The complete chloroplast genome of Lemmaphyllum intermedium, a valuable medicinal fern

Abstract Lemmaphyllum intermedium is a valuable medicinal fern in China. We have determined its complete chloroplast genome using Illumina paired-end sequencing. The genome is 157,795 bp in length with 132 genes, including 88 protein-coding genes, eight ribosomal RNA genes, 35 tRNA genes, and one pseudogene. It has a quadripartite structure consisting of a small single-copy (SSC) of 21,764 bp, a large single-copy (LSC) of 81,189 bp, and two inverted repeats (IRs) of 27,421 bp each. A maximum-likelihood phylogenetic tree reconstructed by using complete chloroplast genome sequences reveals that L. intermedium is closely related to Lemmaphyllum carnosum var. microphyllum with strong support.

Lemmaphyllum intermedium (Ching) Li Wang, also known as Lemmaphyllum diversum (Rosenstock) Tagawa, is a vigorous fern belonging to Polypodiaceae ). The plant grows on rocks under the forest canopy at the altitude of 700 to 2200 m. Its rhizomes are covered with brown and subulate-lanceolate scales, and sporangia in discrete sori is arranged in a line on each side of midrib . In China, L. intermedium is mainly distributed in provinces Shanxi, Gansu, Hubei, Sichuan, and Guizhou . The whole plant of the fern is used in traditional Chinese medicine for inflammation, arthralgia, and bleeding stopping ). There exists taxonomic ambiguity for genera Lemmaphyllum, Weatherbya, Lepisorus, and Caobangia due to morphological similarity (Wang et al. 2010;Wei et al. 2017). The phylogenetic position of Lemmaphyllum and Lepidogrammitis remains in disputation (Wang et al. 2010). More importantly, the species delimitation in Lemmaphyllum is still an unresolved issue (Wei and Zhang 2013). Therefore, characterization of the whole chloroplast genome sequence of L. intermedium will be helpful to clarify the phylogeny and taxonomy of Lemmaphyllum.
Total genomic DNA was isolated from fresh and healthy fronds of L. intermedium collected from South China Botanical Garden, Chinese Academy of Sciences (23 11 0 3.56 00 N, 113 21 0 43.28 00 E). The specimen was deposited in the Herbarium of Sun Yat-sen University (SYS; voucher: SS Liu 20161024). We constructed a shotgun library with approximate insert lengths of 300 bp and performed a highthroughput sequencing on Hiseq 2500 platform (Illumina Inc., San Diego, CA). A total of 2.66 G pair-end raw data were generated. Trimmomatic v0.32 (Bolger et al. 2014) and FastQC v0.10.0 (Andrews 2010) were used for quality-trimming and assess. Then the obtained clean data were de novo assembled with Velvet v1.2.07 (Zerbino and Birney 2008). The assembled chloroplast genome was annotated by using GeSeq (Tillich et al. 2017) and tRNAscan-SE programs (Schattner et al. 2005), and corrected by blasting search against Lepisorus clathratus and Lemmaphyllum carnosum var. microphyllum (GenBank: KY419704 and MN623356) as references. To conduct phylogenetic analyses the complete chloroplast genome sequences of 12 ferns were retrieved from the National Center for Biotechnology Information (NCBI), and the sequence alignment was created by using the MAFFT v7.311 (Katoh and Standley 2013). A maximum likelihood (ML) tree was constructed with RAxML v8.2.12 (Stamatakis 2014) using Alsophila spinulosa as outgroup. Bootstrap values were computed from 1000 replicates.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Funding
This work was supported by the National Natural Science Foundation of China [32071781, 31670200, 31770587, and 31872670]

Data availability statement
The data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/nuccore/MT968973, GenBank accession number MT968973. Raw sequencing reads in this study are deposited in https://www.ncbi.nlm.nih.gov/sra/SRR12989517, with SRA number SRR12989517.