The complete chloroplast genome of Lonicera pampaninii Levl. and its phylogenetic analysis

Abstract Lonicera pampaninii Levl, a Chinese herbal medicine widely used in the folk, has the effect of clearing away heat and detoxifying similar to other plants of the Lonicera. However, its genetic relationship with these plants is unclear. In this work, the cp genome of Lonicera pampaninii Levl. was assembled by the high-throughput Illumina pair-end sequencing data. The circular cp genome is 155,249 bp in size, including a large single-copy (LSC) region of 89,068 bp and a small single-copy (SSC) region of 18,635 bp, which were separated by two inverted repeat (IR) regions (23,773 bp each). A total of 120 genes were predicted, including eight ribosomal RNAs (rRNAs), 33 transfer RNAs (tRNAs), and 79 protein-coding genes (PCGs). Furthermore, phylogenetic analysis revealed a strong sister relationship between L. pampaninii and other two congeneric species (Lonicera confusa and Lonicera japonica). This study provides useful information for future genetic study of L. pampaninii.

Lonicera pampaninii Levl. is commonly known as 'chicken bone tree', belongs to the family Caprifoliaceae. It has the effects of expelling wind, clearing up heat and detoxification and commonly used to treat colds, coughs, sore throat, red eyes, swelling and pain, lung carbuncle, breast carbuncle, wet sores. L. pampaninii is mostly distributed in North China, Shaanxi, Gansu, Hubei, Sichuan, Yunnan, Guizhou and other regions with large reserves (Wei et al. 1999). In addition, the stems of L. pampaninii can also be used as a material to make artificial cotton. The soil of seed from L. pampaninii can make soap, and its vine can be used for garden greening (Duan et al. 1986;Wei et al. 1999). It is reported that luteolin, inositol, saponins, tannins, chlorogenic acid, isochlorogenic acid and other chemical components have been isolated from Lonicera japonica, and the content of chlorogenic acid has been determined (Peng et al. 2000;Xu et al. 2007;Lin et al. 2008;Zheng et al. 2012;Xiong et al. 2013;Yu et al. 2015;Kong et al. 2017). The chemical composition of L. pampaninii is similar to Lonicera japonica according to previous reports (Duan et al. 1986). Moreover, most researches show that caffeoylquinic acid, cerebrosides, nitrogen-containing iridoid glycosides and triterpene glycosides from Lonicera japonica have anti-allergic, anti-inflammatory, antibacterial and anti-bacterial antiviral activities. Although L. pampaninii has high economic value, few studies have focused on genetics of this plant for its distribution area and hidden growth environment. In this work, the complete chloroplast (cp) genome of L. pampaninii has been assembled and determined. The study provides useful information for future genetic research of L. pampaninii.
Young and healthy leaf samples were collected from Dangwu Town, Huaxi District, Guiyang City, Guizhou province, China (26.39005 N,106.612275 E, 1197.5 m above sea level). The plants were identified using a species identification key by Dr. Chunyan Han, Kunming Caizhi Biotechnology Co. Ltd, Kunming, Yunnan, China. The leaf specimen (accession number: GZNUYZW202101001) was deposited in the herbarium of School of Life Sciences, Guizhou Normal University. The total genomic DNA (No. YX20210115901) was extracted using E.Z.N.A Plant DNA kit (FEIYANG, Guangzhou, China) and stored at À80 C in the laboratory (room number: 1403) of School of Life Sciences, Guizhou Normal University. The genomic DNA of the sample is tested, and the DNA is interrupted by physical methods (ultrasound) after passing the test. The interrupted DNA is purified to construct a sequence library.First, extract the phyllochondrial genomic DNA, then use 1% agarose gel electrophoresis to detect and collect the phyllochondrial genomic DNA, and finally build a library with an initial amount of 1000 mg DNA.Break the total DNA into 400 bp fragments, filter reads with low sequencing quality, andfilter duplication reads to obtain high-quality clean data. Genomic DNA was extracted from fresh leaves and then sequenced using the Illumina NovaSeq platform (Illumina, San Diego, CA). The filtered reads were assembled using the program GetOrganelle (Jin et al. 2020) with Lonicera japonica (GenBank accession number: MH028738) as the initial reference genome. The assembled cp genome was annotated using the online software GeSeq (Tillich et al. 2017) by comparing the sequences with the cp genome of Lonicera japonica. The accurate annotated complete cp genome was submitted to GenBank with accession number MZ241298.
To further understand the cp genome of L. pampaninii, 21 cp genome sequences of Caprifoliaceae family (one Triosteum species, 13 Lonicera species, one Weigela species, two species from Dipelta genus, one Heptacodium species, and three species from Patrinia genus,) were downloaded from GenBank to construct the phylogenetic trees through maximum-likelihood (ML) analysis. The ML tree based on GTR þ gamma þ I model was performed using RAxML (Version 8.0.19) with 1000 bootstrap replicates (Stamatakis 2014). The phylogenetic tree indicated that L. pampaninii belongs to Lonicera genus (Figure 1), and showed a strong sister relationship between L. pampaninii and other two congeneric species (Lonicera confusa and Lonicera japonica).

Disclosure statement
No potential conflict of interest was reported by the author(s).

Funding
This study was supported by the National Natural Science Foundation of China under Grant [Nos. U1812401 and 32060068].

Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov, reference number MZ241298. The associated BioProject, SRA, and Bio-Sample numbers are PRJAN752821, SUB10196548, and SAMN20608006 respectively.