The complete mitochondrial genome of Abramites hypselonotus (Günther, 1868) (Ostariophysi: Characiformes: Anostomidae): an aquarium headstander

Abstract Abramites hypselonotus, commonly known as marbled headstander, is an important freshwater aquarium fish from Brazil, found in the Orinoco, Amazon, Paraguay and lower Parana River basins. This genus has only two species and only this species occurs in Brazil. The complete mitochondrial genome of Abramites hypselonotus is 16,685 bp in length and it includes 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs genes and a control region with 1,028 bp. It has two PCGs with GTG start codon and the others with ATG start codon. Four of the 13 PCGs appear TAA stop codon, three incomplete TA_ stop codon, four incomplete T_ _ stop codon, one contain AGG stop codon and one TAG stop codon. Phylogenetic analysis showed that Abramites hypselonotus formed a sister group of Leporinus affinis (AP011994.1), thus maintaining the Family Anostomidae as a clade.

However, ecological research shows a low frequency of occurrence of this species in the natural environment (Begossi, et al. 1999;Pains da Silva et al. 2010;Horn et al. 2011;S uarez et al. 2013;Silveira and Weiss 2014). Considering that most of the specimens are caught in the wild for trade rather than captive breeding, a condition of overexploitation of specimens may lead to population decline (Cooney et al. 2015).
DNA was extracted from the muscle tissue of a specimen A. hypselonotus, acquired from an aquarium (S À19.9439W À43.9325), in the city of Belo Horizonte, Minas Gerais, Brazil and deposited in the vertebrates collection of the Federal University of the West of the Bahia (Voucher UFOB:VERT:0055), Bahia, Brazil. The genomic library was constructed and sequenced using MiSeq platform (Illumina, San Diego, CA.), with paired-end 150 bp strategy. The assembly was produced using Geneious Prime 2019.0.4.
The complete mitochondrial genome of A. hypselonotus (accession Genbank MW541938) was 16,685 bp in length. The base composition is estimated 29,69% for A, 28,17% for T, 26,41% for C and 15,75% for G, and the result of GC content was 42,14%. The mitochondrial genome was annotated using MitoFish webserver (Iwasaki et al. 2013) and Expasy Translation tool (https://web.expasy.org/translate/) (Artimo et al. 2012) was used for verification of the protein sequence. The mtDNA contain 13 protein-coding genes (PCGs), 2 rRNAs genes, 22 tRNAs genes and one control region.
The gene arrangement and coding bands were similar to the vertebrate standard mitogenome (Satoh et al. 2016). It has two PCGs with GTG start codon (CoxI and Nd4) and the others with ATG start codon. Four of the 13 PCGs contain TAA stop codon (Nd1, ATP8, Nd4L and Nd6), three contain incomplete TA_ stop codon (Nd2, ATP6 and CoxIII), four contain incomplete T_ _ stop codon (CoxII, Nd3, Nd4 and Cytb), one contain AGG stop codon (CoxI) and one TAG stop codon (Nd5). The incomplete stop codons were completed as TAA by post-transcriptional polyadenylation (Ojala et al. 1981). Intergenic regions ranged from 1 to 14 bp. Base overlap was found between the tRNA IIe and tRNA Gln (2 bp); tRNA Gln and tRNA Met (1 bp); CoxI gene and tRNA Ser (13 bp); ATP8 and ATP6 genes (10 bp); Nd4L and Nd4 genes (10 bp); Nd5 and Nd6 genes (4 bp); tRNA Thr and tRNA Pro (2 bp). Size of the tRNAs ranged from 66 to 74 bp and control region appeared 1,028 bp in length.
The mitogenome of Abramites hypselonotus, with 16,685 bp, is similar to other characiform fish, but it presents a difference in the number overlapping bases between the Nd4L-Nd4 genes (10 bp).

Acknowledgments
Federal University of the West of the Bahia (UFOB) and Federal University of Minas Gerais (UFMG) for the support during the project execution.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov under the accession no. MW541938. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA698912, SRR13614799, and SAMN17762312 respectively.