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Lily symptomless virus (LSV) is the most common lily virus, being detected in many species and hybrids. We established a microsphere-based fluorescent immunoassay for the determination of LSV, using a polyclonal antibody against LSV covalently bound to carboxy-modified microspheres able to capture LSV antigen. A monoclonal antibody against LSV conjugated to quantum dots (QDs) was used as a fluorescent probe, enabling LSV to be fluorescently detected by a combination of encoded beads and QDs. This method was 16 times more sensitive than ELISA in the detection of LSV, and could potentially be applied to the simultaneous detection of inhomogeneous matter.
ACKNOWLEDGMENTS
This work was financially supported by the National Natural Science Foundation of China (NSFC) (No. 20775076 and No. 20875084) and a Strategic Eleventh-Five-Year Science and Technology Supporting Grant (No. 2006BAK10B09) from the Ministry of National Science and Technology of China.