Complete mitochondrial genome of a predominant parasitoid, Necremnus tutae (Hymenoptera: Eulophidae) of the South American tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)

Abstract The complete mitochondrial genome of a predominant parasitoid, Necremnus tutae (Hymenoptera: Eulophidae) (GenBank accession number MT916846) is 15,252 bp in length, and contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes, and an A + T-rich region. The overall base composition is 38.86% for A, 7.14% for C, 8.57% for G, and 45.43% for T, with a high AT bias of 84.29%. ATA, ATT, ATG were initiation codons and TAA and T were termination codons. All the 22 tRNAs displayed a typical cloverleaf secondary structure, except for trnS1 and trnR which lacked the dihydrouracil (DHU) arm. Phylogenetic analyses were performed using 13 PCGs showed that N. tutae is closely related to Tenthredo tienmushana, which in accordance with the traditional classification.

The South American tomato leafminer, Tuta absoluta (Meyrick), is native to the western Neotropics. After invading Spain in 2006, it spread rapidly throughout Afro-Eurasia and has become a major threat to world tomato production (Campos et al. 2017;Biondi et al. 2018). Due to the increasing serious insecticide resistance, biological control using Hymenoptera parasitoids was becoming a major management measure of T. absoluta (Guedes et al. 2019;Gervassio et al. 2019). Necremnus tutae (Hymenoptera: Eulophidae) was a predominant larval parasitoid of T. absoluta (Gabarra et al. 2014), however, it was identified as a new species until 2015 (Gebiola et al. 2015). In this study, adult individuals of N. tutae were collected from the Institute for Research and Technology in Agriculture (IRTA), Cabrils, Barcelona (N41 30 0 , E2 22 0 ) in 2019. The specimens were preserved in 95% ethanol and deposited in our laboratory (Department of Biological Invasion, the Institute Plant Protection, Chinese Academy of Agricultural Science) with an accession number CAAS-IPP-DBI-Ntutae2019002.
The complete mitochondrial genome of N. tutae (GenBank accession number MT916846) is a circular DNA molecule of 15,252 bp in length, comprising 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rrnL and rrnS), and an A þ T-rich region (Boore 1999). Twenty-seven genes were encoded on the minority strand (N-strand), while the others were transcribed on the majority strand (J-strand). The overall base composition of N. tutae mitogenome is 38.86% for A, 7.14% for C, 8.57% for G, and 45.43% for T, with a high AT bias of 84.29%. The AT-skew and GC-skew of this mitogenome were À0.078 and 0.091, respectively.
Gene overlaps were found at 23 gene junctions and involved a total of 568 bp, and the longest overlap (235 bp) existed between trnW and trnM. There were five intergenic spacer regions ranging in length from 1 to 10 bp, comprising a total length of 21 bp. The largest intergenic spacer sequence of 10 bp was located between atp8 and atp6. The 22 tRNAs had a total of 1456 bp, and their individual lengths ranged from 61 bp (trnS 1 ) to 71 bp (trnT). All the 22 tRNAs displayed a typical cloverleaf secondary structure, except for trnR and trnS 1 lost the dihydrouridine (DHU) arm. The lengths of rrnL and rrnS were 1308 and 771 bp in length, with the A þ T contents of 87.23 and 88.2%, respectively. The A þ Trich region was located between rrnS and trnA with a length of 53 bp.
All the13 PCGs start with typical ATN codons, including two ATAs (nad1 and nad5), six ATGs (atp6, cob, cox1, cox3, nad4, and nad6), and five ATTs (atp8, cox2, nad4L, nad3, and nad2). Ten PCGs terminate with conventional stop codons (TAA or TAG), and the remaining PCGs including atp6, nad4, and nad6 use a single T as stop codon. According to the relative synonymous codon usage analyses of 13 PCGs, TTA (L), TTT (F), ATT (I), and ATA (M) were the four most frequently used codons. Leucine, isoleucine, phenylalanine, and methionine are the most frequent amino acid of 13 PCGs. Based on the concatenated amino acid sequences of 13 PCGs, the neighbor-joining method was used to construct the phylogenetic relationship of N. tutae with 18 other bees with MEGA7 (Kumar et al. 2016). The result showed that N. tutae was closely related to Tenthredo tienmushana (Figure 1), which agrees with the conventional classification.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Funding
This study was supported by National Key R&D Project of China [2017YFC1200603].

Data availability statement
The data that support the findings of this study are openly available in GenBank at https://www.ncbi.nlm.nih.gov/genbank/, reference number MT916846.