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ABSTRACT
Matrix metalloproteinase-3 is invariably upregulated in cancerous condition. So we aimed to determine serum level of MMP-3 in canine mammary tumors. The gene was expressed in E. coli system as ~43kDa recombinant protein, which was refolded, purified, and confirmed. Hyperimmune serum was raised against the expressed protein in rabbits and mice to standardize sandwich ELISA. ROC analysis revealed largest area under the curve of 0.998 with sensitivity (100%) and specificity (95%) for a cut-off value of 0.363 with respect to histopathological staining. The finding of the present study indicates that MMP-3 can act as a potential molecular marker for serodiagnosis of canine mammary carcinomas.
Declaration of interest
The authors declare that they have no conflict of interest in relation to this study.
Funding
This research was funded by the Department of Biotechnology, Ministry of Science and Technology (Grant ID: BT/PR6434/GBD/27/414/2012) and by the Department of Science and Technology, Ministry of Science and Technology (Grant ID: DST/INSPIRE FELLOWSHIP/2013/145).