Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract

Abstract Type 2 diabetes (T2D) is a progressive metabolic disorder of glucose metabolism. One of the therapeutic approaches for the treatment of T2D is reducing postprandial hyperglycaemia through inhibition of the digestive enzymes α-glucosidase and α-amylase. In this context, aimed at identifying natural products endowed with anti-T2D potential, we focused on Ptilostemon casabonae (L.) Greuter, a species belonging to Asteraceae family. Enzymatic inhibition, antioxidant activity, phenolic composition and cellular assays were performed. This study revealed that the P. casabonae hydroalcoholic extract exerts a potent inhibitory activity against α-glucosidase. This activity is supported by an antioxidant effect, preventing ROS formation in a stressed cellular system. HPLC-PDA-MS/MS analysis, revealed a complex polyphenolic fraction. Among the tested pure compounds, 1,5-dicaffeoylquinic acid, apigenin and rutin displayed good α-glucosidase inhibitory activity. Our study suggested new potential of P. casabonae encouraging us to further testing the possible therapeutic potential of this extract. Graphical Abstract


Introduction
Ptilostemon casabonae (L.) Greuter is a wild edible species belonging to the Asteraceae family, subendemic to Sardinia (Italy), Corsica and Hy� eres Island (France) 1 .It is a spiny herb, up to 150 cm in height, with linear-lanceolate leaves provided with thorns bundles in all the leaf edges and purple inflorescences appearing from May to August.It grows in dry and open habitats, indifferently in calcareous or siliceous soils, between 400 and 1200 m a.s.l.Aerial parts of P. casabonae are used in Sardinian traditional medicine as anti-spasmodic 2 .
Even though many Sardinian endemic species have been reported as source of unique and structurally diverse compounds endowed with promising biological activities 3,4 , the majority of them still remain understudied 5 .
Previous studies focused on the phytochemical analysis of the extracts obtained from its aerial parts, providing information on its primary and specialised metabolites.A remarkable amount of phenolic and amino compounds has been reported 6,7 , making this plant a good candidate for food supplements and functional foods.However, as far as we know, more information on the biological activities of this species is needed.These considerations encouraged us to investigate its antioxidant and antidiabetic potential.
Diabetes is a progressive metabolic disorder of glucose metabolism.It represents a global public health issue, affecting 537 million adults (20-79 years) worldwide, with this number expected to rise to 783 million by 2045 8 .Moreover, 45% of people live with undiagnosed diabetes, predominantly type 2, so the incidence of the disease should be considered even higher.
Type 1 diabetes is caused by impaired insulin production by pancreatic b-cells and a daily insulin injection is required to keep blood glucose level within an appropriate range.Type 2 diabetes (T2D) is the most prevalent type of diabetes, representing more than 90% of all diabetes worldwide, and it is mainly characterised by insulin resistance or b-cell dysfunction 9 .The causes of T2D are not exactly understood.Still, it is known that the disease onset is closely associated with various risk factors such as overweight and obesity, increasing age, ethnicity, and family history.Strategies for T2D management are oriented towards a correct lifestyle, including a healthynutrition, regular physical activity, quitting smoking, and maintenance of healthy body weight.However, oral medication is needed if this approach is insufficient to control blood glucose level.The treatment can include a single antidiabetic medication, but many combination therapy options are often used.Several drugs with different modes of action are available, including the a-glucosidase inhibitors.
a-Glucosidase (EC 3.2.1.3)and a-amylase (EC 3.2.1.1)enzymes can hydrolyse carbohydrates and therefore represent important therapeutic targets in controlling postprandial hyperglycaemia in diabetic disease 10 .Salivary and pancreatic a-amylases catalyse the endohydrolysis of a-D-1,4-glycosidic bonds in starch, producing maltose and other oligosaccharides, while intestinal a-glucosidase catalyses the hydrolysis of terminal 1,4-linked a-D-glucose residues from nonreducing ends of isomaltose oligosaccharides, yielding free D-glucose, which can be absorbed by intestinal cells.Inhibitors of these enzymes slow down carbohydrate digestion, thus prolonging overall digestion time, causing a reduction in glucose absorption, thereby moderating the postprandial blood glucose elevation.Therefore, a therapeutic approach to treat diabetes is to inhibit these enzymes to decrease postprandial hyperglycaemia.
Moreover, it is well known that there is a strong link between hyperglycaemia, hyperglycaemic-induced oxidative stress and the development and progression of T2D.Oxidative stress stimulates the generation of inflammatory mediators, and inflammation, in turn, improves the production of reactive oxygen species (ROS) that can lead to many diseases, including T2D.ROS accumulation can promote cellular damage and contribute to diabetic complications such as cardiovascular alterations 11 .Therefore, therapies based on the downregulation of ROS generation may be pivotal in treating T2D and controlling diabetic complications.
Thus, in this context, the present study aims to obtain and characterise a novel potent antidiabetic extract from P. casabonae with concomitant antioxidant activity.

Plant material
Leaves of P. casabonae were collected from Jerzu (Sardinia, Italy) in May 2021.The plant was identified by Cinzia Sanna, and a sample was deposited at the General Herbarium (HerbariumCAG) of the University of Cagliari (Cagliari, Italy), with the voucher specimen (CAG 796/v1).The plant, even though endemic, is not under the protection of national or international regulations; thus, it was not necessary a specific permission for its harvesting.Leaves were dehydrated at 40 � C by using a ventilated stove, and powdered by an electric grinder.

Extract preparation
Powdered leaves of P. casabonae (32 g) were subjected to three consecutive and exhaustive extractions with 80% ethanol (800 ml � 3) for 24 h each.Extracts were then pooled, filtered, and concentrated under vacuum at 40 � C to eliminate the alcohol phase.The remaining aqueous phase was frozen to −80 � C and then lyophilised, yielding 3.7 g of crude extract that was kept at −20 � C until chemical analyses and biological assays.Before use, unless otherwise specified, the extract was dissolved in water at the desired concentration for chemical and biological assays.

Characterisation of the extract through HPLC-PDA-MS/MS
A Shimadzu Nexera � 2 system coupled with an SPD-M20A photodiode array detector and a Shimadzu LCMS-8040 triple quadrupole system with electrospray ionisation source (Shimadzu, Dusseldorf Germany) was used for the qualitative and quantitative analyses of the extract, as previously reported by Marengo et al. 6 .Analytical conditions are: column AscentisV R Express C18 (15 cm � 2.1 mm, 2.7 lm, Supelco, Bellefonte, USA); mobile phases: A: water (added with 0.1% formic acid), B: acetonitrile (added with 0.1% formic acid); flow rate: 0.4 ml/min; UV spectra: 220-600 nm; column T: 30 � C; Gradient program: 5% B for 5 min, 5-15% B in 17 min, 15-25% B in 10 min, 25-75% B in 12 min, 75-100% B in 10 min, 100% B for 1 min.Total time (pre-and post-run) was 60 min.MS operative conditions: Heat block temperature 200 � C; desolvation line (DL) temperature: 250 � C; nebuliser gas flow rate: 3 L/min; drying gas flow rate: 15 L/min.Mass spectra were acquired in both positive and in negative full-scan modes (range 100-1000 m/z, event time 0.5 s).Product Ion Scan mode: collision energy: ESIþ: − 35.0 V and ESI−: 35.0 V, event time: 0.2 s.Quantification was performed in the UV, with each compound quantified by the external calibration method using the relative commercial standard compound at its maximum absorption (325 nm for chlorogenic and 1,5 dicaffeoylquinic acid; 350 nm for rutin; 340 nm for apigenin).Succinyl dicaffeoylquinic acid, for which no commercial standard was available, was quantified using 1,5-dicaffeoylquinic acid calibration curve.For the standard solutions and the extracts three replicates of analyses were performed.

SPE-C18 cartridge purification
Succinyl dicaffeoylquinic acid-enriched fraction of P. casabonae leaves extract was prepared on an SPE-C18 cartridge.A conditioning of the cartridge was first carried out with 4 ml of methanol and 4 ml of deionised water.10 mg of the dried extract (DE) was resuspended in 1 ml of water, loaded onto the Bond Elut Jr 500 mg SPE-C18 cartridge, and eluted with 5 ml of deionised water.The fraction was then freeze-dried, yielding approximately 2 mg.This procedure was repeated to obtain a sufficient amount of the fraction for the biological tests.The fraction obtained was analysed by HPLC-PDA-MS/MS for its chemical characterisation and quantification of the major constituents.

Fatty acid analysis
Aliquots (2 mg, in ethanol solution) of DE were subjected to mild saponification as previously reported 12 .Dried saponifiable fractions, dissolved in acetonitrile, were analysed by high-performance liquid chromatography (HPLC).Fatty acids (FA) analyses were performed after saponification, and carried out with an Agilent Technologies 1100 HPLC (Agilent Technologies, Palo Alto, CA) equipped with a DAD and an Infinity 1260 ELSD detector (HPLC-DAD/ELSD).FA were eluted with CH3CN/H2O/CH3COOH (75/25/ 0.12, v/v/v) as mobile phase at a flow rate of 2.3 ml/min, using a XDB-C18 Eclipse column 12 .Saturated FA (SFA) were detected using ELSD, while unsaturated FA (UFA) at wavelength of 200 nm (DAD).Chromatogram data recording and integration were carried out through an Agilent OpenLAB Chromatography data system.The FA identification was performed using standard compounds and UV spectra (for UFA).FA calibration curves were constructed using the reference standards and were found to be linear (DAD) and quadratic (ELSD) (correlation coefficients >0.995) 12 .

Determination of the total phenolics and flavonoids
The total phenolic content in the extract was determined by using the method of Folin-Ciocalteu, using gallic acid as a reference standard, while aluminium nitrate assay was used to determine the total flavonoid content 13 .Phenolic and flavonoid concentration was expressed as mg of gallic acid equivalents (GAE) or mg of quercetin equivalents (QE) per g of DE, respectively.

ABTS radical scavenging activity
2,2 0 -Azinobis-(3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS) radicalscavenging activity was determined with the method as previously reported 13 , using Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2carboxylic acid) as the standard reference.Briefly, the reaction between the reduced ABTS (7 mM) with potassium persulfate (2.45 mM) in an aqueous solution at room temperature for 24 h, was performed to produce free radical ABTS .þ .Then, different concentrations of extract (10 lL) were added to 1 ml of ABTS .þ, and the absorbance at 734 nm was recorded after 1 min.The activity was expressed as the concentration of sample necessary to give a 50% reduction in the original absorbance (EC 50 ).

FRAP assay
Ferric reducing antioxidant power assay (FRAP) was conducted as previously described 14 , with slight modifications.An amount of 10 mL of the extract was mixed with 2 ml of the TPTZ-ferric solution and than incubated for 4 min at room temperature in the dark.The absorbance was read at 593 nm and the result was expressed as ferrous equivalents (FE; mg Fe 2þ equivalents/mL solution).Ascorbic acid was used as reference compound.

Enzyme inhibition
The results of the catalytic activities were reported as the percentage of the blank control.The concentrations of extract required to give 50% inhibition of enzyme activity (IC 50 ) were determined.Acarbose was used as a reference compound for both enzymes.The Lineweaver-Burk double reciprocal plot was used to determine the mode of a-glucosidase inhibition by performing assays at different concentrations of substrate and extract or compound.Grafit 7 (Erithacus, East Grinstead, UK) was used as data analysis software.
a-Glucosidase inhibition assay a-Glucosidase inhibitory activity was analysed following the method described by Delogu et al. 15 .The reaction mixture consisted of 120 lL of 0.1 M sodium phosphate buffer (pH 6.8), 40 lL of Saccharomyces cerevisiae a-glucosidase (0.125 U/mL) and 20 lL extract or single compound at different concentrations.It was first incubated for 15 min at 37 � C.Then, 20 mL of p-nitrophenyl a-Dglucopyranoside solution (pNPG, 5 mM) was added.After further 15 min of incubation at 37 � C, the reaction was stopped by adding 50 lL of 0.2 M sodium carbonate solution.a-Glucosidase activity was determined with a 96-well microplate reader by measuring the absorbance at 405 nm.The mode of inhibition was determined, and the equilibrium constant for binding with the enzyme-substrate complex (K IS ) was obtained from the vertical intercepts plotted versus inhibitor concentration.

a-Amylase inhibition assay
The reaction mix used to determine the a-amylase activity consisted of 60 lL of sodium phosphate buffer (50 mM, pH 7.0), 20 lL of NaCl (1 M) and 40 lL of a-amylase from porcine pancreas (1 mg/mL).The solution was incubated at 37 � C for 10 min in the absence or presence of extract or a single compound.After incubation, 80 lL of the 2-chloro-4-nitrophenyl-a-D-maltotrioside substrate solution (CNPG3, 2.5 mM) were added, and the amount of 2-chloro-nitrophenol released by the enzymatic hydrolysis was consequently monitored at 405 nm 15 .
Caco-2 cells were also treated with the same concentrations of extract to evaluate the cellular ROS levels by using the 2 0 ,7 0dichlorofluorescein diacetate (DCFH-DA) method.After 24 h, cells were incubated with DCFH-DA (10 lM) at 37 � C for 30 min and then treated with 1 mM H 2 O 2 .The fluorescence intensity of DCF was immediately measured with a fluorescent plate reader at the excitation wavelength of 485 nm and emission wavelength of 530 nm.Readings were taken every 5 min for 60 min 14 .

Statistical analysis
All assays were conducted in triplicates and the data were expressed as mean ± standard deviation (SD).GraphPad Prism software version 9 (San Diego, CA, USA) was used to evaluate statistical differences.Comparison between groups was performed by one-way analysis of variance (ANOVA) followed by the Tukey Multiple Comparisons Test.A p values of less than 0.05 was considered statistically significant.

Total polyphenols, total flavonoids content and antioxidant activity
Polyphenols and flavonoids are important bioactive compounds endowed with strong antioxidant properties 16,17 .In this work, we first evaluated the total phenolic and flavonoid content of a hydroalcoholic extract obtained from P. casabonae leaves.Then, we investigated its radical scavenging activities by the ABTS assay.The results obtained, shown in Table 1, revealed a rich phenolic fraction since the total phenolic content was found to be 321.31± 4.06 mg GAE/g of DE, and the flavonoid content was found 214.40 ± 3.67 mg QE/g of DE.Regarding the antioxidant activity, the extract displayed an EC 50 value of 10.46 ± 0.26 mg/mL, close to that of Trolox, used as a standard.This result was also confirmed by FRAP assay that showed a strong reducing power of P. casabonae extract.

Fatty acid composition
Since unsaturated fatty acids are described as competitive inhibitors of starch digestive enzymes (a-amylase and a-glucosidase) 18 , the fatty acid composition of the extract was performed.
Figure 1 shows the representative chromatographic profile of the main fatty acids (FA) in the leaves extract of P. casabonae (Figure 1(A)) obtained by HPLC-DAD/ELSD analysis 12 .The combined use of the two detectors allowed to quantify both saturated (SFA, ELSD detection) and unsaturated (UFA, UV detection) FA in one single analysis 12 .The most represented FA of the extract were palmitic acid (16:0, 2.49 ± 0.42 mg/g of dry weight), oleic acid (18:1 n-9, 1.36 ± 0.5 mg/g of dry weight), linolenic acid (18:3 n-3, 1.08 ± 0.29 mg/g of dry weight), and linoleic acid (18:2 n-6, 0.47 ± 0.14 mg/g of DE) (Figure 1(B)).The amount of lipids in the hydroalcoholic leaves extract of P. casabonae was very low, and the total FA content approximately accounted for 0.54% of the extract.
The FA profile of P. casabonae leaves extract was different from that reported for the fruits of Ptilostemon afer (Jacq.)Greuter subsp.eburneus Greuter, characterised by a high content (expressed as %, DE) of linoleic acid (58.5 ± 0.1), followed by oleic acid (19.2 ± 0.1), and palmitic acid (9.0 ± 0.1) 19 .The P. casabonae FA profile was similar to those previously reported for lipid extracts obtained from the leaves of various medicinal and edible plants 20 , characterised by palmitic acid as the dominant FA, followed by oleic, linolenic, and linoleic acids in different proportions.
Our results on lipid content add useful information to the chemical characterisation of this understudied subendemic plant, including its primary metabolites.These compounds, together with specialised metabolites, are very important for their nutritional and healthy properties, especially considering that this species, as well as the other wild thistles, are traditionally used for food.As far as we know, this is the first report on lipid content of P. casabonae leaves, while a previous article investigated the content of amino compounds, revealing proline, asparagine, glutamine and glutamic acid as the main constituents in the acidified hydroalcoholic extract of the aerial parts of this species 7 .

a-Glucosidase and a-amylase inhibition of P. casabonae leaves extract
Table 2 reports the ability of the extract of P. casabonae leaves to reduce a-amylase and a-glucosidase activities.The extract weakly  inhibits a-amylase, while it exhibits potent inhibitory activity against a-glucosidase (Table 2).Indeed, P. casabonae extract was found to be about 7 times more active (p < 0.001) than the standard inhibitor, acarbose, with an IC 50 value of 12.20 ± 0.99 mg/mL.Acarbose is commonly used to treat hyperglycaemia in T2D patients; however, the use of this drug is accompanied by several side effects, probably due to a greater inhibition of a-amylase in comparison to a-glucosidase inhibition 21 .P. casabonae extract instead showed a better inhibition against a-glucosidase, making this extract a good candidate for further and more detailed studies.
The Lineweaver-Burk plots showed that the extract behaves with an uncompetitive mode of inhibition since the kinetic analysis of the extract gives a family of parallel lines for increasing extract concentrations (Figure 2).The equilibrium constant for binding with the enzyme-substrate complex (K IS ) was calculated from the replotting of the intercepts (1/V max ) versus the inhibitor concentration, resulting in a value of 13.9 lg/mL.

Cell viability and intracellular ROS level
Since the extract of P. casabonae exhibited a good antioxidant activity on ABTS assay, we decided to investigate whether it exerts antioxidant effects also by inhibiting H 2 O 2 -induced ROS generation in a cellular model.
The effect of the extract was first investigated on cell viability using Caco-2 as cell line.Caco-2 cells were exposed for 24 h to various concentrations of the extract and then examined with the MTT test.As shown in Figure 3, the extract was not cytotoxic, and only a little decrease (viability of 85%) was observed at 100 lg/mL.
Since the viability was not affected up to a concentration of 100 lg/mL, we performed further cellular experiments using up to this extract concentration.To investigate the protective effects against oxidative stress, we evaluated the intracellular ROS levels before and after H 2 O 2 -induced oxidative stress and after incubation with the extract.The experiment was performed using DCFHDA, which is hydrolysed by the endogenous esterases to DCFH.The oxidation of DCFH to DCF, induced by H 2 O 2 , was quantified through a spectrophotometer.As shown in Figure 4, the incubation with H 2 O 2 significantly increased ROS levels in Caco-2 cells, but treatment with P. casabonae extract inhibited H 2 O 2induced ROS generation in a dose-dependent manner.These results confirm what observed in the antioxidant assays, and suggest a potential role of P. casabonae extract in reducing ROS formation in cells.At the concentration of 100 lg/mL, ROS formation was reduced back to non-treated cells level, since no statistically differences were observed between the two samples.At the same concentration, P. casabonae extract exerts almost totally inhibition against a-glucosidase activity, with a concomitant effect against a-amylase (Table 2), also showing no cytotoxic effect (Figure 3).

Phytochemical study of P. casabonae extract
Based on the interesting antioxidant and potential antidiabetic properties observed, the characterisation of the extract of P. casabonae leaves was carried out by HPLC-PDA-MS/MS to identify the main compounds which could be responsible for the observed activity.The phytochemical analysis, in agreement with the total phenolic and flavonoid content results described above, revealed   a complex polyphenolic fraction.Our results are in line with a previous study in which caffeoylquinic acid derivatives are described as the most representative group of specialised metabolites detected in this species 6 .As shown in Figure 5, twenty-four peaks were detected, including quercetin, luteolin, kaempferol, apigenin and diosmetin O-glycosides, in addition to the caffeoylquinic acid derivatives.
The list of all compounds detected in the extract is reported in Table 3.Among these, fourteen were confirmed by the coinjection of commercial standards.For compounds for which no reference standards were available, the putative identification was performed by comparison with literature data, considering their maximum UV absorbance, the molecular weight based on the pseudomolecular ions detected in the positive and negative ioniza-tion modes and the characteristic fragments obtained by tandem mass spectrometry.
All compounds, except for coumaroylquinic acid (2), isorhamnetin-O-glucoside-rhamnoside (10), quercetin O-hexoside (14), di-O-p-coumaroyl quinic acid (18), and succinyl di-p-coumaroylquinic acid isomers (20 and 21) have been previously found in this species 6 .The phytochemical profile of P. casabonae was also found to be similar to that described for other wild thistles 14,25,26 .The most representative specialised metabolites belong to the class of hydroxycinnamates, with succinyl-dicaffeoylquinic acid (12) and 1,5-dicaffeoylquinic acid (9) amounting to 99.82 ± 3.23 and 98.64 ± 1.50 mg/g of DE, respectively.These compounds, together with other flavonoids and phenolic acids (chlorogenic acid (1) 21.94 ± 0.12 mg/g of DE, rutin (4) 15.65 ± 0.68 mg/g of DE and apigenin (23) 0.15 ± 0.006 mg/g of DE) have been tested to verify their role in the activity observed.For all the above-mentioned compounds, authentic commercial standards were used for the biological assays.An exception was made for succinyl-dicaffeoylquinic acid (12) for which an enriched fraction was prepared as this standard is not commercially available.
As shown in Figure 6, this fraction was composed of succinyldicaffeoylquinic acid (43.75 mg/g of fraction), chlorogenic acid (34.9 mg/g of fraction), and 1.5 dicaffeoylquinic acid (in traces).The fractionation carried out did not allow a complete purification of succinyl-dicaffeoylquinic acid due to the chemical similarity of the components characteristic of the extract.

a-Glucosidase inhibition of P. casabonae compounds
The inhibitory effect of the single compounds as well as the fraction enriched in succinyl-dicaffeoylquinic acid against a-glucosidase activity was evaluated (Table 4).
The results show that P. casabonae fraction enriched in succinyl-dicaffeoylquinic acid was inactive towards a-glucosidase.This fraction mainly consists of succinyl-dicaffeoylquinic acid and chlorogenic acid and a tiny amount of 1,5-dicaffeoylquinic acid.
Considering that chlorogenic acid has no inhibitory activity against a-glucosidase (Table 4) and the amount of 1,5-dicaffeoylquinic acid present in the fraction is not sufficient to exert an enzyme inhibition, it can also be affirmed that succinyl-dicaffeoylquinic acid does not possess inhibitory potential towards a-glucosidase.Our results showed that 1,5-dicaffeoylquinic acid, apigenin and rutin displayed good inhibitory activity, with IC 50 values ranging between 3.88 ± 1.48 mg/mL and 36.64 ± 0.07 mg/mL, all significantly lower (p < 0.001) than the standard inhibitor acarbose (Table 4).1,5-Dicaffeoylquinic acid is a compound present in some edible plants (artichoke and sunflower sprouts).Other caffeoylquinic acids detected in our extract, such as 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid have been previously found able to inhibit this enzyme 27 , suggesting that the significant inhibition of the P. casabonae extract against a-glucosidase enzyme, observed in our study, could be attributable to a synergistic effect between several compounds.The inhibitory activity exhibited by apigenin and rutin support this hypothesis.
The mode of inhibition of the active compounds was also performed and revealed that 1,5-dicaffeoylquinic acid acts as an uncompetitive inhibitor; in fact, the increased concentration of the compound gave rise to a family of parallel straight lines (Figure 7).The constant for binding with enzyme-substrate complex (K IS ) was calculated from 1/V max versus inhibitor concentration and resulted in a value of 69.4 mg/mL (Figure S1).
Instead, both apigenin and rutin exerted a mixed-type inhibition since increasing the concentration of compounds resulted in a family of straight lines with different slopes and y-intercepts, which intersected in the second quadrant.This kinetic analysis indicates that they can bind not only with the free enzyme but also with the enzyme-substrate complex (Figure 7).The equilibrium constants of rutin and apigenin for binding with the enzyme-substrate complex (K IS ) were found to be 80.2 mg/mL and 19.6 mg/mL respectively, while the equilibrium constants for binding with the free enzyme (K I ) were obtained from the slope (Km/ Vmax) versus inhibitor concentration and resulted in a value of 9.3 mg/mL and 6.5 mg/mL respectively (Figure S1).

Conclusions
Natural plant products offer unlimited potential for new drugs because their chemical diversity could result in a range of biological activities.P. casabonae was revealed to possess a complex polyphenolic composition mainly characterised by caffeoylquinic acid derivatives, followed by flavonoids.Polyphenols and flavonoids are considered important bioactive compounds with strong antioxidant properties.Therefore, radical scavenging activities of P. casabonae leaves were investigated, and the result showed good antioxidant activity, also confirmed by the reduction of ROS formation performed on cellular experiments.
It is well known that the production of free radicals and ROS play a significant role in the development of oxidative stress, which is believed to be related to increasing hyperglycaemia, which is associated with T2D.In this respect, P. casabonae exhibited potent inhibitory activity against a-glucosidase, being even better than the standard inhibitor, along with a good antioxidant activity.These results suggest that P. casabonae could be a promising candidate to provide natural bioactive compounds for treating T2D.Encouraged by the results obtained, further experiments are ongoing in order to evaluate the in vivo effect and develop a nanoformulation delivery system of this extract with the aim of exploiting and possibly enhancing its bioactivity for application in T2D therapy.

13 Figure 1 .
Figure 1.Fatty acid (FA) chromatographic profiles (HPLC analysis) obtained by DAD (200 nm, unsaturated FA) and ELSD (saturated FA) detection (A) and FA composition (expressed as mg/g of extract) determined by HPLC-DAD/ELSD analysis (B) of P. casabonae leaf extract after saponification.Analysis was performed in quadruplicate and all data are expressed as mean values ± standard deviations (SD) (n ¼ 4).

Figure 2 .
Figure 2. Inhibition of a-glucosidase activity.Lineweaver-Burk analysis (A) and secondary plot (B) using different concentrations of the extract.

Figure 3 .
Figure 3.Effect of P. casabonae extract on Caco-2 cell viability after 24 h incubation with the extract at different concentrations.No statistically differences between treated and non-treated (NT) cells were observed.

Figure 4 .
Figure 4. Inhibition of H 2 O 2 -induced ROS generation (1 h-incubation with 2 mM H 2 O 2 ) by P. casabonae extract on Caco-2 cells.NT, non-treated cells; T, cells treated with H 2 O 2 only.Asterisks indicate values statistically different ( � p < 0.05; �� p < 0.0001) from cells treated with H 2 O 2 only (T).All H 2 O 2 -treated samples were statistically different from NT with the exceptions of 100 lg/mL P. casabonae extract.
peak is described by its relative retention time, UV absorption maximum, pseudomolecular ions, molecular weight (MW), fragments obtained by Product Ion Scan mode (PIS) and compound name.References are also included.a Identification confidence value: Level a: Identified compound through the co-injection of an authentic reference standard; Level b: Hypothesise compound (similarity of chromatographic and spectral data to published data); Level c: Unknown.

Figure 7 .
Figure 7. Inhibition of a-glucosidase activity by single compounds.Lineweaver-Burk plot for inhibition of a-glucosidase at different compounds concentrations.

Table 1 .
Evaluation of antioxidant activity, total phenolic and flavonoid content of P. casabonae leaves extract.

Table 2 .
Inhibitory activity of P. casabonae extract against a-glucosidase and a-amylase.

Table 4 .
IC 50 value (lg/mL) and inhibition type of P. casabonae fraction and single compounds.