A 1.8 kb PstI fragment from Halobacterium halobium DNA was found to hybridize to synthetic oligonu-cleotide probes constructed by using the sequence of the N-terminus of a Mn-containing superoxide dismutase purified from H.halobium. The entire insert containing a 600-bp coding sequence for Mn-SOD and its 5′ and 3′ flanking regions was sequenced. The derived amino acid sequence of the structural gene showed a similarity to other manganic and iron-containing superoxide dismutases in normally conserved regions. Primer extension analysis of the H. halobium Mn-SOD mRNA showed that gene transcription begins 14 bases upstream of the translational start. A Shine-Dalgarno sequence and archaebacterial consensus promoter sequences were observed. Several other promoter and terminator nucleotide sequences homologous to prokaryotic and eukaryotic organisms were found.
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Free Radical Research Communications
Volume 12, 1991 - Issue 1
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Original Article
Halobacterium Halobium Mn-Sod Gene: Archaebacterial and Eubacterial Features
Marvin L. Salin Department of Biochemistry, Molecular Biology, Mississippi State University, P.O. Drawer BB, Mississippi State, MS, 39762, USA
, Mary V. Duke Department of Biochemistry, Molecular Biology, Mississippi State University, P.O. Drawer BB, Mississippi State, MS, 39762, USA
, Din-Pow Ma Department of Biochemistry, Molecular Biology, Mississippi State University, P.O. Drawer BB, Mississippi State, MS, 39762, USA
& John A. Boyle Department of Biochemistry, Molecular Biology, Mississippi State University, P.O. Drawer BB, Mississippi State, MS, 39762, USA
Pages 443-449
Published online: 07 Jul 2009
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Original Article
Halobacterium Halobium Mn-Sod Gene: Archaebacterial and Eubacterial Features
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