Evaluation of the physiological alterations in Ceratophyllum demersum L along a diurnally changing solar irradiance gradient

ABSTRACT This study was implemented to investigate the diurnal biochemical changes that occur in Ceratophyllum demersum in four growth habitats under in situ experiments. The results showed that compared to the other treatments, the open-flow treatments had the highest hydrogen peroxide (H2O2) concentration that occurred with the acceleration of antioxidant activity at midday, mainly due to the independent effect of irradiance and flow movement. The plant diurnal H2O2 and antioxidant activity rhythms had varying fluctuations, and peroxidase (POD) activity showed a rapid response to oxidative stress. The increase in POD activity followed a more similar pattern aligned with H2O2 synthesis, while catalase (CAT) and ascorbate peroxidase (APX) activities were dominant in the late hours of the day. The ability of this species to successfully grow in these habitats is related to the adaption of the diurnal cycles of antioxidant activity according to flow and irradiance changes.


Introduction
The growth and development of aquatic macrophytes depend on various environmental factors. Among these factors, water, temperature, flow and sediment nutrients play important roles (Dale 1986;Dawson and Szoszkiewicz 1999). Therefore, aquatic habitats are heterogeneous environments and are characterized by temporal and spatial fluctuations in chemical and physical parameters . In terms of zonation, aquatic plants can grow in stagnant, flowing or shaded habitats, which are classified into four categories: floating, floating leaves, submerged or emergent (Chambers and Kalff 1987).
Over the past few decades, aquatic habitats have been affected by anthropogenic activities, causing drastic changes in macrophyte species diversity (Giller 2005;Zhang et al. 2015). For example, the construction of dams and reservoirs changes flow dynamics and influences the abundance of macrophytes. Water level and flow dynamics influence macrophytes in different ways (Mjelde et al. 2013). Hydraulic forces generated by waves result in physical changes in macrophytes, e.g. growth retardation (Madsen et al. 2001;Ellawala et al. 2011), stem breakage and uprooting (Riis and Biggs 2003). Some submerged aquatic macrophytes, for instance, increase leaf nutrient uptake and oxygen absorption with moderate water flows (Stevens and Hurd 1997) by thinning the leaf blade .
The same environmental factors exceed their threshold levels, and they become stressors for aquatic plant communities. Compared to other abiotic stressors, solar irradiance fluctuations are common in aquatic habitats and have been reviewed in different studies (López-Figueroa 1992;Conde-Álvarez et al. 2011;Yuan et al. 2016). In addition, photosynthesis alterations in aquatic plants due to physical restrictions and light availability are much more significant than those in terrestrial plants (Sand-Jensen 1997). In aquatic ecosystems, light energy is one of the key determinants that is crucial for photosynthesis because light provides energy for electron transport (Binzer et al. 2006), and thus, its limitation retards the growth of macrophytes ). On the other hand, some aquatic plant species prefer shaded natural environments and behave as shade-adapted species (Barko and Smart 1986). These irradiance and flow changes may have positive or negative consequences for macrophytes, which may be related to the type of macrophyte species. Thus, based on these resource fluctuations, adaptive strategies may be developed for survival (Chen et al. 2016). Biochemical changes that take place at the cellular level might be an effective adaptive mechanism for abiotic stress that is controlled genetically and physiologically  and may be expressed as an antioxidant system. The activation of an antioxidant system occurs due to different abiotic stressors. Changing environmental factors disrupt plant cell homeostasis and cause an imbalance in internal plant cell metabolism (Aro et al. 1993), eventually causing oxidative stress. If received light energy exceeds the absorbance capacity of chlorophyll, then the generation of reactive oxygen species (ROS) is dramatically elevated (Nishiyama et al. 2001), which reduces photosynthesis potential (Powles 1984;Nishiyama et al. 2004). Often, this imbalance leads to the generation of ROS. Major ROS are identified as singlet oxygen, superoxide and hydrogen peroxide (H 2 O 2 ) that accumulate within plant cells (Polesskaya et al. 2006). Water cycle-produced ROS are rapidly scavenged by antioxidative enzymes (Asada 2006).
Ceratophyllum demersum (Ceratophyllaceae) is a perennial submerged macrophyte that grows in streams, lakes, and shallow aquatic habitats. C. demersum originated in North America and is presently considered an invasive species in many areas of the world (DiTomaso et al. 2013). The invasion success of this noxious species depends on its rapid spreading and adaptation to a wide range of aquatic habitats (Mishra et al. 2009). Moreover, based on its growing area, this species expresses changing phenotypic plasticity. For example, the development of modified leaves that attach to deep water sediment (Sculthorpe 1967;Keskinkan et al. 2004) is a good adaptation for survival in deep water habitats. Most studies related to C. demersum have focused on sediment effects (Stiers et al. 2011), biotransformation (Stiers et al. 2011), and phytoremediation (Aravind and Prasad 2004;Mishra et al. 2008). However, little attention has been given to understanding the diurnal biochemical adaptation changes that occur in different C. demersum habitats.
In the present study, we experimentally simulated four potential habitats considering flow regime and solar irradiance, and we hypothesized that biochemical changes may vary diurnally with each simulated condition. Thus, our objectives were (1) to evaluate adaptation to light irradiance and water flow based on photosynthesis (electron transport rates (ETRs) and dark yield) and (2) to assess diurnal biochemical changes in C. demersum through enzyme activities (ascorbic peroxidase (APX), guaiacol peroxidase (POD), and catalase (CAT)) and ROS that corresponded to the flow and irradiance. The results provide an understanding of environmental conditions suitable for this plant, and this understanding may help effectively minimize the invasion potential of this noxious weed through alteration of the optimum growing conditions.

Plant materials
Experimental plants were collected from the Toda River (35°4 8'13.9"N, 139°39'23.8"E), Japan, where this plant is considered a weed. After collection, plants were immediately transported to the indoor experimental facility located in the Department of Environmental Science, Saitama University, Japan (35°53'51.4"N, 139°60'77.8"E). Prior to the experiment, approximately 8 cm of C. demersum apical shoots were cultivated separately within three glass microcosms (60 cm × 30 cm × 36 cm) in the indoor laboratory for up to 4 weeks. Each microcosm was aerated to provide sufficient dissolved oxygen, and the growth chamber temperature was maintained at 23 ± 2°C with a light intensity of approximately 100 µmol m −2 s −1 and a 16:8 light dark cycle. The nutrient medium was a 5% Hoagland solution to meet the nutrient requirements of the plant (Hoagland and Arnon 1950).
After one month, homogeneously developed plants were randomly selected, and propagules that were 10 cm long were cultivated within the PVC pots (6 cm in diameter and 7 cm high) for further acclimatization in an outdoor greenhouse. Plant fragment sizes were determined based on previous literature (Ellawala et al. 2011;Zaman and Asaeda 2013). The room temperature was maintained at 25 ± 2°C under a natural light: dark cycle (16:8) for 1 week before starting the experiment. A total of 300 mL of thoroughly washed commercial river sand (90% <1 mm particle size) was used for the planting medium, and the plant density was 6 fragments per pot.

Experimental setup
All experiments were conducted in an outdoor flume channel (240 cm × 25 cm × 22 cm) at Saitama University, Japan, in May 2018. After pretreatment for 7 days, each set of 15 PVC pots (6 cm in diameter and 7 cm high) was transferred into three glass microcosms (40 cm ×25 cm ×28 cm) that represented one treatment. To minimize adaptation shock to the plants, tanks were filled with tap water and aerated for 24 h. We incorporated four conditions, which were static open, static shaded, open flow, and shaded flow treatments, consisting of 3 replicates (Figure 1). Black-colored commercially purchased polyethylene shade sheets (DIY, Doito, Japan) were cut into equal sizes and used for shade. The solar irradiance reduction efficiency of a particular shade cloth evaluated using a light intensity meter (Apogee, MQ-200, USA) was 72%. The outer surface of the glass tank was covered by 70% shade sheets, whereas uncovered tanks served as the static open environment and received 100% ambient photosynthetic active radiation (PAR). In contrast to the static treatments, the flume experiment setup was maintained, and water was circulated in the flume channel through regulated water pumps. Half of the flume channel was covered by a shade sheet and represented the shaded flow treatment. Just above the plant canopy, the water velocity was measured with an ultrasonic velocimeter (Tokyo Keisoku Co. Ltd, Japan). The mean flow velocity of water was approximately 25 cms −1 , which was comparable to the mean flow velocities found in Japanese field conditions .
Evaporation losses were compensated for by using preaerated tap water to maintain a constant water level (18 cm) among the four treatments. The water pH and temperature were measured using a portable water quality meter (Model No. U-53, Horiba, Japan). The observed water temperature remained constant over the experimental period at approximately 23.5°C ± 2°C, and the pH was within the range of 7.6 ± 0.3. The PAR in each treatment was recorded in three-hour intervals using a digital light intensity meter (Apogee, MQ-200, USA). After adjusting the treatment conditions, the experiment was continued for 5 days. On the last day of the experiment, three planting pots from each treatment were randomly selected for the biochemical assays every 3 h. Samples were collected between 6:00 am to 6:00 pm during the daylight regime for biochemical analysis.

Photosynthesis measurements
Photosynthesis was measured with a pulse amplitude modulation (PAM) fluorometer (Junior-PAM, Walz, Germany) at 12:00 pm on the day of harvest. Apical parts of the C. demersum shoots were connected to the junior PAM. Plants were adapted to the dark for 15 min before light curve measurements of 25,45,65,90,125,190,285,420,625,820, and 1150 µmol m −2 s −1 were obtained. Each irradiation level was applied with a saturation flash (pulse length of 2.5 s), which was set by the PAM, for 1 min. Quantum yield was determined by the software WinControl-3. Thereafter, the relative ETRs of photosystem II were calculated using the model of (Walsby 1997). There were 4 replicates for each treatment.

Photosynthetic pigment analysis
The chlorophyll a, chlorophyll b and carotenoid contents were measured spectrophotometrically after extractions in N,Ndimethylformamide for 24 h. Green-colored supernatants were obtained, and the absorbance was read at wavelengths of 664, 647 and 480 nm using a UV spectrophotometer (Wellburn 1994). The pigment content was calculated using the following formulas: Chlorophyll a (mg/mL) = 11.65A 664 − 2.69A 647 (1)

Antioxidant enzyme analysis
Harvested C. demersum plants were thoroughly cleaned and blotted, and approximately 100 mg of fresh plant samples were extracted in chilled (50 mM, pH 6.0) potassium phosphate buffer with 2% (w/v) polyvinylpyrrolidone (PVP). Homogenate was centrifuged at 3000 g for 10 min at 4°C. The resultant supernatant was immediately stored at −80°C for further biochemical analysis.
H 2 O 2 concentrations were estimated using a standard curve prepared from a known concentrations of H 2 O 2 following the method of (Jana and Choudhuri 1982). The reaction mixture contained 750 µL of enzyme extract and 2.5 mL of 1% (v/v) TiSO 4 in 20% (v/v) H 2 SO 4 . The intensity of color development was measured spectrophotometrically at 410 nm.
The APX activity (EC 1.11.1.11) was measured in a reaction mixture that contained 100 µL of enzyme extract, 200 µL of 0.5 mM ascorbic acid and 2.0 mL of 50 mM potassium phosphate buffer (pH 7.0). The reaction was started by the addition of 60 µL of 1 mM H 2 O 2 . The absorbance change was recorded at 290 nm every 10 s for a total of 3 min (Nakano and Asada 1981). The results are presented in µmol min− 1 g −1 FW.
The CAT activity (EC 1.11.1.6) was determined following the method of (Aebi 1984). The reaction mixture contained 100 µL of 10 mM H 2 O 2 , 2.00 mL of 100 mM potassium phosphate buffer (pH 7.0), and 500 µL of enzyme extract. The decrease in absorbance at 240 nm was recorded for 10 s for 3 min. The CAT activity was evaluated using the extinction coefficient of 40 mM −1 cm −1 .
For the measurement of the POD activity (EC 1.11.1.7), the reaction mixture contained 3.0 mL that had a pH 6, 50 mM potassium phosphate buffer, 50 µL of 0.2 mol guaiacol, 40 µL of 30 mM H 2 O 2 and 100 µL enzyme (MacAdam et al. 1992). The POD activity was obtained using an extinction coefficient of 26.6 mM −1 cm −1 . Each enzyme activity above is represented as µmol min− 1 g− 1 FW. The experiments were replicated three times (n = 3) for each growth condition.

Statistical analyses
Two-way analysis of variance (ANOVA) was performed for all corresponding variables when normality and homogeneity assumptions were met. In this study, we considered flow conditions (flow vs static) and irradiance level (open vs shaded) as fixed factors. When a significant effect was found, one-way ANOVA was carried out to test differences between treatments in each time interval (Gómez et al. 1998). The data were expressed before by their means and standard deviations followed by Tukey's post hoc test (p < 0.05) to identify homogeneous subgroups of significantly different means. All statistical analyses were performed using SPSS version 16.0 (SPSS, Chicago, IL, USA).

Photosynthesis responses
Dark yield responded significantly to the interactive effects of water flow and irradiance at midday (p < 0.05) ( Table 1). The measured rETR in response to increasing irradiance up to 1200 µmol m −2 s −1 showed notable variation among the four treatments (Figure 2). In comparison to the other plants, the plants subjected to the open flow treatment had the highest responsiveness to light in this experiment, while static shading resulted in the lowest responsiveness. The light curves of shaded plants in both the static and flow treatments exhibited a lower response for the given irradiance sequences compared to the plants in the unshaded treatments (Figure 2). Based on the two-way ANOVA, at noon, the rETR max was influenced by irradiance, and there was no significant interaction between flow dynamics and irradiance (p < 0.05) ( Table 1).

Pigment concentration
At 06:00 am, the Chl a concentration was significantly influenced by the interaction of water flow and irradiance level (p < 0.05) ( Figure 3A). Of the plants in the different treatments, the plants subjected to static open water had the highest Chl a concentration over the experimental period, whereas the plants subjected to shade exhibited the lowest pigment content ( Figure 3A). Between 06:00 am and 12:00 pm, plants subjected to open flow and shaded flow conditions had Chl a concentrations that followed a relatively linear trend that reached a peak at 12:00 pm. However, the opposite scenario was observed for Chl b during the middle of the day, as it was influenced only by the irradiance level (p < 0.05), but there were no interactive effects of flow and irradiance (Table 1). During the diurnal cycle at 06:00 pm, all treatments showed Chl a concentrations of approximately 500 µg g −1 FW except for that of the static open treatment. In the static open treatment, Chl b followed similar diurnal fluctuations to those of Chl a and continued to increase to approximately 350 µg g −1 FW between 03:00 pm and 06:00 pm ( Figure 3B). Compared to the steadier trend of the static open treatment, the other three treatments followed a more symmetrical pattern in which the concentration peaked at noon. The highest carotenoid levels were observed in the static open flow treatment, while the lowest carotenoid levels were found in the shaded flow treatment ( Figure 3C). At 06:00 am and 06:00 pm, water flow and irradiance interactively affected the carotenoid concentration, whereas the irradiance factor had a significant effect only at 12:00 pm (F = 7.973, p < 0.05) ( Table 1). The carotenoids peaked at mid-day at 44.35% compared to those in the early morning in the open flow treatment ( Figure 3C). Thereafter, the concentration (179 µg g −1 FW) remained almost the same until 03:00 pm and then declined. Interestingly, carotenoids reached a maximum level after 03:00 pm in the static open flow treatment. Irradiance had a significant effect on carotenoid fluctuations (p < 0.05), but there was no significant interaction between irradiance and water flow (Table 1).

Reactive oxygen species
ROS were measured every three hours for C. demersum in the four treatments. Of the treatments, the open flow treatment showed the highest increase in H 2 O 2 content throughout the experiment ( Figure 4A), followed by the shaded flow treatment. In addition, the static open and shaded treatments fluctuated and had lower amounts of ROS than the other flow treatments. Initially, the mean H 2 O 2 concentration was approximately 5 µmol g −1 FW for each treatment at 06:00 am. Then, the plants grown under open conditions showed a two-fold increase in H 2 O 2 at 12:00 pm, while the static open and shaded treatment values remained at 8.3 and 6.4 µmol g −1 FW, respectively. The H 2 O 2 concentration was significantly higher at mid-day than over the 12-hour sampling period (p < 0.05). Moreover, at noon, the H 2 O 2 content was significantly influenced by water flow (F = 19.762, p = 0.002) and irradiance (F = 13.248, p = 0.006), but there were no interactive effects. The H 2 O 2 concentration reached its lowest level at approximately 06:00 pm in all treatments.

Antioxidant enzymatic activities
For all treatments, POD activity followed similar daily cycles as those of the H 2 O 2 levels. POD activity increased in the first half of the day, and it declined in the latter part of the day ( Figure 4B). In comparison to the plants in static water, the plants exposed to continuous water movement had the highest POD activity. At mid-day, in all four treatments, the POD activity peaked and was significantly affected (16.54, p < 0.05) by flow (F = 303.45, p < 0.05) and irradiance (F = 71.77, p < 0.05). The mean POD activity of the open flow treatment was 10.62 ± 0.38 µmol min −1 g −1 FW at noon and was approximately two times greater than that of the static open and shaded treatments. However, POD activity declined by 30.79% in the shaded flow treatment compared to that in the open flow treatment at 12:00 pm.
The APX activity was delayed during the day and peaked at approximately 03:00 pm in all treatments. Compared to the static treatments, the flow treatments had significant effects on APX enzyme activity, which rapidly decreased at 06:00 pm ( Figure 4C). Moreover, the APX activity was relatively unchanged between 03:00 pm and 06:00 pm in the static shaded and static open treatments. However, the APX activity was reduced by 15.9% in the flow treatments compared to that in the static treatments at 06:00 pm.
The CAT activity increased along the solar irradiance gradient and peaked after mid-day at 03:00 pm in both the open and shaded flow treatments. The next highest level was observed in the static open treatment at 12:00 pm, whereas the CAT activity did not drastically fluctuate among the five sampling periods in the static shade treatment. The CAT activity was significantly influenced by irradiance in the early morning at 06:00 am and in the late evening at 06:00 pm in the four treatments, whereas significant interactive effects of irradiance and water flow were observed at noon (12.00 pm) (F = 70.54, p < 0.05) and 15:00 (F = 11.78, p < 0.05) ( Figure 4D).

Discussion
Light energy fluctuations in aquatic community production vary between 0 and 2000 µmol m −2 s −1 (Binzer and Middelboe 2005). In our experiment, the maximum PAR was 1544 µmol m −2 s −1 at midday, and the lowest value was 224 µmol m −2 s −1 ( Figure 5). The data indicate that the received irradiance quantum yield was satisfactory in terms of the general photosynthesis performance of the C. demersum population. When light energy is sufficient, it promotes photoautotrophic organism growth in an aquatic environment (Wersal and Madsen 2013). On the other hand, excess light can cause photoinhibition (Kull 2002). For C. demersum, in comparison to the other plant treatments, the plants subjected to the static open water chlorophyll a concentration were upregulated at all five sampling time points. This finding may be related to the adaptation of absorbing excessive light with chlorophyll a pigments that follows the diminishing law of returns (Frost-Christensen and Sand-Jensen 1992).  argued that an available high chlorophyll a concentration causes an increase in the photosynthesis rate of hydrophytes, and our results validated their findings. However, plants exposed to the flow with the natural irradiance chlorophyll a concentration declined. The reduction in the chlorophyll content in the plants grown in the flow treatments compared to that of the plants grown in the static treatments was related to the accumulation of ROS (Demidchik 2015) . These results indicate that C. demersum exhibited an oxidative stress response when subjected to flow movement. The consequences of flow movement-related chlorophyll reduction were discussed in previous studies (Ellawala et al. 2011;Atapaththu and Asaeda 2015). Chlorophyll b and carotenoids exhibited the same pattern as chlorophyll a among treatments. Our research findings suggest that pigment fluctuation is an adaptive strategy for coping with available irradiance. Plant species follow various adaptations to alter photosynthesis capacity, and one of these adaptations is changing pigment content (Belshe et al. 2007).
The ETR at light saturation is a good indicator of photosynthesis capacity based on fluorescence measurement  (Genty et al. 1989). In addition to the chlorophyll pigment, we observed a higher elevated ETR in the flow-exposed plants than in the other plants. This observation may be related to the boundary layer hypothesis that a reduced thickness of the boundary layer in macrophyte leaves due to water flow and enhanced uptake of CO 2 and dissolved nutrients (Wheeler 1980;Søndergaard et al. 1992) are important for photosynthesis. In addition, in comparison to that of whole-leaf plants, the morphological structure of C. demersum was identified as having a narrow-leaved, rigid shoot shape, which provides an advantage for resisting flow forces. Schutten and Davy (2000) explained that macrophyte species with narrow leaves have a high level of resistance against hydraulic forces. In both the open static and open flow treatment propagules, the ETR results revealed that photosynthesis capacity was higher than that in the shaded treatment propagules. A low photosynthesis rate under low light is a typical observation in the plant community (Biswal et al. 2012). During photosynthesis, accelerated ROS production was also high in those two treatments. This phenomenon occurs because ROS are directly generated through natural photosynthesis with increased ETR, as demonstrated by earlier studies (Rijstenbil et al. 2000;Pospíšil 2009Pospíšil , 2016. Often, a major adaptive strategy for scavenging ROS is the activation of antioxidant enzymes. Plant cell-metabolized ROS are identified as singlet oxygen, superoxide, hydroxyl radicles and H 2 O 2 that are produced through light-driven processes (Pospíšil 2016). Among all ROS, H 2 O 2 is considered a relatively stable nonradical ROS (Slesak et al. 2007). In addition, ROS react as intracellular messengers to produce cell antioxidants (Sauer et al. 2001). According to the results, we observed an excessive H 2 O 2 concentration in the bare plants in the flow treatments in comparison to the plants in the static treatment at midday, indicating that C. demersum was in a state of oxidative stress. This result may be attributed to the mechanical shear stress forced on the plant leaf tissue by water movement (Pasternak et al. 2005;, which is further aggravated by irradiance (Mittler 2002). However, excessive ROS generation signals an initiated scavenging system, and antioxidant concentrations increased drastically at mid-day. Our research findings are consistent with those of previous studies of (Atapaththu and Asaeda 2015) that explain water flow movement caused by elevated antioxidant activities in the submerged macrophyte species Elodea nuttallii.
In our study, the triggered antioxidant system was measured using three antioxidants: POD, APX and CAT. In the scavenging process, POD was considered to be a rapidly responding antioxidant (Knörzer et al. 1996). In addition, with increased solar irradiance in the morning at 0600, plants exposed to flow exhibited 1.84-fold higher POD activities than those at other times. These data indicate that POD activity in C. demersum plants tended to be upregulated with flow movement in contrast to those with static flow as a stress-responsive mechanism. Furthermore, the synthesis of POD activity markedly peaked at 12:00 pm in comparison to other sampling times under each of the four growth conditions. This finding suggests that under a natural day: night cycle, POD activity is dominant at noon to scavenge  ROS. Intensification of POD activity is more closely associated with the early response mechanism to light stress and provides cellular protection against synthesized H 2 O 2 (Zolfaghari et al. 2010). In our experiment, plants grown in the flow treatments increased their POD generation more dramatically, while plants grown under static conditions exhibited lower POD activity.
In addition to the POD activity, the APX activity plays a crucial role in the flexible antioxidant system. Asada (1992) argued that APX is a key enzyme present in the ascorbate glutathione cycle and is responsible for H 2 O 2 detoxification. Furthermore, elevated APX activity with abiotic stress has been observed in other aquatic plant species (Mishra et al. 2009;Chandani et al. 2017;Parveen et al. 2017). In our experiment, the results showed gradually elevated APX activity at approximately 03:00 pm in both flow treatments. At the end of the experimental period, the average activity declined by 51%. However, static-induced plant APX values did not sharply fluctuate and indicated a low level of oxidative stress. However, similar to the APX activity, a closely varied diurnal trend pattern was observed in the CAT activity that tended to increase in the late day time period. The delayed dominance of the CAT activity might have been related to the susceptibility of a particular enzyme to photoinactivation by excess sunlight (Shang and Feierabend 1999). These two antioxidants are responsible for transforming the H 2 O 2 substrate into H 2 O and O 2 , and this activity takes place in two separate places: peroxisomes for CAT and chloroplasts for APX (Foyer et al. 1994). Our data emphasized that the three antioxidant enzymes considered in the habitat experiment responded at different times in the daily cycle for generated H 2 O 2. CAT and APX fluctuated in the later part of the sampling period, while the POD cycle was triggered at 12:00 pm.
However, at the end of the day, continuous water flow movement caused balanced H 2 O 2 generation, where the H 2 O 2 concentration was close to both static treatment values. This finding demonstrated that perhaps an elevated response of antioxidant activities at noon resulted in a substantial recovery of ROS accumulation in the cell. The proper timing of plant responses to environmental changes is considered an adaptive advantage in plant physiology and metabolic activities (Michael et al. 2003;Dodd et al. 2005). Butow et al. (1997) explained that antioxidant enzyme regulation takes place when the total daily irradiance is aligned with the diurnal rhythm. The activation of enzymatic patterns and key genetic stimuli is not fully understood and open for further exploration. This scenario suggests that future studies should evaluate the relative importance of biological enzymatic pattern activation to oxidative stress responses.

Conclusion
The diurnal physiological conditions of the C. demersum plants significantly varied among the four growth conditions. Their ability to successfully grow in habitats was related to modulating the diurnal cycles of antioxidant activities according to flow and irradiance changes. In particular, we observed POD as an early responding antioxidant against ROS, whereas CAT and POD scavenging occurred after the POD response. Interestingly, the irradiance effect significantly elevated oxidative stress at noon; however, with the activated antioxidant system, the effect was mediated a considerable amount in the late evening. Moreover, among the four treatments, the static open conditions can be considered the most favorable for the growth of this macrophyte. The lower level of H 2 O 2 concentration coupled with the highest photosynthesis performance occurred in the plants under static open conditions. This result implies that a healthy cell environment for optimum plant growth may facilitate the invasive potential of the species. Additionally, in comparison to unshaded habitats, shaded habitats tend to decrease photosynthesis capacity. If these habitats exist, then those places may be vulnerable to invasion by these species. Therefore, ecologists should consider managing aquatic ecosystems to control this species. Further studies should focus on understanding the gene-level regulators that control these diurnal activities.

Disclosure statement
No potential conflict of interest was reported by the authors.

Funding
This research was financially supported by a Research Grant in Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

Notes on contributors
Viraj Prasanna Ranawakage is a PhD student at Department of Environmental Science, Saitama University Japan with research interest in fresh water macrophytes.
Takashi Asaeda is a professor emeritus at Saitama University Japan. His research interests have broadly covered ecology, hydrology, and fluid mechanics in the environment, including physical processes of watered area, structure of urban heat island and field observation and mathematical modeling of aquatic ecology and biology.