Background: Duodenal surface epithelial cells secrete bicarbonate. Agonists of duodenal alkaline secretion (such as vasoactive intestinal polypeptide (VIP), prostaglandin E2 (PGE2), and forskolin) increase intracellular cyclic adenosine-3′,5′-monophosphate (cAMP), and cAMP stimulates C-HCO3- exchange in duodenal brush border membrane vesicles. As intestinal villus and crypt cells differ in function, our aims were to contrast cAMP generation in duodenal villus versus crypt cells in response to VIP, PGE2, and forskolin. Methods: Villus and crypt rabbit duodenal enterocytes were isolated by calcium chelation. To prevent the degradation of cAMP in vitro, phosphodiesterase activity was inhibited. cAMP production was quantitated in response to VIP (10-10-10-5M), PGE2 (10-10-10-4M), and forskolin (10-8-10-3M). Results: In crypt cells cAMP generation was approximately 10-fold greater (P < 0.001) in response to VIP, PGE2, and forskolin than to villus cells. The relative orders of potency (that is, D50, VIP > PGE2 > forskolin) and efficacy (that is, Vmax, forskolin > VIP and PGE2) were similar in villus and crypt cells. Conclusion: cAMP production is greater in duodenal crypt than in villus enterocytes at rest and in response to forskolin, VIP, and PGE2, suggesting that alkaline secretion may differ along the villus-to-crypt axis.
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Scandinavian Journal of Gastroenterology
Volume 31, 1996 - Issue 3
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Original Article
Cyclic Adenosine-3′,5′-Monophosphate Production Is Greater in Rabbit Duodenal Crypt Than in Villus Cells
M. Amelsberg Division of Gastroenterology, Dept. of Medicine, University of California at San Diego, San Diego, California, USA
, A. Amelsberg Division of Gastroenterology, Dept. of Medicine, University of California at San Diego, San Diego, California, USA
, M. A. Ainsworth Division of Gastroenterology, Dept. of Medicine, University of California at San Diego, San Diego, California, USA
, D. L. Hogan Division of Gastroenterology, Dept. of Medicine, University of California at San Diego, San Diego, California, USA
& J. I. Isenberg Division of Gastroenterology, Dept. of Medicine, University of California at San Diego, San Diego, California, USA
Pages 233-239
Received 28 Jun 1995
Accepted 27 Aug 1995
Published online: 08 Jul 2009
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Original Article
Cyclic Adenosine-3′,5′-Monophosphate Production Is Greater in Rabbit Duodenal Crypt Than in Villus Cells
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