Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds

ABSTRACT Amaranth has been of interest for its nutritional quality and its multiple benefits; however, there is little research on its toxicological effect. This work studied antinutritional compounds, the cytotoxic and genotoxic effect in the Amaranthus protein extract (PEAh). The content of lectins, tannins, saponins, and trypsin inhibitors was determined. Biological studies were conducted to determine toxicity in male CD-1 mice by intraperitoneal administration. The genotoxic potential was determined by the micronucleus test. The bone marrow cytotoxicity was determined by polychromatic erythrocyte (PE) rate and the cytotoxic effect was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Our results showed considerably high concentrations of lectins and trypsin inhibitors, lower concentrations of tannins and saponins in PEAh. The PEAh was toxic to mice, presenting genotoxic and cytotoxic damage to bone marrow and hepatocytes.


Introduction
Many people have argued that natural medicines are much safer than synthetic drugs (Valerio & Gonzales, 2005). This has caused exceptional growth in human exposure to natural products, as phytotherapeutic agents, phytopharmaceutical, and nutraceutical products. As a result, there has been a resurgence of scientific interest in the biological effects of natural products to guarantee the biological effect attributed to them and the possible toxicological risk to which consumers may be exposed. There is no regulatory system ensuring the safety and activity of natural products in most countries, and such products have not been sufficiently investigated analytically or toxicologically (Valerio & Gonzales, 2005). Currently, it has been shown that many plants used in traditional and folk medicines are potentially toxic, mutagenic, and carcinogenic (Déciga-Campos et al., 2007;Ferreira-Machado et al., 2004;Fonseca et al., 2000;Grobelnik Mlakar et al., 2009;Matthews et al., 2003;Teschke et al., 2009;Valerio & Gonzales, 2005).
One such plant of recently increased importance is the amaranth. It is an ancient plant belonging to the family of Amaranthaceae, which is believed to have originated from Central and South America (Gamel et al., 2006). Amaranthus hypochondriacus is a species considered native to Mexico (Sauer, 1950(Sauer, , 1967. This and other species of amaranth have been used in a wide variety of foods, such as cereals, candies, sauces, and porridges. Amaranth flours are used in the preparation of bread, tortillas, and desserts. The chemical and cosmetic industries have also taken advantage of the plant to make oils and dyes (Grobelnik Mlakar et al., 2009).
Amaranth cultivation dates back 5,000 to 7,000 years. The Mayans were probably the first to cultivate amaranth. In recent times, the amaranth crop reappeared, not only in Mexico and Central America but also in the Andean territories of South America, as well as in some Asian, European, and African countries (Escudero et al., 2004). The species of this plant are receiving significant attention in developing countries to fight against protein malnutrition because it has proteins of good quality, a favorable composition of amino acids and fiber, and high digestibility coefficients of nutrients. Also, the seeds of amaranth can undergo different treatments to increase their nutritional proprieties (Escudero et al., 2004;Písaříková et al., 2011).
Different studies have characterized the seeds of amaranth, showing its antioxidant activity (Lehmann et al., 1994), hypocholesterolemic properties (Klimczak et al., 2002), and its potent anticarcinogenic effects (Barrio & Añón, 2010). Unfortunately, controversial data exist about the balance in its beneficial and/or toxic potential. Some evidence shows that amaranth also contains compounds that reduce its nutritional quality by reducing the absorption and/or nutrients utilization (Correa et al., 1986), adversely affecting health. These include lectins (Rinderle et al., 1990), saponins, tannins, and trypsin inhibitors (Ivleva et al., 2000). They have been related to various alterations in the gastrointestinal tract, producing a poor absorption of nutrients and, in the long term, possible malnutrition. Some studies have shown that lectins can produce toxic effects in various biological models, both in vivo and in vitro (Gonzalez de Mejia et al., 2005). In the same way, it has been observed that trypsin inhibitors decrease the digestibility of animal proteins (Palliyeguru et al., 2011). Saponins and tannins have been shown to diminish the growth of laboratory animals due to their ability to trap metal ions relevant to the enzymatic activity of the digestion of various nutrients (Griffiths & Moseley, 1980;Laurena et al., 1984;Valerio & Gonzales, 2005). All these factors contribute to the analysis of the plant to find its beneficial and harmful properties; therefore, the proposed aim was to evaluate the content of antinutritional compounds, the cytotoxic and genotoxic effect of the proteins extracted from the seeds of Amaranthus hypochondriacus.

Animals
Thirty-five male mice strain CD-1 with a mean weight of 13 g were used in the micronucleus assay. They were obtained from the Institute of Health Sciences-UAEH (Pachuca, Hidalgo, Mexico), and maintained under standard conditions in metallic cages at a temperature of 23 ± 2°C and 50 ± 10% humidity, with feed (TEKLAD Global 2018S, Harlan, Mexico City) and water ad libitum, in a 12 h light-dark period. The adjustment time period for the animals was one week before the treatments. Male Fisher rats weighing 180-200 g were employed to obtain hepatocytes. The animals were obtained from the Production Unit of Experimental Laboratory Animals (UPEAL-Cinvestav, Mexico City, Mexico). These were fed ad libitum rodent chow and tap water, and before liver perfusion, were fasted for 18 h.

Human erythrocytes
Peripheral blood erythrocytes Type O were obtained from one voluntary healthy male donor. All the procedures were realized by the Official Mexican Standard NOM-007-SSA3-2011 and NOM-253-SSA1-2012. The subject provided written informed consent, and the protocol was approved by the Ethics and Research Committee of the Autonomous University of Hidalgo State with the number CEEI-000011-2019.

Plants seeds
Amaranthus hypochondriacus seeds (amaranth) used in the experiment were obtained directly from farmers in the town of Progreso Hidalgo, Mexico 20°14′53″N 99°11′23″O. The seeds were cleaned. The less damaged ones were selected, and these were stored at 5°C until use.

Protein extraction of Amaranthus hypochondriacus seeds
Proteins were obtained from the Amaranthus hypochondriacus seeds flour by shaking for 16 h at 4°C in 10 mM phosphate-buffered saline (PBS) solution. The mixture was centrifuged at 12,000 g for 60 min to yield the protein extract of A. hypochondriacus (PEAh). The PEAh was dialyzed overnight against deionized water at 4°C, then lyophilized and stored at −20°C. The protein concentration of Amaranthus hypochondriacus extract was determined by the Lowry method of using bovine serum albumin as standard (Waterborg & Matthews, 1984).

Lectin activity
The lectin was identified by hemagglutination, and the tests were carried out in microtiter 96-well (U-shaped) plates. The lectin was diluted serially (2-fold), adjusting the sample volume in each well to 50 µL with PBS. Each diluted sample was added to 50 µL of the 2% suspension of trypsinized human erythrocytes type O; these were obtained by incubation globular packages with trypsin (0.6 mg/mL), in PBS and incubated for one hour at 37°C in constant shaking. The trypsinized human erythrocytes exposed to protein sample diluted were incubated for 1 h at room temperature and then observed for positive agglutination. The titer was defined as the reciprocal of the highest dilution showing detectable agglutination (Jaffé et al., 1972).

Determination of tannins
Tannins were determined according to the modified vanillin-HCl method of Price et al. (1978) using (+)-catechin (Sigma Chemical Co, St Louis, MO, USA) as standard. Tannin content was expressed as mg of catechin (CE) per gram of sample (mg CE/g) (Price et al., 1978).

Determination of Trypsin Inhibitors (TI)
Trypsin inhibitory activity was measured as the residual trypsin activity, using BAPNA (N-benzoyl-DL-arginine p-nitroanilide) as substrate (Martinez & Moyano, 2003). Trypsin inhibitory unit (TIU) was defined as the amount of inhibitor that inhibits 1 μg of pure trypsin. Total trypsin inhibitory activity was expressed as TIU per mg of lyophilized sample (TIU/mg). The reported results are the means from three separate measurements.

Determination of saponin
Saponins were extracted by the Soxhlet method using methanol-water mixture (85:15 v/v) for 2 h, and rotary evaporated to dryness and resuspended in saline solution (0.9%). The tests were carried out by the serial dilution method proposed by Girón (1992), using type O human erythrocytes. Results were expressed as hemolytic units per milligram. The reported results are the means from three separate measurements.

Evaluation of the toxic effect of PEAh
Hepatocytes were isolated from male fisher rats (180-200 g) by the collagenase perfusion method (Mendoza-Figueroa et al., 1979). The cell was collected in a petri dish in Dulbecco's modified Eagle's medium (DMEM) F12 with 7% bovine serum, 5 μg insulin/mL, 100 U penicillin G/mL, and 100 μg streptomycin/mL. The cultures were maintained at 37°C in 5% CO 2 in air (Sanyo CO 2 Incubator US-Canada, Sanyo Scientific).

Cell viability assay
The hepatocytes were cultured for 24 h in DMEM medium supplemented with fetal bovine serum and antibiotics in a 4% CO 2 and humid atmosphere at 37°C. The cell viability was measured by the tetrazolium dye colorimetric test, using (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) (Hansen et al., 1989). The hepatocytes were cultured for 24 h in DMEM medium supplemented with fetal bovine serum and antibiotics in a 4% CO2 and humid atmosphere at 37°C. The cell viability was measured by the tetrazolium dye colorimetric test, using MTT (Hansen et al., 1989). Hepatocytes were treated with various concentrations of protein extract (0 to 5,550 μg/mL) under the same conditions previously described for 24 h. Then, MTT was added to the medium until getting a 5 mg/mL concentration and incubated for 3 h. At the end of that, the medium was removed, and 100 μL of dimethyl sulfoxide was added to each well to dissolve the formazan produced. The absorbance of each well was measured at 540 nm in a microplate reader (EPOCH, Biotek, Biotek Instruments Inc USA). The cell viability was expressed as a percentage of the value of the control culture.

Lethal median dose (LD 50 )
Lethal median dose was determinated by the Lorke method (1983). Male mice CD-1 were randomly divided into seven lots and PEAh was administrated intraperitoneal via. The assay was made in two steps: in the first step, doses of 10, 100, and 1000 mg/kg of PEAh were administered intraperitoneally (i.p) and showed no lethality among the animals; then the effect of 1600, 2900, and 5000 mg/kg was tested, and mortality appeared among the treated mice.

Evaluation of the genotoxic capacity of PEAh by the micronucleus assay
After calculating DL 50 , we considered using doses of 50, 150, 250 mg/kg of PEAh to determine the genotoxic potential. The Committee of Ethics and biosecurity of the Institute of Sciences of the Health approved the protocol of micronucleus assay. Animals were organized into groups of five individuals each: a negative control group (saline solution 0.9%), a group positive treated with daunorubicin (DAU 3 mg/kg), and three groups treated with PEAh (50, 150, and 250 mg/kg) respectively. The PEAh were administered daily for a week, intraperitoneally, and according to the experimental groups mentioned. The DAU was injected in a single dose intraperitoneally. Rate of micronucleated polychromatic erythrocytes (MNPE) was determined before the experimental treatment and at 24, 48, 72, and 96 h after treatment. Two blood smears were made from the tail of each animal, fixed in methanol for 5 min, and stained for 20 min with a 4% Giemsa solution prepared in phosphatebuffered saline, at a pH of 6.8 (Madrigal-Santillán et al., 2007). One thousand erythrocytes per animal were scored to determine the rate of MNPE. To evaluate bone marrow cytotoxicity, we scored 1000 erythrocytes per animal and established the rate of polychromatic erythrocytes (PE) concerning the number of normochromic erythrocytes (PE/NE index).

Data analysis
The data obtained were analyzed with the statistical program GRAPHPAD INSTAT (San Diego, CA, USA) version 3.0, using ANOVA to determine if data were normally distributed within all groups. After that, a Tukey test (p < .05) was performed to establish the statistical differences between groups in each assay.

Antinutritional compounds in A. hypochondriacus protein extract
According to the extraction method employed, the protein concentration in the extract was 2.1428 mg/mL, founding antinutritional compounds, including those of protein origin as lectin and trypsin inhibitors, as well as tannins and such as presented in Table 1. Figure 1 shows the cytotoxic effect of PEAh on primary cultured rat hepatocytes after 24 h of exposure. PEAh presented a dose depending cytotoxic effect. As can be seen, the hepatocyte cell viability decreases by 47.44% at the protein concentration of 1 μg/mL, and at 10 μg/mL the viability reaches 50%, obtaining the ID 50 . In contrast, at the maximum concentration, the viability decreased to 18%. Acute toxicity assay of Amaranth proteins on mice presented a lethal dose (LD 50 ) of 1254.9 mg/Kg of bodyweight, which classified the extract as moderately toxic. These results made it possible to establish the experimental conditions for the study of genotoxicity and cytotoxicity in mice not to cause death during the tests.

Hepatotoxicity of the antinutritional compounds of A. hypochondriacus protein extract
The results on the frequency of MNPE in the studied groups are shown in Figure 2, observing that the animals belonging to the control group had no micronucleus increase in the four days of the experiment, having an average value of 1.0 MN/1000 PE. The mice treated with DAU (3 mg/kg) manifested a significant increase (p < .05) after 24 h of treatment with the most severe genotoxic damage. At 48 h, the micronucleus reached more than 60 MN/1000 PE. For the animals administered with the PEAh, there was no significant statistical difference (p < .05) Figure 1. Cytotoxic effect of Amaranth protein extract on primary rat hepatocytes culture.

Figura 1.
Efecto citotóxico del extracto de proteína de amaranto en cultivo primario de hepatocitos de rata Note: Results are presented as the percentage of viable cells (untreated cells were considered as 100% of viability). Each value represents the mean ± standard deviation obtained from triplicate plates. Nota: Los resultados se presentan como el porcentaje de células viables (las células no tratadas se consideraron con el 100% de viabilidad). Cada valor representa la media ± desviación estándar obtenida de placas por triplicado.  Note: Values represent the mean ± standard deviation of five mice per group. The superscript letters show statistically significant differences as follows: a-concerning the control value, b-concerning the value in the group treated with DAU; and c-concerning the initial time in the same group. ANOVA and Tukey tests (p < .05). Nota: Los valores representan la media ± desviación estándar de cinco ratones por grupo. Las letras en superíndice muestran diferencias estadísticamente significativas como sigue: acon respecto al valor de control, b-con respecto al valor en el grupo tratado con DAU; y c-sobre el tiempo inicial en el mismo grupo. Pruebas ANOVA y Tukey (p <,05). regarding the control, at concentrations of 50 and 150 mg/kg during the test. It was observed an increase of MNPE rate at the doses of 150 and 250 mg/Kg. The greatest damage to genetic material was at the dose of 250 mg/kg during the 72 and 96 hours of treatment, obtaining a mean value of 24 MN/1000 PE; suggesting that the PEAh is a dose-dependent genotoxic agent.

Bone marrow cytotoxicity of the Antinutritional compounds of A. hypochondriacus protein extract
Results of the relation between PE and the number of normochromatic erythrocytes (PE/NE index) are shown in Table  2. This is an indicator of bone marrow cytotoxicity. At the beginning of the experiment, the PE/NE index was similar in all the groups. In the successive hours of treatment, the control group showed no significant variation in the index. The mice administered with DAU showed a significant increase in the rate of PE/NE at 96 h of treatment, equivalent to 72% concerning the control group level. In relation to the animals treated with the PEAhs, the PE/NE index increased with time. However, the concentration of 250 mg/kg reached a maximum at 48 h, followed by a fast decrease, which one statistically significant after 24 h of treatment in all concentrations analyzed. Based on these results, it can be inferred that the protein extract obtained from amaranth is cytotoxic to the bone marrow because of the increase in erythropoiesis.

Discussion
Amaranth grain has the potential to contribute to improving the nutrition of populations, especially in developing countries, because it has nutritional and functional properties (Muyonga et al., 2008). The presence of some compounds has been reported, such as phenols, fatty acids, tocopherols, carotenoids and others that contribute to health, reducing the risk of oxidative stress related to chronic diseases (Jo et al., 2015;López et al., 2013;Tang & Tsao, 2017). However, several researchers have demonstrated that amaranth seeds may contain antinutritional compounds, which could affect their nutritional value and limit their use. It has been observed that the content of compounds, such as trypsin inhibitors, phytates, saponins, tannins, and lectins, is related to the genus (Flavin & Pennington, 1982).
In this study, we analyzed the antinutritional compounds in the protein extract and determined the genotoxic and cytotoxic effects of PEAh, because this is the principal species cultivated and consumed in Mexico. Likewise, we chose to study the protein extract because of the significant interest for this grain due to its good quality proteins and being gluten-free (Alvarez-Jubete et al., 2010).
Contrary to its high nutritional quality, amaranth seeds contain antinutritional compounds, such as tannins, lectins, saponins, and trypsin inhibitors, which affect this plant's nutritional value and consumers' health (Flavin & Pennington, 1982). The concentration of antinutritional compounds depends on the species of Amaranthus, the methods employed for extraction and quantification, and the differences in time and place where amaranth was cultivated (Correa et al., 1986). This may be because the authors employed different extraction and quantification methods, and on the other hand, there are differences in terms of place and time of seed cultivation.
In addition to the function of storage proteins in seed, the globulins have a secondary activity such as protease inhibitory activity (Rassam & Laing, 2006) and lectin-like activity (Soares et al., 2007). Sathe and Deshpande (2003) reported that lectins are found in albumin and globulin fractions and that globulin in cereals can hemagglutinate rabbit red blood cells, and this lectin activity resides in the a-subunit of the globulin (Langston-Unkefer & Gade, 1984).
As in other research, the present work demonstrates that the hemagglutinating activity of lectins in PAEh has high activity. The lectins in amaranth seeds have been previously reported in A. hypochondryacus, A. leucocarpus, and A. caudatus, which are recognized as homodimeric proteins (Rinderle et al., 1989;Ozeki et al., 1996;Zenteno et al., 1992;Zenteno & Ochoa, 1988).
The lectins are widely distributed in plant tissues and are particularly abundant in seeds, reaching up to 10% of total proteins. However, they have also been located in smaller quantities in leaf stems, roots, and barks (Sharon & Lis, 2004). These proteins have been reported to be cytotoxic in several cell lines such as SW480, C33-A, Hela, 3T3 (Barzideh et al., 2014;, which was observed in the present work since the protein extract was rich in lectins. This result allows attributing the Table 2. Relationship between the numbers of polychromatic erythrocytes and the number of normochromatic erythrocytes (PE/NE index).

Hours
Control ( Values represent the mean ± standard deviation of five mice per group. The letters show significant statistical differences as follows: a With respect to the control value; b With respect to the value in the group treated with daunorubicin (DAU); and c With respect to the initial time in the same group. ANOVA and Tukey tests (p < .05). Los valores representan la media ± desviación estándar de cinco ratones por grupo. Las letras muestran diferencias estadísticas significativas como sigue: a con respecto al valor de control; b con respecto al valor en el grupo tratado con daunorrubicina (DAU); y c con respecto al tiempo inicial en el mismo grupo. Pruebas ANOVA y Tukey (p < ,05).
observed cytotoxic effect to this type of protein. Since lectins can bind to cell membrane glycoconjugates in the digestive tract, they affect the transport of nutrients; this is why there has been increased interest in their antitumor effect in recent years, which prevents cell interactions causing cell damage (Quiroga et al., 2015).
Lectins, even in very low concentrations, can cause death to different laboratory animals (Reynoso-Camacho et al., 2003;Benson et al., 2011). The antinutritional compounds reported in this work, especially lectins, were found in high concentrations and may be responsible for the death of mice. It is well known that this type of protein can cause the death of laboratory animals by damaging several organs, promoting malabsorption of nutrients, inhibiting protein synthesis, and increasing the development of bacteria, among others (Endo et al., 1991;Pusztai & Bardocz, 1996).
Regarding the trypsin inhibitor level found in the PEAh, this was superior to in A. cruentus protein extract (Escudero et al., 2004). At the same time, the trypsin level was lower than reported in amaranth flours. Trypsin inhibitors in amaranth seeds have been reported to range from 938 to 2100 TIU/g for the black seeds, and from 300 to 5150 TIU/g units for the yellow seeds. These differences may be due to the species and the analytical methods employed (Correa et al., 1986;Valdes-Rodriguez et al., 1993). It has been demonstrated that this type of protease inhibitors present cytotoxic effects in some malignant and normal cell lines, such as MCF-7, HeLa, A549, HEK (Lanza et al., 2004;Patriota et al., 2020;Shamsi et al., 2017) and can also cause hypertrophy and hyperplasia of the pancreas, and lead to adenomas and carcinomas of the exocrine pancreas in rats (Hathcock, 1991). This supports the results obtained, since trypsin inhibitors were found in the amaranth extract, which may be contributing to the cytotoxic and toxicological effects in animals.
The concentration of tannins reported in the present work is in accordance with Akin-Idowu et al. (2017) and Venskutonis and Kraujalis (2013) results, who studied different species of Amaranthus, but are lower than that reported for amaranth grains. These differences relate to the fact that the compounds were determined in a protein extract in the present work, and in the other studies, it was from the seeds. However, it is known that tannins are preferably found in the seed coat than in perisperm, and their concentration depends on the color of the seed, being higher in colorful and dark seeds (Akin-Idowu et al., 2017;Osuntogun & Oke, 1983).
The hemolytic activity of saponins in PEAh was previously analyzed. The activity of this compound was lower in PEAh than in other amaranth species, such as A. hypochondriacus, A.cruentus, and A. hybridus, which differ considerably in their biological activity (Kohda et al., 1991;Oleszek et al., 1999).
The genotoxic assay showed that at a low concentration of PEAh, the quantity of MNPE did not show a significant statistical difference (p < .05) for the negative control. This result suggested that at these concentrations, the PEAh does not present a genotoxic effect. However, at the highest concentration, the proteins extract exerted genotoxic activity in the in vivo test system, inducing the production of MNPE. The cytotoxic effect observed in the in vitro and in vivo assays showed that the protein extract exerted a dose-dependent effect, causing damage to both the hepatocytes and the bone marrow.
The genotoxic damage found for PEAh is supported by the findings of other studies, since it has been reported that protein extract and pure lectins in plants present genotoxic effect, demonstrated by micronucleus, ames, and comet assays (Faheina-Martins et al., 2011;Patriota et al., 2020;Rolim et al., 2011).
The findings of this work suggest that the protein extract contains compounds responsible for the observed biological damage. Compounds such as lectins and trypsin inhibitors are globular proteins with anti-physiological effects and were extracted with the method used to obtain the PEAh. The concentration at which some of them were found may have been high enough to produce the observed cytotoxic and genotoxic damage. Other types of anti-physiological compounds, such as tannins and saponins, were found. Although their concentration were low in comparison to other reports, they could also collaborate in the biological damage observed. (Akubugwo et al., 2007;Venskutonis & Kraujalis, 2013) Several papers have been published showing the cytotoxic effect of lectins and extracts rich in lectins on normal and malignant cells (Reynoso-Camacho et al., 2003;Gonzalez de Mejia et al., 2005;. Likewise, trypsin inhibitors, tannins, and saponins, from different plant sources, have also been shown to have cytotoxic activity in several cell lines (Akhtar et al., 2017;Buyukleyla et al., 2012;Jaramillo-Carmona, Guillén-Bejarano et al., 2018;Jaramillo-García, Trindade et al., 2018;Mbaveng et al., 2018;Shamsi et al., 2017;Zhu et al., 2018). Saponins, trypsin inhibitors, and tannins from different sources have been reported as genotoxic, inducing the formation of micronuclei and chromosomal aberrations (Akhtar et al., 2017;Blaszkowska et al., 2009;Buyukleyla et al., 2012;Dauer et al., 2003;Demma et al., 2013).
It can be inferred that the antinutritional compounds found are related to the toxicological effect since, as mentioned above, lectins, trypsin inhibitors, tannins and saponins have shown cytotoxic and genotoxic effects by themselves. Since all these compounds were found in PEAh, it can be deduced that all of them contribute to the cytotoxic and genotoxic effects observed. Although lectins and trypsin inhibitors may be the principal responsible for the cyto-and genotoxic effect found, they were found in higher concentrations; however, tannins and saponins could also contribute to the adverse effect.

Conclusions
Our results showed considerably high concentrations of lectins and trypsin inhibitors, lower concentrations of tannins and saponins in PEAh. The PEAh was toxic to mice, presenting genotoxic and cytotoxic damage to bone marrow and hepatocytes. In this study, high concentrations of lectins and low concentrations of tannins, saponins and trypsin inhibitors were found in PEAH, which have been reported to have toxicological effects in cell lines and in laboratory animals, even causing death.
PEAh showed a dose-dependent cytotoxic effect on hepatocytes, determining the ID 50 at 10 μg/mL, as well, a time-and dose-dependent genotoxic effect of PEAh.
The Bone marrow cytotoxicity of PEAhs, corresponded whit the PE/NE index, increased with time. The major effect was observed at 96 h at the concentrations of 50 and 150 mg/kg, while at 250 mg/kg the greatest effect was found at 48 h.
The effects observed in cytotoxicity and genotoxicity studies, caused by PEAh, can be attributed to the presence of the antinutritional compounds found in the protein extract.
Future research is required to explain the mechanisms of action of these proteins; in addition to, purify the main antinutritional compounds found in the extract as well as evaluate toxicological effects in vitro studies with other cell lines.