The complete mitochondrial genome sequence of the Metschnikowia bicuspidata (Metschnikoff, 1884), an emerging pathogen of ‘milky disease’ in Chinese mitten crab

Abstract The outbreak of milky disease of Chinese mitten crab caused by M. bicuspidata seriously restricted the development of the crab industry. In this study, the mitochondrial genome sequence of M. bicuspidata was assembled, annotated, and further analyzed. The results indicated that the complete mitochondrial genome of M. bicuspidata was 75,095 bp, which contained two rRNAs, 23 tRNAs, and 13 protein-coding genes. The phylogenetic tree of 13 yeasts based on the complete mitochondrial genome was constructed which showed that M. bicuspidata (accession number OK514652) and M. bicuspidata (accession number MW147605.1) were clustered in a clade. To sum up, our research results would further provide essential data for the systematics and evolution study of M. bicuspidata.

Chinese mitten crab (Eriocheir sinensis) belongs to the phylum Arthropoda, class Crustacea, order Decapoda, family Grapsidae, and genus Eriocheir. It is an important economic crab in China (Fu et al. 2017). In 2018, the total production of E. sinensis reached 692,723 tonnes in China, with an output value of 9,386,536 thousand dollars (http://www.fao.org/fish ery/statistics, 2018). At present, the breeding areas of E. sinensis in China are mainly concentrated in Jiangsu, Hubei, Anhui, and Liaoning provinces. However, with the continuous expansion of the scale and density of crab farming, the disease has been one of the most important limitations of the sustainable development of the industry (Sui et al. 2012;Ding et al. 2015Ding et al. , 2016Bao et al. 2021). In 2019, a pathogenic yeast was isolated from diseased Chinese mitten crab in many farms of Panjin City, Liaoning Province, China (121 25 0 -122 31 0 E, 40 39 0 -41 27 0 N), which was identified as Metschnikowia bicuspidata (Metschnikoff, 1884) belongs to family Metschnikowiaceae, genus Metschnikowia (Ma et al. 2020(Ma et al. , 2022Bao et al. 2021). However, the mitochondrial genome sequence of M. bicuspidata was still in the blank.
The specimen of M. bicuspidata (Number: 2EJM001) was stored in the Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University, Dalian, China. We constructed the library with an average length of 350 bp using the NexteraXT DNA Libraries Preparation Kit (Illumina, San Diego, CA, USA) after the libraries were sequenced on Illumina Novaseq 6000 platform. The raw data totaled 2.84 G, and the clean data totaled 2.83 G after quality control processing and yielding a 1231-fold depth of coverage of the mitochondrial genome. The GC content of the clean data was 46.5%, the Q20 value was 97.90%, and the Q30 value was 93.96% which indicated that the quality of the mitochondrial genome sequencing and assembly results was very high. High-quality reads were assembled into the mitochondrial genome using de novo assembler SPAdes v.3.11.0 software (Bankevich et al. 2012). Finally, we annotated the assembled complete mitochondria genome by MITOS (Bernt et al. 2013). The sequence of M. bicuspidata mitochondrial genome was deposited in GenBank (accession number OK514652). Phylogenetic relationships between M. bicuspidata and other 12 yeasts were analyzed according to the whole mitochondrial genome from GenBank using Maximum Likelihood methods by MEGA 7.0.
The resulting phylogenetic tree showed that there were two main clades, Metschnikowiaceae and Sporidiobolaceae. Twelve yeasts species were clustered together within Metschnikowiaceae; Metschnikowia bicuspidata (accession number OK514652) and Metschnikowia bicuspidata (accession number MW147605.1) were clustered in a clade (Figure 1). In conclusion, the mitochondria of M. bicuspidata reported in this study provided more essential information about the yeast, which will help our understanding of M. bicuspidata better. Further, the information will do good to develop the rapid detection methods which will favor the control of the 'milky disease' in Chinese mitten crab.

Storage location of the specimen or its DNA
The specimen of M. bicuspidata (Number: 2EJM001) was stored in the Aquatic Animal Hospital of Dalian Ocean University, Dalian Ocean University, Dalian, China. And the email of Shigen Ye, the person in charge of the collection, is shgye@dlou.edu.cn.

Acknowledgments
We thank Baoshi Guan for his kind help in mitogenome data analysis.

Author contributions
Shigen Ye: conception and design. Jiannan Liu, Jieying Yu, Yihao Li, Xinran Shi, Senting Pu, and Xiaoran Zhao: analysis and interpretation of the data. Jiannan Liu: draft the paper. Shigen Ye: revising it critically for intellectual content. Shigen Ye: the final approval of the version to be published. And all authors to agree to be accountable for all aspects of the work.

Disclosure statement
No potential conflict of interest was reported by the author(s).