivated land, according to the dietary and environmental preferences reported in other studies (Reichlin et al. 2006;Karmiris et al. 2011;Schai-Braun et al. 2013, 2015Buglione 2017). This result supports the interpretation of a food strategy that tends to avoid elements difficult to digest, such as woody parts, which contain higher proportions of less-digestible fibres compared with leaves (Dierenfeld et al. 2000). This could also provide the advantage of minimising the amount of ballast (Cheeke 1999), and hence also the weight handicap in the hare, which requires high running speeds to avoid predation (Hackländer et al. 2002).
The most frequent items ingested by the hare are goat's rue (Galega officinalis) and blue (or narrowleafed) lupin (Lupinus angustifolius). The former is usually present in Mediterranean and submediterranean areas of Italy below 1000 m a.s.l. in natural pasture and at the edge of cultivated fields (Lastrucci et al. 2010).
The vegetational and floristic characterisations available for the Vesuvian areas have not reported goat's rue (Pasquale 1840;Licopoli 1873;Ricciardi et al. 1986Ricciardi et al. , 2000Ricciardi et al. , 2016, probably because such botanical studies devoted more attention to the native flora. Furthermore, low quantities of this species in historical times could explain its absence from the floristic-vegetational checklists. Indeed, intensive agriculture has been practised on the lower reaches of the slopes of Somma-Vesuvius since ancient times. However, these crops have undergone a progressive abandonment leading to a significant increase in vegetation with a low degree of naturalness, typical of ruderal areas and environments with a high degree of disturbance (Ricciardi et al. 2000).
The large amount of Galega officinalis in the diet could be traced back to a strategy used by females to stimulate milk production during lactation time, which occurred during our sampling period. In fact, a daily and controlled diet including goat's rue is known to increase milk yield in animals, such as cows, sheep and rabbit (González-Andrés et al. 2004;Penagos Tabares et al. 2014;Pałka et al. 2019;Darbyshire et al. 2022). However, it is not possible to know with certainty whether the presence of Galega officinalis was actually connected to the need for stimulating lactation in hare females because we did not determine the sex of the collected individuals and we pooled the samples for diet analysis. This opens the way for further investigations aimed at elucidating this aspect.
Furthermore, in some countries, goat's rue has been included in lists of plants toxic to livestock (Parton & Bruere 2002), because of the potential poisoning effect due to the presence of some alkaloids, mainly galegine and derivatives (Patterson 1992;González-Andrés et al. 2004). Thus, learning more about the systems used by the European hare to bypass such toxicity could be useful for optimising its use as forage for other animals (Peiretti & Gai 2006) or for human treatments, even if it is an exclusive adaptation developed by the European hare in this particular environmental context. Blue lupin is a domestic legume crop species producing large seeds with up to 40% protein content. Different from wild lupins, in the domesticated forms, mainly growing at high altitudes as blue lupin on Vesuvius, seeds are less bitter and toxic because of minor quinolisidine alkaloid contents (below 0.02%) (Wink et al. 1995;Gresta et al. 2017). This sweet lupin is an attractive food source for various mammals, such rabbits and hares . The lupin seeds, thanks to the lipid fraction, can be considered to fulfil an energy quota in animal nutrition (Hansen & Czochanska 1974;Musco et al. 2017). The hares prefer the parts of plants rich in fat, increasing energy assimilation, reducing foraging activity and producing milk with a high fat content (Hackländer et al. 2002;Smith et al. 2005).
The spectrum of available plants for hare on Vesuvius includes also other wild (i.e. Lolium sp., Bromus sp., Rumex sp.), cultivated (i.e. Solanum lycopersicum, Cucumis melo, Brassica oleracea, Allium cepa, Pisum sativum) or ornamental plant (i.e. Hypericum sp., Potentilla sp.) items (Pasquale 1840;Licopoli 1873;Ricciardi et al. 1986Ricciardi et al. , 2000Ricciardi et al. , 2016. The animals would have easy access to these resources, for example in plantations or farms, because they usually travel up to 1.8 km in search of suitable feeding areas, and may travel up to 15 km in one night while eating (Chapman & Flux 1990). However, these items are found only in trace quantities in the diet. All of this seems to suggests that although hares have the potential to take advantage of humanmanaged sites, they seem to persist in areas of volcanic environments, most frequently consuming plant species adapted to these soils, which sometimes are not the most abundant plants in the study area. To interpret these observations in the light of selective feeding behaviour, further investigations must be planned, also taking into account the movements of animals in volcanic areas, for example with video-trapping or radiotracking.
The hare on Vesuvius plays a very important role, especially as prey for sedentary or migrating birds that use the Gran Cono as a resting place. Therefore, adequate management of the European hare is necessary, aiming at the maintenance of the species in a balanced relationship with the other components of the ecosystem. This can be achieved through the increasingly refined study of this population, to acquire basic information for understanding which elements of the environment to pay most attention to.
Last but not least, we must not overlook the potential role that the European hare could play in affecting the plant population dynamics and community structure through selective grazing and/or by the phenomenon of endozoochory (Chang et al. 2005;Green & Pickering 2013;Nomura & Tsuyuzaki 2015). In particular, this leads to a dispersal of seeds where the parental patches are not established, and pellets provide the gradual release of a long-term supply of seeds even in a fluctuating and disturbed ecosystem, such as on a volcano (Green & Pickering 2013). It would be interesting to investigate the possible role of the European hare on Vesuvius in the recolonisation process of the vegetation after the extensive fire affecting the area in 2017.

Disclosure statement
No potential conflict of interest was reported by the authors.