Phytochemical study on the aerial parts of Anemone taipaiensis for the first time led to the isolation of two new oleanane-type triterpenoid saponins 1 and 2, together with four known saponins (3–6). Their structures were elucidated by extensive spectroscopic analysis and chemical evidences. Saponins 2–4 exhibited cytotoxicity against human glioblastoma U251MG cell line with IC₅₀ values ranging from 1.56 to 80.62 μM.