The complete mitochondrial genome of the cocoa tussock moth Orgyia postica Walker, 1855 (Lepidoptera: Lymantriidae)

Abstract The high-throughput sequencing method was used for the first time to determined complete mitochondrial genome of Orgyia postica. The complete mitochondrial genome was a circular molecule with 15,258 bp in full-length, including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs) and 2 ribosomal RNA genes (rRNAs). The nucleotide composition of O. postica mitogenome was A: 37.6%, C: 7.9%, G: 14.8%, T: 39.7%. In addition, the results of the phylogenetic analysis indicate that O. postica had the closest relationship with Laelia suffusa. Our study will contribute to further research on the evolution of Lymantriidae and the identification of larva species.


Introduction
Orgyia postica Walker, 1855 (Lepidoptera: Lymantriidae) is a crop pest that mainly damages stamens and leaves, It mainly damages more than 70 crops such as tea, cotton, strawberries, loofah, asparagus, radishes, peaches, grapes, pears, citrus, mangoes, causing poor growth, reduced production and even death (Luo, 2016). The sequence of the mitochondrial genome is useful for a deeper understanding of the evolution and identification of insects (Du, 2017;Wang et al., 2019;Chen et al., 2020;Su and Wang, 2020;Dong et al., 2021). Here, we report the complete mitochondrial genome sequence of O. postica, in order to better understand the relationship between O. postica and related genera, and contribute to understanding the evolution and identification of Lymantriidae.
The sample of O. postica was collected at Taxidermy Park in Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China (22 50 0 N, 108 17 0 E). The specimen was deposited at the Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety (Nanning, China) (Voucher specimen number: FDSW202318382-1r) and identified by the head of herbarium Mr Li (Email: lijunlijun1981@ 163.com). Genomic DNA was extracted using Genomic DNA Kit (TransGen Biotech, Beijing) and constructed the libraries with an average length of 350 bp using the NexteraXT DNA Library Preparation Kit (Illumina, San Diego, CA). Then the libraries were sequenced on Illumina Novaseq 6000 platform, 9.23 Gb clean data was assembled by SPAdes (version 3.11.0) (Bankevich et al., 2012) and annotated by MITOS (http:// mitos.bioinf.uni-leipzig.de/index.py). The original sequencing data were uploaded to the NCBI database (https://www.ncbi. nlm.nih.gov/sra/) with the accession number SRR15041082. The complete mitochondrial genome was assigned Genbank accession number MW355619.
To  (Katoh and Standley, 2013). The evolutionary history was inferred by using the Maximum Likelihood method with 1000 bootstrap replicates based on the Tamura-Nei model (Tamura and Nei, 1993). The tree with the highest log likelihood (À217,907.0919) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 22 nucleotide sequences. Codon positions included were 1st þ 2nd þ 3rd þ Noncoding. There was a total of 36,952 positions in the final dataset. Evolutionary analyses were conducted in MEGA7 software version 7.0.18 (Kumar et al., 2016). By using various other families of the order lepidoptera as outgroup we got the final ML tree, then Figure 1 shows that L. suffusa has the closest relationship with O. postica.

Disclosure statement
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the article.

Funding
The experiment was financially supported by National Natural Science Foundation of China [32060255].

Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/ under the accession no. MW355619. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA743722, SRR15041082, and SAMN20058967, respectively.