The complete mitochondrial genome of basiprionota bisignata (Boheman, 1862) (Coleoptera: Chrysomelidae)

Abstract The complete mitochondrial genome of Basiprionota bisignata (Boheman, 1862) (a species of leaf beetles) was successfully sequenced, annotated, and analyzed in this study. This mitochondrial genome is a circular DNA molecule of 16,069 bp in size with 78.5% AT content, including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich region (control region). The gene order is consistent with the putative ancestral arrangement of insects. All PCGs are initiated by ATN (A/T/C/G) condons and terminated with TAA/G or their incomplete form single T-. All tRNAs can be folded into common clover leaf secondary structures, except for trnS1. The phylogenetic tree was reconstructed using maximum likelihood analysis, and the topology recovered the monophyly of Cassidinae and the sister relationship between Basiprionota and the clade (Thlaspida + Aspidomorph).

The genus (Basiprionota Chevrolat, 1837) belongs to the subfamily Cassidinae of Chrysomelidae, comprising 63 species distributed in Oriental region and border parts of Palearctic and Australopapuan regions (Borowiec 1999;Borowiec and Swie R toja nska 2002). In this study, we sequenced the complete mitochondrial genome of Basiprionota bisignata (Boheman, 1862), the first representative of Basiprionota, and performed a phylogenetic analysis among Chrysomelidae with the available mitogenomic sequences.
The complete mitochondrial genome of B. bisignata is 17,116 bp in length, containing 13 protein-coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an AT-rich region (control region). The gene order of the newly sequenced mitochondrial genome is consistent with the putative ancestral arrangement of insects (Clary and Wolstenholme 1985;Cameron 2014). The AT content of the mitochondrial genome is 78.5% (A ¼ 42.8%, T ¼ 35.7%, C ¼ 12.8%, and G ¼ 8.7%), which has a strong AT nucleotide bias. Most PCGs (nad2, cox1, atp8, atp6, nad3, nad4, nad4L, nad6, cytb, and nad1) share typical stop termination TAA/G, whereas cox2, cox3, and nad5 end with incomplete form single T-. Furthermore, all PCGs use ATN (A/T/C/ G) as start codon. Length of the 22 tRNAs ranges from 60 bp (trnE) to 68 (trnM). All tRNAs can be folded into common clover-leaf secondary structures, except for trnS1, in which the dihydrourine (DHU) arm formed a simple loop. The size of 16SrRNA and 12SrRNA is 1,259 bp and 740 bp, respectively. The AT-rich region is located between 12SrRNA and trnI, which is 2,515 bp in length with an AT content of 82.8%.

Ethical approval
Experiments were performed in accordance with the recommendations of the Ethics Committee of Guizhou Academy of Forestry. These policies were enacted according to the Chinese Association for the Laboratory Animal Sciences and the Institutional Animal Care and Use Committee (IACUC) protocols.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. MZ699993. The associated BioProject, SRAs, and Bio-Sample numbers are PRJNA759086, SRR15686511-SRR15686512, and SAMN21155885, respectively.