Purpose: To describe a case of suprachoroidal hemorrhage occurring during 25-gauge vitrectomy. Methods: Retrospective case review. Results: An 80-year old pseudophakic man developed intraoperative suprachoroidal hemorrhage during a vitreous biopsy procedure for chronic intraocular inflammation. Despite drainage of the choroidals, visual outcome was poor. Conclusions: 25-gauge vitrectomy is often referred to as a “less-invasive” procedure than 20-gauge vitrectomy, but it is not necessarily less risky and probably carries a similar risk profile.