Development of gelatin-carboxymethylcellulose coatings incorporated with avocado epicarp and coconut endocarp extracts to control fungal growth in strawberries for shelf-life extension

ABSTRACT The objective of this study was to incorporate avocado peel (AEE) and coconut (CEE) extracts as antifungal additive in the development of gelatin and carboxymethylcellulose coatings to extend shelf life in strawberries. Fractions of 200 mg/L of extracts were incorporated in the coating and characterized during storage at 2°C. AEE and CEE in the coating delayed significantly (P < .05) weight loss (40%), softening, color, and compositional changes (°Brix, sugars, titratable acidity). Rhizopus stolonifer and Aspergillus niger strains were isolated and inoculated to evaluate the effectiveness of the coatings on strawberries. The use of the coating + plant extracts decreased the proliferation of fungi by 45% compared to the uncoated fruit and extended the shelf life for 15 days. With these findings, it is suggested that biopolymeric coatings + plant extracts can be used as a potential method of ecological preservation in strawberries against microbial deterioration.


Introduction
Strawberries (Fragaria x ananassa) are a non-climacteric fruit that must be harvested with full maturity to achieve excellent nutritional composition. However, due to its accelerated metabolism and postharvest activities, it can have a reduced shelf life (Q. Li et al., 2020). The softness and turgor of the fruit walls leave it exposed to mechanical injuries, softening, and even more deterioration due to fungal rot in the postharvest period (Muley & Singhal, 2020). Fungal disease caused by fungi Botrytis cinerea, Rhizopus stolonifer, Aspergillus niger, and Colletotrichum acutatum are the most infectious and harmful in strawberry postharvest management (Swett et al., 2020;Ventura-Aguilar et al., 2018). Consequently, fruit rotting is an economic vulnerability even in well-managed fields. This can lead to economic losses that exceed up to 50% of the total harvest worldwide. For this reason, the difficulty of preserving the fruit and reducing shelf life has led to the commercialization of the strawberry in frozen form or processed as jam or jelly. However, this processing reduces nutritional properties and the possibility of fresh consumption of strawberries (Octavia & Choo, 2017).
Due to the nutritional and economic importance of strawberries, different preservation techniques have been studied to reduce losses and extend the commercial fruit life. Precooling and refrigeration are widely used to preserve quality and ensure food safety and innocuousness (Zhao et al., 2019). Likewise, modified and controlled atmospheres can manage strawberries through commercialization chains (Anami et al., 2020). Other techniques such as radiation (Shankar et al., 2021), high-temperature and sonication (Yildiz & Aadil, 2020), ultraviolet light (Janisiewicz et al., 2021), electromagnetic fields (Giannoglou et al., 2021), osmotic treatments , and polymeric coatings (Abdollahzadeh et al., 2021) have been evaluated for food preservation. However, the application of these treatments at an industrial level has been restricted by external environmental factors such as relative humidity and temperature changes (Bovi et al., 2018). Thus, biopolymeric active coatings have received particular attention due to their biodegradability and fruit affinity characteristics. Coatings can be formed individually or combined with different biopolymers, waxes, mucilages, and other components related to food. Thereby, gelatin-based coatings have been extensively used and receive special attention due to their distinctive functional properties as coating-forming capacity, gel formation, good oxygen barrier, and considerably improving the sensory characteristics of food (Cardoso et al., 2016;Kingwascharapong et al., 2020;López-Palestina et al., 2019;Xiong et al., 2021). Likewise, gelatin coatings are water-soluble, non-toxic, biodegradable, low cost, and biocompatible with lipids and polysaccharides (Gómez-Guillén et al., 2011;Ramos et al., 2016). However, gelatin's limited thermal stability and mechanical properties reduce its potential application (Etxabide et al., 2017;Luo et al., 2022). These properties have been enhanced when interacting with polymers such as casein (Khedri et al., 2021;Pellá et al., 2020), chitosan (Hajji et al., 2021;Nagarajan et al., 2021), starch (Friedrich et al., 2020;Pattanayaiying et al., 2019), Persian gum (Khorram et al., 2017;Sharif et al., 2021), alginate (Abdallah et al., 2018;De Souza et al., 2021), and carboxymethylcellulose (Salama et al., 2019;Samsi et al., 2019).
Carboxymethylcellulose (CMC) and gelatin (Gel) represent an ecological, compatible, and a highly biodegradable alternative as an edible coating for food preservation. The combination of CMC and gelatin has been reported to have a range of benefits like increased viscoelasticity, resistance to oil/fat, enhanced coating-forming properties, ability to extend the shelf-life of fruit and vegetables. Additionally, Gel/CMC biopolymeric matrix can form transparent edible coatings-films and could be a carrier for active additives as antioxidants and antimicrobial agents (Esteghlal et al., 2018;Mu et al., 2012;Panahirad et al., 2021).
Furthermore, biopolymeric active coatings are useful as vehicles to transport food additives, natural antimicrobials, antioxidants, pigments, essential oils, and natural extracts to avoid the deterioration of the fruit and extend the shelf life (Muley & Singhal, 2020). Plant extracts with the potential for microbial inhibition have been suggested to lengthen shelf life by controlling fungal development. In this regard, the application of essential oils (EO´s) in strawberry fruit such as those obtained from cinnamon (Cinnamomum zeylanicum; Chu et al., 2020) and clove (Syzygium aromaticum; Hasheminejad & Khodaiyan, 2020) can inhibit the growth of various fungal species such as Penicillium expansum (Da Rocha Neto et al., 2019), B. cinerea (Campos-Requena et al., 2017), and R. stolonifer (Oliveira et al., 2019). However, EO´s can alter the sensory properties of the fruit although the inhibition may only be temporary due to EO´s volatile nature. Thus, alternatives are needed that do not alter the sensory attributes and that do not lose their antimicrobial effect by volatilization. Consequently, other antifungal materials have been tested such as olive leaf extracts (Khemakhem et al., 2018), pecan nut (Yemmireddy et al., 2020), banana peel , coconut shell (Prakash et al., 2018), and avocado peel (Melgar et al., 2018).
Avocado and coconut processing has increased for obtaining products like guacamole, avocado pulp, coconut flour, coconut sugar, coconut cheese, cosmetic products like face creams, and oils generating a high content of peels and seeds residues (Del Castillo-Llamosas et al., 2021). The peels represent 7-24% of the dry weight of the fruit, which commonly does not have industrial use despite the high content of bioactive compounds (Adilah et al., 2018). Furthermore, avocado peels are known for their antioxidant and antimicrobial properties. It contains a high content of compounds, including flavonoids, phenolic acids, organic acids, lignans, and fatty acids (Ramos-Aguilar et al., 2021;Salazar-López et al., 2020). The coconut shells and husks represent a source of fiber and many phenolic compounds (Tanwar et al., 2021). The phenolic compounds in coconut endocarp are due to the presence of quercetin, kaempferol, chlorogenic acid, catechin, and epicatechin (N. Li et al., 2021). Several authors indicated that phenolic compounds have antioxidant, antiradical, antibacterial, and antifungal activity (Christ-Ribeiro et al., 2019;Liu et al., 2021;Mekam et al., 2019;Rashed et al., 2014;Sagdic et al., 2011).
However, the avocado epicarp and coconut endocarp extracts have not been incorporated in a biopolymeric matrix as an alternative for the conservation of strawberry fruit against phytopathogens. Thus, the objective of this work was to develop gelatin-carboxymethylcellulose coatings incorporated with extracts of avocado epicarp and coconut endocarp applied on strawberry fruit to extend their shelf life. Moreover, to determine their application were investigated; a) the postharvest behavior of coated strawberry through compositional changes during storage, and b) the effects of coatings on controlling fungal development in strawberry fruit using R. stolonifer and A. niger strains.

Obtaining plant extracts
Avocado (Persea americana Mill.) and coconut (Cocos nucifera) fruit were collected from a local market in Texcoco, Mexico at consumption maturity. Avocado epicarp extract (AEE) and coconut endocarp extract (CEE) were obtained according to Aguilar-Méndez et al. (2020), with some modifications. The avocado epicarp and coconut endocarp were dried separately in a digital oven (Fe-294AD Feligneo, México) at 40 ± 2°C. The dried product was pulverized in a disc mill (148-2, The Bauer Co., USA) and sieved through 250 μm stainless steel mesh (Montinox, Montiel Inoxidables, Mexico). Dry samples of 12.5 g avocado epicarp and 15 g coconut endocarp were weighed and placed in a 500 mL mixture of water:ethanol at ratios of 7:3, 3:7, respectively. After sonication in water for 60 min (TI-H-5, Elma, Germany), at a frequency of 45 kHz, during at 65°C. Then, the samples were centrifuged at 1750 rpm (K International, Waukegan, USA), for 15 min and filtered on Whatman paper No. 3 (Merck, Kenilworth, NJ, USA). The extracts were concentrated on a rotary evaporator at 45°C for 4 h (RE-500; Yamato Japan), and were lyophilized (FreeZone 4.5 Freeze Dry System, Labconco, USA) and stored in an amber flask until use.

Preparation of biopolymer solutions
A solution consisting of type B gelatin, CMC (both purchased) from Sigma-Aldrich (USA), and glycerol (Merk Inc., Germany) each at 0.5% (w/v) was prepared using 125 mL water with stirring at 90 ± 2°C at 600 rpm for 30 min on a grill (Thermlyne, lowa, USA). Three solutions were prepared: The first was based on gelatin (Gel) and CMC (Gel/ CMC) and was kept without any additional additives; the second had AEE added at 200 mg/L (Gel/CMC/AEE); and the third had CEE added at 200 mg/L concentration (Gel/CMC/CEE).

Application of polymeric solutions in fruit
Fresh strawberries were purchased from a local market in Texcoco, Mexico. Strawberries of uniform size, shape and colour, without any signs of mechanical damage or fungal decay, were selected for this study. The strawberries were washed with water, sanitized by immersion in a 1% (w/w) sodium hypochlorite solution for 1 min, and rinsed with distilled water. Four batches of strawberries were created to study the solutions on the fruit, the first one was handled with any treatment and labelled as no covering (NC) and the three ones were treated with biopolymers: Gel/CMC, Gel/ CMC/AEE, and Gel/CMC/CEE. The polymer solutions were applied to the fruit with an immersion method and exposure to airflow from a fan (OBF851-LA013, Oster, China) operating at 300 rpm for 20 min to dry the surface at room temperature. The material from each group was organized in batches of 15 fruit. These were placed at 2 ± 1°C and 93% relative humidity in open polyethylene terephthalate containers with a capacity of 1 L. Each treatment was performed in triplicate, and the evaluations were performed on 0, 3, 6, 9, 12, and 15 days of storage.

Weight loss
All treatments were weighed at the beginning (0 d) and upon removal from storage with an analytical balance (Ax224, Ohaus, Taiwan) with a precision of 0.0001 g. According to the initial weight, the cumulative weight loss in percentage was determined based on an AOAC (1994).

Firmness
The firmness was determined in three points of the fruit with a texture analyzer TA-XT2i (Stable Micro Systems, England). The firmness was expressed in Newtons (N) as the average of all the measurements.

Total soluble solids (°Brix), reducing sugars, and titratable acidity (TA)
Total soluble solids (TSS) were expressed as percentages and measured with a refractometer (LH-T50, Lohand Biological, China) in a drop of fruit juice. Reducing sugar content was measured by the Lane-Eynon method using the procedure described by AOAC (1998). Titratable acidity (TA) was determined by taking 5 mL samples of juice and titrated with 0.1 N NaOH using phenolphthalein as an indicator. The results were expressed as a percentage of citric acid.

Color index
Color measurements were recorded using a high-resolution colorimeter (NH300, China) for each treatment and storage time. Values of a* (greenness/redness) and b* (blue/yellowish) were recorded to calculate chromaticity (C*) and tone angle (Hue) according to the following Equations (1) and (2): A blank was taken as standard L* = 94.512, a* = 0.417, and b* = 0.350.

Fungal growth analysis 2.3.5.1. Molds. R. stolonifer and
A. niger were isolated from strawberry fruit and placed in potato-dextrose-agar (PDA) culture medium at 24°C. An aqueous 0.5% Tween 80 solution was prepared from which 9 mL were taken and mixed with 1 mL inoculum of each strain. Successive dilutions were made in the range 10 2 to 10 7 spores/mL. The solutions were adjusted with 0.5% Tween-80 at 10 6 spores/mL.

Strawberry inoculation and coating. Strawberry
inoculation and coating were performed as reported by Park et al. (2005) with some modifications. Five fruits were used per treatment. These were free of defects and had a uniform color. The samples were disinfected with a 1% sodium hypochlorite solution for 1 min with two rinses with sterile water followed by a forced air dry (OBF851-LA013, Oster, China) at 300 rpm for 20 min at room temperature. Inoculations were performed by spraying 0.5 mL of 10 6 mL/spore of R. stolonifer or A. niger solution and subsequent drying at room temperature for 20 min. The strawberries were then immersed in coating solutions (NC, Gel/CMC, Gel/CMC/AEE, Gel/CMC/CEE) for 60s under a hood and with forced air again as shown in Figure 1.

Statistical analysis
Evaluation of compositional changes was performed as a 4 × 6 factorial arrangement in a completely randomized design. One factor was the treatment to which the fruit were subjected (NC, Gel/CMC, Gel/CMC/AEE, Gel/CMC/CEE). The other was time with six levels (days of storage). The evaluation phase of the fungal growth of extracts was carried out as a 2 × 4 factorial arrangement housed in a completely randomized design where one of the factors was inoculation (fruit inoculated with R. stolonifer and A. niger) and the other variable was type of coating applied. Analysis of variance and comparison of treatment means were conducted with Tukey's test with a significance level of 0.05. All evaluations were performed in triplicate and analysis was performed using Minitab 7.0 program.

Weight loss
The fruit of all treatments experienced significant and continuous weight loss during storage (P < .05), as shown in Figure 2a. The greatest loss occurred in NC fruit at a rate of 15.71% d −1 , which contrasted with the rates of 7.22%, 2.73%, and 3.49% d −1 observed in the fruit of Gel/CMC, Gel/CMC/ AEE, and Gel/CMC/CEE, respectively, with a significant difference among groups. At the end of each storage, the weight loss was 94.7% in NC on day 6, 84.7% in Gel/CMC on day 12, 39.1% in Gel/CMC/AEE on day 15, and 52.2% in Gel/CMC/CEE on day 15. Coating with AEE was more effective in reducing weight loss than the covering incorporated with CEE. This difference between extracts could be since the avocado peel has a greater number of phenolic compounds, in addition to a natural antifungal compound called persin (Bowen et al., 2018;Salazar-López et al., 2020). Weight loss increased considerably as storage time increases, and as expected, the greatest weight loss occurred in untreated fruit (NC). Conversely, a reduction in weight loss was observed in the Gel/CMC treatment as in the strawberries coated with AEE and CEE. This reduction in weight loss in the fruit with coatings could be caused by the formation of a semipermeable and protective layer that could lead to a lower respiration rate. Consequently, this led to a lower production of CO 2 and delayed water vapor transfer thus prolonging the commercial life of strawberries by 5 days. The permeability and the barrier against gases (O 2 and CO 2 ) in biopolymeric coatings depend directly on storage conditions such as relative humidity and temperature (Hernández-Muñoz et al., 2008). Physiological weight loss is a common phenomenon in fruit caused mainly by transpiration (evaporation of moisture through the skin) due to a vapor pressure deficit (dependent on the gradient between the fruit tissue/atmosphere/temperature; Hübert & Lang, 2012). Therefore, incorporating AEE and CEE in the hydrophilic biopolymeric matrix reduces the permeability to water leading to less water loss during storage. This is demonstrated by the reduction in weight loss in coated fruit versus NC fruit. Muley and Singhal (2020) observed the same trend in strawberries covered with a layer of chitosan and isolated whey protein. This phenomenon correlated a gradual  weight loss with storage time as a function of moisture loss and respiration in strawberries.
Another factor that promotes weight loss is fungal development. Microorganisms accelerate the glycolysis process, which leads to a loss of carbohydrate reserves in the fruit (Sogvar et al., 2016). Additionally, fungal infections in NC and Gel/CMC batches leads to a physiological imbalance that quickly results in natural senescence of the fruit, thus causing a change in color (cell necrosis), tissue degradation, and the appearance of physical lesions associated with the colonization of microorganisms. These microorganisms induce morphological changes, structural alterations, and disorganization at the molecular level (Valenzuela et al., 2015). Consequently, Ventura-Aguilar et al. (2018) demonstrated that the conservation of strawberries with Hibiscus sabdariffa extracts in combination with the essential oil of cinnamon and chitosan on the growth of Colletotrichum fragariae sustains high effectiveness of natural extracts for microbial control. In summary, the incorporation of AEE and CEE in the matrix Gel/CMC, decreased the water vapor transfer in the fruit and significantly reduced the fungal development thus slowing glycolysis and preserving the integrity of tissues and allowing less loss of accumulated weight.

Firmness
Continuous loss of fruit firmness also occurred in all treatments with an average rate of 0.41, 0.19, 0.09, and 0.10 N d −1 in NC, Gel/CMC, Gel/CMC/AEE, and Gel/CMC/CEE, respectively; the difference was significant among all treatments (Figure 2b). The initial firmness was 2.68 N, and softening occurred up to 0.25 N in NC, 0.23 N in Gel/CMC, 1.33 in Gel/ CMC/AEE, and 1.15 in Gel/CMC/CEE at the end of the storage. The NC fruit could only be handled for 6 days in storage. At that time, they had lost 90.5% of their consistency, which contrasted with the fruit handled with the Gel/ CMC coating that had firmness reduced by 50.7%. Those treated with Gel/CMC/AEE and Gel/CMC/CEE had firmness reduced by 20.4 and 30.9% on day 6, respectively. After 15 days of storage, the fruit treated with Gel/CMC/AEE and Gel/CMC/CEE presented 50.7 and 57.0% loss of consistency in their tissues, which was less than what occurred in the fruit of the Gel/CMC treatment at 12 days of storage. These results indicate that the most effective treatment to maintain firmness was the one that incorporated AEE followed by the one that used CEE followed by the one that was based only on the Gel/CMC coating. These results could be since the plant extracts reduced the proliferation of fungi. Likewise, they kept the walls of the plant tissue of the strawberries stable and without deterioration. Similarly, Nasrin et al. (2017) pointed out a reduction of firmness effect in strawberries coated with aloe vera.
The change in firmness is generally associated with the deterioration rate that indicates the loss of consistency of the fruit tissues and favors microbial development. Softening is a phenomenon that generally accompanies the ripening of fruit, but it develops even in the senescence phase due to degradation by depolymerization of cell walls (Posé et al., 2019;Prasanna et al., 2007). In particular, the breaking of the pectin chains is a critical factor in the changes in the texture of strawberry fruit (Wang et al., 2018).
In addition to firmness, another essential characteristic that determines the postharvest handling period is fungal development. The cell wall constitutes a barrier for the access of fungal species. Furthermore, the cell wall allows the release of oligalacturonide fragments with a degree of polymerization between 10 and 15 units of the pectin chains that exert an antimicrobial defense activity (Osorio et al., 2008). However, in the presence of pectin, fungal species such as A. niger favor the production of enzymes that cause the breaking of the pectin chains into galacturonic acid units that in turn lead to degradation of the cell wall and favor greater fungal development (Q. Li et al., 2020). Therefore, the presence of the antimicrobial properties of the natural extracts and the development of a physical barrier through the biopolymer matrix delayed softening and resulted in a longer shelf life.
These results confirm that the Gel/CMC coatings incorporated with AEE and CEE could improve the preservation  characteristics of the fruit more effectively than the fruit that only have the Gel/CMC coating and NC fruit through two mechanisms as mentioned earlier: 1) The retardation of the process of rot prevents the development of fungal species in strawberries; and 2) the reduction in the rate of degradation of cell walls due to the formation of a physical barrier, which makes pectin inaccessible for its hydrolysis by the action of fungal species.

Total soluble solids (°Brix), reducing sugars and titratable acidity (TA)
The initial TSS content was 7.99 °Brix. During storage, the TSS increased in the fruit for all treatments (Table 1). After 3 days, the content was similar across all groups with a value of 8.20 °Brix. Subsequently, in fruit treated with Gel/CMC, the TSS value increased to 9.31 °Brix, and the contrast was significant among fruit of different treatments. At the end of storage, the TSS content was 8.97 °Brix in Gel/CMC (day 9), 9.10 °Brix in Gel/CMC/AEE (day 15), and 8.55 °Brix in Gel/ CMC/CEE (day 15). That is, the fruit with coatings incorporated with AEE followed by the CEE coatings were those that reached the lowest levels of TSS versus NC fruit.
The TSS content is mainly determined by the presence of sugars (Nunes et al., 2006). Thus, the reducing sugar content follows the trend observed in the TSS results. The reducing sugar content of the fruit at the beginning of storage was 3.48%, and the behavior of the treatment (NC) group showed a moderate increase in these compounds to 3.7% on day 3 and then a significant increase to 4.86% on day 6. The Gel/CMC, Gel/CMC/AEE, and Gel/CMC/CEE groups had a decrease in the content of reducing sugars between the beginning and 3 days to 3.04% with no difference between them. There was then a continuous increase to 4.50%, 4.55%, and 4.94%, respectively, at the end of each storage (Table 1). These results show that incorporating AEE and CEE in the Gel/CMC matrix modifies the consumption of sugars in the fruit versus NC fruit. This change can be observed in two stages. In the first stage, the fruit coated with Gel/CMC had an increase in reducing sugar consumption in the initial storage phase versus NC fruit. As a result of the application of the biopolymers, the barrier properties formed by the external tissues were modified including the cuticle and the biopolymeric gas layer of the fruit shell (De Medeiros et al., 2012). This modification of the barrier properties limited the supply of oxygen, and consequently, the supply of energy for the metabolic functions had to be evaluated with ATP obtained from oxidative phosphorylation. Finally, to address the deficiency through this metabolic process, it is necessary to increase the energy obtained from glycolysis for which the consumption of sugars as a substrate had to be increased. Therefore, a higher consumption of sugars will be observed in coated fruit as a substrate of the respiratory process (Chu et al., 2020). These results were similar to Quintana et al. (2021) in strawberries covered with chitosan and natural extracts/essential oils from different plants. The increase in the TSS was due to the metabolic process (converting starch and acids into sugar) during postharvest storage.
In the second stage starting from day 3 of storage, an increase was observed in the content of reducing sugars in the fruit treated with Gel/CMC, Gel/CMC/AEE, and Gel/CMC/ CEE. This trend was caused by the solubilization of compounds and the breakdown of polymers of the cell walls. However, the TSS values in coated fruit reached their maximum at a lower rate at the end of storage. This behavior occurs because the coatings can reduce the respiration of the fruit and delay its ripening. Thus, the permeability of CO 2 and O 2 can be controlled by the coatings and slow down the ripening of the fruit because both gases are produced due to the degradation of sugars (Pinzon et al., 2020;Robles-Flores et al., 2018). Dong and Wang (2017) have reported similar findings on coatings of CMC and garlic EO´s to preserve strawberries. The study pointed out that the gradual change in TSS content could be due to the deceleration of metabolic activity. Consequently, as mentioned above, a decrease in respiration, likewise, the loss of water in the fruit could cause changes in the TSS content and, at the same time, less fungal growth due to the lack of glucose.
On the other hand, fruit of all treatments had a titratable acidity (TA) of 0.99% at the beginning of storage, and this parameter decreased up to 0.56% in NC after storage of day 6, 0.61% in Gel/CMC at the end of storage of day 9, 0.59% in Gel/CMC/AEE, and 0.45% in Gel/CMC/CEE upon completion of the day 15 storage with modification rates of 0.093, 0.067, 0.039 and 0.033% d −1 , respectively (Table 1). In general, the fruit treated with Gel/CMC/AEE and Gel/CMC/CEE were similar in most of the storage, except at the end when a significant difference was observed in the latter. The coated fruit with Gel/CMC/AEE and Gel/CMC/CEE reduced the rate of consumption of organic acids. The metabolism modification affects the fruit acidity. Perdones et al. (2012) have also reported a decrease in TA values attributed to over-ripening and rotting in strawberries treated with a chitosan matrix with lemon EO's. Therefore, the acidity decreases due to the consumption of organic Table 1. Soluble solids, reducing sugars, titratable acidity of no covering (NC) and coated strawberries (Gel/CMC, Gel/CMC/AEE and Gel/CMC/CEE) stored at 2°C. Tabla 1. Sólidos solubles, azúcares reductores, acidez titulable de fresas no recubiertas (NC) y recubiertas (Gel/CMC, Gel/CMC/AEE y Gel/CMC/CEE) almacenadas a 2°C.
acids during respiration at storage (Riaz et al., 2021). According to Nunes et al. (2006), the increase in TSS and the decrease in titratable acidity are associated with ripening. However, once this physiological process is completed, the modification of TSS is negligible and is attributed to the solubilization of polymers of the cell wall, which is consistent with the changes seen for firmness. These authors also showed that acidity could be expressed as a percentage of citric acid because it has the highest presence in strawberry fruit, and the content can decrease slightly or remain unchanged during postharvest storage. In conclusion, as mentioned earlier, the Gel/CMC coating incorporated with AEE and CEE could modify the barrier properties in consecutive stages: i) they increase the consumption of sugars in the short term, and ii) they reduce the permeability of gases and slow down the maturation and the consumption of organic acids. Thus, AEE and CEE incorporated in Gel/CMC coatings slow down the ripening of strawberries and, consequently reduce compositional changes.

Index color
Color undergoes some remarkable changes as fruit ages. It markedly impacts consumer acceptance. Figure 3a-b shows the color changes of the fruit with different treatments during storage (2°C). These values are denoted as colorimetric changes measured directly from the fruit tissue: chroma and tone angle. Chroma results are shown in Figure 3a. Significant changes (P < .05) in the chromatic value were observed between the fruit treated with NC and Gel/CMC compared to those incorporated with plant extracts on 3 and 6 days. However, throughout storage, all treatments exhibited coloration with less intensity (less vivid) as indicated by the chroma values, although the highest values were from the Gel/CMC/AEE and Gel/CMC/CEE treatments at the end of the study without significant differences between them. The chroma was reduced for Gel/CMC by around 33%. It was 20-25% for Gel/CMC/AEE and Gel/CMC/CEE treatments. The change in coloration is mainly due to the loss of water (transpiration). This phenomenon quickly leads to tissue degradation, causing an alteration of color in the fruit (senescence; Perdones et al., 2012). Therefore, coatings with plant extracts significantly decreased the rate of color change by avoiding tissue degradation.
The tone angle values are shown in Figure 3b. There were significant differences between the NC treatment and the rest of the treatments during storage. The change in hue, darkening, and oxidation of the cuticle was perceived on a different scale in all treatments. These changes may be symptoms of excessive maturity and depolymerization of tissues that occur during transpiration (dehydration) and senescence caused by the loss of intracellular fluids and tissue collapse (cell death; Hernández-Muñoz et al., 2008;Velickova et al., 2013). In addition, Soazo et al. (2015) have found similar behavior in color variability and suggest that this phenomenon is due to the content of anthocyanins, which are not chemically stable in strawberries coated with whey protein-based matrix.
Consequently, as mentioned before, the coatings incorporated with AEE and CEE could protect the fruit from cuticle oxidation caused by excessive maturity by reducing transpiration and tissue collapse. These materials offer better coloration with only minor darkening.

Fungal growth analysis
Fungal development was observed and limited the handling of NC materials to only 6 days. Although the Gel/CMC treatment reduced microbial development, its progress indicated that the useful life of the fruit ended on day 9. This behavior contrasted with the materials treated with AEE and CEE, where handling greater than 15 days was achieved. The development of visible mold as a function of the different treatments are seen in Figure 4 a-b. All treatments had significant differences (P < .05). The coatings significantly decreased mold growth at the end of storage as shown in Figure 5.
In the NC treatment, mold growth was observed starting at day 3 on fruit inoculated with R. stolonifer and A. niger. As expected, the strawberries showed accelerated fungal growth between 3 and 5 days of storage. Additionally, the refrigeration system to which they were subjected did not  slow down the deterioration of the fruit ( Figure 5). Besides, the turgid, juicy, and soft texture of the strawberry fruit tissues generates ideal conditions for the growth and proliferation of various microorganisms (Lafarga et al., 2019). R. stolonifer is a microorganism that promotes wound diseases after harvest and is known as soft rot. A. niger is the most common species in the Aspergillus genus and causes black mold in fruit and vegetables (Jahani et al., 2020). Therefore, the inoculation of both fungi caused accelerated cell death in NC fruit.
The Gel/CMC coating reduced the rotting generated by the inoculation in strawberry fruit. However, the effect was not sufficient, and the rotting reached levels of almost 90% after 10 days of storage. The use of biopolymeric coatings with suitable physical, and microbiological properties has been recognized as an alternative for lengthening the shelf life of fresh vegetable products (Yousuf et al., 2018). Thus, the formation of a semi-permeable layer of Gel/CMC decelerated microbial growth in the fruit versus NC and extended the shelf life to 7 days. The use of Gel/CMC/AEE and Gel/CMC/ CEE significantly affected fungal development so that it was almost nil at day 5 after the inoculation of molds and varied between 30.15 and 32.39% after day 10; it was located in the range of 41.7 and 44.9% for both molds after day 15. At day 15, the treatment Gel/CMC/AEE caused inhibition but was not significantly higher than the Gel/CMC/CEE.
In addition to the beneficial effect that biopolymeric coatings can generate by themselves, they can also serve as a vehicle for other agents with some specific properties . Thus, the direct application of antimicrobial compounds on the surface of food can have a limited effect due to limited diffusion into the product; however, this transport is improved when a coating is used as a vehicle because an effective concentration is maintained on the surface to prevent or reduce microbial development (Yousuf et al., 2018).
The antifungal properties of AEE have been explained by the presence of persin (Z, Z-1-acetoxy-2-hydroxy-    4-oxoheneicosa-12, 15-diene) in addition to other substances such as epicatechins and other phenolics (Bowen et al., 2018). Likewise, it has been determined that the Hass avocado epicarp has a high content of condensable tannins (Jiménez-Velázquez et al., 2020) whose antifungal activity has been reported (Abu Zarin et al., 2016;Shohayeb et al., 2013). Similarly, coconut pericarp contains different types of polyphenols including catechins, epicatechins, kaempferol, condensable tannins (procyanidins B), ferulic acids, p-coumaric, etc. Its extract has been tested as an antimicrobial alternative against E. coli, S. enterica, S. aereus, F. pedrosoi, and R. stolonifer (Esquenazi et al., 2002;Sinsinwar et al., 2018). The fungicidal action has also been reported to be mediated by the interaction and composition of the different bioactive compounds and metabolites found in the extracts (Da Rocha Neto et al., 2019). The effect of natural extracts on the biopolymer matrix reduced the fungal growth caused by the inoculation of R. stolonifer and A. niger. Consequently, the shelf life of strawberries was extended to 15 days.

Conclusions
The results showed that the biopolymeric matrix of gelatin and carboxymethylcellulose applied on the surface of strawberries can extend their shelf life for 9 days at 2°C. On the other hand, extracts of avocado epicarp and coconut endocarp incorporated in the biopolymeric matrix extended strawberry fruit shelf life by more than 15 days. In addition, the shelf-life extension was accompanied by a significant delay in weight loss, softening, color behavior, and compositional changes (sugars and titratable acidity) compared with Gel/CMC coated fruit. Furthermore, the matrix incorporated with epicarp and endocarp extracts from avocado fruit (AEE) and coconut (CEE) inhibited the growth of R. stolonifer and A. niger in strawberries throughout storage.
Further research is needed to incorporate a more detailed mechanism through an appropriate experimental model for obtaining plant extracts and incorporating biopolymeric materials of a lipid nature to improve the hydrophiliclipophilic balance of the coating. Furthermore, a sensory analysis will be carried out with a panel of trained tasters in future experiments. In conclusion, this work gives a new strategy of avocado epicarp, and coconut endocarp utilization as antifungal extracts and their incorporation in a biopolymeric matrix that can extend fruit shelf-life.