The complete chloroplast genome of Epimedium pudingense (Berberidaceae), a narrowly distributed plant species in China

Abstract Epimedium L. is the largest herbaceous genus of Berberidaceae which comprises more than 60 species. Epimedium pudingense is a rare plant species only narrowly inhabited in the Puding County in the Guizhou Province of China. Here, we first report the complete chloroplast genome of E. pudingense assembled from Illumina short-read sequencing data. The chloroplast genome of E. pudingense was 157,325 bp in length, with a total GC content of 36.11%. A total of 112 unique genes were identified, among which are 78 protein-coding genes, 30 tRNA genes and four rRNA genes. The phylogenetic analysis revealed that E. pudingense closely related to E. elachyphyllum. Our study will provide useful fundamental data for further phylogenetic and evolutionary studies of the Epimedium genus.

Epimedium L., the largest herbaceous genus of Berberidaceae, comprises more than 60 species discontinuously distributed in the temperate zone of Eurasia. (Zhang et al. 2014). China is the distribution and diversity center for possession of more than 50 species (mainly concentrate in the Hubei, Sichuan, and Guizhou Provinces). Due to their nourishing effect on kidney, muscles and bones, Epimedium plants have been used as traditional Chinese medicine "herba epimedii" for more than 2000 years. Modern pharmacological studies showed that herba epimedii has wide-reaching activities, including regulating bone remodeling, curing cardiovascular diseases, anti-tumor benefits and so on (Fan and Quan 2012).
Epimedium is one of the most taxonomically and phylogenetically intractable genus, since interspecific hybridization and gene introgression complicated the interspecific relationship. In recent years, the complete chloroplast genome has shown many great advantages in plant phylogenetic research, such as mass storage of information, relatively conserved gene content and genome structure, moderate nucleotide substitution rate and so on (Zhang and Li 2011). Considering how large and complex this genus is, there is still more species need to be sequenced and assembled to clarify the phylogenetic question.
Epimedium pudingense is a rare species only distributed in the Puding County of Guizhou Province in China, and it is used as herba epimedii by local people. Furthermore, E.
pudingense is a medicinal Epimedium germplasm resource peculiar to the Guizhou Province in China (He et al. 2011). In this study, we present the first complete chloroplast genome of E. pudingense. The results will provide more useful information for phylogenetic and evolutionary research of the genus Epimedium.
For this study, the E. pudingense was sampled from the Puding County of Guizhou province (China; 29 19 0 N, 105 44 0 E). A voucher specimen (Guo0348) was deposited at the Herbarium of the Institute of Medicinal Plant (IMPLAD), Beijing, China. Total genomic DNA was extracted from the fresh leaves of E. pudingense using the modified CTAB method (Doyle and Doyle 1987). The high-quality DNA was sheared to the size of 300 bp for the shotgun library construction. Genome sequencing was performed using Illumina Novaseq PE150 platform (Illumina Inc, San Diego), and 150 bp paired-end reads were generated. We assembled the chloroplast genome using the program GetOrganelle version 1.6.2 (Jin et al. 2018) with E. acuminatum chloroplast genome (GenBank accession number: NC_029941) as a reference. The annotation of chloroplast genome was conducted through the online program CPGAVAS 2 (Shi et al. 2019), and assisted with careful manual correction. The annotated genomic sequence was registered into GenBank with an accession number (MT506232).
To identify the phylogenetic position of E. pudingense, we downloaded the complete chloroplast genome sequences of 16 species from the NCBI GenBank database. MAFFT v7 (Katoh et al. 2017) was used for sequence alignment, and then a maximum likelihood tree was constructed by using RAxML v8.2.10 (Stamatakis 2014) with Clematis terniflora as the outgroup (Figure 1). The phylogenetic analysis revealed that E. pudingense closely clustered with E. elachyphyllum. Our study will provide essential data for future research on the phylogenetic and evolutionary relationship in Epimedium genus.

Disclosure statement
No potential conflict of interest was reported by the authors.