Phenolic profile, antioxidant, anticholinesterase, and anti-tyrosinase activities of the various extracts of Ferula elaeochytris and Sideritis stricta

ABSTRACT In this study, the antioxidant, anticholinesterase, and anti-tyrosinase properties of (hexane, acetone, methanol, and water) extracts of Ferula elaeochytris and Sideritis stricta were determined with the total phenolic and flavonoid contents. The phenolic profile of the methanol and water extracts was analysed using HPLC-DAD. Protocatechuic acid was found as the major phenolic compound in the methanol (116.3 ± 3.1 µg/g) and water extracts (69.4 ± 1.3 µg/g) of F. elaeochytris. Coumarins (253.9 ± 4.1 µg/g) and catechin hydrate (175.2 ± 2.9 µg/g) were the most abundant phenolic compounds in the methanol and water extracts of S. stricta. β-carotene–linoleic acid, DPPH•, ABTS•+, CUPRAC, and metal-chelating assays were used to evaluate antioxidant properties of the extracts. The methanol and water extracts of F. elaeochytris and the acetone and methanol extracts of S. stricta containing the highest amount of total phenolic and flavonoid contents showed the highest antioxidant activities in β-carotene–linoleic acid, DPPH•, ABTS•+, and CUPRAC assays. The enzyme inhibitory potential of extracts was investigated against key enzymes involved in neurodegenerative (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)) and skin (tyrosinase) disorders. In the cholinesterase inhibitory assays, the hexane extracts of two species exhibited the best activity against AChE, while the hexane extract of F. elaeochytris and the methanol extract of S. stricta observed to be the most active against BChE. As for anti-tyrosinase activity results of extracts, the only acetone and methanol extracts showed mild inhibitory activity for both species.


Introduction
The Apiaceae family (formerly Umbelliferae) comprises of flowering and aromatic plants mostly growing in temperate areas. The Ferula genus belongs to the family of Apiaceae, consists of 170 species which spreads from Central Asia to Mediterranean region. In Turkey, the Ferula genus is one of the most important genera and represented by 25 taxa. Ferula species are known as 'Çakşır', 'Çakşır otu' or 'Çaşır' in Turkey. [1] The herbal parts of Ferula species are used as animal feed in winter months, while the root parts are used as an aphrodisiac in Eastern Turkey. The Ferula genus was reported as a rich source of gum resin. Therefore, this genus is used in the treatment of many diseases such as digestive disorders, rheumatism, headache, arthritis, toothache, and diabetes in folk medicine. Some species are used as traditional foods and a flavouring agent as well as a traditional medicine for many diseases in many parts of world. [2] Ferula species is a rich source of biologically as following: (i) F. elaeochytris: Aydıntepe county, Gümüşdamla village (1650 m altitude), Bayburt, Turkey; 2016 (Voucher no: MUMED1051). (ii) S. stricta: Fethiye-Antalya road, Karabel plateau (1300 m altitude), Muğla, Turkey; 2016 (Voucher no: MUMED1121).

Extraction
The aerial parts of F. elaeochytris (120 g) and S. stricta (140 g) were extracted separately with different solvents (2.5 L) according to their increasing polarity: hexane, acetone, and methanol at room temperature for 24 h and four times. Solvents were evaporated on a rotary evaporator to obtain hexane, acetone, and methanol extracts. The remaining plant part was allowed to stand for one day with water at 80°C. The water extract was obtained by lyophilization using a freeze-drier. All extracts were stored at +4°C until analysis.

Total phenolic and flavonoid contents
The phenolic content of extracts was stated as microgram of pyrocatechol equivalents (PEs). [22] The phenolic contents were calculated according to the following equation that was obtained from standard pyrocatechol graph: Absorbance ¼ 0:0209 pyrocatechol μg ð Þ ½ þ 0:0315 r 2 ; 0:9995 À Á : Measurement of flavonoid content of the extracts was based on the aluminium nitrate method, and results were expressed as microgram of quercetin equivalents (QEs). [23] The flavonoid contents were calculated according to the following equation that was obtained from the standard quercetin graph: Absorbance ¼ 0:0657 quercetin μg ð Þ ½ À 0:0195 r 2 ; 0:9999 À Á :

Antioxidant activity
β-carotene/linoleic acid assay The total antioxidant activity was determined by β-carotene-linoleic acid method based on the measurement of the inhibition of conjugated dien hydroperoxides resulting from linoleic acid oxidation with slight modifications. [26] A β-carotene-linoleic acid mixture was prepared as following: 0.5 mg β-carotene in 1 mL of chloroform was added to 25 μL linoleic acid and 200 mg Tween 40 emulsifier mixture. After evaporation of chloroform under vacuum, 100 mL of distilled water saturated with oxygen was added by vigorous shaking. One hundred and sixty microliters of this mixture were transferred into 40 μL of the samples at different concentrations. As soon as the emulsion was added into each tube, the zero time absorbance was measured at 470 nm using a 96well microplate reader. Absorbance of the emulsion was read again at the same wavelength after incubation of the plate for 2 h at 50°C. Measurement of absorbance was continued until the colour of β-carotene disappeared. BHA and α-tocopherol were used as antioxidant standards for comparison of the activity. The same procedure was repeated with used antioxidant standards and a blank. The bleaching rate (R) of β-carotene was calculated according to Eq. (1).
where ln = natural log, a = absorbance at time zero, and b = absorbance at time t (120 min). Antioxidant activity was calculated in terms of percentage inhibition relative to the control, using Eq. (2).
DPPH free radical scavenging assay The free radical scavenging activity was determined spectrophotometrically by the DPPH assay described by Blois [27] with slight modification. [26] In its radical form, DPPH absorbs at 517 nm, but upon reduction by an antioxidant or a radical species, its absorption decreases. Briefly, 40 μL sample solutions at different concentrations were added to 160 μL 0.4 mM 40 μL methanol solution of DPPH. Thirty minutes later, absorbance was measured at 517 nm by using a 96-well microplate reader. The capability of scavenging the inhibition activity (I) was calculated using Eq. (3).

ABTS cation radical scavenging assay
The spectrophotometric analysis of ABTS •+ scavenging activity was determined according to the method of Re et al. [28] with slight modifications. [26] Briefly, ABTS •+ was produced by the reaction between 7 mM ABTS in H 2 O and 2.45 mM potassium persulfate, stored in the dark at room temperature for 12 h. The radical cation was stable in this form for more than 2 days when stored in the dark at room temperature. Before usage, the ABTS •+ solution was diluted to get an absorbance of 0.708 ± 0.025 at 734 nm with ethanol. Then, 160 µL of ABTS •+ solution was added to 40 µL of sample solution in ethanol at different concentrations. After 10 min, by using a 96-well microplate reader, the percentage inhibition at 734 nm was calculated for each concentration relative to a blank absorbance (ethanol). The scavenging capability of ABTS •+ was calculated using Eq. (3).

Cupric-reducing antioxidant capacity (CUPRAC) assay
The cupric-reducing antioxidant capacity was determined according to the method of Apak et al. [29] with slight modifications. [26] To each well, in a 96-well plate, 50 µL 10 mM Cu (II), 50 µL 7.5 mM neocuproine, and 60 µL NH 4 Ac buffer (1 M, pH 7.0) solutions were added. Forty microliters of extract at different concentrations were added to the initial mixture so as to make the final volume to 200 µL. After 1 h, the absorbance at 450 nm was recorded against a reagent blank by using a 96-well microplate reader.

Metal chelating assay
The chelating activity of the extracts on Fe 2+ was measured as reported by Decker and Welch [30] with slight modifications. [31] About 80 µL extract solution dissolved in ethanol in different concentrations was added to 40 µL 0.2 mM FeCl 2 . The reaction was initiated by the addition of 80 µL 0.5 mM ferene. The mixture was shaken vigorously and left standing at room temperature for 10 min. After the mixture reached equilibrium, the absorbance was measured at 593 nm. The metal chelating activity was calculated according to Eq. (3).

Enzyme inhibitory activity
Cholinesterase inhibition Acetylcholinesterase and butyrylcholinesterase inhibitory activity was measured by the spectrophotometric method developed by Ellman [32] as previously reported with slight modification. [26] AChE from electric eel and BChE from horse serum were used, while acetylthiocholine iodide and butyrylthiocholine chloride were employed as substrates of the reaction. DTNB

Tyrosinase inhibition
Tyrosinase enzyme inhibitory activity was measured by the spectrophotometric method as described by Masuda et al. [33] with slight modification. Mushroom tyrosinase was used, while L-DOPA was employed as substrates of the reaction. Briefly, 150 µL of 100 mM sodium phosphate buffer (pH 6.8), 10 µL of sample solution dissolved in ethanol at different concentrations, and 20 µL tyrosinase enzyme solution in buffer were mixed and incubated for 10 min at 37°C, and 20 µL L-DOPA was added. The sample and blank absorbances were read at 475 nm after 10 min incubation at 37°C in a 96-well microplate.

Statistical analysis
All data on antioxidant, anticholinesterase, and anti-tyrosinase activity tests were the average of three parallel sample measurements. Data were recorded as mean ± S.E.M. Significant differences between means were determined by Student's test, and p values <0.05 were regarded as significant.

Results and discussion
Total phenolic and total flavonoid contents The total phenolic and flavonoid contents of the extracts of F. elaeochytris and S. stricta were determined as pyrocatechol and QEs, respectively, and the results are presented in Table 1. The concentration of phenolic compounds in the extracts ranged from 1.77 ± 0.18 to 141.05 ± 0.11 µg PEs/mg. The highest level of phenolic compounds was measured in the acetone extract (141.05 ± 0.11 µg PEs/mg) and followed by the methanol extract (65.40 ± 0.32 µg PEs/mg) of S. stricta. The acetone extract (21.62 ± 0.73 µg PEs/mg) of F. elaeochytris exhibited the highest concentration of phenolic compounds, followed by the water extract (19.23 ± 0.21 µg PEs/mg). The total flavonoid contents in the examined extracts ranged from 0.62 ± 0.32 to 50.52 ± 0.59 µg QEs/mg. The highest level of flavonoid contents was measured in the acetone extract (50.52 ± 0.59 µg QEs/mg) and followed by the methanol extract (26.21 ± 0.39 µg QEs/mg) of S. stricta. The acetone extract (10.33 ± 0.23 µg QEs/mg) of F. elaeochytris showed the highest concentration of flavonoid compounds, followed by the methanol extract (9.93 ± 0.56 µg QEs/mg) ( Table 1).

Phenolic profiles
Phenolic and organic acid compounds of the methanol and water extracts of F. elaeochytris and S. stricta were determined by HPLC-DAD, and results are expressed as µg/g extract in Table 2. Totally 16 phenolic and organic acid compounds namely fumaric acid, gallic acid, protocatechuic acid, p-hydroxybenzoic acid, catechin hydrate, 6,7-dihydroxy coumarin, 2,4-dihydroxybenzoic acid, caffeic acid, vanillin, p-coumaric acid, ferulic acid, coumarins, trans-2-hydroxycinnamic acid, ellagic acid, rosmarinic acid, and trans-cinnamic acid were identified in the extracts. Protocatechuic acid was identified as a dominant phenolic compound in both extracts of F. elaeochytris (Fig. 1). Protocatechuic acid has antioxidant and anticancer activities, so high antioxidant activities of the methanol and water extracts are associated with high amount of protocatechuic acid. [34] Also, the second major phenolic compound was catechin hydrate (105.5 ± 2.3 µg/g) in the methanol extract of F. elaeochytris and fumaric acid (52.5 ± 1.0 µg/g) in the water extract of F. elaeochytris. In the methanol extract of S. stricta, coumarins (253.9 ± 4.1 µg/g) were found as the major phenolic compound, while catechin hydrate (175.2 ± 2.9 µg/g) was found to be the major phenolic compound in the water extract. Caffeic acid was identified as the second major phenolic compound in both extracts (Fig. 2). It was reported that coumarins, catechin hydrate, and caffeic acid possess higher antioxidant activity when compared to BHA and α-tocopherol. [35,36] This may cause two extracts to exhibit higher antioxidant activity than standards. Consequently, both studied species were rich in phenolic compounds.

Antioxidant activity
Five types of antioxidant capacity measurements, namely β-carotene-linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, cupric-reducing antioxidant capacity (CUPRAC), and metal chelating activity, were used to test antioxidant properties of the hexane, acetone, methanol, and water extracts of F. elaeochytris and S. stricta species. All of the extracts exhibited antioxidant activities in a dose-dependent manner. Table 3 shows the IC 50 values of the extracts and standard compounds (α-tocopherol, BHA, and EDTA).
Earlier studies have shown that the acetone and methanol extracts of S. brevibracteata have high activity in DPPH • assay and moderate activity in β-carotene-linoleic acid and CUPRAC assay [42] ; the methanol extracts of S. ozturkii, S. caesarea, and S. montana have high activity in DPPH • assay. [43,44] In the study of Zengin et al., [45] antioxidant properties of the petroleum ether, ethyl acetate, methanol, and water extracts of S. galactica were tested using free radical scavenging (DPPH, ABTS, and NO), reducing power (FRAP and CUPRAC), total antioxidant capacity, and chelating assays. It was determined that the methanol and water extracts had high radical scavenging and reducing power activities. The findings we obtained are completely in line with previous studies.
Phenolic compounds are the most important compound groups of plants that are capable of scavenging ability on free radicals by hydroxyl groups. Flavonoid compounds are important phenolic compounds showing antioxidant properties. [46] The methanol and water extracts were found to be rich in protocatechuic acid, coumaric acid, catechin hydrate, and caffeic acid which were previously confirmed as good antioxidant agents. [34][35][36] Furthermore, the highest antioxidant activities of the acetone, methanol, and water extracts of F. elaeochytris and S. stricta can be explained by the highest amount of total phenolic and flavonoid compounds.

Anticholinesterase activity
There are many drugs used in the treatment of AD, and these drugs have some side effects. Therefore, it is necessary to obtain new inhibitor for AD which is less toxic. Today, there are a limited number of inhibitors (galantamine, tacrine, and physostigmine) that are derived from natural plant sources and reduce the effects of AD. [47,48] Scientists' interest in the finding of new natural sources of medicines for the treatment of AD is growing recently. Table 4 summarizes the results of cholinesterase enzyme inhibitory activities of the extracts of F. elaeochytris and S. stricta. The inhibitory activities of the extracts on AChE and BChE were reported as potent (>50%), moderate (30-50%), inactive, or low (<30%) activity. [49] According to this classification, the hexane extract of F. elaeochytris (70.66 ± 0.93%) showed potent inhibitory activity against AChE. The inhibitory activities of the acetone and methanol extracts of F. elaeochytris on AChE were found to be low, and the water extract exhibited no inhibition ( Table 4). The BChE inhibitory activities of the extracts were higher than the AChE inhibitory activities. The hexane extract of F. elaeochytris showed higher BChE inhibitory activity with IC 50 values of 49.80 ± 0.07 µg/mL when compared to galantamine (IC 50 : 50.80 ± 0.93 µg/mL) ( Table 4). In previous reports, anti-AChE activities of the ethanolic extract of F. hermonis, the methanol extract of F. assafoetida, and the ethyl acetate and n-butanol extracts of F. lutea were studied, and all extracts showed low ability for inhibiting acetylcholinesterase. [37,41,50] There is no study about anti-BChE activity of the extracts from Ferula species, so this the first study about anti-BChE activity.
The hexane extract of S. stricta (58.59 ± 0.25%) showed potent inhibitory activity against AChE. When the acetone and methanol extracts of S. stricta showed low AChE inhibitory activity, water extract exhibited no AChE inhibitory activity. Against BChE, the hexane and acetone extracts of S. stricta were found as potent inhibitors. (Table 4). There are only two studies in the literature about cholinesterase inhibition activity of Sideritis extracts. According to the results obtained by Miguel et al. [51] the ethanolic extract of S. arborescens had no ability for inhibiting acetylcholinesterase. Zengin et al. [45] reported that cholinesterase enzyme inhibitory activities of the extracts of S. galactica were decreased in order of ethyl acetate > petroleum ether > methanol > water extracts.
When studies on the extracts of Ferula and Sideritis species are examined, it is seen that nonpolar extracts have higher anticholinesterase activity than polar extracts. As it seen in Table 4, polar extracts of both species showed lower activity, so our results are consistent with the literature data. The highest cholinesterase inhibitory activities of the hexane extracts in both species studied can be explained by their non-phenolic composition.

Anti-tyrosinase activity
Over the past years, tyrosinase inhibitors are gaining importance due to the impact of tyrosinase on the human melanogenesis and plant and fungi browning. In the method used, tyrosinase enzyme inhibitory activity is based on the measurement of dopachrome formed in the presence of tyrosinase and L-DOPA as the enzyme substrate. [52] As it seen in Table 4, the methanol (24.63 ± 0.31%) and acetone extracts (7.14 ± 0.08%) of F. elaeochytris displayed low tyrosinase enzyme inhibition activity, while the hexane and water extracts showed no activity. The methanol extracts of 13 plant species belonging to Umbelliferae family were screened for their tyrosinase inhibitory effects. According to Orhan et al., [53] all methanol extracts showed low tyrosinase inhibitory activity.
The methanol (23.29 ± 0.56%) and acetone (15.66 ± 0.11%) extracts of S. stricta were found to be low active against tyrosinase, while the hexane and water extracts were found to be inactive (Table 4). Previously, tyrosinase inhibitory activities of plants that are members of Lamiaceae family were investigated. Tundis et al. [54] screened tyrosinase inhibitory activities of the hexane, dichloromethane, methanol, and ethanol extracts of Stachys lavandulifolia aerial parts. The result of this study is that all extracts have low tyrosinase inhibitory activity. Alimpic et al. [55] reported IC 50 values of tyrosinase inhibition of the ethanol extracts of Mentha species ranged between 108 ± 20 and 286 ± 45 μg/mL. The obtained data support the results of the literature. Tyrosinase inhibitory activities of the hexane, acetone, methanol, and water extracts of F. elaeochytris and S. stricta species were evaluated for the first time in this study.

Conclusion
In the present study, antioxidant, anticholinesterase, and tyrosinase inhibitory properties of various extracts of F. elaeochytris and S. stricta were determined with the total phenolic and flavonoid contents. The methanol and water extracts of F. elaeochytris and the acetone and methanol extracts of S. stricta containing the highest amount of total phenolic and flavonoid contents showed the highest antioxidant activities in β-carotene-linoleic acid, DPPH • , ABTS •+ , and CUPRAC assays. The hexane extracts of both plant species with the lowest amount of total phenolic and flavonoid contents were found as potent inhibitors in cholinesterase enzyme inhibitory activity assay. Totally, 15 phenolic compounds were identified in the methanol and water extracts by using HPLC-DAD. Protocatechuic acid, coumarins, and catechin hydrate were found to be major phenolic compounds. This study represents the first report on phytochemical composition and biological activities of F. elaeochytris and S. stricta. Our results showed that Ferula and Sideritis species have promising activities and high phytochemical contents. They could be used as a potential source of natural antioxidant, anticholinesterase, and anti-tyrosinase agent in food, cosmetic, and pharmaceutical industries. Thus, further studies are needed to isolate and identify the antioxidant components from these species.