Characterization of the complete chloroplast genome of Yuanjiang wild Ichang papeda (Citrus cavaleriei) in China

Abstract Ichang papeda (Citrus cavaleriei) is an endemic perennial plant in China. In this study, we assembled and annotated the complete chloroplast genome of Yuanjiang wild Ichang papeda using Illumina Hiseq-2500 sequencing data. The chloroplast genome is constituted of 160,996 bp, containing a 87,634 bp large single-copy region, a 18,762 bp small single-copy region, and a pair of 27,300 bp inverted repeat regions. The chloroplast genome contains 114 unique genes, including 80 protein-coding genes, 30 tRNAs and 4 rRNAs. Phylogenetic analysis showed that the relationship between the chloroplast gennomes of C. cavaleriei and C. reticulata is the closest, which consistently support their chloroplast relationships.

Citrus cavaleriei; wild Ichang papeda; chloroplast genome; phylogenetic analysis Ichang papeda (Citrus cavaleriei, once named as Citrus ichangensis), an original Citrus species with large wing leaves, is firstly discovered in Xingshan county, Hubei province of China (Chen et al. 2012;Yang et al. 2017). Nowadays, its distributions in Yunnan, Hunan, Guangxi, Guizhou, Sichuan, Chongqing and Shanxi provinces of China have also been reported. The Yunnan province of China, an important Citrus original center, is rich of endemic and wild Citrus species (Wu et al. 2018). And the wild Ichang papeda population in Yuanjiang county of Yunnan province is recognized the largest and the oldest (Chen et al. 2012). Current researches on Yuanjiang wild Ichang papeda were mainly focused on its genetic diversity and evolution from aspects of phenotype, stress resistance, molecular markers and so on (Chen et al. 2012;Yang et al. 2017). In the present study, to provide genetic information of Yuanjiang wild Ichang papeda, we assembled and annotated the complete chloroplast genome, and investigated its phylogenetic relationships with other Citrus species.
The specimen of Yuanjiang wild Ichang papeda was isolated from Mountain Nizubai, Yicibei village, Wadie town, Yuanjiang county, Yunnan province, China (23 29 0 33.036 00 N; 102 17 0 9.276 00 E) and samples were deposited at the Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Sciences. The leaf genomic DNA of Yuanjiang wild Ichang papeda was extracted using the CTAB method (Xie et al. 2020) and stored at the Institute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Sciences (No. YJYCC01). The whole genomic DNA re-sequencing was performed on the Illumina Hiseq-2500 platform to generate 125 bp pair-end reads (BIG, Shenzhen, CA, CHN). Totally, we obtained about 7 G high quality reads, which were aligned to chloroplast genomes of six Citrus species according to Wu et al. (2019) and Xu et al. (2019). Chloroplast genome assembly and annotation were performed according to the method described by Xu et al. (2019), and the annotated chloroplast genome has been deposited in Genbank with the accession number MT880606.
By using the complete chloroplast genomes of C. cavaleriei, 20 plant species from Rutaceae family and Ailanthus altissima (as outgroup), a maximum likelihood phylogenetic tree (Xu et al. 2019). Results showed that the relationship between the C. cavaleriei and C. reticulata chloroplasts is the closest (Figure 1), which consistently support their chloroplast relationship that has been put forward by many scientists (Nicolosi et al. 2000;Penjor et al. 2013;Yang et al. 2017).

Disclosure statement
No potential conflict of interest was reported by the authors.

Data availability statement
The raw data that support the findings of this study are openly available in NCBI Sequence Read Archive at [http://www.ncbi.nlm.nih.gov/bioproject/658640] under the BioProject ID PRJNA658640 and the annotated chloroplast genome has been deposited in Genbank [https://www.ncbi. nlm.nih.gov/genbank/] under the reference number MT880606. Figure 1. The maximum-likelihood phylogenetic tree constructed using the complete chloroplast genome sequences of Citrus cavaleriei and 20 plant species from the Rutaceae with Ailanthus altissima as outgroup. The phylogenetic tree was constructed according to the method and result described by Xu et al. (2019).