The complete mitogenome of Chlorophanus auripes Faust, 1897 (Coleoptera, Curculionidae)

Abstract Chlorophanus auripes Faust, 1897 is a large size weevil dwell in biotopes on Salix spp. or Populus spp., which can be found common in Northern China. The complete mitogenome of C. auripes was reported in the present study. The mitogenome contains 18,149 base pairs. The composition of mitogenome is 39.4% for A, 10.1% for G, 36.0% for T and 14.5% for C. A set of 37 genes, including 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes and one control region, were annotated. There is a 1037 bps gap between Ile tRNA gene and Gln tRNA gene. The phylogenetic result supports the monophyly of Curculionidae, revealed that C. auripes was a sister group to the Pachyrhinus yasumatsui, which also belongs to the subfamily Entiminae.


Introduction
Chlorophanus is a large weevil genus contains nearly 100 species, which belongs to the subtribe Tanymecina Lacordaire, 1863 of tribe Tanymecini Lacordaire, 1863 in subfamily Entiminae Schoenherr, 1823. They are large size (6.9-14 mm) and with a body being usually covered with green scales (Legalov 1997). The C. auripes is distributed in Northern China, Japan, and Korea (Han 2014). They dwell in biotopes on Salix spp. Or Populus spp. (Reitter 1915).

Results and discussion
The complete mitogenome of C. auripes is a circular DNA molecule with a length of 18,149 base pairs and contains 37 genes including 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and one control region. Its sequence was deposited in GenBank under the accession number OM112264. The phylogenetic relationship was constructed based on the 13 protein-coding genes from 51 species of Curculionidae and 1 outgroup species of Meloidae using the IQ-tree on XSEDE with the maximum-likelihood method (Figure 1). The result highly supported the monophyly of Curculionidae and showed that C. auripes was the sister group of Pachyrhinus yasumatsui, which is consistent with the result based on 28S rDNA gene, CAD gene, ArgK gene, EF-1a gene, and COI gene (Jordal 2011).

Ethical approval
This study was approved by the Administration Committee of Experimental Animals, Qinghai Province, China.

Author contributions
Xiaoning Zhang was involved in the conception and design. Guoxiang Zhang was involved in the PCR experiments. Qingbai Hou was involved in the analysis and interpretation of the data. All authors contributed to the final manuscript and agree to the final approval of the version to be published. All authors agree to be accountable for all aspects of the work.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Funding
The author(s) reported there is no funding associated with the work featured in this article.