Photochemical attachment of synthetic oligonucleotides on the three dimensional surface of a polyacrylamide based hydrogel was used in the specific detection of target oligonucleotides. Covalent attachment of the oligonucleotide to the hydrogel was mediated by the incorporation of a 2+2 photo-attachable functional group in both the hydrogel and the oligonucleotide probe. Expression and SNP assays were used to evaluate this platform.
ACKNOWLEDGMENTS
We would like to thank Dr. Kurt Meier of HighTech Photopolymere AG, Basel, Switzerland for valuable input in the design and synthesis of the DMI polymer.
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