Tuning the activity of iminosugars: novel N-alkylated deoxynojirimycin derivatives as strong BuChE inhibitors

Abstract We have designed unprecedented cholinesterase inhibitors based on 1-deoxynojirimycin as potential anti-Alzheimer’s agents. Compounds are comprised of three key structural motifs: the iminosugar, for interaction with cholinesterase catalytic anionic site (CAS); a hydrocarbon tether with variable lengths, and a fragment derived from 2-phenylethanol for promoting interactions with peripheral anionic site (PAS). Title compounds exhibited good selectivity towards BuChE, strongly depending on the substitution pattern and the length of the tether. The lead compounds were found to be strong mixed inhibitors of BuChE (IC50 = 1.8 and 1.9 µM). The presumptive binding mode of the lead compound was analysed using molecular docking simulations, revealing H-bond interactions with the catalytic subsite (His438) and CAS (Trp82 and Glu197) and van der Waals interactions with PAS (Thr284, Pro285, Asn289). They also lacked significant antiproliferative activity against tumour and non-tumour cells at 100 µM, making them promising new agents for tackling Alzheimer’s disease through the cholinergic approach.


Introduction
Imino-and azasugars, that is, carbohydrate mimetics where the endocyclic oxygen or a carbon atom, respectively, has been replaced by a nitrogen atom that has attracted great attention within Medicinal Chemistry since several decades ago 1 . The key structural motif of such glycomimetics is a protonable nitrogen atom, which might allow them to mimic transition states (in terms of charge and geometry) 2 of the glycosidase-mediated hydrolysis of glycosidic bonds. Because of this, many imino-and azasugars behave as potent inhibitors of ubiquitous glycosidases and glycosyltransferases 1 , key enzymes in a plethora of biological processes where carbohydrates are involved, including metabolic pathways, cell wall formation, and recognition events. Numerous imino-and azasugars have shown relevant pharmacological activities 3 , such as antidiabetic 4 , anticancer 5 , antifungal 6 , antiprotozoal 7 or antiviral agents 8 , among others. In this sense, Sirona Biochem Corp. has just announced 9 the launching of an intense programme searching for new antivirals for combating the pandemic caused by COVID-19, in which iminosugars are one of the three categories in the study. This choice is strongly supported by the World Laureates Association Shanghai Centre. Some iminosugars have also been found to act as chaperones 10 against lysosomal storage disorders, like Gaucher's 11 and Fabry's 12 diseases. Iminosugars have also been found to be effective against cystic fibrosis 13 , an autosomal recessive disease-causing severe multi-system organ damage, particularly to the respiratory system. Iminosugars can act as correctors of defective cystic fibrosis transmembrane conductance regulator (CFTR) 14,15 , an ABC transporter-class protein that controls the content of ion and water in epithelial cells, or alternatively they can reduce the inflammatory response of pathogens, like P. aeruginosa 16 . More recently, conjugation of iminosugars with sulphonamides resulted in the inhibition of relevant human carbonic anhydrases 17 . Among the vast number of iminosugars and related structures reported so far, 1-deoxynojirimycin (1-DNJ), a natural iminosugar isolated from mulberry leaves 18 , is the basis for the so-far two marketed drugs derived from iminosugars: Miglitol V R (N-hydroxyethyl-1-deoxynojirimycin) 19 and Zavesca V R (N-butyl-1-deoxynojirimycin) 20 , used for tackling non-insulindependent diabetes, and Gaucher's disease, respectively.
Nevertheless, a field that still remains relatively unexplored concerning iminosugars is Alzheimer's disease. Alzheimer's is a devastating neurodegenerative disease, considered nowadays the most prevalent form of dementia, counting for roughly 70% of total cases 21 . It causes severe impairment of cognitive functionality, neurodegeneration, and even Parkinsonian symptoms, leading the patient to a complete dependence even for accomplishing daily tasks 22 . According to the World Alzheimer Report 2019, roughly 50 million people have been diagnosed worldwide, and this number is expected to increase almost exponentially to 152 million people by 2050 23 . Another worrying aspect is that, although Alzheimer's disease has been classically associated with elderly people, the average age of patients has decreased significantly 24 .
From a pathophysiological point of view, Alzheimer's disease is one of the most complex diseases nowadays, whose multifactorial aetiology is not even completely understood. This hampers enormously finding a treatment. The most recognised hallmarks are 25 the deposit of toxic amyloid plaques, neurofibrillary tangles (via hyperphosphorylation of tau proteins), and low levels of the neurotransmitter acetylcholine, which is associated with the cognitive decline.
Currently, there are four marketed drugs for ameliorating the symptoms of Alzheimer's disease 26 aiming at two therapeutic targets: the inhibitors of cholinesterases (acetylcholinesterase, AChE; and butyrylcholinesterase, BuChE) donepezil, rivastigmine and galantamine, which increase the levels of acetylcholine (cholinergic hypothesis), and the antagonist of N-methyl-D-aspartic receptors (NMDA) memantine, for regulating glutamate levels in brain cells.
In connection with iminosugars, Zavesca V R was recently found to reduce the amyloid plaque production in a cell model 27 . Compain and co-workers analysed the cholinesterase inhibition of a series of iminosugars with different substituents motifs and stereochemistry 28 . In our research group, we recently combined 1-DNJ with a selenoureido appendage to achieve strong b-glucosidase and AChE inhibitors 29 . To the best of our knowledge, these are the only examples of iminosugars exhibiting anti-Alzheimer's properties.
Our main target herein has been the design of new potential AChE and/or BuChE inhibitors against Alzheimer's disease using 1-DNJ as the core template. Rationalisation of their biological properties by analysing interactions with key enzymatic domains is also covered herein.

Materials and methods
General procedures 1 H (300.1 MHz) and 13 C (75.5 MHz) NMR spectra were recorded at 25 C on a Bruker Avance 300 spectrometer using the deuterated solvent indicated in each case. 1 H and 13 C assignments were confirmed by 2D COSY and HSQC experiments. Mass spectra (ESI) were recorded on a Q Exactive mass spectrometer. TLCs were performed on aluminium pre-coated sheets (E. Merck Silica gel 60 F 254 ); spots were visualised by UV light, and by charring with 10% vanillin in EtOH containing 1% of H 2 SO 4 , or with 10% H 2 SO 4 in EtOH. Column chromatography was performed using E. Merck Silica Gel 60 (40-63 lm), using the eluent indicated in each case.

Chemistry
General procedure for the preparation of compounds 7 and 8 To a solution of the corresponding 2-arylethanol derivatives 14a-g (2.19 mmol) in DMF (5 ml), 60% NaH (525 mg, 13.14 mmol, 6.0 equiv.) was added and the resulting mixture was kept stirring at rt under an inert atmosphere for 30 min. Then, 1,6-dibromohexane or 1,5-dibromopentane was added (17.52 mmol, 8.0 equiv.), and stirring was kept for a further 5 h in the case of 7, and 4 h in the case of 8. The reaction was quenched by the careful addition of water at 0 C. After that, the crude reaction mixture was diluted with brine (50 ml) and extracted with ethyl acetate (3 Â 50 ml). The combined organic fractions were dried over Na 2 SO 4 , filtered and the filtrate was concentrated to dryness. The residue was purified by column chromatography, using the eluent indicated in each case to give compounds 7 and 8 as colourless oils (See Supplementary Material).
General procedure for the preparation of compounds 9 and 10 To a solution of per-O-benzylated 1-deoxynojirimycin 6 (250 mg, 0.48 mmol) in DMF (5 ml), K 2 CO 3 (199 mg, 1.44 mmol, 3.0 equiv.) and O-alkylated derivatives 7 or 8 (1.19 mmol, 2.5 equiv.) were added. The corresponding mixture was kept stirring at 85 C for 15 h. Then water was added, and products were portioned between brine (50 ml) and EtOAc (3 Â 50 ml). The combined organic fractions were dried over Na 2 SO 4 , filtered and the filtrate was concentrated to dryness under reduced pressure. The residue was purified by column chromatography using the eluents indicated in each case, to give compounds 9 and 10 as colourless syrups (See Supplementary Material).
General procedure for the preparation of compounds 11 and 12 To a solution of benzylated derivative 9 or 10 (0.20 mmol) in a 1:1 CH 2 Cl 2 -MeOH mixture (6 ml), AcOH (0.4 ml) and Pd(OH) 2 /C (150 mg) were added, and the corresponding mixture was hydrogenated for 24 h. After that, it was filtered over a Celite V R pad and washed with MeOH. The filtrate was concentrated to dryness and purified by column chromatography (190:10:1 ! 140:60:1 CH 2 Cl 2 -MeOH-Et 3 N) in the case of 11. For compounds 12, no further purification was accomplished after filtration. Derivatives 11 and 12 were obtained as colourless syrups (See Supplementary Material).

Enzymatic assays
For both families of enzymes used herein (glycosidases and cholinesterases), stock solutions of the inhibitors were prepared in DMSO; DMSO content was kept at 5% (V/V) for glycosidases and 1.25% (V/V) for cholinesterases. Enzymes were properly dissolved in water in a concentration in which the rate of the reaction when using [S] ¼ 4 Â K M is in the range 0.12-0.15 Abs/min. In both kinds of enzymes, an initial screening at 100 mM inhibitor concentration was performed, using the following substrate concentrations: [S] ¼ 0.25 mM for a-glucosidase (Saccharomyces cerevisiae), 4.0 mM for b-glucosidase (almonds), 0.60 mM for a-galactosidase (green coffee beans), 0.51 mM for b-galactosidase (Escherichia coli), 1.5 mM for b-galactosidase (Aspergillus Oryzae), 0.12 mM for AChE (Electrophorus electricus), 0.11 mM for BuChE (equine serum). When the percentage of inhibition was higher than 50%, the kinetic parameters and the inhibition constants were calculated using five different substrate concentrations ranging from 1 =4 K M to 4 Â K M , and 2-4 different inhibitor concentrations (affording roughly 20-70% inhibition). Each assay was run in duplicate.
Glycosidase assays were accomplished using the methodology reported by Bols and co-workers 30 . Each set of experiments is prepared in PS cuvettes using 0.1 M phosphate buffer (pH 6.8) and the corresponding oor p-nitrophenyl glycopyranosides as substrates. Reactions were monitored at 25 C by following the formation of the corresponding nitrophenolates at 400 nm (glucosidases and a-galactosidase) or 420 nm (b-galactosidases) over a time of 125 s.
For cholinesterases, minor modifications on Ellman's assay 31 were performed. The activity was measured in PS cuvettes containing 0.1 mM phosphate buffer (pH 8.0), 0.88 mM DTNB, and substrate (acetyl-and butyryl-thiocholine iodides for AChE and BuChE, respectively), The formation of the chromophore was monitored at 405 nm over a time of 125 s.
For strong inhibitors, the mode of inhibition was determined using the Cornish-Bowden method, which involves the use of two different plots: 1/v vs.
[I] (Dixon plot) and [S]/v vs. [I]. For the calculation of kinetic parameters (K M , V max ) a nonlinear regression analysis (least squares fit, GraphPad Prism 8.01) was used. Data are expressed as the mean ± SD.

Docking simulations
Interactions of cholinesterases with the compounds were analysed by computational docking using MOE software (Chemical Computing Group). Crystallographic structures of human AChE and human BuChE was obtained from Protein Data Bank (PDB code 4EY6 and 4AQD, respectively). Protein structures was energetically minimised using Amber10 force field with EHT parameters for small molecules, R-field solvation model, dielectric constant of 1 for the protein interior and 80 for the exterior. Ligand structure was drawn in MOE software, and its energy was minimised with the above parameters using as stop criterion an RMS gradient lower than 0.01 kcal/mol/Å. For the docking calculations: in the placement stage we used the Triangle Matcher algorithm with the London dG scoring scheme. In the refinement stage, we kept the receptor rigid and used the GBVI/WSA dG scoring scheme. 2D diagrams were obtained from MOE software and 3D illustrations were obtained using Pymol software.

Antiproliferative assays
We selected the human neuroblastoma cell line SH-SY5Y and the fibroblast cell line BJ-hTERT to evaluate the antiproliferative activity of the compounds. The tests were performed in 96-well plates using the SRB assay 32 with the following specifications. Cell seeding densities were 5000 cells/well for SH-SY5Y and 7000 cells/well for BJ-hTERT. Drug incubation times were 48 h. The optical density of each well was measured at 530 (primary) and 620 (secondary) nm. The antiproliferative activity expressed as 50% growth inhibition (GI 50 ), was calculated according to NCI formulas 33 .

Chemistry
In terms of inhibition, AChE contains two key regions 34 : the catalytic anionic site (CAS), which enables cation-p interactions with the quaternary ammonium fragment of acetylcholine, the natural substrate of the enzyme, and the peripheral anionic site (PAS), enriched with aromatic amino acid residues, and located roughly 15 Å from CAS through a narrow gorge, capable of establishing interactions with planar and aromatic residues. It is important to mention that PAS is also involved in the initial step of b-amyloid aggregation 35 . Accordingly, dual inhibitors establishing favourable interactions with both, CAS and PAS, can be not only strong inhibitors, but they can also contribute to ameliorate the formation of neurotoxic amyloid plaques.
Inspired by the structure of AChE, we propose herein the preparation of a series of iminosugars-based cholinesterase inhibitors. The general structure for such derivatives is depicted in Figure 1, with three key structural motifs: the iminosugar residue, an alkoxy tether, and an aromatic motif with different substitution patterns. Oxygenated N-alkyl iminosugars have been found to reduce cytotoxicity when evaluated as antiviral agents 36 , thus diminishing side-effects. In particular, we envisioned the possibility of using 1-DNJ as the key core.
On the one hand, N-alkyl-DNJ derivatives could be partially protonated at physiological pH, thus mimicking the ammonium cation of acetylcholine, and enabling favourable interactions within the CAS. Although it has been reported that the presence of aromatic substituents connected to iminosugars through a short alkyl tether (2-3 carbon atoms) leads to pKa of 6.0 37 or below 6.0 38 , because of the electron-withdrawing effect of the aryl moiety, compounds prepared herein, with longer alkyl fragments are expected to tentatively exhibit higher pKa values, probably in between for those reported for N-nonyl-DNJ and N-butyl-DNJ (6.7 and 7.1, respectively) 39 . On the other hand, as aforementioned, 1-DNJ has positive effects against amyloidogenesis. Moreover, 1-DNJ has recently been found 40 to ameliorate stable angina pectoris in patients with coronary heart disease by reducing inflammatory responses and by increasing the antioxidant machinery. Taking into consideration that profound oxidative stress and neuroinflammation are common features of Alzheimer's disease 41 , the use of 1-DNJ as the pharmacophore might also provide activity against other targets of this multifactorial disease. Furthermore, different substitution patterns on the aromatic appendage might modulate the interactions within the PAS region.
For accessing compounds 11 and 12, depicted in Scheme 1, the key intermediate is per-O-benzylated 1-DNJ 6, which was accessed in a 5-step linear pathway (36% overall yield), following the procedure reported in the literature 42 , starting from commercially-available methyl a-D-glucopyranoside 1 (Scheme 1). Such procedure involves fully O-protection, acidic hydrolysis of the glycoside moiety, reduction of the masked aldehyde of 3, Swern oxidation 43 , and fast reductive amination of transient unstable dicarbonyl compound 5; the last step consists of a double reductive amination (DRA), a cascade reaction that provides a straightforward methodology for accessing polyhydroxylated piperidines 44 . Base-promoted N-alkylation with bromoderivatives 7 and 8, and deprotection by hydrogenolysis, furnished compounds 11 and 12, respectively, with the substitution pattern depicted in Scheme 1. We hypothesised that the presence of one or two methoxy groups could resemble the dimethoxy arene-based fragment of the indanone moiety in donepezil, and thus, might enable interaction with the PAS region of cholinesterases. Moreover, the use of a o-dihydroxyphenyl ring (catechol moiety) could afford relevant antioxidant properties 45 , as well as the capacity for chelating metal ions (Zn, Cu, Fe are present at toxic levels in the brain of Alzheimer's patients) 46 .
To avoid side-reactions, those starting materials bearing free phenolic hydroxyl groups (natural tyrosol 13b, hydroxytyrosol 13d and homovanillic alcohol 13f) were protected using a chemoselective benzylation under mild basic conditions (See Supporting  Material, compounds 14b, d, and f). Moreover, for the scaffold incorporating a methylidene motif, commercially-available 2-  47 . It has been reported 48 that such conditions lead to the in situ generations of borane, which reduces efficiently the carboxyl group to a primary alcohol. Such treatment furnished 14g in a quantitative yield after chromatographic purification.
Mono-alkylation of 2-arylethanol derivatives 14a-g was accomplished using an excess of 1,6-dibromohexane under basic conditions (NaH) in general from excellent to quantitative yields (Scheme 1) to give 7, 8. Alkylation on the endocyclic nitrogen atom of 6 (Scheme 1) was more complicated than previously anticipated. Nucleophilic displacement of 6 on the terminal bromine atom of 7a-g accomplished derivatives 9a-g in moderate yields (46-68%). Final deprotection using standard hydrogenolysis conditions furnished derivatives 11a-g in moderate to quantitative yields.
To analyse the influence of the linkage length on the biological properties, homologous 12a, c, and d were also prepared as representative examples (Scheme 1). Noteworthy, extraordinary differences in activity towards cholinesterases were found by our group upon assessment of tacrine-derived homo-and heterodimers as anti-Alzheimer's agents by small variations in the tether length 49 . Again, the limiting step in terms of yields was the N-alkylation of O-protected 1-DNJ 6. Analogously, previous attempts 50 to alkylate the same position on azafagomine, a cyclic hydrazine analogue of 1-DNJ via nucleophilic substitutions failed. This was attributed to the high sterical hindrance exerted by the bulky exocyclic benzyloxymethyl moiety, in its major gt conformation, that occluded the entry of the reagents.

Biological assays
The ten 1-DNJ derivatives prepared herein (11a-g, 12a, c, and d) were subjected to inhibition assays on two families of enzymes: glycosidases and cholinesterases. In both cases, an initial screening was performed at 100 mM inhibitor concentration. For those compounds exhibiting a percentage of inhibition higher than 50% under such conditions, the kinetic parameters (K M,app and V max,app ) were obtained, that were used for calculating the inhibition constants (K i 's). The mode of inhibition was also established for the most potent compounds, using the Cornish-Bowden method 51 .

Glycosidase and cholinesterase inhibition studies
As aforementioned, glycosidases play a pivotal role in controlling not only the metabolic pathways where carbohydrates are present but also many other significant biochemical events, like cell-to-cell communication or recognition processes. Herein, a panel of five commercially-available glycosidases have been tested, as models of carbohydrate-mediated diseases: a-glucosidase (from Saccharomyces cerevisiae), b-glucosidase (from almonds), a-galactosidase (from green coffee beans) and b-galactosidase (from A. oryzae and from E. coli). Deficiencies in such enzymes are associated with diabetes, Gaucher's disease, Fabry's disease, and gangliosidosis, respectively 3 .
Assessment of the inhibitory capacity against glycosidases was accomplished using the methodology reported by Bols and coworkers 30 . p-Nitrophenyl glycosides (o-for b-galactosidases) were used as model substrates, and the release of the corresponding pnitrophenolates at pH 6.8 was monitored spectrophotometrically (k max ¼ 400 or 420 nm for por o-nitrophenolates, respectively).
Selected results are shown in Table 1, where the IC 50 and inhibition constants values, together with the mode of inhibition are depicted. 1-DNJ is a good inhibitor of a-galactosidase and moderate of a-glucosidase (IC 50 ¼ 16 and 35 mM, respectively), and a weak inhibitor of b-glucosidase (IC 50 ¼ 71 mM). However, compounds 11 and 12 lacked significant activity against a-glucosidase and a/b-galactosidases (Table 1 and Table S1), and regarding b-glucosidase, compounds 11 ranged from good (11b, d, and e, IC 50 : 12-15 mM) to strong competitive inhibitors (11a, c, f, and g, IC 50 : 4.6-8.4 mM). This means a complete reversal of selectivity towards b-glucosidase, with a 15-fold increased activity compared to natural 1-DNJ, suggesting that the more lipophilic substituents favour interaction with b-glucosidase. The same behaviour was found upon alkylation on N-2 position of azafagomine 50 . Interestingly, a decrease in the tether length (compounds 12) was found to be detrimental for the inhibition of b-glucosidase (e.g. 4-6-fold for 12a, c), showing the great influence of such structural motif.
Derivatives 11 and 12 were tested also against AChE (Electrophorus electricus) and BuChE (equine serum), as recognised models for human cholinesterases due to their close structural resemblance 52 . For that purpose, Ellman's colorimetric assay was used; this is an indirect test in which acetyl and butyryl thiocholine iodides are used as model substrates, which upon interaction with the enzyme release thiocholine, which in turn reacts with the chromogen reagent 5,5 0 -dithiobis(2-nitrobenzoic acid (DTNB) to give 2-nitro-5-mercaptobenzoate (at pH 8.0). The monitoring of the latter at 405 nm furnishes the kinetic parameters.
Interesting structure-activity relationships can be extracted also from activity data (Table 1). Regarding AChE, clearly, the presence of a dimethoxyphenyl moiety furnishes the most potent compound (11e, IC 50, and Ki's in the low micromolar range, mixed inhibitor), as was initially hypothesised.
However, what is more, interesting about the anticholinergic activity of these compounds is that they show a clear preference towards BuChE. Thus, relatively lipophilic disubstitution (11e-g) affords the strongest compounds in the series, within the low micromolar range (IC 50 1.8-7.3 mM) (di-OMe%OMe/OH > OCH 2 O).
An issue that must be addressed is that BuChE has a more prominent role than AChE in elderly people and in more advanced stages of Alzheimer's disease 53 . Moreover, BuChE has also been reported to participate in amyloid plaques and neurofibrillary tangles-mediated neurodegeneration 54 . This means that compounds 11e-g could be useful not only for re-establishing the cognitive functionality of the patients (by increasing the levels of cholinesterase) but also for retarding or ameliorating neurotoxicity associated with amyloidogenesis in advanced stages of the disease.
The absence of substitution on the aromatic scaffold, monosubstitution, or the presence of a polar catechol moiety completely abolished activity.
Compounds 11e-g turned out to be either mixed or non-competitive inhibitors, as evidenced by the Cornish-Bowden plots. This means that the title compound can bind either the free enzyme (K ia ) or the complex enzyme-substrate (K ib ). In order to illustrate this issue, Cornish-Bowden plots for derivative 11e against BuChE (mixed inhibition) are depicted in Figure 2.
Docking studies Docking calculations predict that compound 11e forms a complex with AChE ( Figure 3). The iminosugar moiety establishes H-bond interactions with Trp86 and Glu202 at the cationic anionic subsite (CAS). Additionally, the aromatic ring moiety is involved in p stacking interactions with Trp286 at the peripheral anionic subsite (PAS). The aromatic ring also participates in van der Waals interactions with several residues at the peripheral anionic subsite.
Docking calculations also predict that compound 11e forms a complex with BuChE ( Figure 4). The iminosugar moiety establishes H-bond interactions with Trp82 and Glu197 at the cationic active subsite (CAS) and with His438 at the catalytic subsite. Additionally, the aromatic ring moiety is involved in van der Waals interactions with residues Thr284, Pro285, Asn289 at the peripheral anionic subsite (PAS). Taken together, these calculations predict that 11e interacts in similar modes with both enzymes (as seen in the 3D figures) except for the interaction with His438 in BuChE which is missing in AChE. The p stacking interactions with Trp286 seen in AChE are not present in BuChE because there is no equivalent residue at the BuChE peripheral anionic subsite.

Antiproliferative activity
To analyse the potential cytotoxicity of title compounds, their antiproliferative activity was measured. For that purpose, one solid tumour cell line (SH-SY5Y, glioblastoma) as a model of brain cells, and one non-tumour cell line (BJ-hTERT human fibroblasts) were used. Data show no disruption of cell growth for most of the compounds tested. Only catechol-containing 11d, exhibited a moderate antiproliferative effect against the non-tumour cell line (GI 50 ¼ 39 ± 5 mM). Interestingly, the rest of the compounds, and particularly the lead compounds (11e-g) lacked significant growth inhibition effects when tested as a concentration as high as 100 mM (GI 50 > 100 mM).

Conclusions
We have accomplished the straightforward preparation of hitherto unknown cholinesterases inhibitors based on the use of the iminosugar 1-DNJ as the key pharmacophore for struggling with the low levels of the neurotransmitter acetylcholine in Alzheimer's disease. Appendage of an ether-connected aromatic motif with different substitution patterns allowed the modulation of the activity towards cholinesterases. Compounds bearing relatively lipophilic disubstituted phenyl moieties (3,4-diOMe, 3-OH/4-OMe, 3,4-OCH 2 O) turned out to be strong inhibitors of AChE, and particularly of BuChE, with IC 50 and K i values within the low micromolar range. The presumptive binding mode of the lead compound to cholinesterases was analysed using molecular docking simulations, revealing interactions with two key regions of the enzyme, CAS, and PAS. Moreover, as well as the strong anticholinergic activity, the lack of remarkable growth inhibition effects at a concentration as high as 100 mM suggests a good profile for the development of future anti-Alzheimer's drugs based on iminosugars.