The complete mitochondrial genome of the freshwater fish Opsariichthys uncirostris amurensis from Korea

Abstract We present the first report of the complete mitochondrial genome of Opsariichthys uncirostris amurensis, which consists of 16,613 base pairs harboring 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region (D-loop). The overall base composition of the complete genome is A (27.15%), C (27.15%), T (26.77%), G (18.92%). The complete mitogenome of O. uncirostris amurensis, which was most closely related to O. bidens in the Bayesian inference tree, provides a better understanding of the phylogeny of the genus Opsariichthys.

Opsariichthys uncirostris amurensis (Berg 1932) (Cypriniformes: Cyprinidae) is a subspecies of O. uncirostris. Opsariichthys uncirostris amurensis lives in most rivers and streams in the Korean Peninsula, except for those flowing to the East Sea of Korea (Kim and Park 2002). Opsariichthys uncirostris amurensis has been studied only in phylogenetic analyses of the genus Opsariichthys; studies of O. uncirostris amurensis are lacking (Okazaki et al. 2002). This study is the first to report the complete O. uncirostris amurensis mitogenome and provides a better understanding of the phylogenetic relationships in the genus Opsariichthys.
A specimen of O. uncirostris amurensis was collected from Jicheon Stream (36 19'N, 126 55'E) flowing into the Geumgang River in Korea on 18 May 2018, and was preserved in 99.9% ethanol. The specimen was given voucher number SUC-25115 and stored in the fish specimen room of Soonchunhyang University, Korea (Voucher Storage: Soonchunhyang University; Voucher number: SUC-25115; The person in charge of the collection: KR Kim; email: kimkan-grae9586@gmail.com). Genomic DNA was extracted from the caudal fin using the Genomic DNA Prep Kit (BioFact, Daejeon, South Korea) and stored in a deep freezer at À80 C. Genomic DNA was extracted from the caudal fin using the Genomic DNA Prep Kit (BIOFACT, Daejeon, South Korea). The extracted genomic DNA was stored in the specimen room of Soonchunhyang University. To obtain the complete mitochondrial DNA sequence, a DNA library was prepared according to the MGIEasy DNA Library Prep Kit (MGI Tech, Shenzhen, China) manual and subjected to 150 bp paired-end sequencing on the MGISEQ-2000 platform (MGI Tech). The next-generation sequencing raw data were assembled using Geneious 11.0.3, and the assembled mitogenome sequences were annotated using the MITOS web server (Bernt et al. 2013). The complete mitogenome sequence of O. uncirostris amurensis was deposited in the National Center for Biotechnology Information GenBank database under the accession number MW355488.
A phylogenetic tree was constructed based on the 13 PCGs using Bayesian inference in MrBayes 3.2.7 (Ronquist et al. 2012). For the dataset, we used the GTR þ IþG model proposed in jModelTest 2.1.10 (Guindon and Gascuel 2003;Darriba et al. 2012).
The results indicated that O. uncirostris amurensis is the most closely related to Opsariichthys bidens (G€ unther 1873). Opsariichthys uncirostris amurensis from the Korean Peninsula was expected to be close to O. uncirostris, which live in Japan, but was more closely related to O. bidens from China (Figure 1). When the sea level was low, the rivers flowing to the western part of the Korean Peninsula, eastern China, and western Japan were connected to each other through the paleo-Yellow River system. However, it is thought that the river flowing to western Japan was separated geographically due to the sea level rise, and the fish therein evolved into O. uncirostris (Okazaki et al. 2002;Takehana et al. 2004;Jung et al. 2009;Won et al. 2020).
For greater insight, a p-distance analysis was performed on O. uncirostris amurensis and species in the genus Opsariichthys.

Disclosure statement
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

Funding
This study was supported by Soonchunhyang University Research Fund.  1. A phylogenetic tree was constructed for the genera Opsariichthys, Zacco, Candidia, Nipponocypris, and Parazacco, with outgroup species and subspecies, using Bayesian inference based on 13 protein-coding genes. The numbers above the nodes are the posterior probabilities of the Bayesian analysis. Scale bars indicate the relative evolutionary distances. The species names are followed by their GenBank accession numbers.