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Animal Biotechnology

Volume 16, 2005 - Issue 2
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Miscellany

Nucleotide Sequence and Variation of IGF2 Gene Exon 6 in Bos Taurus and Bos Indicus Cattle

Krzysztof Flisikowski Institute of Genetics and Animal Breeding, Jastrzebiec, Wolka Kosowska, Poland
,
Andrzej Maj Institute of Genetics and Animal Breeding, Jastrzebiec, Wolka Kosowska, Poland
,
Lech Zwierzchowski Institute of Genetics and Animal Breeding, Jastrzebiec, Wolka Kosowska, Poland
,
Tatiana Adamowicz August Cieszkowski Agricultural University, Department of Genetics and Animal Breeding, Poznan, Poland
,
Marek Switonski August Cieszkowski Agricultural University, Department of Genetics and Animal Breeding, Poznan, Poland
,
Stefan Hiendleder Department of Molecular Animal Breeding and Biotechnology, Gene Center of the Ludwig-Maximilian University, Munich, Germany
&
Chandra Pareek University of Warmia and Mazury, Department of Animal Genetics, Olsztyn, Poland
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Pages 203-208
Published online: 06 Jan 2011
 
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Miscellany

Nucleotide Sequence and Variation of IGF2 Gene Exon 6 in Bos Taurus and Bos Indicus Cattle

Abstract

The major assumption of this study is that polymorphism of a gene could be used to investigate its allele-specific expression as well as its methylation and imprinting status. Therefore, the aim of this study was to analyze the polymorphism of the coding region of the bovine IGF2 gene and to determine the sequence of its gene exon 6 in Bos taurus and Bos indicus cattle. A single nucleotide “C” deletion/insertion polymorphism was found in both cattle subspecies and a G/T transversion (RFLP-MboII) in the Bos indicus IGF2 gene. A 407-bp fragment of bovine IGF2 exon 6 was sequenced and the sequences (including variable nucleotides) were deposited in the GenBank database. A comparative analysis was performed for this fragment from different species; 99.5% identity was found between Bos taurus and Bos indicus cattle.

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