The complete mitochondrial genomes of Parabotia kiangsiensis (Cypriniformes: Botiidae)

Abstract Parabotia kiangsiensis is an endemic species of genus Parabotia in China. In this study, we sequenced the complete mito-genome of P. kiangsiensis. The genome is 16,592 base pair (bp) in length, encoding 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and one non-coding control region (D-loop). The nucleotide composition is A: 30.66%, T: 25.13%, G: 16.3%, and C: 27.92% (AT content:55.79%). The complete mitogenome of P. kiangsiensis provides basic data for the genetic diversity conservation of this species.


Parabotia kiangsiensis; mitochondrial genome; phylogenetic analysis
Parabotia kiangsiensis (Cypriniformes, Botiidae, Parabotia), is a small-sized benthic fish endemic to China, mainly distributed in the Poyang basin (Liu and Guo 1986;Guo 2012). It is easy to distinguish P. kiangsiensis from other species of genus Parabotia, for having two extensive flaky lateral lobes and a free back edge in the lower lip (Liu and Guo 1986). Here, we first determined the complete mitochondrial genome of P. kiangsiensis and reconstructed the phylogenetic relationship with other Botiidae species. It may shed light on some genetic background of P. kiangsiensis, and could provide basic data for the genetic diversity conservation of this species.
In this study, the specimen of P. kiangsiensis were obtained from the Ganjiang River (115 10'E, 27 34'N), Jiangxi, China. Muscles were immediately fixed in 95% ethanol until it was picked out for DNA extraction. Some specimen of P. kiangsiensis were preserved in 10% formalin solutions and deposited in Nanchang Normal University (Voucher specimen: NCNU201912001).
Genomic DNA was extracted from muscle by using E.Z.N.A.V R Tissue DNA Kit (OMEGA, Beijing, China) following the manufacturer's instructions. DNA library preparation and 150-bp paired-end sequencing were performed on the Illumina HiSeq platform. After filtering, the mitochondrial data were assembled in NOVOPlasty version 4.0 (Dierckxsens et al. 2017). MITOS was used for annotating the newly sequenced mitogenome. Protein-coding genes (PCGs) and rRNAs were annotated by comparing with the published mitogenomes of the P. lijiangensis (Feng and Wang 2020).
The phylogenetic trees of the genera Parabotia and Leptobotia were reconstructed based on whole mitogenome dataset. Phylogenetic relationships of Parabotia and Leptobotia were constructed based on the multiple alignment of 22 mitochondrial genomes within the two genera (Saitoh et al. 2006;Li et al. 2012;Tian et al. 2014;Wan et al. 2014;Tian et al. 2015;Wei et al. 2016aWei et al. , 2016b and one outgroup Botia lohachata (Yu et al. 2016). ML analysis and NJ analysis were conducted using MEGA7 (Kumar et al. 2016) with 1000 bootstrap replicates. The phylogenetic tree strongly supported the close relationship of P. banarescui, P. lijiangensis, and P. Kiangsiensis (Figure 1). In the tree, P. banarescui and P. kiangsiensis formed a clade sister to P. lijiangensis, which was also congruent with the previous studies (Tang, Xiong, et al. 2005;Slechtov a et al. 2006;Tang, Liu, et al. 2006).

Disclosure statement
No potential conflict of interest was reported by the author(s).

Data availability statement
The data that support the findings of this study are openly available in Genbank with the accession codes MT850132 (https://www.ncbi.nlm.nih. gov/nuccore/MT850132).