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Preparative Biochemistry

Volume 5, 1975 - Issue 2
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Original Articles

Fractionation of Some Bovine Whey Proteins by Recycling Gel Filtration on a Large Scale

Ake Ljungquist Mjölkcentralen, Ekonomisk Förening, Dalagatan 3 , S-111 23, STOCKHOLM, Sweden ; Pharmacia Fine Chemicals AB , Box 175, S-751 04, UPPSALA 1, Sweden
,
Lars-Olov Lindqvist Mjölkcentralen, Ekonomisk Förening, Dalagatan 3 , S-111 23, STOCKHOLM, Sweden ; Pharmacia Fine Chemicals AB , Box 175, S-751 04, UPPSALA 1, Sweden
&
Per Wallin Mjölkcentralen, Ekonomisk Förening, Dalagatan 3 , S-111 23, STOCKHOLM, Sweden ; Pharmacia Fine Chemicals AB , Box 175, S-751 04, UPPSALA 1, Sweden
Pages 131-146
Published online: 06 Dec 2006
 
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Original Articles

Fractionation of Some Bovine Whey Proteins by Recycling Gel Filtration on a Large Scale

Abstract

Abstract

Fractionation of bovine whey concentrate was performed by gel filtration on Sephadex G-75 both on a laboratory scale and on a large scale. By a recycling procedure an improved separation was obtained and the whey proteins were resolved into four fractions in the weight ratio 3:12:1:4.

The fractions were analysed by polyacrylamide gel (PAG) electrophoresis and the apparent molecular weights were determined by thin layer gel chromatography (TLG) and by sodium dode-cylsulfate (SDS) gel electrophoresis.

 

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