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Original Articles

A General Procedure for Assigning the 31P Spectra of Drug-Oligonucleotide Complexes

, , &
Pages 933-937
Received 05 Nov 1987
Published online: 15 May 2012

Abstract

Taking advantage of the slow exchange at the NMR time scale occurring in drug oligonucleotides complexes the 3P signals in the bound forms are assigned by using 31PNMR two dimensional chemical exchange. This technique was applied to complexes between Actinomycin D and d[CpGpCpG] or d[m5CpGpm 5CpG], As compared to the labelled 17O,18O this method proved to be a powerful and unique way to assign 31P in broad spectrum or with long oligonucleotides.

 

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