The complete mitochondrial genome of the Amur soft-shelled turtle (Pelodiscus maackii Brandt, 1858), from South Korea

Abstract In this study, we use a specimen from wild-caught individual to determine the complete mitochondrial genome of the Amur soft-shelled turtle (Pelodiscus maackii). The complete mitogenome of P. maackii has 16,258 bp in length and consists of 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and one control region. The arrangement of genes of P. maackii is identical with previously reported mitogenomes in the family Trionychoidea. According to our result, the ML tree for the phylogenetic reconstruction revealed that the individuals used in present study is closely related with the previously reported sequences of P. sinensis (AY962573 and MG431983) in p-distance 0.7% and 2.5%.

The genus Pelodiscus is comprised of five species worldwide. In South Korea, two species of Pelodiscus are distributed, Amur soft-shelled turtle (Pelodiscus maackii Brandt, 1858) and Chinese soft-shelled turtle (Pelodiscus sinensis Wiegmann, 1835). P. sinensis and P. maackii are commonly confused in species-level identification because of their high morphological similarities. However, there are several morphological keys to distinguish (Chang et al. 2012;Farkas et al. 2019). Generally, P. maackii is known to have longer carapace length than P. sinensis and P. maackii has a no pattern and unmarked plastron with white to straw yellow. Besides, P. sinensis has a no pattern or relatively small, faint round to oval dark markings with snow white to pinkish white (Farkas et al. 2019). Even though P. sinensis is known as introduced species (Chang et al. 2012), the definite status of Pelodiscus species distributed in Korean peninsula is still controversial. So far, previous studies on Pelodiscus species in South Korea are very insignificant, especially in genetic studies, and the study on the mitogenome of P. sinensis using specimen collected from soft-shelled turtle farm is the only research conducted (Jung et al. 2006). Therefore, in this study, we conduct mitogenome research on the specimen collected in the natural state to clarify the species distribution state of Korean soft-shelled turtle and to confirm the phylogenetic status with related species.
To reconstruct the phylogenetic tree for P. maackii, 14 related species in the same superfamily Trionychoidea were used and the Carettochelys insculpta (FJ862792) belonging to Carettochelyidae was used as outgroup. DNA molecular phylogeny was reconstructed with 1,000 replication of bootstrapping by maximum likelihood (ML) methodologies with MEGA 7.0 (Kumar et al. 2016). The best fit model was chosen on the basis of Akaike information criterion (AIC) as formed in ModelTest-NG v0.1.6 in CIPRES (Miller et al. 2010), and GTR þ I þ G model was selected. For the phylogenetic reconstruction of 14 related species in Trionychoidea was well supported (BP >70%) and performed monophyly. Our findings from the present study coincide with the results from Li et al. (2017), which uses complete mitogenome of 14 species in Trionychoidea with minor difference caused by different methodologies used.
As a result, the specimen of P. maackii used in the present study is distinct, but almost identical with P. sinensis (AY962573) from the soft-shelled turtle farm in South Korea with a 0.7% of genetic distance (Figure 1). The other P. sinensis (MG431983) from China showed a 2.5% of genetic difference with the specimen used. The mean interspecific divergence in the genus Pelodiscus is 2-3% (Yang et al. 2011;Suzuki and Hikida 2014), which is relatively low compared to the other genera in Trionychoidea. Since the specimen of P. sinensis (AY962573) used in the present study has been reseparated into P. maackii by Fritz et al. (2010) using molecular makers and considering the finding of this research showing a similar result, it is reasonable to identify P. sinensis (AY962573) as P. maackii. This research is the first and only mitogenome study conducted on wild P. maackii in South Korea. Therefore, it is expected to make a great contribution to providing basic genetic data for the future studies of P. maackii.

Ethical consideration and permissions
The experiment was conducted in accordance with the standard operating guidelines of the animal testing and/or laboratory animal related committee (IACUC)

Disclosure statement
No potential conflict of interest was reported by the authors.

Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. OK377340. The associated BioProject, SRA and Bio-Sample numbers are PRJNA768941, SRX12511000 and SAMN22071900 respectively.