The complete mitochondrial genome of the firefly, Pteroptyx maipo (Coleoptera: Lampyridae)

Abstract We report the complete mitochondrial genome of firefly, Pteroptyx maipo. The circular genome of 16,127 bp has a base composition of A (44.22%), C (11.60%), G (8.23%) and T (35.96%). Our sequence is similar to other Metazoa, which contains 13 protein-coding genes. All 13 protein-coding genes were initiated by the ATN (ATT, ATA and ATG) codon. Nine protein-coding genes stopped with TAA or TAG codon and the other four genes have an incomplete termination codon, a single T. We sequenced the mitochondrial genome of fireflies to analyze phylogenetic relationships and deduce the evolution of their flashing signals.


Introduction
Firefly are a family of insects in the beetle order Coleoptera. Using morphological characters, 30 species are recognised in the genus Pteroptyx following revisions by Ballantyne and McLean (Ballantyne and McLean 1970;Ballantyne 1987aBallantyne , 1987bBallantyne , 2001. Some Pteroptyx species have spectacular synchronous flashing behavior (Lloyd 1973;Case 1980;Moiseff 1997, 2004;Jusoh et al. 2013). Pteroptyx maipo Ballantyne et al. (2011) is the first species of Pteroptyx firefly recorded in China and distributed in Hong Kong (Ballantyne et al. 2011). Pteroptyx maipo flash like other Luciolinae fireflies but not in synchrony (Ballantyne et al. 2011).
Mitochondrial genome sequences are essential to a comprehensive understanding of the evolution of Lampyridae and other luminescent beetles (Ermakov et al. 2006). Here, we elucidate the mtDNA genome of P. maipo.
These male fireflies used in this study were collected from Enping city, Guangdong Province, China (22 01 0 N, 112 21 0 E) in 13 July 2013, and were stored in Natural History Museum, Huazhong Agricultural University, Wuhan, Hubei, China (its accession no. PM2011052301). Pteroptyx maipo's habits, flashing signals and some morphology have been studied in detail (Ballantyne et al. 2011). However, there is no genetic research information about P. maipo.
Specific primers were designed based on these conserved regions sequences. The PCR reaction was carried out with LA Taq polymerase for 35 cycles at 94 C for 30 s, and annealed at 50 C for 30 s, followed by extension at 72 C for 1 min per 1 kb. Sequences were assembled using the software DNAstar v7.1 (DNAstar, Madison, WI) and adjusted manually to generate the complete sequence of mitochondrial DNA.
The phylogenetic tree among the five species based on mitochondrial genome sequences were aligned in MEGA 5 (Phoenix, AZ) (with 1000 bootstrap replicates) to construct a Neighbour-Joining tree (Figure 1).
The result shows P. maipo is most closely related to Luciola Substriata (recently identified as Sclerotia flavida by Ballantyne et al. 2016), which belongs to an entirely different genus in the Lampyridae.
In conclusion, the complete mitochondrial genome sequence of P. maipo provides an important molecular framework for further phylogenetic analyses of fireflies. These data are essential for comprehensive understanding of the role of sexual and natural selection in the evolution of firefly flashing signals.

Disclosure statement
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the article.

Funding
This research was supported by the Natural Science Foundation of China [No. 31372252 and No. 31672349].