Synthesis and in vitro anti-proliferative activity of some novel isatins conjugated with quinazoline/phthalazine hydrazines against triple-negative breast cancer MDA-MB-231 cells as apoptosis-inducing agents

Abstract Treatment of patients with triple-negative breast cancer (TNBC) is challenging due to the absence of well- defined molecular targets and the heterogeneity of such disease. In our endeavor to develop potent isatin-based anti-proliferative agents, we utilized the hybrid-pharmacophore approach to synthesize three series of novel isatin-based hybrids 5a–h, 10a–h and 13a–c, with the prime goal of developing potent anti-proliferative agents toward TNBC MDA-MB-231 cell line. In particular, compounds 5e and 10g were the most active hybrids against MDA-MB-231 cells (IC50 = 12.35 ± 0.12 and 12.00 ± 0.13 μM), with 2.37- and 2.44-fold increased activity than 5-fluorouracil (5-FU) (IC50 = 29.38 ± 1.24 μM). Compounds 5e and 10g induced the intrinsic apoptotic mitochondrial pathway in MDA-MB-231; evidenced by the reduced expression of the anti-apoptotic protein Bcl-2, the enhanced expression of the pro-apoptotic protein Bax and the up-regulated active caspase-9 and caspase-3 levels. Furthermore, 10g showed significant increase in the percent of annexin V-FITC positive apoptotic cells from 3.88 to 31.21% (8.4 folds compared to control).


Introduction
Heading the list of the critical health problems for females, breast cancer continues to be the major life-threatening health issue. According to the recent records, an estimated 1.7 million women are expected to be diagnosed with breast cancer by 2020 with a make-up of 26% increase from current recorded levels 1,2 . Despite the huge and continuous efforts of the scientific community, no tangible improvements were recorded in the last decade. With the development of resistance toward the available anticancer drugs, the mission becomes even more challenging and the efficacious therapy remains questionable 3,4 .
Targeting breast cancer with tailored drugs depends on the fact of immunohistochemical expression of estrogen receptors (ERs), progesterone receptors (PRs) and human epidermal growth factor receptors-2 (HER-2). Being devoid of the three aforementioned targetable proteins, triple-negative breast cancer (TNBC) stands even as an unbeatable challenge 5 . Besides their well-known resistance to the current chemotherapy, TNBC is an aggressive phenotype of cancer with high recurrence risk, poor prognosis, reduced survival and distant metastasis that it may reach the lungs and even the central nervous system (CNS) 5,6 . Accordingly, patients largely receive systemic chemotherapy that puts them under higher risk of suffering the devastating side effects. This urgent necessity motivates medicinal chemists to search for alternative treatments targeting apoptotic machinery as a novel approach for TNBC therapy.
Targeting critical regulators of apoptosis with the goal of inducing apoptosis in cancer cells still stands as an attractive and successful strategy to drug discovery and development of new anti-cancer agents [7][8][9][10][11] . On April 11, 2016, the U.S. food and drug administration (FDA) approved Venetoclax (Venclexta V R ) for the treatment of patients with chronic lymphocytic leukemia (CLL) whose tumors have a specific genetic alteration. Venclexta is the first FDA-approved drug that targets the BcL-2 protein, an antiapoptotic regulatory protein 12 .
Molecular hybridization is a contemporary concept in drug design and development gaining momentum worldwide. There are two main ways that can be explored to design and construct affordable and efficient hybrid molecules. The first method merges haptophoric moieties of different drugs and the second technique combines two or more entire drugs together using a linker chain 13,14 . This strategy affords novel molecule hybrids with MB-231 cell line. Figure 2 displays some of these derivatives with their IC 50 values or growth inhibition (GI) percentage against MDA-MB-231 cells.
In light of the above findings and as a part of our ongoing effort to develop potent isatin-based anti-proliferative agents [39][40][41][42][43][44][45] , we utilized the hybrid pharmacophore approach to design and synthesize three different series of novel isatin-based hybrids 5a-h, 10a-h and 13a-c (Figure 2), via merging the pharmacophoric elements of isatin and phthalazine or quinazoline in a single chemical framework through a hydrazine linker, with the prime goal of developing potent anti-proliferative agents against the TNBC MDA-MB-231 cell line. The three synthesized series were in vitro evaluated for their potential anti-proliferative activity against TNBC MDA-MB-231 cell line. Compounds 5e and 10g were further estimated for their apoptosis induction potential in MDA-MB-231 cells, to gain mechanistic insights into the anti-proliferative activity of the prepared hybrids. Furthermore, the activities against A549 alveolar carcinoma, Caco-2 colon cancer, LoVo human colorectal carcinoma and HepG2 hepatocellular carcinoma were in vitro examined.

Chemistry
Melting points were measured with a Stuart melting point apparatus (Bibby Scientific Limited, Staffordshire, UK) and were uncorrected. Infrared spectra were recorded as potassium bromide discs on Schimadzu FT-IR 8400S spectrophotometer (Shimadzu Scientific Instruments, Kyoto, Japan) and expressed in wave number (cm À1 ). The NMR spectra were recorded on Varian Gemini-300BB 300 MHz FT-NMR spectrometers (Varian Inc., Palo Alto, CA). 1 H and 13 C spectra were run at 300 and 75 MHz, respectively, in deuterated dimethyl sulfoxide (DMSO-d 6 ). Chemical shifts (d H ) are reported relative to tetramethylsilane (TMS) as internal standard. All coupling constant (J) values are given in hertz. Chemical shifts (d C ) are reported relative to DMSO-d 6 as internal standards. The abbreviations used are as follows: s, singlet; d, doublet; m, multiplet. Elemental analyses were carried out at the Regional Center for Microbiology and Biotechnology, Al-Azhar University, Cairo, Egypt. Reaction courses and product mixtures were routinely monitored by thin layer chromatography (TLC) on silica gel precoated F 254 Merck plates (Merck Group, Darmstadt, Germany). Unless otherwise noted, all solvents and reagents were commercially available and used without further purification.
General procedure for preparation of 4-chloroquinazolines 2a,b To a cooled stirred mixture of phosphorus oxychloride (30 mL) and N,N-dimethylaniline (1 mL), 2-Substituted-quinazolin-4(3H)-ones 1a,b (10 mmol) were added portion-wise. After refluxing for 6 h, the reaction mixture was cooled then poured onto ice-water and  alkalinized with 2 N NaOH. The aqueous solution was extracted with methylene chloride. The combined organic layer was dried over anhydrous Na 2 SO 4 then filtered, and the solvent was removed under reduced pressure.
General procedure for preparation of 1-hydrazinyl-4-phenylphthalazines 9a,b Compounds 9a,b were prepared according to the literature procedure 49 .
General procedure for preparation of the target isatinphthalazine hybrids (10a-h) Hydrazino phthalazines 9a,b (1 mmol) and catalytic amount of glacial acetic acid were added to a stirred solution of indoline-2,3-dione 4a-d (1 mmol) in refluxing absolute ethyl alcohol, then the reaction mixture was refluxed for 1 h. The precipitated solid was filtered while hot, dried and recrystallized from ethanol/DMF mixture to afford the target hybrids 10a-h.      13  N-Benzylindoline-2,3-diones 12a,b Compounds 12a,b were prepared according to the literature procedure 45 .
General procedure for preparation of the target hybrids (13a-c) Following the same procedures described for preparation of the hybrids 5a-h, using N-benzylindoline-2,3-diones 7a,b instead of indoline-2,3-dione 4a-d. Anti-proliferative activity of the prepared hybrids against MDA-MB-231 cells was evaluated by the cell GI assay using 5-Fluorouracil (5-FU) as a standard treatment. This assay was conducted by the use of WST-1 reagent for determination of IC 50 for each compound and the results are given in Table 1. After 48 h of incubation with the synthesized agents in the treatment groups or the vehicle (1% DMSO) in the control group, 10 lL of the WST-1 reagent were added to each well and the plates were re-incubated for 4 h at 37 C. The amount of formazan was quantified using ELISA reader at 450 nm. Moreover, cytotoxicity of the compounds and the reference drug, doxorubicin in LoVo, HepG2 and A549 cells was assessed against control group at the end of exposure using the sulforhodamine B (SRB) assay. Experimental conditions were tested using three replicates (three wells of the 96-well plate per experimental condition) and all experiments were performed in triplicates. IC 50 was calculated according to the equation for Boltzman sigmoidal concentration-response curve using the nonlinear regression fitting models by Graph Pad, Prism version 5 (GraphPad Software Inc., La Jolla, CA).

ELISA immunoassay
Effects of treatment of MDA-MB-231 cells with the most promising compounds 5e and 10g on the cells' apoptotic machinery were  further investigated. The levels of the apoptotic markers caspase-9, caspase-3 and Bax as well as the anti-apoptotic marker Bcl-2 were assessed using ELISA colorimetric kits (Neogen, Lexington, KY) as per the manufacturer's instructions. MDA-MB-231 cells were cultured as a monolayer in T-25 flasks and were seeded to attain 30% confluency prior to treatment. Cells were then treated separately with compounds 5e and 10g at their IC 50 concentrations (12.35 and 12 lM, respectively) for 48 h. At the end of treatment, cells were collected via trypsinization and centrifuged at 10,000 rpm. The pellet was then rinsed with phosphate buffered saline (PBS) and lysed in radio immunoprecipitation assay (RIPA) lysis buffer at 4 C for 45 min, then centrifuged at 14,000 rpm for 20 min to remove the cellular debris. Lysates were then collected and stored at À80 C for later protein determination using Pierce BCA Protein Assay Kit (Pierce Biotechnology, Rockford, IL) according to manufacturer's recommendations.
The cell lysate was diluted 10 times, and 100 lL (50 mg protein) was added to the wells of four separate microtiter plates for the four ELISA kits that were pre-coated with primary antibodies specific to caspase-9, caspase-3, Bax and Bcl-2 proteins, respectively. A secondary biotin-linked antibody specific to the protein captured by the primary antibody was further added to bind the captured protein, forming a "sandwich" of specific antibodies around the desired protein in the cell lysate. The streptavidin-horseradish peroxidase (HRP) complex was then used to bind the biotin-linked secondary antibody through its streptavidin portion. The HRP domain reacted with the added TMB substrate, forming a colored product that was measured at 450 nm by a plate reader (ChroMate-4300, Awareness Technology, Inc., Palm City, FL) after the reaction was terminated by the addition of stop solution.
Annexin V-FITC apoptosis Apoptotic cells were further analyzed by Annexin V-FITC/DAPI assay (Cayman Chemical, Ann Arbor, MI). Briefly, MDA-MB-231 cells were cultured to a monolayer then treated with compound 10g at the IC 50 concentration (12 lM) as described earlier. Cells were then harvested via trypsinization, and rinsed twice in PBS followed by binding buffer. Moreover, cells were re-suspended in 100 lL of binding buffer with the addition of 1 lL of FITC-Annexin V (Becton Dickinson BD Pharmingen TM , Heidelberg, Germany) followed by an incubation period of 30 min at 4 C. Cells were then rinsed in binding buffer and re-suspended in 150 lL of binding buffer with the addition of 1 lL of DAPI (1 lg/lL in PBS) (Invitrogen, Life Technologies, Darmstadt, Germany). Cells were then analyzed using the flow cytometer BD FACS Canto II (BD Biosciences San Jose, CA) and the results were interpreted with FlowJo7.6.4 software (Tree Star, FlowJo LLC, Ashland, OR).

Statistical analysis
Data are presented as means ± SD. Individual groups were compared using the two-tailed independent student's t-test. Multiple group comparisons were carried out using one-way analysis of variance (ANOVA) followed by the Tukey-Kramer test for post-hoc analysis. Statistical significance was accepted at a level of p < 0.05. All statistical analyses were performed using GraphPad InStat software, version 3.05 (GraphPad Software, Inc., La Jolla, CA). Graphs were sketched using GraphPad Prism software, version 5.00 (GraphPad Software, Inc., La Jolla, CA).

ADME profiling
Absorption, distribution, metabolism and excretion (ADME) profiling for the prepared hybrids was carried out using Discovery Studio 2.5 (Accelrys, San Diego, CA). All the tested hybrids were drawn as a small library then prepared using ligand protocol to find the suitable orientation in 3D. ADME profiling was predicted for the designed library using ADME descriptors protocol.

Chemistry
The synthetic strategies employed to prepare the new target hybrids are depicted in Schemes 1-3. In Scheme 1, 4-chloro-2-phenylquinazolines 2a,b were obtained (in 75 and 72% yield, respectively), by chlorination of 2-phenylquinazolin-4(3H)-ones 1a,b in excess refluxing phosphorous oxychloride. Next, 4-chloro-2-phenylquinazolines 2a,b were refluxed with hydrazine hydrate in ethanol to furnish the hydrazine derivatives 3a,b (in 81 and 86% yield, respectively), which were condensed with different isatins in refluxed ethanol in the presence of a catalytic amount of glacial  acetic to afford the isatin-quinazoline hybrids 5a-h (in 65-77% yield). IR spectra of the latter hybrids displayed absorption bands due to the NH groups in the region 3170-3305 cm À1 , in addition to a carbonyl band in the region 1672-1690 cm À1 . The 1 H NMR spectra of compounds 5a-h confirmed the presence of two D 2 O-exchangeable singlet signals attributable to NH protons of the hydrazine function ( ¼ N-NH-) and the isatin in the range d 10.62-11.49 and 12.11-13.93 ppm, respectively.
For the synthesis of isatin-phthalazine hybrids 10a-h, 2-benzoylbenzoic acids 6a,b were cyclocondensed with hydrazine sulfate in the presence of sodium hydroxide to afford phthalazinone 7a,b. Next, chlorination of compounds 7a,b was carried out via refluxing with excess phosphorus oxychloride to furnish 1-chloro-4-phenylphthalazines 8a,b (in 65 and 72% yield, respectively). The chloro intermediates 8a,b were reacted with hydrazine hydrate in refluxing ethanol to provide the 1-hydrazinyl-4-phenylphthalazines 9a,b (in 84 and 80% yield, respectively), which upon  reaction with different isatins afforded the target hybrids 10a-h (in 60-75% yield) (Scheme 2). IR spectra of 10a-h revealed the presence of an NH stretching band about 3200 cm À1 . Their 1 H NMR spectra showed two D 2 Oexchangeable singlet signals from the hydrazine function ( ¼ N-NH-) and the isatin protons in the range d 10.56-10.74 and 12.83-13.17 ppm.
Finally, isatin was refluxed with benzyl bromide or 4-fuorobenzyl bromide in dry DMF and anhydrous K 2 CO 3 to furnish N-substituted isatins 12a,b, respectively (in 88 and 82% yield, respectively). The reaction of derivatives 12a,b with the appropriate 4-hydrazinyl-2-phenylquinazoline derivative 3 in ethanol in the presence of a catalytic amount of glacial acetic acid afforded the target hybrids 13a-c (Scheme 3).
The structures of hybrids 13a-c were confirmed under the basis of spectral and elemental analyses which were in full agreement with the proposed structures.

Biological evaluation
In vitro anti-proliferative activity against TNBC MDA-MB-231 cell line Anti-proliferative activity of the newly prepared hybrids 5a-h, 10a-h and 13a-c was evaluated against MDA-MB-231 TNBC cell line using the WST-1 assay as described by Ngamwongsatit et al. 50 . Being a well-known broad-spectrum anticancer drug and a first line therapy for many tumors management, 5-FU was chosen as a positive control 51 , 52 . The anti-proliferative activity was expressed as the half maximal inhibitory concentration (IC 50 ) values (Table 1).
From the obtained results, it is obvious that most of the tested hybrids have excellent to moderate growth inhibitory activity toward the tested MDA-MB-231 cancer cell line. In particular, compounds 5e and 10g were the most active members against MDA-MB-231 cells (IC 50 ¼ 12.35 ± 0.12 and 12.00 ± 0.13 lM, respectively), with 2.37-and 2.44-fold increased activity than the reference drug, 5-FU (IC 50 ¼ 29.38 ± 1.24 lM). Besides, compounds 5a-d, 5f-h, 10a-f, 10h and 13a displayed good anti-proliferative activity with IC 50 values ranging from 12.86 ± 0.12 to 26.21 ± 2.07 lM. Whilst, hybrids 13a and 13b were moderately active with IC 50 values of 48.72 ± 2.59 and 34.17 ± 2.42 lM, respectively.

Structure activity relationship SAR
By scrutinizing the aforementioned biological data, one can snugly reveal a clear structure activity relationship. First, we investigated the impact of the substitution at the 5-position of the isatin moiety. Regarding the isatin-quinazoline hybrids 5a-h, incorporation of unsubstituted isatin resulted in compounds 5a and 5e with moderate activity against the MDA-MB-231 cell line (IC 50 ¼ 13.18 ± 0.36 and 12.35 ± 0.12 lM, respectively). As it has an electronic and size properties similar to those of hydrogen, fluorine is introduced as a classical bioisostere to the hydrogen atom. Compounds 5b and 5f bearing fluorine substituent, displayed slight decrease in the anti-proliferative activity (IC 50 ¼ 15.15 ± 0.15 and 12.95 ± 0.31 lM, respectively) compared to the unsubstituted analogs, suggesting that halogens incorporation may not be advantageous for the activity. Furthermore, introduction of more bulky and lipophilic chlorine atom; compounds 5c and 5g, caused decrease of activity against MDA-MB-231 cells (IC 50 ¼ 17.9 ± 0.11 and 14.59 ± 0.23 lM, respectively). Also, incorporation of bromine atom (more bulky and lipophilic than chlorine) decreased the antiproliferative activity (IC 50 ¼ 19.68 ± 0.26 and 17.31 ± 0.55 lM, respectively). Thus, the order of activity of the halogenated hybrids in such series, were smoothly decreased in the order of F > Cl > Br, hinting that increasing the lipophilicity and the size of the substituents of isatin moiety at the 5-position is not affirmative for the activity of the isatin-quinazoline hybrids regardless of the type of substitution of the 2-phenyl group of the quinazoline moiety.
Regarding the isatin-phthalazine hybrids 10a-h, the abovementioned relationship could be, also, proposed for the hybrids 10a-d with unsubstituted 4-phenyl group in the phthalazine moiety. While, substitution of hybrids 10e-h (containing 4-(4-chlorophenyl) group) with F or Cl at 5-position of isatin moiety has no significant effect, introduction of Br led to a decreased activity toward the MDA-MB-231 cell line.
In conclusion, we can deduce that unsubstitution of isatin moiety at C-5 or N-1 positions, disubstitution of the 2-phenyl group of quinazoline moiety and substitution of the 4-phenyl group of phthalazine moiety are crucial elements for the anti-proliferative activity against MDA-MB-231.
Effects on mitochondrial apoptosis pathway proteins Bax and Bcl-2 Induction of apoptosis in cancer cells is one of the successful strategies for the development of cancer therapy [53][54][55] . Therefore, we investigated the potential pro-apoptotic effects of our isatin-quinazoline and isatin-phthalazine hybrids attempting to explore the underlying mechanism for their anti-proliferative activity.
As indicated by the cytotoxicity results, compounds 5e and 10g were found to be the most active agents against MDA-MB-231 breast cancer cells. Thus, we investigated the potential proapoptotic effects of these promising agents in an attempt to define the principle mechanism for their anti-proliferative activity.
The Bcl-2 family of proteins is responsible for synchronizing the mitochondrial apoptotic pathway 56 . These proteins are classified into two groups: anti-apoptotic proteins such as Bcl-2 protein and the counteracting pro-apoptotic proteins including Bax protein 57 .
It has been previously indicated that the overexpression of the anti-apoptotic Bcl-2 protein is a contributing factor to tumorigenesis in a multitude of cancers including breast cancer 58 . In this study, exposure of MDA-MB-231 breast cancer cells to compounds 5e and 10g for 48 h resulted in a significant increase in the expression of the pro-apoptotic protein Bax by $130 and 180%, respectively, compared to the control (Figure 3(A), Table 2). On the other hand, treatment of MDA-MB-231 breast cancer cells with compounds 5e and 10g significantly reduced the expression levels of the anti-apoptotic protein Bcl-2 by $29 and 52%, respectively, compared to the control (Figure 3(B), Table 2). A rather more important parameter is the ratio between Bax (apoptosis inducer) and Bcl-2 (apoptosis suppressor) that gave more real insight to the apoptotic activity of the compounds as it is considered a key indicator of therapeutic response to chemotherapy. Analyzing the results discloses that compound 5e increased the Bax/Bcl-2 ratio two folds compared to the control, while compound 10g even boosted it four folds in comparison to the control. The ability of the two compounds to down regulate Bcl-2 levels while boosting Bax levels further supports their effectiveness as apoptosis inducers.
Effects on the level of active caspase-9 and caspase-3 The down-regulation of the anti-apoptotic Bcl-2 results in increased levels of free pro-apoptotic Bax which then accumulates at the inner mitochondrial membrane forming channels thus altering membrane permeability. Apoptotic factors then leak into the cytoplasm resulting in the activation of caspases cascade 59 . Caspases are cysteine-containing aspartic acid-specific proteases, which exist as zymogens in the cytoplasm and play a pivotal role in the execution of cell death upon activation 60 . Caspase-3 is the key executioner protease that is activated by upstream initiator caspases such as caspase-9 60 . Therefore, the elevated Bax/Bcl-2 ratios obtained with these treatments triggered the investigation of the protein expression levels of active caspases-9 and -3.
In this study, treatment of MDA-MB-231 cells with compounds 5e and 10g resulted in a significant elevation of active caspase-9 protein levels by around 2.3 and 2.7 folds, respectively, compared to control (Figure 4(A), Table 2). Moreover, MDA-MB-231 cells upon treatment with compounds 5e and 10g, exhibited a significant upregulation of active caspase-3 protein levels by $2.4 and 3.7 folds, respectively, compared to control (Figure 4(B), Table 2).
It is worth noting that upon comparing the relative expression levels of caspases with the relative elevation patterns of Bax/Bcl-2 ratio observed in both treatments, it was found that the magnitude of the Bax/Bcl-2 ratio elevation is proportional to that of caspase-9 and that of caspase-3 up-regulation. This observation strengthens the correlation between the increased Bax/Bcl-2 ratio and the activation of proteolytic caspases that act as key players in the execution of apoptosis, which might be responsible for the anti-proliferative activity of the tested compounds. Moreover, compound 10g exhibited a more potent activity than compound 5e in terms of both elevating Bax/Bcl-2 ratio and further activating the caspases cascade at a relatively lower IC 50 . This finding directed further investigation of the apoptotic effect of this compound on MDA-MB-231 cells.
Annexin V-FITC apoptosis assay Externalization of the phospholipid phosphatidylserine (PS) at the cell membrane is one of the hallmarks of cells going into apoptosis 61 . Having a high Ca 2þ -dependent binding affinity to negatively charged phospholipid surfaces. Annexin A5 (AnxV A5), a 36-kDa human protein, is a suitable candidate for apoptosis imaging. Besides, Anx V-based flow cytometry analysis is a useful tool to  comprehend whether cell death is due to physiological apoptosis or nonspecific necrosis 61 . Further evaluation of the apoptotic effect of the most potent compound 10g was carried out using Anx V-FITC/DAPI dual staining assay ( Figure 5). MDA-MB-231 cells treated with compound 10g showed a significant increase in the percent of Anx V-FITC positive apoptotic cells (apoptotic and late apoptotic) from 3.88 to 31.21% which comprises about 8.4-folds (p < 0.001) compared to control ( Figure 5). Flow cytometric analysis of the differential binding of the cells to Anx V-FITC displayed a significant increase in the proportion of early apoptotic cells. This observation is in line with the simultaneous up-regulation of the upstream caspase-9 (an initiator caspase) together with the downstream caspase-3 (the hallmark key executor) belonging to the intrinsic apoptotic pathway. This strongly suggests as well that a cascade of proteinases' activation has been triggered as a consequence of an increase in Bax/Bcl-2 ratio, eventually leading to apoptosis. These findings clearly imply that compound 10g has potent pro-apoptotic activity that unravels the mechanism of its anti-proliferative activity.
In conclusion, the reduced expression of the anti-apoptotic protein Bcl-2 in addition to the enhanced expression of the proapoptotic protein Bax as well as the up-regulated active caspase-9 and caspase-3 levels together with a harmonized increase in the Bax/Bcl-2 ratio, suggests that the cytotoxic effect of compounds  5e and 10g might be attributed, at least in part, to the induction of the intrinsic apoptotic mitochondrial pathway.
In vitro anti-proliferative activity against four different cell lines The in vitro anti-proliferative activity of the prepared hybrids 5a-h, 10a-h and 13a-c was further examined against a panel of cell lines, namely A549 alveolar carcinoma, Caco-2 colon cancer, LoVo human colorectal carcinoma and HepG2 hepatocellular carcinoma using SRB colorimetric assay as described by Skehan et al. 62 , to carry out further elaboration for their anti-proliferative activity. The results were expressed as IC 50 values and listed in Table 3.
The presented data revealed that some hybrids displayed moderate to fair anti-proliferative activity toward A549 cell line. In particular, compounds 5d and 10d showed the better activity against A549 with IC 50 values of 28.73 ± 1.59 and 18.42 ± 1.52 lM, respectively. Besides, most compounds possessed good to moderate activity against LoVo cell line with IC 50 values ranged from 13.09 ± 0.33 to 48.62 ± 2.21 lM. Regrettably, among the tested cancer cell lines, the colon Caco-2 and hepatocellular HepG2 were not susceptible cell lines to almost all hybrids influence. ADME study The ADME of the prepared hybrids 5a-h, 10a-h and 13a-c was predicted through a theoretical kinetic study performed by Discovery Studio software (Table 4). To evaluate the lipophilicity and polar surface area, AlogP98 and PSA_2D descriptors were calculated. Besides, absorption, solubility and CYP2D inhibition levels were predicted. All compounds were expected to have very low-to-low aqueous solubility. Meanwhile, most of the examined derivatives seemed to possess good to poor absorption levels, and predicted to be CYP2D inhibitors except compounds 10a, 10e-h and 13b which are expected not to inhibit CYP2D.

Conclusion
In an effort to develop potent anti-proliferative agents, the molecular hybridization approach was adopted to design and synthesize new different series of isatin-quinazoline hybrids 5a-h, isatin-phthalazine hybrids 10a-h and N-benzylisatin-quinazoline hybrids 13a-c. The in vitro anti-proliferative activity of the newly synthesized hybrids was evaluated against the TNBC MDA-MB-231 breast cancer. Compounds 5e and 10g displayed the highest potency toward MDA-MB-231 with IC 50 values of 12.35 ± 0.12 and 12.00 ± 0.13 lM, respectively. Subsequently, compounds 5e and 10g were further estimated for their apoptosis induction potential. Both compounds 5e and 10g proved to induce apoptosis, which was assured by the reduced expression of the anti-apoptotic protein Bcl-2 in addition to the enhanced expression of the pro-apoptotic protein Bax as well as the upregulated active caspase-9 and caspase-3 levels together with a harmonized increase in the Bax/Bcl-2 ratio. Furthermore, compound 10g showed a significant increase in the percent of annexin V-FITC positive apoptotic cells from 3.88 to 31.21% which comprises about 8.4 folds compared to control. Finally the Solubility parameter. (0:À2 ¼ optimal, À2:À4 ¼ good, À4:À6 ¼ low, À6:À8 ¼ very low). d Solubility level (0 ¼ extremely low, 1 ¼ very low but possible, 2 ¼ low, 3 ¼ good, 4 ¼ optimal). e Absorption level (0 ¼ good, 1 ¼ moderate, 2 ¼ low, 3 ¼ very low). f CYP2D inhibition (0 ¼ non inhibitor, 1 ¼ inhibitor). Table 3. In vitro anti-proliferative activities of the newly synthesized hybrids against A549, Caco-2, LoVo and HepG2 cell lines.
newly prepared hybrids were examined against a panel of cell lines, namely, LoVo human colorectal carcinoma, A549 alveolar carcinoma and Caco colon cancer cell lines to carry out further elaboration for their anti-proliferative activity. Based on the previous findings, we came into conclusion that the isatin-quinazoline/phthalazine hybrids are a good platform for further optimization as novel anticancer agents for TNBC.