The complete mitochondrial genome sequence of Rana dabieshanensis (Anura: Ranidae)

Abstract Rana dabieshanensis is a species of frog within the family Ranidae. In this study, we assembled a complete mitochondrial genome (mito-genome) for R. dabieshanensis by high-throughput sequencing technology. It is 18,291 bp and includes 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one control region. The nucleotide composition is A: 27.0%, T: 28.6%, C: 29.3% and G: 15.2%. Two overlaps among the 13 protein-coding genes were found: ATP8/ATP6, ND4L/ND4. The study of phylogenetic analysis based on complete mitochondrial genome showed that there was close genetic relationship between R. dabieshanensis and R. omeimontis and it is useful for systematic analyses of genus Rana.


Rana dabieshanensis; mitochondrial genome; phylogenetic analysis
The Dabie mountain brown frog (Rana dabieshanensis) belongs to the family Ranidae, which is distributed in Dabie Mountains regions, Anhui Province, China . It is controversy about the classification of species within the genus Rana in the Dabie Mountains (Wang 2018). To better understand the mitochondrial genomic characteristics, phylogeny and evolution of the R. dabieshanensis, we determined and described the mitogenome sequence of R. dabieshanensis in order to obtain basic genetic information about this species.
A specimen of R. dabieshanensis was collected from Sucheng County, Anhui Province, China (31 31 0 13.61 00 N, 116 32 0 44.87 00 E) and stored in Anhui University Museum, Research Center for Biology (Voucher number: DBS202001). The genomic DNA extraction, library preparation and Illumina sequencing were done by Novogene Bioinformatics Technology Co. Ltd. (Tianjin, China).
We obtained the complete mitochondrial genome of R. dabieshanensis is 18,291 bp and submitted to the GenBank with the accession number MW526989. It included 13 protein-coding genes, two ribosomal RNAs genes, 22 transfer RNAs genes and one control region. The overall nucleotide composition is A: 27.0%, T: 28.6%, C: 29.3% and G: 15.2%, with a total A þ T content of 55.6%. The mitogenome of R. dabieshanensis shows the typical gene observed in Ranidae mitogenomes (Liu et al. 2017;Fang et al. 2020;Jiang et al. 2020). Within 37 mitochondrial genes, the ND6 gene and 8 tRNA genes (tRNA Ser , tRNA Glu , tRNA Pro , tRNA Gln , tRNA Ala , tRNA Asn , tRNA Cys and tRNA Tyr ) were encoded on the light strand and other genes were encoded by the H-strand. In 13 mitochondrial protein-coding genes, except COX1, ATP6 and ND4L begin with GTG, ND6 begin with TCT, the other nine genes begin with ATG as start codon. In all six types of stop codon were annotated, TAT for ND2, AGG for COX1 and ND5, TAG for ATP8, TAA for ND4L and CYTB, CAT for ND6 and an incomplete stop codon T for the remaining six PCGs (COX2, ATP6, COX3, ND3, ND4 and ND1), which is presumably completed as TAA by posttranscriptional polyadenylation (Ojala et al. 1981). With regard to the codon of ND6 and ND2, it is consistent with the Rana omeimontis submitted on NCBI (Jiang et al. 2020). Two overlaps among the 13 protein-coding genes were found: ATP8/ATP6 and ND4L/ND4. The two rRNA genes were 930 bp (rrnS) and 1576 bp (rrnL), respectively.
To best understand its phylogenetic position within the genus Rana, we reconstructed the phylogenetic tree of some species in Rana with maximum-likelihood method (ML) (http://iqtree.cibiv.univie.ac.at/), which is based on 13 complete mitochondrial genome sequences for Rana species. Another species (Pelophylax nigromaculatus) is included as the outgroup (Figure 1). The result showed that phylogenetic relationship of Dabie mountains wood frog is very close to R. omeimontis.

Disclosure statement
No potential conflict of interest was reported by the authors.

Data availability statement
The data that support the findings of this study are openly available in NCBI at https://www.ncbi.nlm.nih.gov/ (MW526989).