Cart
Identification of potential biomarkers for diazinon exposure to Japanese Medaka (Oryzias latipes) using annealing control primers
- DOI:
- 10.1080/03601230701310526
pages 373-379
Available online: 03 May 2007Abstract
A new differential display-polymerase chain reaction (PCR) method based on annealing control primers was used to screen and identify potential biomarkers from differentially expressed genes (DEGs) in medaka exposed to sub-lethal concentration of diazinon (100 ppb). Among the differentially expressed genes identified, the majority were in functional categories of protein biosynthesis, transport and metabolism according to the gene ontology classification. The differential expression of ribosomal protein genes was quantified by real time PCR. The genes encoding ribosomal proteins including L3 and S17 were selected as potential biomarkers for diazinon exposure in medaka fish.
Keywords
- Recommend to:
- A friend
First page preview
Details
- Citation information:
- Available online: 03 May 2007
Author affiliations
- a Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
- b Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
- c Department of Chemical Bio-engineering, Kyungwon University, Sungnam, Korea
- d Division of Civil and Environmental Engineering, Korea Maritime University, Busan, Korea
- e Hankuk Academy of Foreign Studies, Yongin, Korea
Journal news
- Increasing to 10 issues in 2012
Librarians
- Librarians' area
- Pricing
- Institutional account
- Access entitlements
- Co-branding
- IP ranges
- Link resolver preferences
- Usage reports