The complete chloroplast genome sequence of Ventilago leiocarpa Benth

Abstract Ventilago leiocarpa Benth. is an important medicinal and edible plant. The complete chloroplast genome of V. leiocarpa Benth. was assembled and annotated. In this study, the chloroplast genome of V. leiocarpa Benth. was a circular form of 161,880 bp in length. The genome presented a typical quadripartite structure consisting of a pair of inverted repeats (IRa and IRb) of 26,357 bp separated by a large single copy (LSC) region of 90,056 bp and a small single copy (SSC) region of 19,129 bp. The genome contained a set of 127 genes, including 82 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis showed that V. leiocarpa Benth. closely related to Rhamnus taquetii, which beyond to Rhamnaceae.

Ventilago leiocarpa Benth. belongs to the Rhamnaceae family. The sterm of V. leiocarpa Benth., 'Zi-jiu-niu' in Yao medicine of China, was offen used as an analgesic and for the treatment of cough, rheumatism, contused wounds, etc. (Lin et al. , 2001Zhou et al. 2001). V. leiocarpa Benth is rich in quinine compounds, such as emodin, physcion, ventilagolin, and ventiloquinone-I (Hu et al. 2020). The extract from the stem of V. leiocarpa Benth had anti-inflammatory and hepatoprotective properties Hu et al. 2020). It is widely used in Guangxi Jinxiu, China. However, the phylogenetic relationships of the V. leiocarpa Benth. have never been well tested. In this study, Illumina technology was applied to sequence, assemble and annotate the whole chloroplast genome of V. leiocarpa Benth.
The fresh leaves of V. leiocarpa Benth. were collected from Jinxiu Yao Autonomous County, Guangxi Province, China (N:24 11 0 5.74 00 , E:109 59 0 59.57) and the specimen was stored at Yao Medical Hospital of Jinxiu Yao Autonomous County Herbarium (ZJN202006). Five pieces of young leaves were used in this experiment. Total DNA was extracted by using a DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). The highquality DNA was sheared to the size of 300 bp for the shotgun library construction and the Genome was sequenced using Illumina Novaseq PE150 platform (Illumina Inc, San Diego), and 150 bp paired-end reads were generated.Lowquality reads and adapters were removed by the FastQC software (Andrews 2010). Paired end clean reads were combined and a total of 17,588,694 sequences were obtained for chloroplast genome assembly. Using the assembly method of Hahn et al. (Bernt et al. 2013), the reference sequence was selected from the published NCBI Berchemia berchemiifolia chloroplast genome (GenBank accession number: NC_037477). The annotation was mainly carried out by comparing the chloroplast genomes of related species, and the annotation results were confirmed and modified by CPGAVAS online tool (Zuo et al. 2017), and the reference genomes used for annotation is also B. berchemiifolia chloroplast genome (NC_037477). The annotated genomic sequence was registered into GenBank with an accession number (MT974496).
Rhamniaceae is a kind of dicotyledonous plants, including Rhamnaceae, Vitaceae and Leeaceae. To analyze the phylogenetic relationship of V. leiocarpa Benth., the complete chloroplast genome sequences of 10 Rhamniaceae family species from the NCBI GenBank database were downloaded. The phylogenetic tree was generated based on whole chloroplast genome sequences . The 11 complete chloroplast sequences were aligned by the MAFFT version 7.450 software (Katoh and Standley 2013). Phylogenetic analysis was conducted based on maximum likelihood (ML) analyses implemented in IQ-TREE 1.5.5 (Nguyen et al. 2015) under the TVM þ F þ R2 nucleotide substitution model (1000 bootstrap replicates), which was selected by ModelFinder (Kalyaanamoorthy et al. 2017;Ji et al. 2020).
The phylogenetic trees were analyzed with MEGA6 software (Koichiro et al. 2013) using maximum likelihood (ML) method (Bootstrap values were calculated out of 1000 replicates) (Yu et al. 2020). The phylogenetic analysis showed that V. leiocarpa Benth. closely related to Rhamnus taquetii, which beyond to Rhamnaceae. Our study will provide useful information on further clarifying the phylogenetic and evolutionary relationship in the Rhamnaceae (Figure 1).

Disclosure statement
No potential conflict of interest was reported by the author(s).

Funding
This work was supported by Initial capital for scientific research of highlevel talents in Guangxi Normal University of science and technology [GXKS2020GKY009].