Anti-breast cancer action of carbonic anhydrase IX inhibitor 4-[4-(4-Benzo[1,3]dioxol-5-ylmethyl-piperazin-1-yl)-benzylidene-hydrazinocarbonyl]-benzenesulfonamide (BSM-0004): in vitro and in vivo studies

Abstract Anti-breast cancer action of novel human carbonic anhydrase IX (hCA IX) inhibitor BSM-0004 has been investigated using in vitro and in vivo models of breast cancer. BSM-0004 was found to be a potent and selective hCA IX inhibitor with a Ki value of 96 nM. In vitro anticancer effect of BSM-0004 was analysed against MCF 7 and MDA-MA-231 cells, BSM-0004 exerted an effective cytotoxic effect under normoxic and hypoxic conditions, inducing apoptosis in MCF 7 cells. Additionally, this compound significantly regulates the expression of crucial biomarkers associated with apoptosis. The investigation was extended to confirm the efficacy of this hCA IX inhibitor against in vivo model of breast cancer. The results specified that the treatment of BSM-0004 displayed an effective in vivo anticancer effect, reducing tumour growth in a xenograft cancer model. Hence, our investigation delivers an effective anti-breast cancer agent that engenders the anticancer effect by inhibiting hCA IX.


Introduction
Breast cancer is the most prevalent type of cancer that affects millions of people globally and is correlated with the main cause of mortality in women aged between 45 and 55 years 1,2 . It is a type of tissue cancer that mainly arises from the inner layer of lobules and ducts. Breast cancer is a multistage disease and it reaches the metastasis stage in advanced cases 3,4 . The main risk factors of this disease include age, high hormone level, bad lifestyle, iodine deficiency, and economic status 5,6 . The therapy of breast cancer mainly relies on tissue removal, hormone therapy, and chemotherapy 7,8 . In the past decades, enough progress has been made for successful therapy of breast cancer. Unfortunately, several cases of breast cancer are still incurable, especially advanced-stage breast cancer 9,10 .
Among 15 isoforms of CAs, CA IX is extensively studied as an effective target for the therapy of hypoxic cancers [19][20][21][22][23] . Human carbonic anhydrase IX (hCA IX) has 459 amino acid residues, consisting of an extracellular part, an N-terminal signal peptide, a transmembrane region, and an intracellular part. The extracellular part contains a catalytic domain, situated nearby the plasma membrane (displaying high sequence homology with other hCA isoforms) and a proteoglycan (PG)-like domain that is considered as a unique property of CA IX, it is found to be absent in other isoforms 19,[24][25][26] . CA IX gene is associated with hypoxic tumour cells and codes a transmembrane glycoprotein consisting of an extracellular catalytic domain as well as N-terminal PG domain, regulated by hypoxia-inducible factor-1 (HIF-1) 27,28 . CA IX is widely expressed in various types of solid tumours and promotes the progression of tumours as well as their metastasis by generating an acidic environment in tumour cells [29][30][31] . Under normal physiological conditions, very limited expression of CA IX was observed and found mainly in intestine and gallbladder epithelia 32,33 . However, overexpression of hCA IX has been observed in a variety of malignant cancers such as breast, head and neck, renal, colon, lung, oesophagus, ovary, vulva, and brain tumors 20,[34][35][36][37] . Thus, CAs actively participate in maintaining physiological pH in cancer cells and acidic pH in the extracellular part that are considered as a favourable environment for the proliferation of cancer cells and their metastasis 38,39 . It has been found that the catalytic domain of hCA IX that is present on the extracellular part of the cell is actively involved in this process, maintaining the concentration of proton and bicarbonate 40,41 . It has been studied that CA IX promotes acidic pH of tumour cells in a culture medium and its inhibition decreases survival of cancer cells under hypoxia condition 42,43 . The function of CA IX in cancer cell progression and migration is also correlated with the Rho/ROCK signalling pathway 44 . The investigations also directed that CAIX may influence cell adhesion pathways to promote cancer cell survival and proliferation 45 . Thus, hCA IX emerged as an effective druggable target to control cancer cell proliferation and metastasis 19 . In order to target hCA IX for the management of several cancers, numerous varieties of small molecules have been developed as effective hCA IX inhibitors 19,46 . Among all categories, sulphonamides emerged as a very effective class of hCA IX inhibitors 47,48 . Over the last decades, several sulphonamides containing hCA IX inhibitors have been developed, with excellent in vitro and in vivo anticancer activity 19 . Interestingly, ureido-benzenesulfonamide hCA IX inhibitor SLC-0111 emerged as an effective anticancer agent, showing efficacy against various types of cancers and is currently in phase Ib/II clinical trials 49 . However, most other hCA IX inhibitors with effective anti-cancer activity are under the preclinical evaluation stage and very few hCA IX inhibitors are running in the clinical trial study which may hamper the progress of hCA IX targeted anti-cancer drug development 19,50 . Therefore, effective hCA IX inhibitors with promising anticancer activity are needed to explore this target for the management of other types of cancer. Recently, we have developed benzene sulphonamide based hCAs inhibitors using substituted piperazine tails with benzylidenehydrazine carbonyl spacer. Among them, 4-[4-(4-Benzo [1,3]dioxol-5-ylmethyl-piperazin-1-yl)-benzylidene-hydrazinocarbonyl]-benzenesulfonamide (BSM-0004) appeared to be an effective and selective inhibitor for hCA IX 13 . In the present work, the anti-breast cancer activity of BSM-0004 has been carried out against MCF7 and MDA-MA-23 cells under normoxic as well as hypoxic conditions. In order to explore the molecular mechanism of this novel hCA IX inhibitor, several in vitro experiments have been performed. Additionally, in vivo anti-breast cancer activity of BSM-0004 has been assessed, using xenograft nude mice model.

Cell line and culture conditions
MCF-7 cells were procured from ATCC (Manassas, VA) and maintained in complete DMEM media. Approximately, 10% foetal bovine serum (FBS; Hyclone, Logan, UT) and 1% antimycotic and antibiotic (Cat. No. 15240-06; Gibco, Gaithersburg, MD) were added to prepare complete DMEM medium. The cells were grown in an undeviating system of 95% air, and 5% CO 2 at the 37 C.

Cytotoxicity and proliferation assay
Cytotoxic effect of BSM-0004 against breast cancer MCF7 and MDA-MA-231 cells was investigated using 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assays. Totally, 1.0 Â 10 5 cells were seeded in 96-well plates for overnight incubation with complete DMEM medium and subjected to the experimental treatments as indicated. Twenty-four hours postdrug treatments, the viable cells were stained by adding 25 lL of freshly prepared MTT solution (5 mg/mL in PBS) in to individual wells (containing 100 lL cell culture media) and incubated at 37 C for 4 h. After incubating for 4 h at 37 C, the media were removed and 150 lL DMSO was added to dissolve the formazan. Following 30 min incubation at room temperature in a humidified chamber, colourimetric measurements were done using a multiwell microplate reader (LEDETECT96 LED based 8 channel Microplate Absorbance Reader). Efficacy of BSM-0004 and morphological analysis were also observed by optical microscope and captured cells morphology images in a different dose 51-54 .

Colony formation assay
About 500 cells resuspended in DMEM complete medium containing 10% FBS were plated on each well of six-well plates. The plates were incubated with or without 200 mM CoCl 2 and treated with 10 and 20 mM BSM-0004 and propagated under the steady state conditioned with 37 C in a 5% CO 2 incubator for nine days. The total number of colonies was stained with crystal violet dye solution for 2 h. The stained colonies greater than 20 lm in diameter were counted using the ImageJ software (Bethesda, MD).

Early and late apoptosis analysis
MCF7 cells were thawed and cultured overnight, next day the cells were collected using trypsin-EDTA (0.25% Thermo Fisher Scientific Co., Seoul, Korea), then mixed with 3 mL of DMEM to seed in 60 mm cell-culturing round petri-dishes for 24 h. Next day, cells were treated with 10 and 20 mM of BSM-0004 with or without 200 mM of CoCl 2 . Followed by incubation with 24 h, total 1 Â 10 6 cells from each sample were collected and washed with cold PBS. In order to analyse total apoptotic cells in each sample, 5 lL of Annexin V-FITC and propidium iodide (PI) (Kit, AntGene, ant003, Wuhan, China) were added and mixed properly then incubated at RT for 30 min. Before analysis, 500 lL 1Â binding buffer was added then cells were quantified for apoptosis using BD FACSCanto TM II Cell Analyser (Becton-Dickinson, San Jose, CA).

Flow cytometric analysis for cell cycle arrest
The sub-population of cells in various cell cycle phase was investigated by staining with PI. The cell checkpoints were observed using BD FACSCanto TM system according to reported previously [51][52][53][54] . Following to the treatment with 10 and 20 mM (with or without 200 mM CoCl 2 ) concentrations of BSM-0004 for 24 h, approximately $1 Â 10 6 cells MCF7 cells were harvested and fixed with 70% ethanol at room temperature by gently vortexed and kept at 4 C for overnight. The fixed cells were rinsed two times with PBS. The PI-staining has been carried out to detect the percentages of cells in different checkpoints as G1, S, and G2/M phases of the cell cycle and the signals were detected using FL2-channel.

Protein extraction and western blot analysis
For the protein estimation, MCF7 cells were cultured in DMEM complete medium with 10 and 20 mM concentration of BSM-0004 and DMSO (control group), respectively. Next day, cells were harvested using pellet downed by centrifugation at 1000 rpm (Hanil Scientific, Inc., Gimpo, South Korea) for two minutes at 4 C. Then, cells were rinsed with 1Â PBS and followed the instruction as reported previously [51][52][53][54] . Briefly, cells pellet was spin-down and lysed in the RIPA lysis buffer with DTT, NaVO3, and PI, respectively. The Bradford method was used to quantify the total protein concentration, after that 5Â protein sample buffer was properly added to the protein lysate then boiled at 95 C for 10 min. The PVDF membranes were blotted with mouse anti-BAX (1:1000), and anti-CA-IX (1;500), rabbit polyclonal anti-XIAP (1;1000), anti-Bcl2 (1:500), anti-STAT-3 (1:1000), and anti-pSTAT-3 (1:500). The PVDF membranes were transferred in rotating shaker at 4 C for immunoblotted against anti-mouse and anti-rabbit IgG (1:2000 dilution, Santa Cruz Bio Inc., Santa Cruz, CA). The immunoblot levels of proteins were observed using chemiluminescence system and relative band intensities were calculated using ImageJ software (Bethesda, MD).

Tumoricidal role of BSM-0004 in xenograft model of nude mice
The immunodeficient 5 weeks old female nude mice were procured from ORIENT BIO Inc. (Seongnam-si, South Korea). The nude mice were acclimatised in suitable condition and provided enough water and food routinely. The full experimental plan and methods were designed and approved by the Sookmyung Women's University's Board of Review Committee, and the Department of Animal Facility, Yongsan-gu, Seoul, Republic of Korea. The efficacy of BSM-0004 against MCF7 induced tumour burden in nude mice was carried out as described previously [51][52][53][54] . Briefly, totally 15 mice were sub-grouped in control (n ¼ 6), treatment (n ¼ 6), and normal (n ¼ 3) mice group, respectively. To generate tumour, 3 Â 10 6 cells were injected (by needle syringe, 27 G/12.7 mm, Ultra-Thin Plus TM , Seoul, South Korea) onto left and right flank of nude mice, then the treatment group mice were administered with 15 mg/kg BSM-0004 and control group mice were treated with DMSO. After 4th post days of inoculation, tumours were visible and started measuring the tumour volume followed with volume ¼ 1/2 Â length Â width 2 . Lastly, after finishing the experiment the mice were sacrificed followed by Sookmyung Women's University's standard protocol.

In vivo histopathological analysis of tumour samples
The efficacy of BSM-0004 and oncogenic expression of tumour marker Ki67 and hCA-IX were evaluated by immunohistochemistry of tumour samples as described in the previous study [51][52][53][54] . Briefly, tumour tissues were fixed with 4% paraformaldehyde then embedded with high grade of paraffin. The tissue-sections were cut in 5 mm thick using a microtome (Leica RM2255 Fully Automated Rotary Microtome, Wetzlar, Germany), then sliced tissues were fixed onto glass slides and deparaffinised with various percentage of xylene subjected to rehydrated with different concentrations of alcohol (C 2 H 5 OH). All slides were incubated with methanol containing 3% of hydrogen peroxide to be retrieved. After blocking with 5% horse serum, the slides were incubated with rabbit polyclonal antibodies for Ki67 (diluted, 1:400; Goat poly, Santa Cruz, Santa Cruz, CA) and anti-CA-IX (1:500 diluted, Mouse, abcam ab107257, Cambridge, UK). The immunohistopathological expression of Ki67 and hCA-IX was counter stained with biotinylated anti-rabbit and anti-mouse IgG (Vector Laboratories, Burlingame, CA). The diaminobenzidine (DAB) substrate was used to develop and precipitate the protein. Lastly, Mayer's haematoxylin blue staining was performed to distinctly observe nuclear portion. The Ki67 and hCA-IX protein levels were detected using Leica microscope system (Wetzlar, Germany).

Statistical analysis
All data were analysed using the GraphPad Prism5 software (GraphPad Prism Software, La Jolla, CA). Data were obtained and mean ± SD used for significant analysis. The Mann-Whitney test was performed to analyse the tumour weight in BSM-0004 treated vs. control group. On the other hand, rest of experiments were analysed using the one-way analysis of variance (ANOVA) test was performed to elucidate statistical differences and p< .05 was opted as a statistically significant.

BSM-0004 strongly inhibits hCA IX
In vitro CA inhibition study indicates that BSM-0004 effectively inhibits cancer-associated hCA IX in the nanomolar range. The result displayed that BSM-004 showed a Ki value of 95.1 nM against hCA IX. However, BSM-0004 displayed a Ki value of 8368.4, 962.2, and 750.9 nM against hCA I, hCA II, and hCA VII, respectively, that indicates selective inhibition towards hCA IX 13 . Hence, BSM-0004 emerged as a selective hCA IX inhibitor over other isoforms (Figure 1(A)).  MCF-7 cells as compared to MDA-MA-231 cells in normoxic as well as hypoxic conditions. Additionally, a phase contrast microscopy study also authenticated the cytotoxicity efficacy of BSM-0004 against MCF-7 cells where significant cell death was visualised with the treatment of BSM-0004. Moreover, treatment of BSM-0004 also inhibited colony formation of MCF-7 cells in a concentration-dependent manner that further confirmed the anti-proliferative action of BSM-0004 against MCF7 cells (Figure 1(D,E)).

BSM-0004 arrests G2/M phase of cell cycle and persuades apoptosis
A flow-cytometric analysis was conducted to examine the cell cycle arresting potential of BSM-0004 at 10 and 20 mM concentrations under normoxia and hypoxia conditions. The result evoked that BSM-0004 efficiently arrested the G2/M phase of the cell cycle and significantly increased the population of MCF7 cells, concentration-dependently as compared to control. In normoxia condition, BSM-0004 displayed 28.6 and 22.2% cell population in G2/M phase at 10 and 20 mM concentration, respectively, whereas untreated cells showed 18.3% cells in this phase (Figure 2(A)).
However, in hypoxia condition, 17.8 and 21.8% cell population were seen in the G2/M phase after treatment of BSM-004 at 10 and 20 mM concentrations, respectively, as compared to untreated cells that exhibited 17.6% cells (Figure 2(A)). Further, we also confirmed the apoptotic action of BSM-0004 in MCF7 cells by double staining method using PI and Annexin-V-FITC (Figure 3(A,B)). As displayed in Figure 3(A-C), BSM-0004 treatment at the concentration of 10 and 20 mM exerted 3.1 and 20.5% apoptosis, respectively, in MCF7 cells compared to control (showing 0.1%) under normoxic condition, while 12.7 and 14.8% apoptotic cells were observed at 10 and 20 mM concentrations in hypoxic condition as compared to control (showing 1.2%). In addition to this, the apoptotic effect of BSM-0004 was also confirmed by DAPI staining, where significant nuclear fragmentation was pictured upon treatment of BSM-0004 to MCF7 cells as compared to control (Figure 3(C)).

BSM-0004 impedes MCF-7 cells growth targeting CA IX
Our result indicated hypoxia significantly enhanced the expression of hCA IX in a time-dependent manner as compared to normoxic cells. Interestingly, treatment of BSM-0004 significantly reduced hypoxia-induced hCA IX expression in MCF7 cells time-dependently. However, a significant reduction in expression of hCA IX was observed in 72 h of BSM-0004 ( Figure 4). Thus, this study indicates that BSM-0004 induced apoptosis by targeting hCA IX as observed in hCA inhibition study in which BSM-0004 effectively inhibited hCA IX, displaying nanomolar range Ki value.

Effect of BSM-0004 on apoptotic biomarkers
To confirm the effect of BSM-001 treatment on various apoptotic biomarkers such as Bcl2, XIAP, p-STAT-3, and STAT-3, a western blot study was performed. Our investigation showed that treatment of BSM-004 significantly decreases the expression of Bcl2 under the hypoxic condition as compared to untreated cells. Additionally, another anti-apoptotic biomarker XIAP was also found to be downregulated after treatment of BSM-0004 as compared to control. Moreover, treatment of BSM-0004 also down regulated transcriptional factor STAT-3 as compared to untreated cells. Hence, these results evidently indicate that BSM-0004 effectively regulates apoptotic biomarkers associated with apoptosis, which leads to induce cell death in MCF-7 cells ( Figure 5(A-F)).

BSM-0004 obstructs tumour growth in vitro and in vivo
The anti-tumour effect of BSM-0004 has been evaluated in vitro using tumorsphere formation assay at 10 mM and 20 mM concentration under normoxic as well as hypoxic conditions. Result evidently suggested that BSM-0004 at 20 mM significantly halted tumorsphere formation of MCF7 cells as compared to untreated cells in both conditions normoxia and hypoxia ( Figure 6(A,B)). This experimental evidence clearly indicates the tumoricidal effect of BSM-0004 under hypoxic as well as normoxic conditions. Further, the in vivo anti-breast cancer activity of BSM-0004 has been examined at the dose of 15 mg/kg body weight in the breast cancer xenograft model using BALB/c-nu nude mouse. Upon development of tumour model, 15 mg/kg dose of BSM-0004 has been given for 26 days and control animal only received vehicle. The result directed that BSM-0004 significantly reduced tumour volume time-dependently as compared to the control group ( Ã p<.05) (Figure 6(E)). Additionally, BSM-004 treatment significantly ( ÃÃ p<.0012) decreased tumour weight as compared to untreated animals, indicating effective tumoricidal efficacy. It was noted that treatment of BSM-004 decreased almost 50% tumour weight as compared to untreated group's animals. Remarkably, it was also observed that BSM-0004 treatment for 26 days did not decline the body weight of the animals as compared to vehicle treated group that signifies a non-toxic nature of BSM-0004 ( Figure 5(D)). Moreover, BSM-0004 treated animals were examined carefully during the whole experimental period to visualise any significant symptoms associated with toxicity of drug. Notably, BSM-0004 treated animals did not demonstrate any kind of abnormal symptoms associated with toxicity such as alteration in behaviour, low food as well as water intake.

Histopathological examination of xenograft tumour
Furthermore, the ex vivo antitumor effect of BSM-0004 was also examined by performing an IHC study of breast cancer biomarker Ki67 and hCA IX. As results shown in Figure 6(H), a significant reduction in expression of Ki67 was observed in the tumour of BSM-0004 treated mouse as compared to the untreated mouse that further indicates the tumoricidal effect of this novel hCA IX inhibitor. A reduced expression of hCA IX has been also seen in the tumour of BSM-0004 treated animals compared to untreated animals indicating down regulation of hCA IX in xenograft mice model. Thus, these results endorse in vivo anticancer efficacy of BSM-0004 through hCA IX inhibition.

Discussion
Indeed existing treatment strategy for cancer is suffering from several complications such as severe side effects, ineffectiveness as well as drug resistance 55,56 . Therefore, there is an urgent need to explore new targets for the therapy of cancers that may pave the way to overcome drug resistance. In the past decade, hCA IX has been thoroughly studied and validated as an effective druggable target for the therapy of cancers 57,58 . To impede cancer growth by targeting hCA IX, several inhibitors of this enzyme have been developed in the past years. However, most of them are being studied in the pre-clinical as well as the clinical trial stages 19 . Therefore, the development of new hCA IX inhibitors with promising anticancer activity is still desired.
In this line, we have evaluated in vitro as well as in vivo anticancer action of novel hCA IX inhibitor BSM-0004. Anticancer effectiveness along with its mechanism of action has been Uncontrolled cell division promotes the growth and proliferation of cancerous cells. Targeting cell cycle checkpoints is considered an effective tool to control the proliferation of cancer cells and several anticancer agents target various checkpoints of the cell cycle to avert cell division in cancer cells 59 . Thus, the effect of BSM-0004 on cell cycle regulation was analysed under normoxic as well as hypoxic conditions. Noticeably, BSM-004 significantly enhanced MCF 7 cell population in the G2/M phase as compared to untreated cells and thus, successfully arrested the G2/M phase of the cell cycle.
Induction of apoptosis in cancerous cells is an imperious strategy to stop their uncontrolled proliferation and many anticancer drugs follow this pathway to kill cancer cells 60 . Captivatingly, BSM-004 (10 mM and 20 mM) actively induces apoptosis in MCF 7 under normoxic as well as hypoxic conditions as analysed by FACS analysis. Further, DAPI staining also confirmed apoptotic action of BSM-004 in MCF 7 cells in which significant morphological alterations such as chromatin condensation and nuclear fragmentation were visualised upon treatment of this compound in both conditions normoxia as well as hypoxia.
After this, we inspected the effect of BSM-004 in the regulation of anti-apoptotic and pro-apoptotic biomarker proteins that play an important role in cell fate. Anti-apoptotic protein Bcl2 opposes cell death by obstructing the movement of cytochrome c from mitochondria, though pro-apoptotic protein Bax induces apoptosis 61 . It was noticed that BSM-0004 significantly reduces the expression of Bcl2 and augments the expression of Bax in MCF 7 cells under hypoxia conditions. X-linked inhibitor of apoptosis protein XIAP is known as an inhibitor of apoptosis and its downregulation encourages cell death 62 . Our western blot analysis revealed that BSM-0004 treatment to MCF 7 cell significantly diminishes expression of apoptotic inhibitor XIAP under hypoxia condition. Furthermore, we also investigated the regulation of STAT-3 after treatment of BSM-0004 to MCF 7 cells under hypoxia condition. Evidence directs that STAT-3 significantly contributes to tumourigenesis by up-regulating the expression of several anti-apoptotic proteins. Noticeably, it was found that treatment of BSM-0004 significantly lowered the expression of STAT-3 that further authenticated its anti-proliferative action.
We extended our study to investigate in vivo anticancer effect of BSM-0004 against MCF 7-xenograft nude mice model. Palpably, BSM-0004 at the dose of 15 mg/kg/bwt displayed effective anticancer activity against this model and significantly reduced tumour weight and tumour volume as compared to untreated animals. Interestingly, no deaths were recorded in BSM-0004 treated animals during the whole experimental period. Additionally, All BSM-0004 treated animals were found normal and no sign of severe toxicity associated with drug treatment was observed.
Finally, an IHC study of animal's tumour has been performed to examine the ex vivo anticancer effect of BSM-0004. Indeed IHC experiment indicated that BSM-0004 significantly reduced expression of breast cancer biomarker Ki67 as compared to the untreated animals. Markedly, a low expression of hCA IX was also observed in the tumours of BSM-0004 treated animals as compared to the tumours of the untreated group.
Thus, this finding further verifies that the anticancer effect of BSM-0004 is governed by hCA IX inhibitory action. Overall, these in vivo experiments visibly indicate that BSM-0004 has promising in vivo anticancer activity without any severe side effects.

Conclusions
Herein, we disclose in vitro and in vivo anti-breast cancer activity of novel hCA IX inhibitor BSM-0004. BSM-0004 showed effective cytotoxic effect against MCF 7 cells under normoxic as well as hypoxic conditions, being able to impede colony formation of MCF7 cells. This novel hCA IX inhibitor effectively targeted G2/M phase of cell cycle and induced apoptosis in MCF 7 cells under normoxic as well as hypoxic conditions. In addition, this compound promisingly regulated expression of apoptosis associated biomarkers that further authenticates its in vitro anticancer effectiveness. Moreover, BSM-0004 halted formation of tumour spheroids of MCF 7 cells under normoxic as well as hypoxic conditions in concentration dependent manner. Noticeably, BSM-0004 significantly inhibited tumour progression in MCF-7 xenograft nude mice model, lowering tumour weight, and tumour volume in BSM-0004 treated mice as compared to untreated mice. Interestingly, reduced expression of cancer biomarkers Ki 67 and hCA IX was noticed in the tumours of BSM-0004 treated animals as compared to untreated animals that further advocate anti-cancer supremacy of this novel compound through hCA IX inhibition. Hence, this investigation offered a promising anti-breast cancer agent which showed effective anti-cancer action in vitro and in vivo, targeting hCA IX. Disclosure statement