Complete mitogenome of Intermediate Egret Ardea intermedia (Ciconiiformes: Ardeidae)

Abstract The Intermediate Egret Ardea intermedia belongs to family Ardeidae, and it is widely distributed over east Africa across the Indian subcontinent to Southeast Asia and Australia. In the present study, the total mitochondrial genome of A. intermedia was determined. The genome is 18,578 bases in length and contains 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions (CR and CRR), with a base composition of A 30.8%, G 14.0%, T 24.3% and C 30.9%. The dn/ds values of ten PCGs (ND1, ND2, ATP8, ATP6, Cox3, ND3, ND4L, ND4, ND5 and ND6) are below 1. Bayesian inference (BI) and maximum likelihood (ML) methods generated similar topologies. Phylogenies showed that Ardea novaehollandiae and A. intermedia should be assign to Egretta and Ardea, respectively. The mitogenomic data of A. intermedia will be useful in the conservation genetics and phylogeny of the species.

The Intermediate Egret Ardea intermedia is a medium-sized white egret in the family Ardeidae. It is a resident breeder from east Africa across the Indian subcontinent to Southeast Asia and Australia. The wild population is decreasing and the species has been evaluated as Least Concern by IUCN (IUCN 2017). So far, limited molecular data on the species have been reported (Jansen et al. 2009;Zhou et al. 2014). Here, we sequenced and characterized the complete mitogenome of A. intermedia, with the individual (JAF001) captured in Yongxiu (115.888 o E,29.067 o N), Jiangxi Province and which was preserved in Jiangxi Academy of Forestry, to provide basis data for conservation genetics and evolution of this species.
Total genomic DNA was extracted from tissue samples using standard phenol/chloroform methods (Sambrook and Russell 2001). The PCR protocol followed the study of Zhou et al. (2014) and made several modifications.
In the present study, the complete mitogenome of A. intermedia (GenBank Accession Number KX592585, 18,578 bp), is comprised of 13 protein-coding genes (PCGs), 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions (CR and CCR) ( Table S1). The gene order of A. intermedia was identical to that observed in the most birds (Zhou et al. 2014;Huang et al. 2016Huang et al. , 2017. The total base composition of the mitochondrial genome is showed as follows: A 30.8%, G 14. 0%, T 24.3% and C 30.9%, with an A þ T-rich pattern (Zhou et al. 2014).
We also retrieved 13 PCGs of 26 species. Bayesian inference (BI) and maximum likelihood (ML) methods were performed based on concatenated 13 PCGs. BI analysis was carried out as mentioned by Tu et al. (2012). ML was performed using PhyML 3.0 online platform (Guindon et al. 2010) with default parameters. Phylogenies based on BI and ML showed similar topologies (Figure 1). The phylogeny ((Ardeidae, Threskiornithidae) Ciconiidae) was highly supported with the BI analysis. A. novaehollandiae clustered with Clade B rather than Clade A, and thus A. novaehollandiae should be assigned to the genus Egretta. The results were consistent with previous studies (Chen et al. 2000;Zheng 2011). Ardea modesta was sister relationship to A. intermedia.
Until now, the generic placement of A. intermedia has been disputed. The species was placed in the genus Mesophoyx (MacKinnon et al. 2000), while some authorities (Chen et al. 2000;Zheng 2011) listed it in genus Egretta. In this study, the species should be considered in the genus Ardea and the results were in concordant with Zhou et al. (2014). Complete mitochondrial genome sequences within the family Ardeidae are still deficient and the molecular data may contribute to the conservation genetics and the phylogenetic evolution position of the species.