Crystalline silica-induced pulmonary inflammation and autoimmunity in mature adult NZBW/f1 mice: age-related sensitivity and impact of omega-3 fatty acid intervention

Abstract Objective Occupational exposure to respirable crystalline silica (cSiO2) has been linked to lupus development. Previous studies in young lupus-prone mice revealed that intranasal cSiO2 exposure triggered autoimmunity, preventable with docosahexaenoic acid (DHA). This study explores cSiO2 and DHA effects in mature lupus-prone adult mice, more representative of cSiO2-exposed worker age. Methods Female NZBWF1 mice (14-week old) were fed control (CON) or DHA-supplemented diets. After two weeks, mice were intranasally instilled saline (VEH) or 1 mg cSiO2 weekly for four weeks. Cohorts were then analyzed 1- and 5-weeks postinstillation for lung inflammation, cell counts, chemokines, histopathology, B- and T-cell infiltration, autoantibodies, and gene signatures, with results correlated to autoimmune glomerulonephritis onset. Results VEH/CON mice showed no pathology. cSiO2/CON mice displayed significant ectopic lymphoid tissue formation in lungs at 1 week, increasing by 5 weeks. cSiO2/CON lungs exhibited elevated cellularity, chemokines, CD3+ T-cells, CD45R + B-cells, IgG + plasma cells, gene expression, IgG autoantibodies, and glomerular hypertrophy. DHA supplementation mitigated all these effects. Discussion The mature adult NZBWF1 mouse used here represents a life-stage coincident with immunological tolerance breach and one that more appropriately represents the age (20–30 yr) of cSiO2-exposed workers. cSiO2-induced robust pulmonary inflammation, autoantibody responses, and glomerulonephritis in mature adult mice, surpassing effects observed previously in young adults. DHA at a human-equivalent dosage effectively countered cSiO2-induced inflammation/autoimmunity in mature mice, mirroring protective effects in young mice. Conclusion These results highlight life-stage significance in this preclinical lupus model and underscore omega-3 fatty acids’ therapeutic potential against toxicant-triggered autoimmune responses.


Introduction
Systemic lupus erythematosus (lupus) is a devastating systemic autoimmune disease affecting multiple organ systems.Lupus is associated with unresolved inflammation, aberrant exposure to self-antigens leading to the breach of immunologic tolerance, autoreactive B-and T-cell expansion, and a robust pathogenic autoantibody (AAb) response (Tsokos 2011;Yu et al. 2021).Resulting AAbs bind their respective autoantigens (AAgs), forming immune complexes that are distributed via the systemic circulation and deposited in various organs such as the kidney.This process chronically reoccurs, manifesting in cyclical bouts of inflammation and autoimmunity known as lupus flares that impact the quality of life and increase morbidity and mortality in lupus patients (Adamichou and Bertsias 2017).Importantly, repeated flaring promotes the development of glomerulonephritis and irreparable damage to the kidney, eventually leading to end-stage renal failure (Yu et al. 2017).
Lupus susceptibility is strongly influenced by heredity (Ramos et al. 2010).However, environmental exposures to toxicants, infections, UV radiation, and certain medications are linked to lupus onset, flaring, and progression (Wu et al. 2020;Zucchi et al. 2022).Importantly, inhalation of respirable crystalline silica (cSiO 2 ) exposure in the workplace has been implicated in the occurrence of autoimmune diseases such as lupus, rheumatoid arthritis, scleroderma, and ANCA-associated vasculitis (Parks et al. 1999(Parks et al. , 2017;;Hoy and Chambers 2020).The Occupational Health and Safety Administration (OSHA) estimated that 2.3 million workers practicing stonecutting, construction, ceramics, and mining are exposed to respirable cSiO 2 dust(https://www.osha.gov/silica-crystalline).Significantly, lupus risk has been shown to increase with the duration of exposure for women of color in these occupations (Finckh et al. 2006).
Animal models are critical to understanding how the interplay between genes and the environment influences the onset of lupus in humans.The NZBWF1 mouse, which has a genetic predisposition to autoimmune disease, has been a widely utilized preclinical lupus model for over five decades to investigate mechanisms underlying disease development, the impact of environmental factors, and the effectiveness of pharmacological and immunotherapeutic treatments (Dubois et al. 1966;Crampton et al. 2014;Celhar and Fairhurst 2017).Unexposed female NZBWF1 mice spontaneously begin to lose immunologic tolerance and produce AAb at 16-20 weeks of age and develop glomerulonephritis as they age into adults at approximately 32-34 weeks of age (Richard and Gilkeson 2018), significantly earlier than their male counterparts.Female NZBWF1 mice rarely survive beyond 12 months (Gelfand et al. 1972), mirroring the gender-related patterns in human lupus.
Consistent with human epidemiological studies linking cSiO 2 exposure to autoimmunity, our lab has demonstrated that short-term repeated cSiO 2 instillations in young female NZBWF1 lupus-prone mice beginning at 8 weeks of ageaccelerated disease onset by three months with glomerulonephritis being observed at 20-22 weeks of age (Bates et al. 2015).Before evident kidney disease, cSiO 2 exposure triggers pulmonary ectopic lymphoid tissue (ELT) development, robust AAb production in bronchoalveolar lavage fluid (BALF) and plasma, and proinflammatory gene expression in lung and kidney tissues (Bates et al. 2015(Bates et al. , 2019;;Rajasinghe et al. 2020).Altogether, these studies show that inhalation of cSiO 2 leads to extensive pulmonary inflammation, creating a nexus for a local autoimmune response leading to systemic autoimmunity and downstream renal pathology.
Diet is an additional potential environmental factor that can impact lupus onset and progression.It has been shown in clinical and preclinical studies that heightened consumption of fish oil containing x-3 polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), can reduce disease symptoms in lupus and RA patients (Pestka 2010;Arriens et al. 2015;Akbar et al. 2017;Li et al. 2019;Djuricic and Calder 2021).These clinical findings are supported by preclinical studies demonstrating that dietary x-3 PUFAs suppress spontaneous autoimmunity development and extend life in several lupus-prone mouse models (Prickett et al. 1982;Robinson et al. 1985;Godfrey et al. 1986;Alexander et al. 1987;Watson et al. 1988;Westberg et al. 1989;Chandrasekar et al. 1995;Bhattacharya et al. 2003;Halade et al. 2013;Pestka et al. 2014).
Increasing x-3 PUFA intake might be an effective intervention against the triggering of autoimmunity by environmental agents such as cSiO 2 .In support of this contention, we have previously shown that female NZBWF1 mice exposed to cSiO 2 weekly from ages 8-to 11-weeks and fed DHA-amended diets at a human equivalent dose (HED) of 2 to 5 g/d DHA exhibited a dramatic reduction in autoimmunity with significantly less inflammation, ELT, AAb, and glomerulonephritis compared to cSiO 2 -exposed mice fed non-DHA diets (Bates et al. 2016;Benninghoff et al. 2019;Gilley et al. 2020;Rajasinghe et al. 2020;Pestka et al. 2021).
A limitation of these investigations of cSiO 2 -induced lupus and x-3 intervention to date was the exclusive employment of young adult mice (8-11 weeks of age) for cSiO 2 exposures.This age range precedes the spontaneous onset of immunological tolerance breach, which could influence cSiO 2 -induced autoimmune response.Furthermore, this life-stage corresponds to a human age of 12 to 20 yr (Flurkey et al. 2007;Hagan 2017), which is an important consideration because occupational exposures to cSiO 2 predominantly begin in adult workers during their third decade of life (Ranaan et al 2022).In this investigation, we addressed these limitations by employing mature adult mice (16-19 weeks of age)-a life-stage documented to coincide with the onset of immunological tolerance breach (Richard and Gilkeson 2018) and one that more appropriately represents the age (20-30 yr) of cSiO 2 -exposed workers (Flurkey et al. 2007;Hagan 2017).The goals of this study were twofold.The first was to characterize how mature adult female NZBWF1 lupus-prone mice responded to subchronic cSiO 2 exposure.The second was to determine how DHA supplementation altered cSiO 2 -induced inflammation and autoimmunity in this mature adult disease model.

Animals and diets
All experiments involving mice were sanctioned by the Institutional Animal Care and Use Committee (IACUC) of Michigan State University, under approval number AUF # 201800113.We acquired mature adult female NZBWF1 mice, 14-weeks old, from Jackson Laboratories (Bar Harbor, ME).The mice were housed in groups of three per cage and provided with continuous access to food and water throughout the experiment.The animal facility maintained a stable environment, with a consistent temperature range of 21-24 � C, humidity maintained between 40-55%, and a regular 12-h light/dark cycle.
The Control (CON) diet based on the American Institute of Nutrition (AIN)-93G guidelines (Reeves et al. 2009) and a DHA-enriched variant were prepared following previously described methods (Bates et al. 2016) (Table 1).The CON diet comprised 70 g/kg of fat, including 10 g/kg of foodgrade corn oil and 60 g/kg of high-oleic safflower oil (LouAna, Brea, CA, USA), ensuring adequate essential fatty acids.In the DHA-supplemented diet, 25 g/kg of safflower oil was substituted with 25 g/kg of microalgal oil containing 40% DHA (DHASCO; DSM Nutritional Products, Columbia, MD, USA).This alteration resulted in a diet containing 10 g/kg of DHA, calorically equivalent to a human equivalent dose (HED) of 5 g/d (Bates et al. 2018).Diets were freshly prepared on a weekly basis to mitigate fatty acid oxidation and were stored at −20 � C. The mice received a daily supply of fresh food.

Experimental design
Figure 1 depicts the overall experimental design.Specifically, we tested two hypotheses in the female NZBWF1 mouse model: (i) mature adult mice receiving the same instilled dose of cSiO 2 used in prior studies would develop similar or more severe pulmonary and renal pathology compared to that previously observed in young mice, and (ii) DHAamended diet (HED 5 g/d) administered to cSiO 2 -exposed mature adult mice would attenuate the pulmonary autoimmune response and glomerulonephritis like that previously observed in young mice.Briefly, 14-week-old female NZBWF1 mice were randomly sorted into experimental groups and fed either CON or DHA-supplemented diets.At 16 weeks of age, mice were intranasally instilled once weekly for four consecutive weeks with either 25 mL PBS vehicle (VEH) or 1.0 mg of cSiO 2 (Min-U-Sil-5, 1.5-2.0mm average particle size, Pennsylvania Sand Glass Corporation, Pittsburgh, PA, USA) suspended in PBS.Therefore, there were a total of three experimental groups as follows: (i) VEH-treated mice fed CON diet (VEH/CON, n ¼ 12), (ii) cSiO 2 -instilled mice administered CON diet (cSiO 2 /CON, n ¼ 12), and (iii) cSiO 2 -instilled mice administered DHAsupplemented diet (cSiO 2 /DHA, n ¼ 12).For instillations, mice were anesthetized with 4% isoflurane.Following the final cSiO 2 instillation (19 weeks of age), urine was collected weekly until the time of necropsy and evaluated for proteinuria (�300 mg/dL protein) using diagnostic dipsticks (Cortez Diagnostics, Calabasas, CA, USA).One cohort of mice (n ¼ 6) from each experimental group was sacrificed one-week postinstillation last cSiO 2 instillation (PI), while the remaining mice were sacrificed at 5 weeks PI.

Animal necropsy, lung lavage, and tissue selection
Prior to exsanguination via the abdominal aorta, mice were rendered unconscious through intraperitoneal administration of sodium pentobarbital (56 mg/kg).The abdominal vena cava was accessed to collect blood for subsequent analysis of plasma and red blood cells, following the procedure outlined below.Subsequent to the cessation of vital signs, the trachea was exposed and cannulated, and the heart and lungs were carefully removed as a unit from the carcass.A saline solution of 0.8 mL was introduced into the lung via intratracheal instillation and then withdrawn to recover bronchoalveolar lavage fluid (BALF).This process was repeated with a second saline lavage, and the resulting BALF was pooled with the initial sample for further analysis.The left lung lobe was inflation fixed via the trachea with 10% neutral buffered formalin (NBF) (Fischer Scientific, Pittsburgh, PA, USA) at a constant pressure of 30 cm H 2 O for a minimum of 1 hr.One whole kidney was also fixed in 10% NBF.Tissues fixed in NBF remained fixative for 72 hr before being processed for histology.Right cranial lung lobes were frozen at −80 C for chemokine analysis.In addition, the caudal lung lobe was stored in RNA later at (Thermo Fisher Scientific, Wilmington, DE, USA) at 4 � C for 16 hr, then moved to −80 � C. Collected venous blood was spun down by centrifuge at 3500 � g for 10 min at 4 � C to separate the plasma and RBCs.Both plasma and RBCs were stored at −80 � C for AAb microarray and fatty acid analysis.

BALF cytology
A hemocytometer was used to determine total BALF leukocyte cell counts.Centrifugal forces (40 � g for 10 min) were used to immobilize BALF cells onto glass slides (Shandon Cytospin 3) that were then air-dried and stained with Diff-Quick (Fisher Scientific).Unused BALF was centrifuged at 465 � g for 15 min, and the resultant supernatant fraction was stored at −80 � C. BALF differential inflammatory cell counts were performed based on morphological criteria to identify monocytes, neutrophils, lymphocytes, and eosinophils out of 200 cells per cytological slide.

Fatty acid analysis
The analysis of fatty acid composition in red blood cells (RBCs) was conducted through gas-liquid chromatography by OmegaQuant Analytics, LLC (Sioux Falls, SD).The RBC Omega-3 Index, recognized as a biomarker for tissue x-3 content (Wierenga et al. 2020), was calculated by summing the percentages of EPA and DHA (%EPA þ DHA) in relation to the total fatty acids.

Lung and kidney histopathology
The left lung lobes, fixed in formalin, were cut into approximately 2 mm thick transverse tissue blocks, totaling four blocks each.These blocks were then embedded in paraffin.Tissue sections of 5 mm thickness were prepared and subjected to deparaffinization, followed by staining with hematoxylin and eosin (H&E) for subsequent microscopic evaluation of histopathology.Unbiased semi-quantitative analyses were conducted by a board-certified veterinary pathologist (JRH) with expertise in mouse lung histopathology using a well-defined set of histopathologic criteria and without previous knowledge of individual animal exposure/ treatment history ('blinded').Lung lesions scored for severity encompassed (a) perivascular and peribronchiolar ectopic lymphoid tissue (ELT), (b) alveolar proteinosis, (c) alveolitis, (d) alveolar type II epithelial cell hyperplasia and (e) mucous cell metaplasia in the bronchiolar epithelium.This overall approach kept intact the spatial aspects of these lymphoid interstitial cell/tissue lesions in the lung, which are lost with other analytical techniques like flow cytometry.
Similarly, the fixed kidney tissues were sectioned into three transverse sections and prepared for histopathological analysis.These sections were stained using Periodic acid-Schiff and hematoxylin (PASH) stain or subjected to immunohistochemical staining with IgG.The board-certified veterinary pathologist assessed the kidney tissue, evaluating tubular proteinosis, global proliferative and sclerosing glomerulonephritis using a modified International Society of Nephrology/Renal Pathology Lupus Nephritis Classification system (Weening et al. 2004), and glomerular IgG deposition.

Immunohistochemistry and morphometric analysis of lung and kidney
Immunohistochemistry (IHC) was carried out on lung and kidney tissue sections that had been formalin-fixed and embedded in paraffin using antibodies and employed in previous studies of cSiO2 in lupus-prone mice (Bates et al. 2015(Bates et al. , 2016(Bates et al. , 2018;;Heine et al. 2022).Specifically, in the lung tissue, B-cells were identified using a 1:600 dilution of rat anti-CD45R monoclonal antibody, while T-cells were identified using a 1:250 dilution of rabbit anti-CD3 polyclonal antibody (refer to Supplementary Table 1).Additionally, IgG þ plasma cells in the lung and IgG deposition in the kidney were identified using a goat anti-IgG polyclonal antibody (refer to Supplementary Table 1).
The slides were scanned using the VS200 virtual slide system (Olympus, Hicksville, NY, USA) utilizing NewCast software (Visiopharm, Hoersholm, Denmark).Each glass slide was randomly subsampled to acquire a minimum of 100 digital microscopic images from lung tissue sections captured at 20X magnification.We utilized mathematically robust, well-proven/documented methods of image analysis and stereological assessment of digital images (STEPanizer 1.8 Stereology Tool (Tschanz et al. 2011)) to ensure unbiased digital image sampling and morphometric/stereological analysis.Briefly, pulmonary CD45R þ , CD3 þ , and IgG þ lymphoid cells were enumerated by superimposing a point grid on the randomly sampled images.Total cell densities were computed by dividing the grid points overlaying positively stained cells by the reference tissue area, yielding a percentage of the overall lung area exhibiting positive staining.

Multiplex analysis of cytokines
Right cranial lung lobes were weighed and placed in appropriate amounts of 1X RIPA buffer (Thermo Fisher Scientific) and protease inhibitor (1:100 dilution; Thermo Fisher Scientific) to make 20% homogenates.Lung tissue was homogenized using the Qiagen TissueLyser bead mill, and protein concentrations were measured by BCA assay (Thermo Fisher Scientific).Protein concentrations were then normalized to 1000 ug/mL/sample.All samples were submitted to Eve Technologies Corp. (Calgary, Alberta) for quantification of chemokines using a multiplexed array that contained the target analytes MCP-5, IP-10, MIP-1a, MIP-1b, MIP-3b, MIG, MCP-1, and KC(MilliporeSigma, Burlington, Massachusetts, USA).Assay sensitivities for these chemokines ranged from 0.3 − 30.6 pg/mL.

NanoString autoimmune gene profiling
Lung tissue RNA (collected at 1-and 5-weeks post-infection) was extracted using RNeasy Mini Kits with DNase treatment (Qiagen, Valencia, CA).Subsequently, the extracted RNA was dissolved in nuclease-free water, its quantity determined using Qubit (Thermo Fisher Scientific), and its integrity validated using a TapeStation (Agilent Technologies).Samples with RNA integrity exceeding 8 were subjected to analysis using the NanoString Autoimmune Gene Expression assay (XT-CSO-MAIP1-12, NanoString Technologies, Seattle, WA, USA) at the MSU Genomics Core.The assays were conducted and reparated on the nCounter MAX system, sample reparationn station, and digital analyzer (NanoString Technologies), following the provided manufacturer's guidelines.
We evaluated the influence of cSiO 2 exposure and experimental diets on annotated gene sets by computing both global and directed significance scores for each pathway at various time points, following the approach outlined in a previous study (Benninghoff et al. 2020).The global score provides an estimation of cumulative evidence regarding differential gene expression within a pathway, serving as a complementary tool to compare pathways and distinguish between experimental groups.Pathway Z scores were computed as the Z-scaled first principal component of the normalized expression of genes associated with the pathway.
For unsupervised hierarchical cluster analyses (HCC) using log2 transcript count data for Differentially Expressed Genes (DEGs), ClustVis (Metsalu, 2015, #33) was employed.Additionally, Spearman rank correlations were conducted to investigate overall patterns in gene expression profiles by comparing the pathway Z score with other disease biomarkers in lung tissues at 1-and 5-weeks postinfection (PI).A significant correlation was considered when the Spearman correlation coefficient (q) was more significant than 0.5 or less than −0.5, and the p-value was less than 0.05.

AAb microarray
BALF and plasma samples underwent assessment for the presence of AAbs through GeneCopoeia (Rockville, Maryland).Diluted plasma and BALF samples were incubated on microscope slides, each containing 16 identical OmicsArray TM panels consisting of 120 known autoantigens affixed to nitrocellulose filters.One panel was designated for a PBS-negative sample control.After incubation, the slides underwent washing and subsequent incubation with Cy3labeled anti-mouse IgG and Cy5-labeled anti-mouse IgM secondary antibodies.Following this, the slides were rewashed, and fluorescent signals (532 nm for Cy3/IgG, 635 nm for Cy5/IgM) were detected using a GenePix TM 4000B two-channel laser microarray scanner (Molecular Devices, San Jose, California).The fluorescent signal intensities were determined using GenePixPro TM 7.0 software (Molecular Devices, San Jose, California).To calculate antibody scores, normalized signal intensities (NSI) and signal-to-noise ratios (SNR) were utilized in the following equation: The Ab-score values, normalized and scaled for unit variance, were presented visually using ClustVis software (Metsalu, 2015).The data were subjected to clustering using unsupervised hierarchical co-clustering (HCC).The rows were clustered using Euclidean distance and Ward linkage, with imputation applied for estimating missing values.Selected AAb-score values were presented as violin plots, generated using Prism 9 (GraphPad Prism v 9.2, San Diego, CA, USA).

Statistical analysis
All data underwent analysis and subsequent statistical tests utilizing Prism 9 (GraphPad Prism v 9.2, San Diego, CA, USA), with the exclusion of the NanoString gene expression data, as previously mentioned.Examination for outliers was conducted utilizing Grubb's outlier test (with Q ¼ 1%), and normality was assessed using the Shapiro-Wilk test (p < 0.01).Regarding the histopathological endpoints, a oneway ANOVA was employed to explore our hypothesis that dietary DHA would mitigate cSiO2-triggered responses at each time point.For non-normal and semi-quantitative data, analysis was performed using the nonparametric Kruskal-Wallis test.The presentation of data includes mean ± standard error of the mean (SEM), and a p-value of � 0.05 was regarded as statistically significant.

DHA consumption increases omega-3 index
cSiO 2 exposure and experimental diets did not influence body weight increases (Supplementary Figure 1).Consistent with previous studies (Bates et al. 2016;Gilley et al. 2020), the replacement of high-oleic sunflower oil with DHAenriched algal oil in cSiO 2 /DHA diets corresponded with increased x-3 PUFA content in RBCs from mice necropsied at both 1 and 5 weeks PI (Table 2; Figure 2).Concomitantly, cSiO 2 /DHA mice had significantly reduced percentage of total omega-6 PUFAs in their RBCs compared to cSiO 2 /CON-fed mice.DHA content in RBCs was significantly increased in DHA-fed mice compared to those on the CON diet at the expense of arachidonic acid.Moreover, the omega-3 index, which is a biomarker that describes the sum of the DHA þ EPA in RBCs, was three times higher in cSiO 2 /DHA mice (17-19%) compared to either VEH/CON and cSiO 2 /CON mice (5-6%).

DHA attenuates cSiO 2 -inducedpulmonary inflammatory cell infiltration and ELT formation
The most conspicuous lung lesions in mice instilled with cSiO 2 and fed CON diet were lymphoplasmacytic infiltrations in perivascular and peribronchiolar interstitial (ectopic lymphoid tissue, ELT) (Figure 3A).cSiO 2 -instilled mice also had mild to moderate mixed inflammatory cell infiltrates in the alveolar parenchyma (alveolitis; composed of neutrophils, lymphocytes, and monocytes) that were associated with accumulations of extracellular proteinaceous material (proteinosis) and cSiO 2 particles (extracellular and intracellular) throughout the alveolar airspaces.cSiO 2 particle-laden alveolar macrophages with morphologic features of degeneration (vacuolation) and necrosis (cellular enlargement, cytoplasmic vacuolation, and nuclear pyknosis or karyorrhexis) was another frequent finding in these cSiO 2 -instilled lungs at both 1-and 5-weeks PI.Lungs of mice at 5 weeks, but not 1-week PI, also had widely scattered small granulomas, composed of macrophages, lymphocytes, and cSiO 2 particles, in alveolar septa.
ELT onset was most severe in the lungs of mice 5 weeks after the last cSiO 2 instillation (Figure 3(A)), with significantly lesser amounts in mice 1 week PI.At both time points, no or minimal perivascular/bronchiolar lymphoid cell infiltrates were present in VEH/CON mice.VEH/CON mice also lacked other lung histopathology (e.g.alveolitis).In contrast to the large amounts of ELT in cSiO 2 /CON mice, cSiO 2 -exposed DHA-fed mice had only small amounts of ELT in their lungs at 1 and 5 weeks PI.Dietary DHA supplementation, however, had minimal to no apparent attenuation of other cSiO 2 -triggered pulmonary pathology, including alveolar accumulation of amorphous protein, silica, macrophages, and inflammatory cells.Semi-quantitative severity scores of lung lesions supported histopathological observations that cSiO 2 exposure significantly increased inflammation, ELT formation, and alveolar proteinosis at both timepoints (Figure 3(B)).Similarly, dietary supplementation with DHA markedly attenuated ELT formation but did not affect alveolitis and alveolar proteinosis.cSiO 2 exposure significantly elevated total leukocytes, monocytes, and neutrophils at 1 and 5 weeks PI in the BALF compared to VEH/CON mice.In contrast, DHA consumption reduced the numbers of total leukocytes and monocytes in the BALF but interestingly resulted in increased neutrophil numbers at 5 weeks PI compared to cSiO 2 /CON mice (Figure 3(C)).

DHA supplementation suppresses cSiO 2 -induced B-, T-, and plasma cell accumulation in lung
Lymphoid cell populations in ELT were identified using IHC (Figure 5(A)) and quantified using standard morphometric techniques (Figure 5(B)).cSiO 2 -induced ELT was composed predominantly of CD3 þ T-cells and CD45R þ Bcells, with smaller numbers of IgG þ plasma cells located in the margins of ELT.cSiO 2 -triggered lymphoid cell infiltration was significant at 1 week PI and markedly increased at 5-weeks PI.Dietary supplementation with DHA significantly reduced CD45R þ B-cell, CD3 þ T-cell, and IgG þ plasma cell infiltration at 5-weeks PI.

DHA quells cSiO 2 -triggered autoimmune-related gene expression in lung
NanoString global and directed significance scores revealed that immunological pathways were significantly altered in lung tissue by both cSiO 2 exposure and diet (Figure 6).cSiO 2 exposure led to the activation of autoimmune-related pathways such as MHC Class I antigen presentation, Nodlike receptor (NLR) signaling, and Type I/II IFN signaling at 1 and 5 weeks PI.DHA-fed cSiO 2 -exposed mice had significantly reduced enrichment of these pathways at 1 week PI.cSiO 2 exposure resulted in the upregulation of genes associated with several significantly enriched pathways at 1 and 5 weeks PI compared to VEH/CON mice (Supplementary Figures 2 and 3).DHA significantly downregulated representative genes associated with NLR signaling (e.g.Cyld, Tlr4, Casp4) (Supplementary Figure 2 A), MHC Class I antigen presentation (e.g.Fcgr1, Herc6) (Supplementary Figure 2B,D), cytosolic DNA sensing (e.g.Ddx58, Zbp1) (Supplementary Figure 2C,D), and Type I/II IFN signaling (e.g.Irf7, Mx1, Isg15) (Supplementary Figure 3 A-C) at 1-week PI.

DHA suppresses diverse IgG AAb responses in BALF induced by cSiO 2
AAg microarray revealed that cSiO 2 induced a diverse range ofIgG AAbs (Figure 7) in the BALF of CON-fed that was less.These responses were markedly less robust in DHA-fed mice.cSiO 2 also induced IgMAAbs in CON-fed mice, but these responses were not affected by DHA (Supplementary Figure 4).Significant differences were not detected among VEH/CON, cSiO 2 /CON, and cSiO 2 /DHA groups regarding IgG AAbs in the plasma (Supplementary Figure 5).

DHA suppresses cSiO 2 -induced hypertrophy and IgG deposition in the kidney
Mild histopathological changes were observed in renal tissuefromcSiO 2 /CONmice at 5-weeks PI (Figure 8).These In VEH/CON and cSiO 2 /CON mice, total omega-6 fatty acids were more abundant than x-3 fatty acids in RBC cell membranes at 1week and 5-weeks PI.In cSiO 2 /DHA mice, total omega-6 fatty acids were significantly reduced, whereas total x-3 fatty acids were significantly increased at both time points.Letters: a, significantly different from VEH/CON for the specified endpoint (p < 0.05); b, significantly different from cSiO 2 /CON for the specified endpoint (p < 0.05).4) marked (51-75%); and ( 5) severe (>75%) amount of tissue affected.(C) cSiO 2 instillation caused a significant increase in total leukocytes, monocytes, and neutrophils in the BALF.DHA significantly reduced total leukocyte and monocyte cellularity in the BALF but did not influence neutrophils.a, significantly different from VEH/CON group; b, significantly different from cSiO 2 /CON group; p < 0.05.mice had modest glomerular hypertrophy (Figure 8(B)) and mild renal IgG deposition (Figure 8(E)) compared to VEH/ CON mice (Figure 8(A,D)).Renal pathology and IgG immunohistochemical staining were absent in cSiO 2 /DHA mice (Figure 8(C,F)).Consistent with histopathological analysis, semi-quantitative severity scoring revealed mild but significant increases in glomerular hypertrophy and renal IgG deposition at 5-weeks PI (Figure 8(G-H)).These cSiO 2induced glomerular changes were attenuated in DHA-fed mice.Concordant with the mild nature of glomerular inflammation, urinalysis revealed no evidence of proteinuria in mice exposed to cSiO 2 (Supplementary Figure 6).

Discussion
Researchers often categorize preclinical mouse models into specific age groups to understand how human developmental stages influence disease pathogenesis (Flurkey et al. 2007;Hagan 2017).Radulescu and colleagues (Radulescu et al. 2021) have recently introduced a set of life-stage categories, which included juvenile (< 4 weeks), young adult (4-16 weeks), mature adult (16-70 weeks), and late adult (70-98 weeks).Since these life stages represent different human ages and phases of immunological tolerance breach, they are potentially helpful reference points for investigating toxicant-triggered disease modulation in preclinical lupus models.Here, we queried for the first time how cSiO 2 treatment and DHA intervention impact early inflammatory and autoimmune responses in the lungs of mature adult female NZBWF1 mice that appropriately represent 20 to 30-yearold individuals occupationally exposed to cSiO 2 (Raanan et al. 2022).
We have previously established that ELT neogenesis in the lung serves as a critical nexus for cSiO 2 -induced autoimmunity and a target for DHA intervention in the NZBWF1 mouse (Bates et al. 2016(Bates et al. , 2018(Bates et al. , 2019;;Benninghoff et al. 2019;Wierenga et al. 2019Wierenga et al. , 2020)).When ELT morphometric data from this study were compared to that previously reported in young adult mice (Bates et al. 2019) (Figure 9), we found that (i) cSiO 2 exposure induced significant infiltration of CD3 þ T-cells at 5-weeks PI in mature adult mice but not in young adult mice, (ii) cSiO 2 -induced infiltration of CD45R þ B-cells at 5-weeks PI in mature adult mice was greater than that in young adult mice, and (iii) DHA significantly reduced CD3 þ T-cell infiltration in mature adult cSiO 2 -exposed mice and CD45R þ B-cell infiltration in both mature adult and young adult mice.
NanoString analysis of global and targeted significance scores revealed insights into potential mechanisms for profound histopathologic and immunologic changes induced by cSiO 2 within lung tissue.Notably, there were notable alterations of autoimmune-related pathways including MHC Class I antigen presentation, NLR signaling, and Type I/II IFN signaling as early as 1-week PI.In prior studies using young mice, these effects were essentially discernable at 5weeks PI (Benninghoff et al. 2019).Importantly, when DHA-fed mice were exposed to cSiO 2 , a substantial reduction in the enrichment of these autoimmune-related  pathways was observed at 1-week PI.This finding implies that the inclusion of DHA in the diet may have a mitigating effect at the transcriptional level on the activation of immune pathways induced by cSiO 2 exposure.The observed reduction in pathway enrichment here and previously highlights the intricate interplay between environmental exposures and dietary influences on immunological processes in the lung tissue.
Interestingly, while the cSiO 2 -triggered endpoints in the lungs of mature adult mice were relatively robust, we observed only mild pathology in the kidney at 5-weeks PI.These changes in renal pathology are consistent with minimally elevated IgG AAb responses in the plasma of cSiO 2 / CON mice.In previous studies focused on cSiO 2 -triggered glomerulonephritis in young NZBWF1 mice, proteinuria was not evident until at approximately 10-weeks after cSiO 2 -exposure with concomitant renal pathology was observed at 12-weeks PI (Bates et al. 2015).Considering the lung serves as the nexus for respirable cSiO 2 -mediated autoimmune responses in this model, likely, the threshold for recurring inflammation originating from the lung necessary for robust downstream damage to the kidney was not reached at 5-weeks PI in this experimental model.It is possible that if cSiO 2 -exposed mature adult mice were euthanized later (e.g.9-weeks PI), we might observe more advanced glomerulonephritis with proteinuria.
Figure 10 qualitatively compares the severity of cSiO 2triggered inflammatory/autoimmune endpoints and effects of DHA supplementation in young adult female NZBWF1 mice at 5-weeks PI as reported in prior studies (Bates et al. 2015(Bates et al. , 2016(Bates et al. , 2019;;Benninghoff et al. 2019;Gilley et al. 2020;Rajasinghe et al. 2020;Pestka et al. 2021) and mature adult NZBWF1 mice at 5-weeks PI as determined in the present investigation.Weekly exposure of young adult mice to 1 mg cSiO 2 for 4 weeks led to modest pathological changes and ELT development in the lung and no observable glomerulonephritis.In contrast, exposure of mature adult mice to the same cSiO 2 treatment resulted in marked pulmonary pathology and with mild but significant glomerular hypertrophy and IgG deposition in the kidney consistent with glomerulonephritis demonstrating the age-dependent response to cSiO 2 in this experimental model.Accordingly, our data suggest that mouse age influences cSiO 2 -triggered inflammatory and autoimmune responses but not preventive effects of DHA.
Currently, the most common drugs used for treating lupus are glucocorticoids (e.g.prednisone), antimalarials (e.g.hydroxychloroquine), immunosuppressants (e.g.cyclophosphamide), and monoclonal antibodies (Basta et al. 2020).Although treatment with these drugs effectively manages and slows disease progression, their chronic use is associated with significant adverse effects (Flammer and Rogatsky 2011;Porta et al. 2020).While newer biologic therapies have yet to demonstrate similar off-target toxic effects, they are expensive and remain unaffordable for many lupus patients (Lamore et al. 2012).Therefore, there is a critical need for nontoxic, cost-effective therapies to protect against environmental-triggered lupus.Toward that end, DHA was efficacious in attenuating cSiO 2 -induced inflammation, ELT formation, Type I/II IFN, innate and adaptive immune response gene signatures, and IgG AAb production in the lung at both 1-and 5-weeks PI with no evident toxicity.These findings and those in previous studies (Bates et al. 2016(Bates et al. , 2018;;Benninghoff et al. 2019;Gilley et al. 2020;Rajasinghe et al. 2020) collectively support the notion that x-3 PUFA supplementation is a potential steroid-sparing approach to prevent/treat lupus onset and flaring triggered by environmental agents like airborne cSiO 2.
There are multiple mechanisms by which x-3s can interfere with the cSiO 2 -induced autoimmune phenotype.x-3 PUFAs possess the capability to inhibit the metabolism of x-6 PUFAs into proinflammatory eicosanoids, including thromboxanes, prostaglandins, and leukotrienes (Lands et al. 2018).The lipid byproducts from the arachidonic acid cascade predominantly exhibit inflammatory actions, especially during episodes of acute inflammation.Shifting the PUFA balance in favor of x-3 PUFAs, as opposed to x-6 PUFAs like arachidonic acid, has the potential to augment the proresolving characteristics promoted by lipid mediators derived from x-3s.Recent research has demonstrated a robust correlation between x-3 PUFA levels in both plasma and red blood cells and the subsequent production of downstream lipid mediators (Ostermann and Schebb 2017).Importantly, supplementation with DHA or EPA led to a reduction in x-6 PUFAs, particularly arachidonic acid, along with a decrease in x-6 PUFA-derived metabolites.In line with this mechanism, we have recently illustrated that DHA supplementation effectively suppresses the induction of various cSiO 2 -induced eicosanoids in alveolar macrophages (Favor et al. 2023).
In addition to their competition with x-6 PUFAs, x-3 PUFAs can directly impact inflammatory pathways (Calder 2017).For instance, DHA and EPA disrupt the activation of transmembrane receptors associated with inflammatory signaling by enhancing membrane fluidity and inhibiting the formation of lipid rafts (Wong et al. 2009).Additionally, both extracellular and intracellular phospholipases can cleave PUFAs from the cell membrane, and the resulting liberated DHA and EPA may stimulate transmembrane receptors or intracellular receptors known for their role in suppressing proinflammatory signaling (Li et al. 2013;Yan et al. 2013).Specifically, x-3 PUFAs can counteract Toll-like receptor (TLR) activation (Weatherill et al. 2005;Hwang et al. 2016) and interfere with NF-kB-dependent transcription by activating peroxisome proliferator-activated receptor gamma (PPARc) (Ricote and Glass 2007;Chang et al. 2015).Consistent with this mechanism, we have demonstrated that DHA suppresses cSiO 2 -induced inflammasome activation and IL-1 cytokine release in macrophages by acting at the level of TLR-mediated priming.Furthermore, both DHA and EPA undergo metabolism to generate specialized pro-resolving mediators (SPMs) like maresins, resolvins, protectins, and anti-inflammatory epoxide metabolites (Serhan et al. 2015;Ostermann and Schebb 2017).These SPMs effectively inhibit inflammatory signaling (Titos et al. 2016;Sham et al. 2018) and facilitate the process of efferocytosis, which is crucial for the removal of deceased cells, a critical step in thwarting the pathogenesis of autoimmune diseases (Chiang et al. 2015;Fredman et al. 2016).
It should be noted that both here and previously (Bates et al. 2015(Bates et al. , 2016(Bates et al. , 2018;;Pestka et al. 2021), we chose to study the effects of cSiO 2 exclusively female NZBWF1 mice because these have higher lupus disease penetrance than male NZBWF1 mice (Burnet and Holmes 1965;Hicks and Burnet 1966), thus mimicking lupus predilection in human females (Tsokos 2011).However, we cannot exclude the possibility that males and females differentially respond to cSiO 2 relative to inflammation and autoimmunity.Consistent with this notion, an investigation of Chinese pottery workers reported that at the same average level of cSiO 2 exposure, males exhibited a higher risk of developing silicosis than females (Poinen-Rughooputh et al. 2021).Even more germane to the present study, Parks and coworkers (Parks et al. 2002) reported that the risk of lupus was higher among men than women occupationally exposed to cSiO 2 .From a preclinical perspective, following four weekly exposures to 1 mg cSiO 2 , male C57Bl6 mice, which are not lupus-prone, had worse alveolitis and greater dendritic cell presence within the lungs than their similarly treated female counterparts (Ray and Holian 2019).A later study in C57Bl6 mice employing a single 10 mg cSiO 2 dose reported a slight downregulation of classical fibrosis-related pathways in female mice compared with males; however, over the long term, there was no difference in the extent of fibrosis between the sexes (Jin et al. 2022).Finally, although in earlier studies by Holian and coworkers (Brown et al. 2003;2004), it was observed that both male and female NZM2410 mice were susceptible to cSiO 2 -accelerated lupus, unlike NZBWF1 mice, both biological sexes in this strain have a similar predilection for autoimmunity (Rudofsky and Lawrence 1999).Given the lack of clarity on effects of biological sex on cSiO2-induced inflammation and autoimmunity and the preponderance of males occupationally exposed to cSiO 2 , it will be desirable in future studies to compare the effects of this particle in male and female NZBWF1 mice.
This investigation had several other limitations.Firstly, we limited our endpoints to 1 and 5 weeks after the final cSiO 2 instillation, whereas our prior studies in young adult mice endpoints were determined in the lung at 1-, 5-, 9-, and 13-weeks PI.Subtle differences in autoimmune phenotype observed here between young and mature adult mice at the early time points might have been greatly magnified at 9-and 13-weeks PI, providing additional insight into the importance of the mature phenotype.Secondly, it would have been interesting to compare mature adult responses to cSiO 2 in control mice that were not lupus-prone.In prior studies, we found that subchronic exposure to cSiO 2 in young adult female C57Bl6 and NZB/LacJ, which are not lupus-prone, does not induce an autoimmune phenotype at 13 weeks PI (Bates et al. 2015(Bates et al. , 2016)).However, it cannot necessarily be assumed mature adult non-lupus mice would be similarly recalcitrant to autoimmune triggering by cSiO 2. Finally, it would have been informative to include mice fed  (Bates et al. 2015(Bates et al. , 2016(Bates et al. , 2019;;Benninghoff et al. 2019;Gilley et al. 2020;Rajasinghe et al. 2020;Pestka et al. 2021) and (B) mature adult NZBWF1mice at 5-weeks PI as determined in the present investigation.(A) Weekly exposure of young adult mice to 1 mg cSiO 2 for 4 weeks led to modest pathological changes and ELT development in the lung and no observable glomerulonephritis, whereas (B) exposure of mature adult mice to the same cSiO 2 resulted in marked pulmonary pathology and with significant glomerular hypertrophy and IgG deposition in kidney consistent with glomerulonephritis demonstrating the age-dependent response to cSiO 2 in this experimental model.
only DHA in our experimental design.In a prior study using female NZBWF1 mice, we compared the impact of feeding three different diets: a x-3 PUFA-rich diet with docosahexaenoic acid-enriched fish oil, an x-6 PUFA-rich diet with corn oil, and an x-9 monounsaturated fatty acid (MUFA)-rich diet (Pestka et al. 2014).Mice consuming the x-3 PUFA diet exhibited attenuated plasma autoantibodies, proteinuria, and glomerulonephritis at age 34-weeks compared to those on x-6 PUFA or x-9 MUFA diets.There was a generalized downregulation associated with inflammatory response, antigen presentation, T cell activation, B cell activation/differentiation, and leukocyte recruitment in mice fed the x-3 PUFA diet.Thus, the inclusion of a DHA-only group in future studies could provide additional understanding of the subtle effects of the x-3 PUFA diet alone on gene expression in lung and other autoimmune-related endpoints.

Conclusion
Subchronic IN administration of cSiO 2 to mature adult NZBW/F1 mice led to heightened pulmonary inflammatory and autoantibody responses, along with glomerulonephritis that were generally more pronounced at 5 weeks PI compared to previously documented effects in similarly treated young adult mice.Significantly, supplementation with DHA at a dosage relevant to human translation effectively alleviated cSiO 2 -induced inflammation and autoimmunity in mature adult mice, mirroring the protective outcomes observed in their younger counterparts.These results underscore the influence of life-stage as a contributing factor in this preclinical model and again emphasize the therapeutic potential of x-3 fatty acids in mitigating autoimmune responses triggered by toxicants.

Figure 2 .
Figure2.DHA supplementation skews long-chain PUFA profile in mature adult female NZBWF1 mice.In VEH/CON and cSiO 2 /CON mice, total omega-6 fatty acids were more abundant than x-3 fatty acids in RBC cell membranes at 1week and 5-weeks PI.In cSiO 2 /DHA mice, total omega-6 fatty acids were significantly reduced, whereas total x-3 fatty acids were significantly increased at both time points.Letters: a, significantly different from VEH/CON for the specified endpoint (p < 0.05); b, significantly different from cSiO 2 /CON for the specified endpoint (p < 0.05).

Figure 4 .
Figure 4. cSiO 2 -induced chemokine protein expression in lung tissue of mature adult female NZBWF1 mice is inhibited by DHA consumption.Proinflammatory cytokine production was measured in lung tissue homogenate using a multiplex cytokine discovery assay.Cytokine production significantly increased with cSiO 2 exposure and was attenuated with the DHA-enriched diets at either 1-week or 5-weeks PI.Letters: a, significantly different from VEH/CON for the specified endpoint (p < 0.05); b, significantly different from cSiO 2 /CON for the specified endpoint (p < 0.05).

Figure 5 .
Figure 5. cSiO 2 -induced lung infiltration of perivascular and peribronchiolar CD45R þ B-cells, CD3 þ T-cells, and IgG þ plasma cells in mature adult female NZBWF1 mice is suppressed by DHA feeding.(A-O) Light photomicrographs of lung tissue from VEH/CON, cSiO 2 /CON, and cSiO 2 /DHA mice at 5-weeks PI.Tissue sections were histochemically stained with hematoxylin and eosin (H&E; low power, A-C; high power, D-E) identifying perivascular and peribronchiolar ectopic lymphoid tissue (ELT, solid arrows in B and E) or scant lymphoid cell aggregates (stipple arrows in C).Other sections were immunohistochemically stained for CD45R þ B lymphoid cells (G-I), CD3 þ T-cells (J-L), and IgG þ plasma cells (M-O) in ELT.In cSiO 2 /CON mice, silica instillation triggered conspicuous formation of ELT containing CD45R þ B-cells, CD3 þ T-cells, and IgG þ plasma cells.Only scant amounts of lymphoid cells were present in the lungs of cSiO 2 /DHA mice (C, F, I, L,O).DHA treatment markedly attenuated lymphoid cell infiltration.b, bronchioles; v, blood vessels; a, alveolar parenchyma.(P) Graphical representation of morphometrically determined density of CD45R þ B-cells, CD3 þ T-cells, and IgG þ plasma cells in lung tissue of mice at 1 week and 5 weeks after instillation.a, significantly different from VEH/CON group; b, significantly different from cSiO 2 /CON group; p < 0.05.

Figure 6 .
Figure 6.SiO 2 -induced inflammatory/autoimmune pathways in lung tissue 1-and 5-weeks post-cSiO 2 instillation in mature adult female NZBWF1 mice are quelled by DHA supplementation.Z scores of selected inflammatory/autoimmune pathways are presented as Tukey box plots for select pathways of interest for lung tissue.Within a tissue type, different letters indicate that the treatment groups are significantly different (p < 0.05), as described in materials and methods.cSiO 2 exposure significantly enriched MHC antigen presentation, NLR signaling, and Type I/II Interferon signaling pathways.DHA significantly reduced pathway Z scores for MHC antigen presentation and Type I/II Interferon signaling at 1-week PI and for NLR signaling at both 1-and 5-weeks PI.Letters: a, significantly different from VEH/ CON for the specified endpoint (p < 0.05); b, significantly different from cSiO 2 /CON for the specified endpoint (p < 0.05).

Figure 7 .
Figure 7. DHA feeding affects cSiO 2 -induced IgG AAb responses in the BALF at 5-weeks PI in mature adult female NZBWF1 mice.(A) AAb-score values at 5 weeks PI in BALF for the expression of 120 IgG AAbs are illustrated in a heatmap using unsupervised clustering (Euclidian distance method).Scale bar values reflect the range of variance stabilized AAb scores centered across rows.(B) cSiO 2 induced increase total IgG AAb the BALF in mice-fed control diet but not in mice fed DHA.(C) cSiO 2 exposure significantly increased various classes of autoimmunity-related AAbs detected in the BALF.A downward trend was observed in the same classes of cSiO 2 -triggered AAbs when mice were fed DHA.Letter a indicates significantly different from VEH/CON for the specified endpoint (p < 0.05).

Figure 8 .
Figure 8. cSiO 2 -triggered membranoproliferative glomerulonephritis and glomerular IgG deposition at 5-weeks PI in mature adult female NZBWF1 mice is attenuated by DHA feeding.Light photomicrographs of kidney tissue stained with periodic acid Schiff (PAS) and hematoxylin (A, B, C) and immunohistochemically stained for IgG with hematoxylin counterstain (D, E, F).VEH/CON (A, D), cSiO 2 /CON (B, E), and cSiO 2 /DHA mice (C, F). cSiO 2 -exposed mice (B, E) had modest increases in glomerular size, cellularity, size, and IgG deposition.No histopathology was observed in VEH/CON mice (A, D) and DHA-fed mice (C, F).Individual kidney sections were semi-quantitatively scored based on the modified International Society of nephrology/renal pathology lupus Nephritis Classification system described methods for lupus nephritis score for hyperplasia/hypertrophy (G) and IgG deposition (H).cSiO 2 /CON mice had significantly increased severity scores for hyperplasia/ hypertrophy and IgG deposition at 5-weeks PI.DHA-enriched diets attenuated renal hyperplasia and hypertrophy.G, glomeruli; arrow, IgG proteinaceous material.Letters: a, significantly different from VEH/CON for the specified endpoint (p < 0.05); b, significantly different from cSiO 2 /CON for the specified endpoint (p < 0.05).

Figure 9 .
Figure 9. cSiO2-triggered infiltration of T and B cells is greater in mature adult NZBWF1 mice compared to young adult NZBWF1 mice.Morphometric data from this study were compared to that previously reported in for young adult mice (Bates et al. 2019).(A) cSiO 2 exposure induced significant infiltration of CD3 þ T-cells at 5-wk PI in mature adult mice but not in young adult mice.(B) cSiO 2 -induced infiltration of CD45R þ B-cells at 5-weeks PI in mature adult mice was greater than that in young adult mice.DHA significantly reduced (A) CD3 þ T-cell infiltration in marine adult cSiO 2 -exposed mice and (B) CD45R þ B-cell infiltration in both mature adult and young adult mice.Letters: a, significantly different from VEH/CON for the specified endpoint (p < 0.05); b, significantly different from cSiO 2 /CON for the specified endpoint (p < 0.05); c, significantly different from young adult mice within the same treatment group and specified endpoint (p < 0.05).

Figure 10 .
Figure10.Mouse age influences cSiO 2 -triggered inflammatory and autoimmune responses but not preventive effects of DHA.Diagrammatical summary of the severity of cSiO 2 -triggered inflammatory/autoimmune endpoints and effects of DHA supplementation in (A) young adult female NZBWF1 mice at 5 weeks PI as reported in prior studies(Bates et al. 2015(Bates et al. , 2016(Bates et al.  , 2019;; Benninghoff et al. 2019;Gilley et al. 2020;Rajasinghe et al. 2020;Pestka et al. 2021) and (B) mature adult NZBWF1mice at 5-weeks PI as determined in the present investigation.(A) Weekly exposure of young adult mice to 1 mg cSiO 2 for 4 weeks led to modest pathological changes and ELT development in the lung and no observable glomerulonephritis, whereas (B) exposure of mature adult mice to the same cSiO 2 resulted in marked pulmonary pathology and with significant glomerular hypertrophy and IgG deposition in kidney consistent with glomerulonephritis demonstrating the age-dependent response to cSiO 2 in this experimental model.

Table 1 .
Formulation of experimental diets.
Figure1.Experimental design.Female NZBWF1 mice were obtained at 14 weeks of age and were initiated on either an AIN-93G (CON) diet or an isocaloric diet amended with 1% DHA (5 g/d human equivalent dose [HED]).At 16 weeks of age, mice were intranasally instilled with either saline (VEH) or 1 mg cSiO 2 1/week for four consecutive week.Proteinuria was assessed weekly starting at 20 weeks of age to monitor disease progression.Six animals per exposure/treatment group were sacrificed at 20 week of age (1-week PI), and the remaining 6 mice per group/treatment were euthanized at 24 week of age (5 weeks PI).At both timepoints, BALF was collected for differential cell counts and AAb microarray; blood was collected for AAb microarray and fatty acid analysis; lung and kidney tissues were collected for histopathology, immunohistochemistry (IHC), morphometric analysis, NanoString, and cytokine multiplex array.
†Analysis of fatty acid profiles of RBCs from experimental groups 1-and 5-weeks post-instillation as determined by OmegaQuant Analytics, LLC.p < 0.05; � Significant compared to VEH/CON, † Significant compared to cSiO 2 /CON.