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Articles

Modified coir pith with glucose syrup as a supporter in non-external nutrient supplied biofilter for benzene removal by Bacillus megaterium

, , , &
Pages 3607-3618
Received 02 Oct 2018
Accepted 01 May 2019
Accepted author version posted online: 12 May 2019
Published online: 16 May 2019

ABSTRACT

Coir pith glucose syrup beads were used as a supporter in a biofilter system. The modified coir pith beads provided a carbon source and controlled humidity for microorganism growth for long-term operation without external nutrient supplementation. For the screening, Bacillus spp. were immobilised on coir pith beads and used for benzene bioremediation. The result showed that coir pith beads immobilised with Bacillus megaterium can remove on average 85–100% of the benzene (215-day operation). In addition, B. megaterium presented the ability to transform benzene to catechol. For an up-scaled application, a 25-L biofilter system was developed and tested in a closed 24-m3 container re-injected with 0.6 ppm benzene for 8 cycles. The system presented the ability to remove 100% of the benzene. This biofilter has the potential to be applied in a real benzene-contaminated site.

GRAPHICAL ABSTRACT

Graphical abstract

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors would like to thank King Mongkut's University of technology Thonburi through the ‘Kmutt 55th Anniversary Commemorative fund’ for financial support, National Research Council of Thailand and thank Assoc. Prof. Dr. Suvit Tia for providing the container for doing the experiment [Support for Remediation Laboratory].

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